DNASU Plasmid Repository • 480.965.5697 | Email

TLX3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TLX3
Gene Name:  T-cell leukemia homeobox 3
Sequence             
Original Clone ID: FLH269175.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329440

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 876nts         Open reading frame : 1 to 876
Reference Sequence Alignment

ATGGAGGCGCCCGCCAGCGCGCAGACCCCGCACCCGCACGAGCCCATCAGCTTCGGCATC
GACCAGATCCTTAACAGCCCGGACCAGGACAGCGCACCCGCCCCGCGGGGCCCCGACGGC
GCCAGCTACCTGGGAGGGCCCCCCGGGGGCCGTCCGGGCGCCACATACCCGTCTCTGCCC
GCCTCCTTTGCGGGCCTCGGCGCGCCCTTCGAGGACGCGGGATCTTACAGTGTGAACCTG
AGCCTAGCGCCCGCAGGCGTGATCCGGGTGCCGGCGCACAGGCCGCTGCCCGGGGCCGTG
CCACCGCCTCTGCCAAGCGCGCTACCCGCCATGCCCTCCGTGCCCACGGTCTCCAGCCTT
GGCGGTCTCAATTTCCCCTGGATGGAGAGCAGCCGCCGCTTCGTGAAAGACCGCTTCACA
GCGGCGGCCGCACTCACGCCCTTCACCGTGACCCGGCGCATCGGCCACCCCTACCAGAAC
CGGACGCCGCCCAAGCGTAAGAAGCCGCGCACGTCCTTTTCCCGGGTGCAGATCTGCGAG
CTGGAAAAGCGCTTCCATCGCCAGAAGTACCTGGCCTCTGCCGAGAGGGCGGCGCTCGCC
AAGTCCCTCAAAATGACGGACGCGCAGGTCAAGACCTGGTTCCAAAACCGGAGGACCAAG
TGGCGGCGGCAGACGGCGGAGGAGCGGGAGGCGGAGCGGCAGCAGGCGAGCCGGCTCATG
CTGCAGCTGCAACACGACGCCTTCCAAAAGAGCCTCAACGACTCCATCCAGCCTGACCCG
CTCTGTCTGCACAACTCGTCACTCTTTGCTCTGCAGAATCTGCAGCCCTGGGAGGAGGAT
AGTTCCAAGGTTCCCGCTGTCACCTCCCTGGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 967
Start on reference sequence 92
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TLX3
Symbol Nomenclature : TLX3
SYNONYM : HOX11L2; RNX
Designation : T-cell leukemia homeobox protein 3|T-cell leukemia, homeobox 3|homeo box 11-like 2|homeobox protein Hox-11L2
Full Nomenclature : T-cell leukemia homeobox 3
GENEID : 30012
HGNC : 13532
MIM : 604640
Vega : OTTHUMG00000163207
Target GenBank: BC017291

 

Reference Sequence Alignment

  

HsCD00329440      ATGGAGGCGCCCGCCAGCGCGCAGACCCCGCACCCGCACGAGCCCATCAGCTTCGGCATC
NM_021025.2       ATGGAGGCGCCCGCCAGCGCGCAGACCCCGCACCCGCACGAGCCCATCAGCTTCGGCATC
                  ************************************************************

HsCD00329440      GACCAGATCCTTAACAGCCCGGACCAGGACAGCGCACCCGCCCCGCGGGGCCCCGACGGC
NM_021025.2       GACCAGATCCTTAACAGCCCGGACCAGGACAGCGCACCCGCCCCGCGGGGCCCCGACGGC
                  ************************************************************

HsCD00329440      GCCAGCTACCTGGGAGGGCCCCCCGGGGGCCGTCCGGGCGCCACATACCCGTCTCTGCCC
NM_021025.2       GCCAGCTACCTGGGAGGGCCCCCCGGGGGCCGTCCGGGCGCCACATACCCGTCTCTGCCC
                  ************************************************************

HsCD00329440      GCCTCCTTTGCGGGCCTCGGCGCGCCCTTCGAGGACGCGGGATCTTACAGTGTGAACCTG
NM_021025.2       GCCTCCTTTGCGGGCCTCGGCGCGCCCTTCGAGGACGCGGGATCTTACAGTGTGAACCTG
                  ************************************************************

