DNASU Plasmid Repository • 480.965.5697 | Email

HIST1H4F (H. sapiens ) in pLenti6.2/V5-DEST

TOP

Explanation of Terms


Gene: Gene Symbol:  HIST2H4B
Gene Name:  histone cluster 2, H4b
Sequence             
Original Clone ID: FLH269169.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329434

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 312nts         Open reading frame : 1 to 312
Reference Sequence Alignment

ATGTCTGGCCGCGGCAAAGGCGGGAAGGGTCTTGGCAAAGGCGGCGCTAAGCGCCACCGT
AAAGTACTGCGCGACAATATCCAGGGCATCACCAAGCCGGCCATCCGGCGCCTTGCTCGC
CGCGGCGGCGTGAAGCGCATCTCCGGCCTCATCTACGAGGAGACTCGCGGGGTGCTGAAG
GTGTTCCTGGAGAACGTGATCCGGGACGCCGTGACCTATACAGAGCACGCCAAGCGCAAG
ACGGTCACCGCCATGGATGTGGTCTACGCGCTCAAGCGCCAGGGCCGCACCCTCTACGGT
TTCGGTGGTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 340
Start on reference sequence 29
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HIST2H4B
Symbol Nomenclature : HIST2H4B
SYNONYM : H4/o
Designation : histone 2, H4b|histone H4
Full Nomenclature : histone cluster 2, H4b
GENEID : 554313
HGNC : 29607
Vega : OTTHUMG00000012084
Target GenBank: BC017361

 

Reference Sequence Alignment

  

HsCD00329434        ATGTCTGGCCGCGGCAAAGGCGGGAAGGGTCTTGGCAAAGGCGGCGCTAAGCGCCACCGT
NM_003495.2         ATGTCAGGACGCGGCAAAGGAGGTAAGGGCCTGGGGAAAGGGGGTGCCAAGCGCCACCGC
NM_003545.3         ATGTCTGGTCGCGGCAAAGGCGGAAAGGGACTGGGTAAAGGAGGCGCTAAGCGTCACCGT
NM_003543.3         ATGTCTGGCCGTGGTAAAGGTGGAAAAGGTTTGGGTAAGGGAGGAGCTAAGCGTCATCGC
NM_003539.3         ATGTCTGGCCGCGGTAAGGGCGGAAAGGGTCTAGGTAAGGGTGGCGCCAAGCGTCACCGT
NM_003538.3         ATGTCTGGACGTGGTAAGGGCGGGAAGGGTTTGGGTAAGGGGGGTGCCAAGCGCCACCGC
NM_021968.3         ATGTCTGGCCGCGGCAAAGGCGGGAAGGGTCTTGGCAAAGGCGGCGCTAAGCGCCACCGT
NM_003546.2         ATGTCTGGGCGCGGCAAAGGCGGGAAGGGTCTGGGCAAAGGAGGCGCTAAGCGCCACCGC
NM_003544.2         ATGTCTGGTCGCGGCAAAGGCGGTAAAGGTTTGGGTAAGGGAGGTGCCAAGCGTCACCGA
NM_001034077.4      ATGTCCGGCAGAGGAAAGGGCGGAAAAGGCTTAGGCAAAGGGGGCGCTAAGCGCCACCGC
NM_003548.2         ATGTCCGGCAGAGGAAAGGGCGGAAAAGGCTTAGGCAAAGGGGGCGCTAAGCGCCACCGC
NM_003542.3         ATGTCTGGTCGCGGCAAAGGCGGAAAAGGCTTGGGGAAGGGTGGTGCTAAGCGCCATCGT
NM_175054.2         ATGTCTGGGCGAGGTAAAGGTGGCAAGGGGCTGGGTAAGGGAGGCGCCAAGCGCCACCGG
NM_003540.3         ATGTCTGGTAGAGGCAAAGGTGGTAAAGGTTTAGGAAAGGGAGGCGCCAAGCGCCATCGC
NM_003541.2         ATGTCTGGCCGCGGCAAAGGCGGGAAGGGTCTTGGCAAAGGCGGCGCTAAGCGCCACCGT
                    ***** ** .* ** **.** ** **.**  * ** **.** ** ** ***** ** ** 

