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LPXN (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LPXN
Gene Name:  leupaxin
Sequence             
Original Clone ID: FLH269172.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329437

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1161nts         Open reading frame : 1 to 1161
Reference Sequence Alignment

ATGGAAGAGTTAGATGCCTTATTGGAGGAACTGGAACGCTCCACCCTTCAGGACAGTGAT
GAATATTCCAACCCAGCTCCTCTTCCCCTGGATCAGCATTCCAGAAAGGAGACTAACCTT
GATGAGACTTCGGAGATCCTTTCTATTCAGGATAACACAAGTCCCTTGCCGGCGCAGCTC
GTGTATACTACCAATATCCAGGAGCTCAATGTCTACAGTGAAGCCCAAGAGCCAAAGGAA
TCACCACCACCTTCTAAAACGTCAGCAGCTGCTCAGTTGGATGAGCTCATGGCTCACCTG
ACTGAGATGCAGGCCAAGGTTGCAGTGAGAGCAGATGCTGGCAAGAAGCACTTACCAGAC
AAGCAGGATCACAAGGCCTCCCTGGACTCAATGCTTGGGGGTCTCGAGCAGGAATTGCAG
GACCTTGGCATTGCCACAGTGCCCAAGGGCCATTGTGCATCCTGCCAGAAACCGATTGCT
GGGAAGGTGATCCATGCTCTAGGGCAATCATGGCATCCTGAGCATTTTGTCTGTACTCAT
TGCAAAGAAGAGATTGGCTCCAGTCCCTTCTTTGAGCGGAGTGGCTTGGCCTACTGCCCC
AACGACTACCACCAACTTTTTTCTCCACGCTGTGCTTACTGCGCTGCTCCCATCCTGGAT
AAAGTGCTGACAGCAATGAACCAGACCTGGCACCCAGAGCACTTCTTCTGCTCTCACTGC
GGAGAGGTGTTTGGTGCAGAAGGCTTTCATGAGAAGGACAAGAAGCCATATTGCCGAAAG
GATTTCTTAGCCATGTTCTCACCCAAGTGTGGTGGCTGCAATCGCCCAGTGTTGGAAAAC
TACCTTTCAGCCATGGACACTGTCTGGCACCCAGAGTGCTTTGTTTGTGGGGACTGCTTC
ACCAGTTTTTCTACTGGCTCCTTCTTTGAACTGGATGGACGTCCATTCTGTGAGCTCCAT
TACCATCACCGCCGGGGAACGCTCTGCCATGGGTGTGGGCAGCCCATCACTGGCCGTTGT
ATCAGTGCCATGGGGTACAAGTTCCATCCTGAGCACTTTGTGTGTGCTTTCTGCCTGACA
CAGTTGTCGAAGGGCATTTTCAGGGAGCAGAATGACAAGACCTATTGTCAACCTTGCTTC
AATAAGCTCTTCCCACTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1237
Start on reference sequence 77
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LPXN
Symbol Nomenclature : LPXN
SYNONYM : LDPL
Full Nomenclature : leupaxin
GENEID : 9404
HGNC : 14061
MIM : 605390
Vega : OTTHUMG00000167466
Target GenBank: BC019035

 

Reference Sequence Alignment

  

HsCD00329437        ---------------ATGGAAGAGTTAGATGCCTTATTGGAGGAACTGGAACGCTCCACC
NM_004811.2         ---------------ATGGAAGAGTTAGATGCCTTATTGGAGGAACTGGAACGCTCCACC
NM_001143995.1      ATGTCTACATTATTGATCTCGTCTTCCGATGCCTTATTGGAGGAACTGGAACGCTCCACC
                                   **  .. . * .*********************************

HsCD00329437        CTTCAGGACAGTGATGAATATTCCAACCCAGCTCCTCTTCCCCTGGATCAGCATTCCAGA
NM_004811.2         CTTCAGGACAGTGATGAATATTCCAACCCAGCTCCTCTTCCCCTGGATCAGCATTCCAGA
NM_001143995.1      CTTCAGGACAGTGATGAATATTCCAACCCAGCTCCTCTTCCCCTGGATCAGCATTCCAGA
                    ************************************************************

HsCD00329437        AAGGAGACTAACCTTGATGAGACTTCGGAGATCCTTTCTATTCAGGATAACACAAGTCCC
NM_004811.2         AAGGAGACTAACCTTGATGAGACTTCGGAGATCCTTTCTATTCAGGATAACACAAGTCCC
NM_001143995.1      AAGGAGACTAACCTTGATGAGACTTCGGAGATCCTTTCTATTCAGGATAACACAAGTCCC
                    ************************************************************

