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ZNF354A (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF354A
Gene Name:  zinc finger protein 354A
Sequence             
Original Clone ID: FLH268350.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329463

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1818nts         Open reading frame : 1 to 1818
Reference Sequence Alignment

ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACGTTTGAGGATGTGGCT
GTGCTGTTTACCCGAGATGAGTGGAGAAAGCTGGCCCCTTCTCAGAGAAACTTGTACCGG
GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGGCTCCCATTTACCAAACCA
AAAGTGATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACGGTTCT
GGCGTCTCCTCTCTAGGATCGAAGAGCAGTCATAAAACCACAAAGTCAACGCAAACACAA
GACTCTTCATTTCAGGGACTGATACTGAAAAGATCCAACAGGAATGTACCTTGGGATTTG
AAATTAGAAAAGCCTTACATATATGAAGGCAGATTAGAGAAAAAGCAGGATAAAAAGGGA
AGTTTTCAGATAGTTTCAGCCACCCACAAAAAAATCCCCACTATAGAAAGAAGCCATAAA
AATACTGAATTGAGCCAAAACTTCAGCCCAAAGTCAGTGCTTATTAGGCAACAGATACTT
CCCAGAGAAAAAACACCACCAAAATGTGAAATACAAGGAAACAGCCTCAAACAGAATTCA
CAATTACTTAATCAACCAAAAATTACAGCAGATAAACGCTATAAATGTAGTCTGTGTGAA
AAAACCTTCATTAACACTTCATCCCTTCGTAAACATGAGAAAAACCATAGTGGAGAGAAA
CTATTTAAGTGTAAAGAATGTTCAAAAGCCTTTAGCCAAAGTTCAGCCCTTATTCAACAT
CAAATAACGCATACTGGAGAGAAACCCTACATATGTAAAGAATGTGGGAAAGCCTTTACT
CTCAGTACATCCCTTTATAAACATCTAAGAACCCATACTGTGGAGAAATCCTACAGCTGT
AAAGAATGTGGTAAATCCTTCAGCCGAAGGTCAGGCCTTTTTATACATCAAAAAATTCAT
GCTGAAGAAAACCCTTGTAAGTATAATCCGGGTAGGAAGGCATCTAGTTGCAGCACATCC
CTTTCTGGATGTCAAAGAATTCATTCTAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
TCTATTTCACGACTTAATAGACACCGAATCATTCATACTGGAGAGAAGTTTTATAATTGT
AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCACGAGCGAATTCAT
ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCAGCT
CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAATGTAACGAGTGTGGG
AAAACATTCAGGTGTAACTCATCACTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
CCATATCGATGTGAGGAATGTGGGATATCTTTTGGCCAAAGTTCAGCTCTTATTCAGCAT
CGAAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
CAAAGCTCATCACGTATTGCACATCAGAGAATTCATACTGGAGAGAAACCCTATGAATGT
AATACATGTGGGAAACTTTTCAACCATAGGTCATCCCTTACTAATCATTATAAAATTCAT
ATCGAAGAGGACCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1973
Start on reference sequence 156
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF354A
Symbol Nomenclature : ZNF354A
SYNONYM : EZNF; HEL104; HKL1; KID-1; KID1; TCF17
Designation : TCF-17|epididymis luminal protein 104|transcription factor 17|zinc finger protein eZNF
Full Nomenclature : zinc finger protein 354A
GENEID : 6940
HGNC : 11628
MIM : 602444
Vega : OTTHUMG00000130895
Target GenBank: BC047105

 

Reference Sequence Alignment

  

HsCD00329463      ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACGTTTGAGGATGTGGCT
NM_005649.2       ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACGTTTGAGGATGTGGCT
                  ************************************************************

HsCD00329463      GTGCTGTTTACCCGAGATGAGTGGAGAAAGCTGGCCCCTTCTCAGAGAAACTTGTACCGG
NM_005649.2       GTGCTGTTTACCCGAGATGAGTGGAGAAAGCTGGCCCCTTCTCAGAGAAACTTGTACCGG
                  ************************************************************

HsCD00329463      GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGGCTCCCATTTACCAAACCA
NM_005649.2       GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGGCTCCCATTTACCAAACCA
                  ************************************************************

HsCD00329463      AAAGTGATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACGGTTCT
NM_005649.2       AAAGTGATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACGGTTCT
                  ************************************************************

HsCD00329463      GGCGTCTCCTCTCTAGGATCGAAGAGCAGTCATAAAACCACAAAGTCAACGCAAACACAA
NM_005649.2       GGCGTCTCCTCTCTAGGATCGAAGAGCAGTCATAAAACCACAAAGTCAACGCAAACACAA
                  ************************************************************

HsCD00329463      GACTCTTCATTTCAGGGACTGATACTGAAAAGATCCAACAGGAATGTACCTTGGGATTTG
NM_005649.2       GACTCTTCATTTCAGGGACTGATACTGAAAAGATCCAACAGGAATGTACCTTGGGATTTG
                  ************************************************************

HsCD00329463      AAATTAGAAAAGCCTTACATATATGAAGGCAGATTAGAGAAAAAGCAGGATAAAAAGGGA
NM_005649.2       AAATTAGAAAAGCCTTACATATATGAAGGCAGATTAGAGAAAAAGCAGGATAAAAAGGGA
                  ************************************************************

HsCD00329463      AGTTTTCAGATAGTTTCAGCCACCCACAAAAAAATCCCCACTATAGAAAGAAGCCATAAA
NM_005649.2       AGTTTTCAGATAGTTTCAGCCACCCACAAAAAAATCCCCACTATAGAAAGAAGCCATAAA
                  ************************************************************