HsCD00329434        AAAGTACTGCGCGACAATATCCAGGGCATCACCAAGCCGGCCATCCGGCGCCTTGCTCGC
NM_003495.2         AAGGTGCTGCGCGACAACATCCAGGGTATCACCAAGCCAGCCATTCGGCGCCTTGCTCGC
NM_003545.3         AAGGTCCTGCGAGATAACATCCAGGGCATTACCAAGCCTGCCATCCGGCGCCTTGCTCGT
NM_003543.3         AAGGTTTTGCGCGATAACATCCAGGGCATCACTAAGCCAGCTATCCGGCGCCTTGCTCGT
NM_003539.3         AAGGTATTGCGTGACAATATCCAAGGAATCACCAAGCCCGCTATCCGCCGCCTGGCTCGC
NM_003538.3         AAGGTGTTGCGTGACAACATCCAGGGCATCACCAAGCCGGCCATCCGGCGTCTGGCCCGG
NM_021968.3         AAAGTACTGCGCGACAATATCCAGGGCATCACCAAGCCGGCCATCCGGCGCCTTGCTCGC
NM_003546.2         AAAGTTCTGCGCGACAACATTCAGGGCATCACCAAGCCCGCCATCCGACGCCTGGCACGG
NM_003544.2         AAAGTGCTGCGGGATAACATCCAAGGCATCACCAAACCGGCCATTCGGCGCCTTGCTAGG
NM_001034077.4      AAGGTCTTGAGAGACAACATTCAGGGCATCACCAAGCCTGCCATTCGGCGTCTAGCTCGG
NM_003548.2         AAGGTCTTGAGAGACAACATTCAGGGCATCACCAAGCCTGCCATTCGGCGTCTAGCTCGG
NM_003542.3         AAGGTGCTCCGGGATAACATCCAGGGCATTACAAAACCGGCTATTCGCCGTTTGGCTCGG
NM_175054.2         AAGGTGCTGCGGGACAATATCCAAGGCATTACAAAGCCGGCGATTCGCCGTCTCGCCCGA
NM_003540.3         AAAGTGCTGCGTGACAACATACAGGGCATCACGAAGCCCGCCATCCGTCGCTTGGCCCGA
NM_003541.2         AAAGTACTGCGCGACAATATCCAGGGCATCACCAAGCCGGCCATCCGGCGCCTTGCTCGC
                    **.**  * .* ** ** ** **.** ** ** **.** ** ** ** **  * ** .* 

