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ELL2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ELL2
Gene Name:  elongation factor, RNA polymerase II, 2
Sequence             
Original Clone ID: FLH269162.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329427

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1923nts         Open reading frame : 1 to 1923
Reference Sequence Alignment

ATGGCGGCGGGGGGGACAGGGGGCCTGCGGGAGGAGCAGCGCTATGGGCTGTCGTGCGGA
CGGCTGGGGCAGGACAACATCACCGTACTGCATGTGAAGCTCACCGAGACGGCGATCCGG
GCGCTCGAGACTTACCAGAGCCACAAGAATTTAATTCCTTTTCGACCTTCAATCCAGTTC
CAAGGACTCCACGGGCTTGTCAAAATTCCCAAAAATGATCCCCTCAATGAAGTTCATAAC
TTTAACTTTTATTTGTCAAATGTGGGCAAAGACAACCCTCAGGGCAGCTTTGACTGCATC
CAGCAAACATTCTCCAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATTTATACAAGAT
AAAATTACAGTGTGTGCAACAAACGACTCGTATCAGATGACACGAGAAAGAATGACCCAG
GCAGAGGAGGAATCCCGCAACCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTA
GGGAAAAGAGTGCAAATTCGGAAAGCACCTCAAGCTGTTTCAGATACAGTTCCTGAGAGG
AAAAGGTCAACCCCCATGAACCCTGCAAATACAATTCGAAAGACACATAGCAGCAGCACC
ATCTCTCAGAGGCCATACAGGGACAGGGTGATTCACTTACTGGCCCTGAAGGCCTACAAG
AAACCGGAGCTACTTGCTAGACTCCAGAAAGATGGTGTCAATCAAAAAGACAAGAACTCC
CTGGGAGCAATTCTGCAACAGGTAGCCAATCTGAATTCTAAGGACCTCTCATATACCTTA
AAGGATTATGTTTTTAAAGAGCTTCAAAGAGACTGGCCTGGATACAGTGAAATAGACAGA
CGGTCATTGGAGTCAGTGCTCTCTAGAAAACTAAATCCGTCTCAGAATGCTACAGGCACC
AGCCGTTCAGAATCTCCTGTATGTTCTAGTAGAGATGCTGTATCTTCTCCTCAGAAACGG
CTTTTGGATTCAGAGTTTATTGATCCTTTAATGAATAAAAAAGCCCGAATATCTCACCTG
ACGAACAGAGTACCACCAACACTAAATGGTCATTTGAATCCCACCAGTGAAAAATCGGCT
GCAGGCCTCCCACTGCCCCCTGCGGCTGCTGCCATCCCCACCCCTCCACCGCTGCCTTCA
ACCTATCTGCCCATCTCACATCCTCCTCAGATTGTAAATTCTAACTCCAACTCCCCTAGC
ACTCCAGAAGGCCGGGGGACTCAAGACCTACCTGTTGACAGTTTTAGTCAAAACGATAGT
ATCTATGAGGACCAGCAAGACAAATATACCTCTAGGACTTCTCTGGAAACCTTACCCCCT
GGTTCCGTTCTACTAAAGTGTCCAAAGCCTATGGAAGAAAACCATTCAATGTCTCACAAA
AAGTCCAAAAAGAAGTCTAAAAAACATAAGGAAAAGGACCAAATAAAAAAGCACGACATT
GAGACTATTGAGGAAAAGGAGGAAGATCTTAAGAGAGAAGAGGAAATTGCCAAGCTAAAT
AACTCCAGTCCAAATTCCAGTGGAGGAGTTAAAGAGGATTGCACTGCCTCCATGGAACCT
TCAGCAATTGAACTCCCAGATTATTTGATAAAATATATCGCTATCGTCTCCTATGAGCAA
CGCCAGAATTATAAGGATGACTTCAATGCAGAGTATGATGAGTACAGAGCTTTGCATGCC
AGGATGGAGACTGTAGCTAGAAGATTTATCAAACTAGATGCACAAAGAAAGCGCCTTTCT
CCAGGCTCAAAAGAGTATCAGAATGTTCATGAAGAAGTCTTACAAGAATATCAGAAGATA
AAGCAGTCTAGTCCCAATTACCATGAAGAAAAATACAGATGTGAATATCTTCATAACAAG
CTGGCTCACATCAAAAGGCTAATAGGTGAATTTGACCAACAGCAAGCAGAGTCATGGTCC
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2280
Start on reference sequence 358
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ELL2
Symbol Nomenclature : ELL2
Designation : ELL-related RNA polymerase II, elongation factor|RNA polymerase II elongation factor ELL2
Full Nomenclature : elongation factor, RNA polymerase II, 2
GENEID : 22936
HGNC : 17064
MIM : 601874
Vega : OTTHUMG00000122085
Target GenBank: BC028412

