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THRAP6 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MED30
Gene Name:  mediator complex subunit 30
Sequence             
Original Clone ID: FLH269165.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329430

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 537nts         Open reading frame : 1 to 537
Reference Sequence Alignment

ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAG
GCTCAGCAGGCCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACA
GTGCAGGACATCGTGTACCGCACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTG
CCAAATGGTGTCACTTACCACACTGGAACATATCAAGACCGGTTAACAAAGCTACAGGAT
AATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGATTGGTATATGACAAATGCAAT
GAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATATGTGGAAGAA
GATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA
GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATG
GATCAATTACGAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 623
Start on reference sequence 87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MED30
Symbol Nomenclature : MED30
SYNONYM : MED30S; THRAP6; TRAP25
Designation : TRAP/Mediator complex component TRAP25|mediator of RNA polymerase II transcription subunit 30|putative mediator of RNA polymerase II transcription subunit 30|thyroid hormone receptor associated protein 6|thyroid hormone receptor-associated protein 6|thyroid hormone receptor-associated protein complex 25 kDa component
Full Nomenclature : mediator complex subunit 30
GENEID : 90390
HGNC : 23032
MIM : 610237
Vega : OTTHUMG00000164920
Target GenBank: BC008226

 

Reference Sequence Alignment

  

HsCD00329430      ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAG
NM_080651.3       ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAG
                  ************************************************************

HsCD00329430      GCTCAGCAGGCCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACA
NM_080651.3       GCTCAGCAGGCCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACA
                  ************************************************************

HsCD00329430      GTGCAGGACATCGTGTACCGCACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTG
NM_080651.3       GTGCAGGACATCGTGTACCGCACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTG
                  ************************************************************

HsCD00329430      CCAAATGGTGTCACTTACCACACTGGAACATATCAAGACCGGTTAACAAAGCTACAGGAT
NM_080651.3       CCAAATGGTGTCACTTACCACACTGGAACATATCAAGACCGGTTAACAAAGCTACAGGAT
                  ************************************************************

HsCD00329430      AATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGATTGGTATATGACAAATGCAAT
NM_080651.3       AATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGATTGGTATATGACAAATGCAAT
                  ************************************************************

HsCD00329430      GAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATATGTGGAAGAA
NM_080651.3       GAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATATGTGGAAGAA
                  ************************************************************

HsCD00329430      GATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA
NM_080651.3       GATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA
                  ************************************************************

HsCD00329430      GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATG
NM_080651.3       GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATG
                  ************************************************************

HsCD00329430      GATCAATTACGAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAACTTG
NM_080651.3       GATCAATTACGAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAACTAA
                  *******************************************************:.

Pathways

Reactome : Developmental Biology
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : PPARA activates gene expression
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
Reactome : Transcriptional regulation of white adipocyte differentiation

Protein

UniProt : Q96HR3
HPRD : 11631

Clone

Cloning Information : 269165
HIP Master Clone ID : 152802
Original Clone ID : FLH269165.01L

Organism


TAX_ID : 9606
Species Specific ID: 90390

Genome

Chromosome : 8
Map Location : 8q24.11
Ensembl : ENSG00000164758

Reagents

Labome : MED30-antibody