HsCD00329434        CGCGGCGGCGTGAAGCGCATCTCCGGCCTCATCTACGAGGAGACTCGCGGGGTGCTGAAG
NM_003495.2         CGCGGCGGCGTGAAGCGCATTTCTGGCCTCATCTATGAGGAGACCCGCGGAGTGTTGAAG
NM_003545.3         CGCGGGGGTGTCAAGCGCATTTCTGGTCTCATCTACGAGGAGACTCGCGGGGTTCTGAAG
NM_003543.3         CGCGGCGGTGTCAAGCGAATTTCTGGCCTTATCTATGAGGAGACTCGTGGTGTTCTGAAG
NM_003539.3         CGCGGCGGCGTCAAGCGTATTTCTGGCCTCATTTATGAGGAAACTCGCGGAGTGCTGAAA
NM_003538.3         CGTGGCGGTGTGAAGCGGATCTCTGGTCTGATCTACGAGGAGACTCGCGGGGTGCTCAAG
NM_021968.3         CGCGGCGGCGTGAAGCGCATCTCCGGCCTCATCTACGAGGAGACTCGCGGGGTGCTGAAG
NM_003546.2         CGTGGAGGCGTTAAGCGCATCTCAGGCCTTATATACGAGGAGACACGCGGAGTTCTTAAA
NM_003544.2         CGTGGTGGGGTTAAGCGAATTTCCGGTTTGATTTATGAGGAGACTCGTGGCGTTCTCAAG
NM_001034077.4      CGTGGCGGCGTTAAGCGGATCTCTGGCCTCATTTACGAGGAGACCCGCGGTGTGCTGAAG
NM_003548.2         CGTGGCGGCGTTAAGCGGATCTCTGGCCTCATTTACGAGGAGACCCGCGGTGTGCTGAAG
NM_003542.3         CGCGGTGGCGTCAAGCGCATTTCCGGTCTTATCTATGAGGAGACTCGAGGTGTGCTTAAG
NM_175054.2         CGTGGGGGCGTCAAGCGCATTTCTGGTCTCATCTACGAGGAGACCCGGGGAGTCCTCAAA
NM_003540.3         CGCGGCGGCGTGAAACGCATTTCGGGCCTCATTTATGAGGAGACCCGCGGTGTTCTTAAG
NM_003541.2         CGCGGCGGCGTGAAGCGCATCTCCGGCCTCATCTACGAGGAGACTCGCGGGGTGCTGAAG
                    ** ** ** ** **.** ** ** **  * ** ** *****.** ** ** **  * **.

HsCD00329434        GTGTTCCTGGAGAACGTGATCCGGGACGCCGTGACCTATACAGAGCACGCCAAGCGCAAG
NM_003495.2         GTGTTCCTGGAGAACGTGATCCGGGACGCCGTGACCTACACGGAGCACGCCAAGCGCAAG
NM_003545.3         GTGTTTCTGGAAAACGTGATTCGTGATGCTGTGACTTACACGGAGCACGCCAAACGCAAG
NM_003543.3         GTGTTCCTGGAGAACGTGATTCGTGACGCTGTCACTTACACAGAGCACGCCAAACGCAAG
NM_003539.3         GTTTTCCTGGAAAATGTAATCCGCGATGCTGTCACCTACACGGAACACGCCAAACGCAAG
NM_003538.3         GTGTTTTTGGAGAACGTGATCCGTGACGCTGTCACCTATACGGAGCACGCCAAGCGCAAG
NM_021968.3         GTGTTCCTGGAGAACGTGATCCGGGACGCCGTGACCTATACAGAGCACGCCAAGCGCAAG
NM_003546.2         GTGTTTTTGGAGAATGTAATCCGCGATGCAGTTACCTACACGGAGCACGCCAAACGCAAG
NM_003544.2         GTGTTTCTGGAGAACGTGATCCGGGACGCCGTGACCTACACGGAGCACGCCAAGCGCAAG
NM_001034077.4      GTGTTCCTGGAGAATGTGATTCGGGACGCAGTCACCTACACCGAGCACGCCAAGCGCAAG
NM_003548.2         GTGTTCCTGGAGAATGTGATTCGGGACGCAGTCACCTACACCGAGCACGCCAAGCGCAAG
NM_003542.3         GTTTTCTTAGAGAACGTTATTCGAGACGCCGTCACCTATACGGAGCACGCCAAGCGCAAA
NM_175054.2         GTCTTCCTGGAGAACGTGATCCGTGACGCGGTGACTTACACGGAGCACGCCAAGCGCAAG
NM_003540.3         GTGTTCCTGGAGAATGTGATACGGGACGCCGTAACCTACACGGAGCACGCCAAGCGTAAG
NM_003541.2         GTGTTCCTGGAGAACGTGATCCGGGACGCCGTGACCTATACAGAGCACGCCAAGCGCAAG
                    ** **  *.**.** ** ** ** ** ** ** ** ** ** **.********.** **.