HsCD00329463      AATACTGAATTGAGCCAAAACTTCAGCCCAAAGTCAGTGCTTATTAGGCAACAGATACTT
NM_005649.2       AATACTGAATTGAGCCAAAACTTCAGCCCAAAGTCAGTGCTTATTAGGCAACAGATACTT
                  ************************************************************

HsCD00329463      CCCAGAGAAAAAACACCACCAAAATGTGAAATACAAGGAAACAGCCTCAAACAGAATTCA
NM_005649.2       CCCAGAGAAAAAACACCACCAAAATGTGAAATACAAGGAAACAGCCTCAAACAGAATTCA
                  ************************************************************

HsCD00329463      CAATTACTTAATCAACCAAAAATTACAGCAGATAAACGCTATAAATGTAGTCTGTGTGAA
NM_005649.2       CAATTACTTAATCAACCAAAAATTACAGCAGATAAACGCTATAAATGTAGTCTGTGTGAA
                  ************************************************************

HsCD00329463      AAAACCTTCATTAACACTTCATCCCTTCGTAAACATGAGAAAAACCATAGTGGAGAGAAA
NM_005649.2       AAAACCTTCATTAACACTTCATCCCTTCGTAAACATGAGAAAAACCATAGTGGAGAGAAA
                  ************************************************************

HsCD00329463      CTATTTAAGTGTAAAGAATGTTCAAAAGCCTTTAGCCAAAGTTCAGCCCTTATTCAACAT
NM_005649.2       CTATTTAAGTGTAAAGAATGTTCAAAAGCCTTTAGCCAAAGTTCAGCTCTTATTCAACAT
                  *********************************************** ************

HsCD00329463      CAAATAACGCATACTGGAGAGAAACCCTACATATGTAAAGAATGTGGGAAAGCCTTTACT
NM_005649.2       CAAATAACGCATACTGGAGAGAAACCCTACATATGTAAAGAATGTGGGAAAGCCTTTACT
                  ************************************************************

HsCD00329463      CTCAGTACATCCCTTTATAAACATCTAAGAACCCATACTGTGGAGAAATCCTACAGCTGT
NM_005649.2       CTCAGTACATCCCTTTATAAACATCTAAGAACCCATACTGTGGAGAAATCCTACAGATGT
                  ********************************************************.***

HsCD00329463      AAAGAATGTGGTAAATCCTTCAGCCGAAGGTCAGGCCTTTTTATACATCAAAAAATTCAT
NM_005649.2       AAAGAATGTGGTAAATCCTTCAGCCGAAGGTCAGGCCTTTTTATACATCAAAAAATTCAT
                  ************************************************************

HsCD00329463      GCTGAAGAAAACCCTTGTAAGTATAATCCGGGTAGGAAGGCATCTAGTTGCAGCACATCC
NM_005649.2       GCTGAAGAAAACCCTTGTAAGTATAATCCGGGTAGGAAGGCATCTAGTTGCAGCACATCC
                  ************************************************************

HsCD00329463      CTTTCTGGATGTCAAAGAATTCATTCTAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
NM_005649.2       CTTTCTGGATGTCAAAGAATTCATTCTAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
                  ************************************************************

HsCD00329463      AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
NM_005649.2       AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
                  ************************************************************

HsCD00329463      CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
NM_005649.2       CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
                  ************************************************************

HsCD00329463      GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
NM_005649.2       GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
                  ************************************************************

HsCD00329463      TCTATTTCACGACTTAATAGACACCGAATCATTCATACTGGAGAGAAGTTTTATAATTGT
NM_005649.2       TCTATTTCACGACTTAATAGACACCGAATCATTCATACTGGAGAGAAGTTTTATAATTGT
                  ************************************************************

HsCD00329463      AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCACGAGCGAATTCAT
NM_005649.2       AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCACGAGCGAATTCAT
                  ************************************************************

HsCD00329463      ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCAGCT
NM_005649.2       ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCAGCT
                  ************************************************************

HsCD00329463      CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAATGTAACGAGTGTGGG
NM_005649.2       CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAATGTAACGAGTGTGGG
                  ************************************************************

HsCD00329463      AAAACATTCAGGTGTAACTCATCACTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
NM_005649.2       AAAACATTCAGGTGTAACTCATCACTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
                  ************************************************************

HsCD00329463      CCATATCGATGTGAGGAATGTGGGATATCTTTTGGCCAAAGTTCAGCTCTTATTCAGCAT
NM_005649.2       CCATATCGATGTGAGGAATGTGGGATATCTTTTGGCCAAAGTTCAGCTCTTATTCAGCAT
                  ************************************************************

HsCD00329463      CGAAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
NM_005649.2       CGAAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
                  ************************************************************

HsCD00329463      CAAAGCTCATCACGTATTGCACATCAGAGAATTCATACTGGAGAGAAACCCTATGAATGT
NM_005649.2       CAAAGCTCATCACGTATTGCACATCAGAGAATTCATACTGGAGAGAAACCCTATGAATGT
                  ************************************************************

HsCD00329463      AATACATGTGGGAAACTTTTCAACCATAGGTCATCCCTTACTAATCATTATAAAATTCAT
NM_005649.2       AATACATGTGGGAAACTTTTCAACCATAGGTCATCCCTTACTAATCATTATAAAATTCAT
                  ************************************************************

HsCD00329463      ATCGAAGAGGACCCCTTG
NM_005649.2       ATCGAAGAGGACCCCTAG
                  ****************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : O60765
HPRD : 03900

Clone

Cloning Information : 268350
HIP Master Clone ID : 193208
Original Clone ID : FLH268350.01L

Organism


TAX_ID : 9606
Species Specific ID: 6940

Genome

Chromosome : 5
Map Location : 5q35.3
Ensembl : ENSG00000169131

Reagents

Labome : ZNF354A-antibody