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FUBP1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FUBP1
Gene Name:  far upstream element (FUSE) binding protein 1
Sequence             
Original Clone ID: FLH269173.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329438

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1962nts         Open reading frame : 1 to 1962
Reference Sequence Alignment

ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGC
GGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAG
ATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTAT
GGGGGACAAAAAAGACCTTTAGAAGATGGAGATCAACCAGATGCTAAGAAAGTTGCTCCT
CAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGATCTGTAATG
ACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGAGGTGAA
CAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACAGTGGT
GGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAGCAAAA
CGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATGGCGAT
GGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTAGTCATT
GGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATGGTTATG
ATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAGGATTACAGGAGACCCA
TATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGCGGTTTC
AGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGATGTCCCC
ATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAAAAAATA
CAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCCGAAAGG
ATAGCACAAATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATTACAGAC
CTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGAGGAAGA
GGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACTACAGGAATTTAATTTT
ATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATAAAAAGC
ATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAATGCAGAT
CCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCTCGGCAA
CTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGG
CCCCATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACTCCAATG
GGACCATACAACCCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCATGGTCCT
CCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACACTGGCAGCAGCAGGCT
CCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCTTATTAC
GCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCACCAACT
ACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAGGTTGAT
TATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCTCCGACT
GGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTATAGACAA
CAAGCAGCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCAGCACCT
CAGTGCAGGTTTGATCCAGCCAGTATAGAACTAGCTCTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2036
Start on reference sequence 75
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FUBP1
Symbol Nomenclature : FUBP1
SYNONYM : FBP; FUBP
Designation : DNA helicase V|far upstream element-binding protein 1|hDH V
Full Nomenclature : far upstream element (FUSE) binding protein 1
GENEID : 8880
HGNC : 4004
MIM : 603444
Vega : OTTHUMG00000040799
Target GenBank: BC017247

 

Reference Sequence Alignment

  

HsCD00329438        ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGC
XM_005271309.1      ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGC
NM_003902.3         ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGC
                    ************************************************************

HsCD00329438        GGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAG
XM_005271309.1      GGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAG
NM_003902.3         GGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAG
                    ************************************************************

HsCD00329438        ATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTAT
XM_005271309.1      ATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTAT
NM_003902.3         ATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTAT
                    ************************************************************

HsCD00329438        GGGGGACAAAAAAGACCTTTAGAAGATG--------------------------------
XM_005271309.1      GGGGGACAAAAAAGACCTTTAGAAGATGGAGATGGCTCTTGGACAAGTCCGAGCAGTACA
NM_003902.3         GGGGGACAAAAAAGACCTTTAGAAGATG--------------------------------
                    ****************************                                

HsCD00329438        -------------------------------GAGATCAACCAGATGCTAAGAAAGTTGCT
XM_005271309.1      ACACACTGGGAGGGAATGCCCTCTCCTTTTAAAGATCAACCAGATGCTAAGAAAGTTGCT
NM_003902.3         -------------------------------GAGATCAACCAGATGCTAAGAAAGTTGCT
                                                   .****************************

HsCD00329438        CCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGAT---CT
XM_005271309.1      CCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGCAGATCT
NM_003902.3         CCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGCAGATCT
                    *****************************************************.:   **

HsCD00329438        GTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGA
XM_005271309.1      GTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGA
NM_003902.3         GTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGA
                    ************************************************************

HsCD00329438        GGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGAC
XM_005271309.1      GGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGAC
NM_003902.3         GGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGAC
                    ************************************************************

HsCD00329438        AGTGGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCA
XM_005271309.1      AGTGGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCA
NM_003902.3         AGTGGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCA
                    ************************************************************

HsCD00329438        GCAAAACGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCAT
XM_005271309.1      GCAAAACGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCAT
NM_003902.3         GCAAAACGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCAT
                    ************************************************************

HsCD00329438        GGCGATGGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTA
XM_005271309.1      GGCGATGGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTA
NM_003902.3         GGCGATGGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTA
                    ************************************************************

HsCD00329438        GTCATTGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATG
XM_005271309.1      GTCATTGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATG
NM_003902.3         GTCATTGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATG
                    ************************************************************