HsCD00329434        ACGGTCACCGCCATGGATGTGGTCTACGCGCTCAAGCGCCAGGGCCGCACCCTCTACGGT
NM_003495.2         ACGGTCACCGCCATGGACGTGGTCTACGCGCTCAAGCGCCAGGGCCGCACCCTCTATGGC
NM_003545.3         ACAGTGACAGCGATGGATGTGGTCTACGCGCTGAAGAGACAGGGACGCACTCTTTACGGC
NM_003543.3         ACCGTGACAGCAATGGATGTGGTCTACGCGCTGAAGCGACAGGGACGCACTCTTTACGGC
NM_003539.3         ACAGTCACAGCCATGGACGTGGTGTACGCGCTCAAGCGCCAGGGACGCACTCTTTATGGC
NM_003538.3         ACAGTCACTGCCATGGACGTGGTCTACGCGCTTAAGCGCCAGGGACGCACCCTTTATGGC
NM_021968.3         ACGGTCACCGCCATGGATGTGGTCTACGCGCTCAAGCGCCAGGGCCGCACCCTCTACGGT
NM_003546.2         ACAGTCACAGCCATGGACGTGGTTTACGCGCTCAAGCGCCAGGGCCGCACCCTGTATGGC
NM_003544.2         ACTGTCACTGCCATGGATGTGGTTTACGCGCTCAAGCGTCAAGGACGCACTCTGTACGGC
NM_001034077.4      ACCGTCACAGCCATGGATGTGGTGTACGCGCTCAAGCGCCAGGGGCGCACCCTGTACGGC
NM_003548.2         ACCGTCACAGCCATGGATGTGGTGTACGCGCTCAAGCGCCAGGGGCGCACCCTGTACGGC
NM_003542.3         ACTGTCACAGCCATGGATGTAGTATATGCCCTAAAACGTCAGGGGCGCACTCTGTATGGC
NM_175054.2         ACCGTCACGGCCATGGATGTGGTGTACGCGCTGAAACGCCAGGGTCGCACCCTTTATGGT
NM_003540.3         ACAGTCACTGCAATGGATGTTGTCTACGCGCTCAAGCGCCAGGGACGCACTCTGTACGGC
NM_003541.2         ACGGTCACCGCCATGGATGTGGTCTACGCGCTCAAGCGCCAGGGCCGCACCCTCTACGGT
                    ** ** ** ** ***** ** ** ** ** ** **..* **.** ***** ** ** ** 

HsCD00329434        TTCGGTGGTTTG
NM_003495.2         TTCGGCGGCTAA
NM_003545.3         TTCGGCGGCTAA
NM_003543.3         TTCGGTGGCTAA
NM_003539.3         TTCGGCGGCTGA
NM_003538.3         TTTGGCGGTTAA
NM_021968.3         TTCGGTGGTTGA
NM_003546.2         TTTGGCGGCTGA
NM_003544.2         TTCGGCGGTTAA
NM_001034077.4      TTCGGAGGCTAG
NM_003548.2         TTCGGAGGCTAG
NM_003542.3         TTCGGCGGCTGA
NM_175054.2         TTCGGCGGTTGA
NM_003540.3         TTTGGTGGCTGA
NM_003541.2         TTCGGTGGTTGA
                    ** ** ** * .

Pathways

WikiPathway : Type II interferon signaling (IFNG)

Protein

SMART domain : H4 : Histone H4
SMART domain : TAF : TATA box binding protein associated factor
UniProt : B2R4R0
UniProt : P62805

Clone

Cloning Information : 269169
HIP Master Clone ID : 163203
Original Clone ID : FLH269169.01L

Organism


TAX_ID : 9606
Species Specific ID: 8360

Genome

Chromosome : 1
Map Location : 1q21
Ensembl : ENSG00000182217

Reagents

Labome : HIST1H4J-antibody