 

Reference Sequence Alignment

  

HsCD00329427      ATGGCGGCGGGGGGGACAGGGGGCCTGCGGGAGGAGCAGCGCTATGGGCTGTCGTGCGGA
NM_012081.5       ATGGCGGCGGGGGGGACAGGGGGCCTGCGGGAGGAGCAGCGCTATGGGCTGTCGTGCGGA
                  ************************************************************

HsCD00329427      CGGCTGGGGCAGGACAACATCACCGTACTGCATGTGAAGCTCACCGAGACGGCGATCCGG
NM_012081.5       CGGCTGGGGCAGGACAACATCACCGTACTGCATGTGAAGCTCACCGAGACGGCGATCCGG
                  ************************************************************

HsCD00329427      GCGCTCGAGACTTACCAGAGCCACAAGAATTTAATTCCTTTTCGACCTTCAATCCAGTTC
NM_012081.5       GCGCTCGAGACTTACCAGAGCCACAAGAATTTAATTCCTTTTCGACCTTCAATCCAGTTC
                  ************************************************************

HsCD00329427      CAAGGACTCCACGGGCTTGTCAAAATTCCCAAAAATGATCCCCTCAATGAAGTTCATAAC
NM_012081.5       CAAGGACTCCACGGGCTTGTCAAAATTCCCAAAAATGATCCCCTCAATGAAGTTCATAAC
                  ************************************************************

HsCD00329427      TTTAACTTTTATTTGTCAAATGTGGGCAAAGACAACCCTCAGGGCAGCTTTGACTGCATC
NM_012081.5       TTTAACTTTTATTTGTCAAATGTGGGCAAAGACAACCCTCAGGGCAGCTTTGACTGCATC
                  ************************************************************

HsCD00329427      CAGCAAACATTCTCCAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATTTATACAAGAT
NM_012081.5       CAGCAAACATTCTCCAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATTTATACAAGAT
                  ************************************************************

HsCD00329427      AAAATTACAGTGTGTGCAACAAACGACTCGTATCAGATGACACGAGAAAGAATGACCCAG
NM_012081.5       AAAATTACAGTGTGTGCAACAAACGACTCGTATCAGATGACACGAGAAAGAATGACCCAG
                  ************************************************************

HsCD00329427      GCAGAGGAGGAATCCCGCAACCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTA
NM_012081.5       GCAGAGGAGGAATCCCGCAACCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTA
                  ************************************************************

HsCD00329427      GGGAAAAGAGTGCAAATTCGGAAAGCACCTCAAGCTGTTTCAGATACAGTTCCTGAGAGG
NM_012081.5       GGGAAAAGAGTGCAAATTCGGAAAGCACCTCAAGCTGTTTCAGATACAGTTCCTGAGAGG
                  ************************************************************

HsCD00329427      AAAAGGTCAACCCCCATGAACCCTGCAAATACAATTCGAAAGACACATAGCAGCAGCACC
NM_012081.5       AAAAGGTCAACCCCCATGAACCCTGCAAATACAATTCGAAAGACACATAGCAGCAGCACC
                  ************************************************************