HsCD00329438        GTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAGGATTACAGGA
XM_005271309.1      GTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAGGATTACAGGA
NM_003902.3         GTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAGGATTACAGGA
                    ************************************************************

HsCD00329438        GACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGC
XM_005271309.1      GACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGC
NM_003902.3         GACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGC
                    ************************************************************

HsCD00329438        GGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGAT
XM_005271309.1      GGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGAT
NM_003902.3         GGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGAT
                    ************************************************************

HsCD00329438        GTCCCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAA
XM_005271309.1      GTCCCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAA
NM_003902.3         GTCCCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAA
                    ************************************************************

HsCD00329438        AAAATACAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCC
XM_005271309.1      AAAATACAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCC
NM_003902.3         AAAATACAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCC
                    ************************************************************

HsCD00329438        GAAAGGATAGCACAAATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATT
XM_005271309.1      GAAAGGATAGCACAAATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATT
NM_003902.3         GAAAGGATAGCACAAATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATT
                    ************************************************************

HsCD00329438        ACAGACCTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGA
XM_005271309.1      ACAGACCTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGA
NM_003902.3         ACAGACCTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGA
                    ************************************************************

HsCD00329438        GGAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACTACAGGAATTT
XM_005271309.1      GGAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACTACAGGAATTT
NM_003902.3         GGAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACTACAGGAATTT
                    ************************************************************

HsCD00329438        AATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATA
XM_005271309.1      AATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATA
NM_003902.3         AATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATA
                    ************************************************************

HsCD00329438        AAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAAT
XM_005271309.1      AAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAAT
NM_003902.3         AAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAAT
                    ************************************************************

HsCD00329438        GCAGATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCT
XM_005271309.1      GCAGATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCT
NM_003902.3         GCAGATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCT
                    ************************************************************

HsCD00329438        CGGCAACTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCC
XM_005271309.1      CGGCAACTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCC
NM_003902.3         CGGCAACTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCC
                    ************************************************************

HsCD00329438        CATGGGCCCCATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACT
XM_005271309.1      CATGGGCCCCATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACT
NM_003902.3         CATGGGCCCCATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACT
                    ************************************************************

HsCD00329438        CCAATGGGACCATACAACCCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCAT
XM_005271309.1      CCAATGGGACCATACAACCCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCAT
NM_003902.3         CCAATGGGACCATACAACCCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCAT
                    ************************************************************

HsCD00329438        GGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACACTGGCAGCAG
XM_005271309.1      GGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACACTGGCAGCAG
NM_003902.3         GGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACACTGGCAGCAG
                    ************************************************************

HsCD00329438        CAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCT
XM_005271309.1      CAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCT
NM_003902.3         CAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCT
                    ************************************************************

HsCD00329438        TATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCA
XM_005271309.1      TATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCA
NM_003902.3         TATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCA
                    ************************************************************

HsCD00329438        CCAACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAG
XM_005271309.1      CCAACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAG
NM_003902.3         CCAACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAG
                    ************************************************************

HsCD00329438        GTTGATTATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCT
XM_005271309.1      GTTGATTATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCT
NM_003902.3         GTTGATTATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCT
                    ************************************************************

HsCD00329438        CCGACTGGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTAT
XM_005271309.1      CCGACTGGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTAT
NM_003902.3         CCGACTGGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTAT
                    ************************************************************

HsCD00329438        AGACAACAAGCAGCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCA
XM_005271309.1      AGACAACAAGCAGCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCA
NM_003902.3         AGACAACAAGCAGCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCA
                    ************************************************************

HsCD00329438        GCACCTCAGTGCAGGTTTGATCCAGCCAGTATAGAACTAGCTCTGTTG
XM_005271309.1      GCACCTCAGGGCCAATAA------------------------------
NM_003902.3         GCACCTCAGGGCCAATAA------------------------------
                    ********* **...*::                              

Protein

SMART domain : KH : K homology RNA-binding domain
UniProt : Q96AE4
HPRD : 10349

Clone

Cloning Information : 269173
HIP Master Clone ID : 163710
Original Clone ID : FLH269173.01L

Organism


TAX_ID : 9606
Species Specific ID: 8880

Genome

Chromosome : 1
Map Location : 1p31.1
Ensembl : ENSG00000162613