HsCD00329440      AGCCTAGCGCCCGCAGGCGTGATCCGGGTGCCGGCGCACAGGCCGCTGCCCGGGGCCGTG
NM_021025.2       AGCCTAGCGCCCGCAGGCGTGATCCGGGTGCCGGCGCACAGGCCGCTGCCCGGGGCCGTG
                  ************************************************************

HsCD00329440      CCACCGCCTCTGCCAAGCGCGCTACCCGCCATGCCCTCCGTGCCCACGGTCTCCAGCCTT
NM_021025.2       CCACCGCCTCTGCCAAGCGCGCTACCCGCCATGCCCTCCGTGCCCACGGTCTCCAGCCTT
                  ************************************************************

HsCD00329440      GGCGGTCTCAATTTCCCCTGGATGGAGAGCAGCCGCCGCTTCGTGAAAGACCGCTTCACA
NM_021025.2       GGCGGTCTCAATTTCCCCTGGATGGAGAGCAGCCGCCGCTTCGTGAAAGACCGCTTCACA
                  ************************************************************

HsCD00329440      GCGGCGGCCGCACTCACGCCCTTCACCGTGACCCGGCGCATCGGCCACCCCTACCAGAAC
NM_021025.2       GCGGCGGCCGCACTCACGCCCTTCACCGTGACCCGGCGCATCGGCCACCCCTACCAGAAC
                  ************************************************************

HsCD00329440      CGGACGCCGCCCAAGCGTAAGAAGCCGCGCACGTCCTTTTCCCGGGTGCAGATCTGCGAG
NM_021025.2       CGGACGCCGCCCAAGCGTAAGAAGCCGCGCACGTCCTTTTCCCGGGTGCAGATCTGCGAG
                  ************************************************************

HsCD00329440      CTGGAAAAGCGCTTCCATCGCCAGAAGTACCTGGCCTCTGCCGAGAGGGCGGCGCTCGCC
NM_021025.2       CTGGAAAAGCGCTTCCATCGCCAGAAGTACCTGGCCTCTGCCGAGAGGGCGGCGCTCGCC
                  ************************************************************

HsCD00329440      AAGTCCCTCAAAATGACGGACGCGCAGGTCAAGACCTGGTTCCAAAACCGGAGGACCAAG
NM_021025.2       AAGTCCCTCAAAATGACGGACGCGCAGGTCAAGACCTGGTTCCAAAACCGGAGGACCAAG
                  ************************************************************

HsCD00329440      TGGCGGCGGCAGACGGCGGAGGAGCGGGAGGCGGAGCGGCAGCAGGCGAGCCGGCTCATG
NM_021025.2       TGGCGGCGGCAGACGGCGGAGGAGCGGGAGGCGGAGCGGCAGCAGGCGAGCCGGCTCATG
                  ************************************************************

HsCD00329440      CTGCAGCTGCAACACGACGCCTTCCAAAAGAGCCTCAACGACTCCATCCAGCCTGACCCG
NM_021025.2       CTGCAGCTGCAACACGACGCCTTCCAAAAGAGCCTCAACGACTCCATCCAGCCTGACCCG
                  ************************************************************

HsCD00329440      CTCTGTCTGCACAACTCGTCACTCTTTGCTCTGCAGAATCTGCAGCCCTGGGAGGAGGAT
NM_021025.2       CTCTGTCTGCACAACTCGTCACTCTTTGCTCTGCAGAATCTGCAGCCCTGGGAGGAGGAT
                  ************************************************************

HsCD00329440      AGTTCCAAGGTTCCCGCTGTCACCTCCCTGGTGTTG
NM_021025.2       AGTTCCAAGGTTCCCGCTGTCACCTCCCTGGTGTGA
                  ********************************** .

Pathways

WikiPathway : Cell Differentiation - Index
WikiPathway : Cell Differentiation - meta
WikiPathway : SIDS Susceptibility Pathways

Protein

SMART domain : HOX : Homeodomain
UniProt : O43711
HPRD : 06868

Clone

Cloning Information : 269175
HIP Master Clone ID : 163626
Original Clone ID : FLH269175.01L

Organism


TAX_ID : 9606
Species Specific ID: 30012

Genome

Chromosome : 5
Map Location : 5q35.1
Ensembl : ENSG00000164438

Reagents

Labome : HOX11L2-antibody