HsCD00329427      ATCTCTCAGAGGCCATACAGGGACAGGGTGATTCACTTACTGGCCCTGAAGGCCTACAAG
NM_012081.5       ATCTCTCAGAGGCCATACAGGGACAGGGTGATTCACTTACTGGCCCTGAAGGCCTACAAG
                  ************************************************************

HsCD00329427      AAACCGGAGCTACTTGCTAGACTCCAGAAAGATGGTGTCAATCAAAAAGACAAGAACTCC
NM_012081.5       AAACCGGAGCTACTTGCTAGACTCCAGAAAGATGGTGTCAATCAAAAAGACAAGAACTCC
                  ************************************************************

HsCD00329427      CTGGGAGCAATTCTGCAACAGGTAGCCAATCTGAATTCTAAGGACCTCTCATATACCTTA
NM_012081.5       CTGGGAGCAATTCTGCAACAGGTAGCCAATCTGAATTCTAAGGACCTCTCATATACCTTA
                  ************************************************************

HsCD00329427      AAGGATTATGTTTTTAAAGAGCTTCAAAGAGACTGGCCTGGATACAGTGAAATAGACAGA
NM_012081.5       AAGGATTATGTTTTTAAAGAGCTTCAAAGAGACTGGCCTGGATACAGTGAAATAGACAGA
                  ************************************************************

HsCD00329427      CGGTCATTGGAGTCAGTGCTCTCTAGAAAACTAAATCCGTCTCAGAATGCTACAGGCACC
NM_012081.5       CGGTCATTGGAGTCAGTGCTCTCTAGAAAACTAAATCCGTCTCAGAATGCTGCAGGCACC
                  ***************************************************.********

HsCD00329427      AGCCGTTCAGAATCTCCTGTATGTTCTAGTAGAGATGCTGTATCTTCTCCTCAGAAACGG
NM_012081.5       AGCCGTTCAGAATCTCCTGTATGTTCTAGTAGAGACGCTGTATCTTCTCCTCAGAAACGG
                  *********************************** ************************

HsCD00329427      CTTTTGGATTCAGAGTTTATTGATCCTTTAATGAATAAAAAAGCCCGAATATCTCACCTG
NM_012081.5       CTTTTGGATTCAGAGTTTATTGATCCTTTAATGAATAAAAAAGCCCGAATATCTCACCTG
                  ************************************************************

HsCD00329427      ACGAACAGAGTACCACCAACACTAAATGGTCATTTGAATCCCACCAGTGAAAAATCGGCT
NM_012081.5       ACGAACAGAGTACCACCAACACTAAATGGTCATTTGAATCCCACCAGTGAAAAATCTGCT
                  ******************************************************** ***

HsCD00329427      GCAGGCCTCCCACTGCCCCCTGCGGCTGCTGCCATCCCCACCCCTCCACCGCTGCCTTCA
NM_012081.5       GCAGGCCTCCCGCTGCCCCCTGCGGCTGCTGCCATCCCTACCCCTCCACCGCTGCCTTCA
                  ***********.************************** *********************

HsCD00329427      ACCTATCTGCCCATCTCACATCCTCCTCAGATTGTAAATTCTAACTCCAACTCCCCTAGC
NM_012081.5       ACCTATCTGCCCATCTCACATCCTCCTCAGATTGTAAATTCTAACTCCAACTCCCCTAGC
                  ************************************************************

HsCD00329427      ACTCCAGAAGGCCGGGGGACTCAAGACCTACCTGTTGACAGTTTTAGTCAAAACGATAGT
NM_012081.5       ACTCCAGAAGGCCGGGGGACTCAAGACCTACCTGTTGACAGTTTTAGTCAAAACGATAGT
                  ************************************************************

HsCD00329427      ATCTATGAGGACCAGCAAGACAAATATACCTCTAGGACTTCTCTGGAAACCTTACCCCCT
NM_012081.5       ATCTATGAGGACCAGCAAGACAAATATACCTCTAGGACTTCTCTGGAAACCTTACCCCCT
                  ************************************************************

