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PDLIM1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PDLIM1
Gene Name:  PDZ and LIM domain 1
Sequence             
Original Clone ID: FLH268347.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329460

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 990nts         Open reading frame : 1 to 990
Reference Sequence Alignment

ATGACCACCCAGCAGATAGACCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGC
GGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCT
CTAGCTAATTTATGTATTGGAGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAAT
ATGACACACTTGGAAGCTCAGAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACT
GTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCAT
CCATACAAGATGAATTTAGCCTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCAC
AACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATC
ACAAACCAGTACAACAACCCAGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAAC
AATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGACCCTTAGAC
CATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTT
CAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACAGTCCACGTCTTTCTTGGTTTTGCAG
GAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAACAAGCCCTCAGGATTCAGAAGTGTT
AAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAGAAGTTGCCTATGTGT
GACAAATGTGGCACTGGGATTGTTGGTGTGTTTGTGAAGCTGCGGGACCGTCACCGCCAC
CCTGAGTGTTATGTGTGCACTGACTGTGGCACCAACCTGAAACAGAAGGGCCATTTCTTT
GTGGAGGATCAAATCTACTGTGAGAAGCATGCCCGGGAGCGAGTCACACCACCTGAGGGT
TATGAAGTGGTCACTGTGTTCCCCAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1014
Start on reference sequence 25
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PDLIM1
Symbol Nomenclature : PDLIM1
SYNONYM : CLIM1; CLP-36; CLP36; HEL-S-112; hCLIM1
Designation : LIM domain protein CLP-36|PDZ and LIM domain protein 1|carboxyl terminal LIM domain protein 1|elfin|epididymis secretory protein Li 112
Full Nomenclature : PDZ and LIM domain 1
GENEID : 9124
HGNC : 2067
MIM : 605900
Vega : OTTHUMG00000018810
Target GenBank: BC018755

 

Reference Sequence Alignment

  

HsCD00329460      ATGACCACCCAGCAGATAGACCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGC
NM_020992.3       ATGACCACCCAGCAGATAGACCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGC
                  ************************************************************

HsCD00329460      GGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCT
NM_020992.3       GGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCT
                  ************************************************************

HsCD00329460      CTAGCTAATTTATGTATTGGAGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAAT
NM_020992.3       CTAGCTAATTTATGTATTGGAGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAAT
                  ************************************************************

HsCD00329460      ATGACACACTTGGAAGCTCAGAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACT
NM_020992.3       ATGACACACTTGGAAGCTCAGAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACT
                  ************************************************************

HsCD00329460      GTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCAT
NM_020992.3       GTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCAT
                  ************************************************************

HsCD00329460      CCATACAAGATGAATTTAGCCTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCAC
NM_020992.3       CCATACAAGATGAATTTAGCCTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCAC
                  ************************************************************

HsCD00329460      AACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATC
NM_020992.3       AACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATC
                  ************************************************************

HsCD00329460      ACAAACCAGTACAACAACCCAGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAAC
NM_020992.3       ACAAACCAGTACAACAACCCAGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAAC
                  ************************************************************

HsCD00329460      AATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGACCCTTAGAC
NM_020992.3       AATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGACCCTTAGAC
                  ************************************************************

HsCD00329460      CATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTT
NM_020992.3       CATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTT
                  ************************************************************

HsCD00329460      CAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACAGTCCACGTCTTTCTTGGTTTTGCAG
NM_020992.3       CAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACAGTCCACGTCTTTCTTGGTTTTGCAG
                  ************************************************************

HsCD00329460      GAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAACAAGCCCTCAGGATTCAGAAGTGTT
NM_020992.3       GAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAACAAGCCCTCAGGATTCAGAAGTGTT
                  ************************************************************

HsCD00329460      AAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAGAAGTTGCCTATGTGT
NM_020992.3       AAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAGAAGTTGCCTATGTGT
                  ************************************************************

HsCD00329460      GACAAATGTGGCACTGGGATTGTTGGTGTGTTTGTGAAGCTGCGGGACCGTCACCGCCAC
NM_020992.3       GACAAATGTGGCACTGGGATTGTTGGTGTGTTTGTGAAGCTGCGGGACCGTCACCGCCAC
                  ************************************************************

HsCD00329460      CCTGAGTGTTATGTGTGCACTGACTGTGGCACCAACCTGAAACAGAAGGGCCATTTCTTT
NM_020992.3       CCTGAGTGTTATGTGTGCACTGACTGTGGCACCAACCTGAAACAGAAGGGCCATTTCTTT
                  ************************************************************

HsCD00329460      GTGGAGGATCAAATCTACTGTGAGAAGCATGCCCGGGAGCGAGTCACACCACCTGAGGGT
NM_020992.3       GTGGAGGATCAAATCTACTGTGAGAAGCATGCCCGGGAGCGAGTCACACCACCTGAGGGT
                  ************************************************************

HsCD00329460      TATGAAGTGGTCACTGTGTTCCCCAAGTTG
NM_020992.3       TATGAAGTGGTCACTGTGTTCCCCAAGTGA
                  **************************** .

Clone

Cloning Information : 268347
HIP Master Clone ID : 193205
Original Clone ID : FLH268347.01L

Organism


TAX_ID : 9606
Species Specific ID: 9124

Genome

Chromosome : 10
Map Location : 10q23.1
Ensembl : ENSG00000107438

Reagents

Labome : PDLIM1-antibody