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KLF8 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLF8
Gene Name:  Kruppel-like factor 8
Sequence             
Original Clone ID: FLH268351.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329464

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1080nts         Open reading frame : 1 to 1080
Reference Sequence Alignment

ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1368
Start on reference sequence 289
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLF8
Symbol Nomenclature : KLF8
SYNONYM : BKLF3; ZNF741
Designation : Krueppel-like factor 8|basic krueppel-like factor 3|basic kruppel-like factor 3|zinc finger protein 741
Full Nomenclature : Kruppel-like factor 8
GENEID : 11279
Locus Tag : RP13-1021K9.1
HGNC : 6351
MIM : 300286
Target GenBank: BC112109

 

Reference Sequence Alignment

  

HsCD00329464        ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
XM_005261977.1      ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
XM_005261979.1      ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
XM_005261978.1      ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
NM_007250.4         ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGC
                    ************************************************************

HsCD00329464        TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
XM_005261977.1      TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
XM_005261979.1      TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
XM_005261978.1      TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
NM_007250.4         TCAATGCAGGTATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATA
                    ************************************************************

HsCD00329464        GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
XM_005261977.1      GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
XM_005261979.1      GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
XM_005261978.1      GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
NM_007250.4         GAATACAGAAGTAATATGACTTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCA
                    ************************************************************

HsCD00329464        GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
XM_005261977.1      GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
XM_005261979.1      GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
XM_005261978.1      GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
NM_007250.4         GCACTGTTTAATGACATCAAGATTGAGCCCCCAGAAGAACTTTTGGCTAGTGATTTCAGC
                    ************************************************************

HsCD00329464        CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
XM_005261977.1      CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
XM_005261979.1      CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
XM_005261978.1      CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
NM_007250.4         CTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGCCCAAGGCTCCTCTCCAGCCT
                    ************************************************************

HsCD00329464        GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
XM_005261977.1      GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
XM_005261979.1      GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
XM_005261978.1      GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
NM_007250.4         GCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCCCCAGACCCTT
                    ************************************************************

HsCD00329464        GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
XM_005261977.1      GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
XM_005261979.1      GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
XM_005261978.1      GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
NM_007250.4         GTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC
                    ************************************************************

HsCD00329464        CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
XM_005261977.1      CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
XM_005261979.1      CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
XM_005261978.1      CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
NM_007250.4         CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATT
                    ************************************************************

HsCD00329464        CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
XM_005261977.1      CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
XM_005261979.1      CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
XM_005261978.1      CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
NM_007250.4         CACACTATCCCCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTG
                    ************************************************************

HsCD00329464        GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
XM_005261977.1      GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
XM_005261979.1      GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
XM_005261978.1      GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
NM_007250.4         GTAGTGCAGTCTCTGCCCATGGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCC
                    ************************************************************

HsCD00329464        ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
XM_005261977.1      ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
XM_005261979.1      ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
XM_005261978.1      ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
NM_007250.4         ATTACAGTCCCACTCATTGGAGGAGATGGTAAAAATGCTGGATCAGTGAAAGTTGACCCC
                    ************************************************************

HsCD00329464        ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
XM_005261977.1      ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
XM_005261979.1      ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
XM_005261978.1      ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
NM_007250.4         ACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGGAGAGTACAATTGAGAGTGGA
                    ************************************************************

HsCD00329464        TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
XM_005261977.1      TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
XM_005261979.1      TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
XM_005261978.1      TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
NM_007250.4         TCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAATGGCCCAAATG
                    ************************************************************

HsCD00329464        CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
XM_005261977.1      CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
XM_005261979.1      CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
XM_005261978.1      CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
NM_007250.4         CAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA
                    ************************************************************

HsCD00329464        TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
XM_005261977.1      TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
XM_005261979.1      TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
XM_005261978.1      TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
NM_007250.4         TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGA
                    ************************************************************

HsCD00329464        GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
XM_005261977.1      GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
XM_005261979.1      GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
XM_005261978.1      GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
NM_007250.4         GAGAAGCCTTATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAG
                    ************************************************************

HsCD00329464        CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
XM_005261977.1      CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
XM_005261979.1      CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
XM_005261978.1      CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
NM_007250.4         CTCACTCGCCATTTCCGCAAGCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAAC
                    ************************************************************

HsCD00329464        CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTTG
XM_005261977.1      CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTGA
XM_005261979.1      CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTGA
XM_005261978.1      CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTGA
NM_007250.4         CGCAGCTTTTCTCGTTCTGACCACCTGTCCCTGCATCGCCGTCGCCATGACACCATGTGA
                    ********************************************************** .

Pathways

NCI : Signaling events mediated by focal adhesion kinase

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : O95600
UniProt : B4DJN3
HPRD : 02238

Clone

Cloning Information : 268351
HIP Master Clone ID : 193209
Original Clone ID : FLH268351.01L

Organism


TAX_ID : 9606
Species Specific ID: 11279

Genome

Chromosome : X
Map Location : Xp11.21
Ensembl : ENSG00000102349

Reagents

Labome : KLF8-antibody