HsCD00329427      GGTTCCGTTCTACTAAAGTGTCCAAAGCCTATGGAAGAAAACCATTCAATGTCTCACAAA
NM_012081.5       GGTTCCGTTCTACTAAAGTGTCCAAAGCCTATGGAAGAAAACCATTCAATGTCTCACAAA
                  ************************************************************

HsCD00329427      AAGTCCAAAAAGAAGTCTAAAAAACATAAGGAAAAGGACCAAATAAAAAAGCACGACATT
NM_012081.5       AAGTCCAAAAAGAAGTCTAAAAAACATAAGGAAAAGGACCAAATAAAAAAGCACGACATT
                  ************************************************************

HsCD00329427      GAGACTATTGAGGAAAAGGAGGAAGATCTTAAGAGAGAAGAGGAAATTGCCAAGCTAAAT
NM_012081.5       GAGACTATTGAGGAAAAGGAGGAAGATCTTAAGAGAGAAGAGGAAATTGCCAAGCTAAAT
                  ************************************************************

HsCD00329427      AACTCCAGTCCAAATTCCAGTGGAGGAGTTAAAGAGGATTGCACTGCCTCCATGGAACCT
NM_012081.5       AACTCCAGTCCAAATTCCAGTGGAGGAGTTAAAGAGGATTGCACTGCCTCCATGGAACCT
                  ************************************************************

HsCD00329427      TCAGCAATTGAACTCCCAGATTATTTGATAAAATATATCGCTATCGTCTCCTATGAGCAA
NM_012081.5       TCAGCAATTGAACTCCCAGATTATTTGATAAAATATATCGCTATCGTCTCCTATGAGCAA
                  ************************************************************

HsCD00329427      CGCCAGAATTATAAGGATGACTTCAATGCAGAGTATGATGAGTACAGAGCTTTGCATGCC
NM_012081.5       CGCCAGAATTATAAGGATGACTTCAATGCAGAGTATGATGAGTACAGAGCTTTGCATGCC
                  ************************************************************

HsCD00329427      AGGATGGAGACTGTAGCTAGAAGATTTATCAAACTAGATGCACAAAGAAAGCGCCTTTCT
NM_012081.5       AGGATGGAGACTGTAGCTAGAAGATTTATCAAACTAGATGCACAAAGAAAGCGCCTTTCT
                  ************************************************************

HsCD00329427      CCAGGCTCAAAAGAGTATCAGAATGTTCATGAAGAAGTCTTACAAGAATATCAGAAGATA
NM_012081.5       CCAGGCTCAAAAGAGTATCAGAATGTTCATGAAGAAGTCTTACAAGAATATCAGAAGATA
                  ************************************************************

HsCD00329427      AAGCAGTCTAGTCCCAATTACCATGAAGAAAAATACAGATGTGAATATCTTCATAACAAG
NM_012081.5       AAGCAGTCTAGTCCCAATTACCATGAAGAAAAATACAGATGTGAATATCTTCATAACAAG
                  ************************************************************

HsCD00329427      CTGGCTCACATCAAAAGGCTAATAGGTGAATTTGACCAACAGCAAGCAGAGTCATGGTCC
NM_012081.5       CTGGCTCACATCAAAAGGCTAATAGGTGAATTTGACCAACAGCAAGCAGAGTCATGGTCC
                  ************************************************************

HsCD00329427      TTG
NM_012081.5       TAG
                  *:*

Protein

UniProt : Q59FW6
UniProt : O00472
UniProt : Q7Z656
HPRD : 11798

Clone

Cloning Information : 269162
HIP Master Clone ID : 152964
Original Clone ID : FLH269162.01L

Organism


TAX_ID : 9606
Species Specific ID: 22936

Genome

Chromosome : 5
Map Location : 5q15
Ensembl : ENSG00000118985

Reagents

Labome : ELL2-antibody