DNASU Plasmid Repository • 480.965.5697 | Email

POLR2I (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  POLR2I
Gene Name:  polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Sequence             
Original Clone ID: FLH269181.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329446

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 378nts         Open reading frame : 1 to 378
Reference Sequence Alignment

ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGT
AACAACATGCTGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGG
AACTGTGATTACCAGCAGGAGGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCAC
GAAGTGGACGAACTGACCCAGATTATCGCCGACGTGTCCCAGGACCCCACGTTGCCGCGG
ACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGGCTGTGTTCTTCCAGTCACAC
AGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCCACACTGCGGC
CACCGCTGGACCGAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 688
Start on reference sequence 311
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POLR2I
Symbol Nomenclature : POLR2I
SYNONYM : RPB9; hRPB14.5
Designation : DNA-directed RNA polymerase II 14.5 kDa polypeptide|DNA-directed RNA polymerase II subunit I|DNA-directed RNA polymerase II subunit RPB9|RNA polymerase II 14.5 kDa subunit|RNA polymerase II subunit B9|RPB14.5
Full Nomenclature : polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
GENEID : 5438
HGNC : 9196
MIM : 180662
Vega : OTTHUMG00000181749
Target GenBank: BC017112

 

Reference Sequence Alignment

  

HsCD00329446      ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGT
NM_006233.4       ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGT
                  ************************************************************

HsCD00329446      AACAACATGCTGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGG
NM_006233.4       AACAACATGCTGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGG
                  ************************************************************

HsCD00329446      AACTGTGATTACCAGCAGGAGGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCAC
NM_006233.4       AACTGTGATTACCAGCAGGAGGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCAC
                  ************************************************************

HsCD00329446      GAAGTGGACGAACTGACCCAGATTATCGCCGACGTGTCCCAGGACCCCACGTTGCCGCGG
NM_006233.4       GAAGTGGACGAACTGACCCAGATTATCGCCGACGTGTCCCAGGACCCCACGTTGCCGCGG
                  ************************************************************

HsCD00329446      ACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGGCTGTGTTCTTCCAGTCACAC
NM_006233.4       ACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGGCTGTGTTCTTCCAGTCACAC
                  ************************************************************

HsCD00329446      AGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCCACACTGCGGC
NM_006233.4       AGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCCACACTGCGGC
                  ************************************************************

HsCD00329446      CACCGCTGGACCGAGTTG
NM_006233.4       CACCGCTGGACCGAGTGA
                  **************** .

Pathways

Reactome : Abortive elongation of HIV-1 transcript in the absence of Tat
Reactome : DNA Repair
Reactome : Disease
Reactome : Dual incision reaction in TC-NER
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : HIV elongation arrest and recovery
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : Late Phase of HIV Life Cycle
Reactome : MicroRNA (miRNA) biogenesis
Reactome : Nucleotide Excision Repair
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Regulatory RNA pathways
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)
Reactome : Viral Messenger RNA Synthesis
Reactome : mRNA Capping
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
Reactome : mRNA Splicing - Minor Pathway
WikiPathway : Eukaryotic Transcription Initiation

Protein

SMART domain : ZnF_C2C2 : C2C2 Zinc finger
SMART domain : RPOL9 : RNA polymerase subunit 9
UniProt : P36954
HPRD : 15944

Clone

Cloning Information : 269181
HIP Master Clone ID : 170013
Original Clone ID : FLH269181.01L

Organism


TAX_ID : 9606
Species Specific ID: 5438

Genome

Chromosome : 19
Map Location : 19q12
Ensembl : ENSG00000105258

Reagents

Labome : POLR2I-antibody