HsCD00329437        TTGCCGGCGCAGCTCGTGTATACTACCAATATCCAGGAGCTCAATGTCTACAGTGAAGCC
NM_004811.2         TTGCCGGCGCAGCTCGTGTATACTACCAATATCCAGGAGCTCAATGTCTACAGTGAAGCC
NM_001143995.1      TTGCCGGCGCAGCTCGTGTATACTACCAATATCCAGGAGCTCAATGTCTACAGTGAAGCC
                    ************************************************************

HsCD00329437        CAAGAGCCAAAGGAATCACCACCACCTTCTAAAACGTCAGCAGCTGCTCAGTTGGATGAG
NM_004811.2         CAAGAGCCAAAGGAATCACCACCACCTTCTAAAACGTCAGCAGCTGCTCAGTTGGATGAG
NM_001143995.1      CAAGAGCCAAAGGAATCACCACCACCTTCTAAAACGTCAGCAGCTGCTCAGTTGGATGAG
                    ************************************************************

HsCD00329437        CTCATGGCTCACCTGACTGAGATGCAGGCCAAGGTTGCAGTGAGAGCAGATGCTGGCAAG
NM_004811.2         CTCATGGCTCACCTGACTGAGATGCAGGCCAAGGTTGCAGTGAGAGCAGATGCTGGCAAG
NM_001143995.1      CTCATGGCTCACCTGACTGAGATGCAGGCCAAGGTTGCAGTGAGAGCAGATGCTGGCAAG
                    ************************************************************

HsCD00329437        AAGCACTTACCAGACAAGCAGGATCACAAGGCCTCCCTGGACTCAATGCTTGGGGGTCTC
NM_004811.2         AAGCACTTACCAGACAAGCAGGATCACAAGGCCTCCCTGGACTCAATGCTTGGGGGTCTG
NM_001143995.1      AAGCACTTACCAGACAAGCAGGATCACAAGGCCTCCCTGGACTCAATGCTTGGGGGTCTG
                    *********************************************************** 

HsCD00329437        GAGCAGGAATTGCAGGACCTTGGCATTGCCACAGTGCCCAAGGGCCATTGTGCATCCTGC
NM_004811.2         GAGCAGGAATTGCAGGACCTTGGCATTGCCACAGTGCCCAAGGGCCATTGTGCATCCTGC
NM_001143995.1      GAGCAGGAATTGCAGGACCTTGGCATTGCCACAGTGCCCAAGGGCCATTGTGCATCCTGC
                    ************************************************************

HsCD00329437        CAGAAACCGATTGCTGGGAAGGTGATCCATGCTCTAGGGCAATCATGGCATCCTGAGCAT
NM_004811.2         CAGAAACCGATTGCTGGGAAGGTGATCCATGCTCTAGGGCAATCATGGCATCCTGAGCAT
NM_001143995.1      CAGAAACCGATTGCTGGGAAGGTGATCCATGCTCTAGGGCAATCATGGCATCCTGAGCAT
                    ************************************************************

HsCD00329437        TTTGTCTGTACTCATTGCAAAGAAGAGATTGGCTCCAGTCCCTTCTTTGAGCGGAGTGGC
NM_004811.2         TTTGTCTGTACTCATTGCAAAGAAGAGATTGGCTCCAGTCCCTTCTTTGAGCGGAGTGGC
NM_001143995.1      TTTGTCTGTACTCATTGCAAAGAAGAGATTGGCTCCAGTCCCTTCTTTGAGCGGAGTGGC
                    ************************************************************

HsCD00329437        TTGGCCTACTGCCCCAACGACTACCACCAACTTTTTTCTCCACGCTGTGCTTACTGCGCT
NM_004811.2         TTGGCCTACTGCCCCAACGACTACCACCAACTTTTTTCTCCACGCTGTGCTTACTGCGCT
NM_001143995.1      TTGGCCTACTGCCCCAACGACTACCACCAACTTTTTTCTCCACGCTGTGCTTACTGCGCT
                    ************************************************************

HsCD00329437        GCTCCCATCCTGGATAAAGTGCTGACAGCAATGAACCAGACCTGGCACCCAGAGCACTTC
NM_004811.2         GCTCCCATCCTGGATAAAGTGCTGACAGCAATGAACCAGACCTGGCACCCAGAGCACTTC
NM_001143995.1      GCTCCCATCCTGGATAAAGTGCTGACAGCAATGAACCAGACCTGGCACCCAGAGCACTTC
                    ************************************************************

