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TGIF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TGIF1
Gene Name:  TGFB-induced factor homeobox 1
Sequence             
Original Clone ID: FLH269163.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329428

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1206nts         Open reading frame : 1 to 1206
Reference Sequence Alignment

ATGGTTCTAGCGCAGAGCCGGGTGTCTGCCGGGGTGGGCTCCCCGCATTGTTCGGGCTCC
GGCGGGGGCGGCTCTGATTCCTTTCCATGGCCCGCCTCCCACCCCGGGAATCCGCAGTGC
TCCTTTTCCACGGCTTTTCTGGCGTCCCCCCGACTCTCCCGCGGCACTTTGGCCTACCTT
CCCCCAGCGCCGTGGTCCTCCCTGGCGACCCCCTCTGCGCTCCTGGGGTCCTCCTGCGCC
CCCCCTCCTCCACCGGCGCGCTGCCCACAGCCGCGTGCCCTCTCCCAGGAGCTGGGGACC
AAGGCTGGGCCCCGCCGGCCGCATCGGTGGGAACTTCCGCGGTCCCCATCCCAGGGCGCA
CAGGGTCCAGCTCCTCGGCGCCGACTCCTGGAAACAATGAAAGGTATTGTTGCAGCATCT
GGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCT
GGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGATTCTTCGG
GATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTG
TCCCAGCAAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGC
AGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACAATTTCC
CGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAAC
TTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAGCTGGGCCAAACCCAACC
CTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCA
GTGATCTGCCATACCACTGTGACTGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCG
GTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAACTTCACAGACACC
TCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGT
CTTTTCAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAG
CTTCTAGTGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACA
GCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1509
Start on reference sequence 304
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TGIF1
Symbol Nomenclature : TGIF1
SYNONYM : HPE4; TGIF
Designation : 5'-TG-3'-interacting factor 1|TALE homeobox TG-interacting factor|homeobox protein TGIF1|transforming growth factor-beta-induced factor
Full Nomenclature : TGFB-induced factor homeobox 1
GENEID : 7050
HGNC : 11776
MIM : 602630
Vega : OTTHUMG00000131511
Target GenBank: BC031268

 

Reference Sequence Alignment

  

HsCD00329428      ATGGTTCTAGCGCAGAGCCGGGTGTCTGCCGGGGTGGGCTCCCCGCATTGTTCGGGCTCC
NM_170695.3       ATGGTTCTAGCGCAGAGCCGGGTGTCTGCCGGGGTGGGCTCCCCGCATTGTTCGGGCTCC
                  ************************************************************

HsCD00329428      GGCGGGGGCGGCTCTGATTCCTTTCCATGGCCCGCCTCCCACCCCGGGAATCCGCAGTGC
NM_170695.3       GGCGGGGGCGGCTCTGATTCCTTTCCATGGCCCGCCTCCCACCCCGGGAATCCCCAGTGC
                  ***************************************************** ******

HsCD00329428      TCCTTTTCCACGGCTTTTCTGGCGTCCCCCCGACTCTCCCGCGGCACTTTGGCCTACCTT
NM_170695.3       TCCTTTTCCACGGCTTTTCTGGCGTCCCCCCGACTCTCCCGCGGCACTTTGGCCTACCTT
                  ************************************************************

HsCD00329428      CCCCCAGCGCCGTGGTCCTCCCTGGCGACCCCCTCTGCGCTCCTGGGGTCCTCCTGCGCC
NM_170695.3       CCCCCAGCGCCGTGGTCCTCCCTGGCGACCCCCTCTGCGCTCCTGGGGTCCTCCTGCGCC
                  ************************************************************

HsCD00329428      CCCCCTCCTCCACCGGCGCGCTGCCCACAGCCGCGTGCCCTCTCCCAGGAGCTGGGGACC
NM_170695.3       CCCCCTCCTCCACCGGCGCGCTGCCCACAGCCGCGTGCCCTCTCCCCGGAGCTGGGGACC
                  **********************************************.*************

HsCD00329428      AAGGCTGGGCCCCGCCGGCCGCATCGGTGGGAACTTCCGCGGTCCCCATCCCAGGGCGCA
NM_170695.3       AAGGCTGGGCCCCGCCGGCCGCATCGGTGGGAACTTCCGCGGTCCCCATCCCAGGGCGCA
                  ************************************************************

HsCD00329428      CAGGGTCCAGCTCCTCGGCGCCGACTCCTGGAAACAATGAAAGGTATTGTTGCAGCATCT
NM_170695.3       CAGGGTCCAGCTCCTCGGCGCCGACTCCTGGAAACAATGAAAGGTATTGTTGCAGCATCT
                  ************************************************************

HsCD00329428      GGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCT
NM_170695.3       GGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCT
                  ************************************************************

HsCD00329428      GGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGATTCTTCGG
NM_170695.3       GGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGATTCTTCGG
                  ************************************************************

HsCD00329428      GATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTG
NM_170695.3       GATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTG
                  ************************************************************

HsCD00329428      TCCCAGCAAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGC
NM_170695.3       TCCCAGCAAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGC
                  ************************************************************

HsCD00329428      AGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACAATTTCC
NM_170695.3       AGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACAATTTCC
                  ************************************************************

HsCD00329428      CGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAAC
NM_170695.3       CGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAAC
                  ************************************************************

HsCD00329428      TTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAGCTGGGCCAAACCCAACC
NM_170695.3       TTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAGCTGGGCCAAACCCAACC
                  ************************************************************

HsCD00329428      CTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCA
NM_170695.3       CTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCA
                  ************************************************************

HsCD00329428      GTGATCTGCCATACCACTGTGACTGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCG
NM_170695.3       GTGATCTGCCATACCACTGTGACTGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCG
                  ************************************************************

HsCD00329428      GTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAACTTCACAGACACC
NM_170695.3       GTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAACTTCACAGACACC
                  ************************************************************

HsCD00329428      TCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGT
NM_170695.3       TCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGT
                  ************************************************************

HsCD00329428      CTTTTCAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAG
NM_170695.3       CTTTTCAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAG
                  ************************************************************

HsCD00329428      CTTCTAGTGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACA
NM_170695.3       CTTCTAGTGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACA
                  ************************************************************

HsCD00329428      GCTTTG
NM_170695.3       GCTTAA
                  ****:.

Pathways

NCI : AP-1 transcription factor network
NCI : IL4-mediated signaling events
NCI : Regulation of nuclear SMAD2/3 signaling
Panther : Interleukin signaling pathway
WikiPathway : Cytokines and Inflammatory Response
WikiPathway : SIDS Susceptibility Pathways
WikiPathway : Th1/Th2

Protein

SMART domain : HOX : Homeodomain
UniProt : Q6FGW4
UniProt : P22301
HPRD : 04023

Clone

Cloning Information : 269163
HIP Master Clone ID : 153239
Original Clone ID : FLH269163.01L

Organism


TAX_ID : 9606
Species Specific ID: 3586

Genome

Chromosome : 18
Map Location : 18p11.3
Ensembl : ENSG00000177426

Reagents

Labome : TGIF-antibody