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ZNF461 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF461
Gene Name:  zinc finger protein 461
Sequence             
Original Clone ID: FLH269193.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329458

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1311nts         Open reading frame : 1 to 1311
Reference Sequence Alignment

ATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATTTTCAGCGCTATCTGGGAAGGC
AACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGTTATTTTAGACAACTTATGATT
AACCATGAAAACATGCCCATTTTTAGCCAACATACTTTACTCACTCAAGAATTTTATGAT
AGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATCTTCAGTTACCATTTATTTTTT
AGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAATGTAAAGAATGCACAGAAATT
GTTAATACACCATGCCTTTTTAAACAACAGACAATTCAAAATGGTGACAAATGCAATGAG
TGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAACTTAAACATCTAAGAATTCAT
AATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAGGCCTTTAATTATGGCTCAGAA
CTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCTTATGAATGTAAAGAATGTGGG
AAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAGAGACTTCATACTGGTGAAAAA
CCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGTGGCTTTCAACTTACTGAACAC
CTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAAGAATGTGGAAAGACTTTTAGG
CATCGCTCACATCTTACTATACATCAGAGAATTCATACTGGTGAGAAACCCTATGAATGT
CGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTCTCACACCATCAGAAAATTCAT
TCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAGGCTTTTTGTGATGGCTTACAA
CTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCCTATGAGTGTAAGGAATGTGGG
AAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAGAGAATTCATACTGGTGAGAAA
CCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTTCATTCACACCTTATTCAACAT
CAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAGGAATGTGGGAAGGCCTTTAGC
TATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCTGGAAAGAAACCTTATCAATGC
GGGAAGGCGTTTAATCATAGATTACAACTTAACTTACATCAGACTCTTCATACTGGCGAG
AAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGTCTAGCATCATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1730
Start on reference sequence 420
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF461
Symbol Nomenclature : ZNF461
SYNONYM : GIOT-1; GIOT1
Designation : gonadotropin inducible transcription repressor 1|gonadotropin-inducible ovary transcription repressor 1
Full Nomenclature : zinc finger protein 461
GENEID : 92283
HGNC : 21629
MIM : 608640
Vega : OTTHUMG00000180832
Target GenBank: BC028631

 

Reference Sequence Alignment

  

HsCD00329458        ------------------------------------------------------------
NM_153257.2         ATGGCCCATGAGTTGGTGATGTTCAGAGATGTGGCTATAGATGTCTCTCAGGAGGAATGG
XM_005259402.1      ATGGCCCATGAGTTGGTGATGTTCAGAGATGTGGCTATAGATGTCTCTCAGGAGGAATGG
XM_005259403.1      --------------------------------------------------------ATGG
                                                                                

HsCD00329458        ------------------------------------------------------------
NM_153257.2         GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAGGTGATGTTGGAGAATTATAGC
XM_005259402.1      GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAGGTGATGTTGGAGAATTATAGC
XM_005259403.1      GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAG---------------------
                                                                                

HsCD00329458        ------------------------------------------------------------
NM_153257.2         AACTTGGTGTCACTTGGACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
XM_005259402.1      AACTTGGTGTCACTTGGACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
XM_005259403.1      ----------------GACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
                                                                                

HsCD00329458        ------------------------------------------------------------
NM_153257.2         GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAGGTACATGG
XM_005259402.1      GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAG--------
XM_005259403.1      GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAGGTACATGG
                                                                                

HsCD00329458        ------------------------------------------------------------
NM_153257.2         AAAACTTGGGGATTTCACAATAATTTCTTGGACAATAATGAGGCTACAGACATAAATGCA
XM_005259402.1      ------------------------------------------------------------
XM_005259403.1      AAAACTTGGGGATTTCACAATAATTTCTTGGACAATAATGAGGCTACAGACATAAATGCA
                                                                                

HsCD00329458        ------------------------------------------------------------
NM_153257.2         GACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
XM_005259402.1      -ACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
XM_005259403.1      GACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
                                                                                

HsCD00329458        ---------------------ATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
NM_153257.2         GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
XM_005259402.1      GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
XM_005259403.1      GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
                                         ***************************************

HsCD00329458        TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
NM_153257.2         TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
XM_005259402.1      TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
XM_005259403.1      TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
                    ************************************************************

HsCD00329458        TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
NM_153257.2         TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
XM_005259402.1      TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
XM_005259403.1      TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
                    ************************************************************

HsCD00329458        CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
NM_153257.2         CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
XM_005259402.1      CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
XM_005259403.1      CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
                    ************************************************************

HsCD00329458        TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
NM_153257.2         TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
XM_005259402.1      TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
XM_005259403.1      TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
                    ************************************************************

HsCD00329458        TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
NM_153257.2         TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
XM_005259402.1      TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
XM_005259403.1      TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
                    ************************************************************

HsCD00329458        AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
NM_153257.2         AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
XM_005259402.1      AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
XM_005259403.1      AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
                    ************************************************************

HsCD00329458        CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
NM_153257.2         CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
XM_005259402.1      CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
XM_005259403.1      CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
                    ************************************************************

HsCD00329458        GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
NM_153257.2         GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
XM_005259402.1      GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
XM_005259403.1      GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
                    ************************************************************

HsCD00329458        TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
NM_153257.2         TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
XM_005259402.1      TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
XM_005259403.1      TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
                    ************************************************************

HsCD00329458        AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
NM_153257.2         AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
XM_005259402.1      AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
XM_005259403.1      AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
                    ************************************************************

HsCD00329458        GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
NM_153257.2         GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
XM_005259402.1      GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
XM_005259403.1      GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
                    ************************************************************

HsCD00329458        GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
NM_153257.2         GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
XM_005259402.1      GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
XM_005259403.1      GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
                    ************************************************************

HsCD00329458        GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
NM_153257.2         GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
XM_005259402.1      GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
XM_005259403.1      GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
                    ************************************************************

HsCD00329458        TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
NM_153257.2         TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
XM_005259402.1      TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
XM_005259403.1      TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
                    ************************************************************

HsCD00329458        GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
NM_153257.2         GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
XM_005259402.1      GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
XM_005259403.1      GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
                    ************************************************************

HsCD00329458        TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
NM_153257.2         TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
XM_005259402.1      TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
XM_005259403.1      TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
                    ************************************************************

HsCD00329458        AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
NM_153257.2         AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
XM_005259402.1      AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
XM_005259403.1      AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
                    ************************************************************

HsCD00329458        CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
NM_153257.2         CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
XM_005259402.1      CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
XM_005259403.1      CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
                    ************************************************************

HsCD00329458        GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
NM_153257.2         GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
XM_005259402.1      GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
XM_005259403.1      GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
                    ************************************************************

HsCD00329458        GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
NM_153257.2         GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
XM_005259402.1      GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
XM_005259403.1      GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
                    ************************************************************

HsCD00329458        CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
NM_153257.2         CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
XM_005259402.1      CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
XM_005259403.1      CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
                    ************************************************************

HsCD00329458        CTAGCATCATTG
NM_153257.2         CTAGCATCATGA
XM_005259402.1      CTAGCATCATGA
XM_005259403.1      CTAGCATCATGA
                    ********** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8TAF7
HPRD : 16358

Clone

Cloning Information : 269193
HIP Master Clone ID : 143423
Original Clone ID : FLH269193.01L

Organism


TAX_ID : 9606
Species Specific ID: 92283

Genome

Chromosome : 19
Map Location : 19q13.12
Ensembl : ENSG00000197808

Reagents

Labome : ZNF461-antibody