HsCD00329437        TTCTGCTCTCACTGCGGAGAGGTGTTTGGTGCAGAAGGCTTTCATGAGAAGGACAAGAAG
NM_004811.2         TTCTGCTCTCACTGCGGAGAGGTGTTTGGTGCAGAAGGCTTTCATGAGAAGGACAAGAAG
NM_001143995.1      TTCTGCTCTCACTGCGGAGAGGTGTTTGGTGCAGAAGGCTTTCATGAGAAGGACAAGAAG
                    ************************************************************

HsCD00329437        CCATATTGCCGAAAGGATTTCTTAGCCATGTTCTCACCCAAGTGTGGTGGCTGCAATCGC
NM_004811.2         CCATATTGCCGAAAGGATTTCTTAGCCATGTTCTCACCCAAGTGTGGTGGCTGCAATCGC
NM_001143995.1      CCATATTGCCGAAAGGATTTCTTAGCCATGTTCTCACCCAAGTGTGGTGGCTGCAATCGC
                    ************************************************************

HsCD00329437        CCAGTGTTGGAAAACTACCTTTCAGCCATGGACACTGTCTGGCACCCAGAGTGCTTTGTT
NM_004811.2         CCAGTGTTGGAAAACTACCTTTCAGCCATGGACACTGTCTGGCACCCAGAGTGCTTTGTT
NM_001143995.1      CCAGTGTTGGAAAACTACCTTTCAGCCATGGACACTGTCTGGCACCCAGAGTGCTTTGTT
                    ************************************************************

HsCD00329437        TGTGGGGACTGCTTCACCAGTTTTTCTACTGGCTCCTTCTTTGAACTGGATGGACGTCCA
NM_004811.2         TGTGGGGACTGCTTCACCAGTTTTTCTACTGGCTCCTTCTTTGAACTGGATGGACGTCCA
NM_001143995.1      TGTGGGGACTGCTTCACCAGTTTTTCTACTGGCTCCTTCTTTGAACTGGATGGACGTCCA
                    ************************************************************

HsCD00329437        TTCTGTGAGCTCCATTACCATCACCGCCGGGGAACGCTCTGCCATGGGTGTGGGCAGCCC
NM_004811.2         TTCTGTGAGCTCCATTACCATCACCGCCGGGGAACGCTCTGCCATGGGTGTGGGCAGCCC
NM_001143995.1      TTCTGTGAGCTCCATTACCATCACCGCCGGGGAACGCTCTGCCATGGGTGTGGGCAGCCC
                    ************************************************************

HsCD00329437        ATCACTGGCCGTTGTATCAGTGCCATGGGGTACAAGTTCCATCCTGAGCACTTTGTGTGT
NM_004811.2         ATCACTGGCCGTTGTATCAGTGCCATGGGGTACAAGTTCCATCCTGAGCACTTTGTGTGT
NM_001143995.1      ATCACTGGCCGTTGTATCAGTGCCATGGGGTACAAGTTCCATCCTGAGCACTTTGTGTGT
                    ************************************************************

HsCD00329437        GCTTTCTGCCTGACACAGTTGTCGAAGGGCATTTTCAGGGAGCAGAATGACAAGACCTAT
NM_004811.2         GCTTTCTGCCTGACACAGTTGTCGAAGGGCATTTTCAGGGAGCAGAATGACAAGACCTAT
NM_001143995.1      GCTTTCTGCCTGACACAGTTGTCGAAGGGCATTTTCAGGGAGCAGAATGACAAGACCTAT
                    ************************************************************

HsCD00329437        TGTCAACCTTGCTTCAATAAGCTCTTCCCACTGTTG
NM_004811.2         TGTCAACCTTGCTTCAATAAGCTCTTCCCACTGTAA
NM_001143995.1      TGTCAACCTTGCTTCAATAAGCTCTTCCCACTGTAA
                    **********************************:.

Protein

SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : B4DV71
UniProt : O60711
HPRD : 05651

Clone

Cloning Information : 269172
HIP Master Clone ID : 163875
Original Clone ID : FLH269172.01L

Organism


TAX_ID : 9606
Species Specific ID: 9404

Genome

Chromosome : 11
Map Location : 11q12.1
Ensembl : ENSG00000110031

Reagents

Labome : LPXN-antibody