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LHX6 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LHX6
Gene Name:  LIM homeobox 6
Sequence             
Original Clone ID: FLH268353.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329466

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1092nts         Open reading frame : 1 to 1092
Reference Sequence Alignment

ATGGCCCAGCCAGGGTCCGGCTGCAAAGCGACCACCCGCTGTCTTGAAGGGACCGCGCCG
CCCGCCATGGCTCAGTCTGACCCCCAGGCCCTGGCAGGAGCTCTGGACAAGGACGAGGGT
CAGGCCTCCCCATGTACGCCCAGCACGCCATCTGTCTGCTCACCGCCCTCTGCCGCCTCC
TCCGTGCCGTCTGCAGGCAAGAACATCTGCTCCAGCTGCGGCCTCGAGATCCTGGACCGA
TATCTGCTCAAGGTCAACAACCTCATCTGGCACGTGCGGTGCCTCGAGTGCTCCGTGTGT
CGCACGTCGCTGAGGCAGCAGAACAGCTGCTACATCAAGAACAAGGAGATCTTCTGCAAG
ATGGACTACTTCAGCCGATTCGGGACCAAGTGTGCCCGGTGCGGCCGACAGATCTACGCC
AGCGACTGGGTGCGGAGAGCTCGCGGCAACGCCTACCACCTGGCCTGCTTCGCCTGCTTC
TCGTGCAAGCGCCAGCTGTCCACTGGTGAGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTC
TGCCGCATCCACTACGACACCATGATTGAGAACCTCAAGAGGGCCGCCGAGAACGGGAAC
GGCCTCACGTTGGAGGGGGCAGTGCCCTCGGAACAGGACAGTCAACCCAAGCCGGCCAAG
CGCGCGCGGACGTCCTTCACCGCGGAACAGCTGCAGGTTATGCAGGCGCAGTTCGCGCAG
GACAACAACCCCGACGCTCAGACGCTGCAGAAGCTGGCGGACATGACGGGCCTCAGCCGG
AGAGTCATCCAGGTGTGGTTTCAAAACTGCCGGGCGCGTCATAAAAAGCACACGCCGCAA
CACCCAGTGCCGCCCTCGGGGGCGCCCCCGTCCCGCCTTCCCTCCGCCCTGTCCGACGAC
ATCCACTACACCCCGTTCAGCAGCCCCGAGCGGGCGCGCATGGTCACCCTGCACGGCTAC
ATTGAGAGTCAGGTACAGTGCGGGCAGGTGCACTGCCGGCTGCCTTACACCGCACCCCCC
GTCCACCTCAAAGCCGATATGGATGGGCCGCTCTCCAACCGGGGTGAGAAGGTCATCCTT
TTTCAGTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1110
Start on reference sequence 19
Mutations:
None
Discrepancy:
g82c,A28P; g85c,E29Q
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LHX6
Symbol Nomenclature : LHX6
SYNONYM : LHX6.1
Designation : LIM homeodomain protein 6.1|LIM/homeobox protein Lhx6|LIM/homeobox protein Lhx6.1
Full Nomenclature : LIM homeobox 6
GENEID : 26468
Locus Tag : RP11-498E2.6
HGNC : 21735
MIM : 608215
Vega : OTTHUMG00000020601
Target GenBank: BC103937

 

Reference Sequence Alignment

  

HsCD00329466        ----------------ATGGCCCAGCCAGGGTCCGGCTGCAAAGCGACCACCCGCTGTCT
NM_001242334.1      ----------------ATGGCCCAGCCAGGGTCCGGCTGCAAAGCGACCACCCGCTGTCT
XM_005251915.1      ATGTACTGGAAGCATGAGAACGCCGCCCCGGCGTTGCCCGAGGGCTGCCGGCTGCCGGCC
NM_014368.4         ATGTACTGGAAGCATGAGAACGCCGCCCCGGCGTTGCCCGAGGGCTGCCGGCTGCCGGCC
                                    * ..* *.***. **    **   *..** .**. * ** * * 

HsCD00329466        TGAAGGGACCGCGCCGCCCGCCATG-----------------------------------
NM_001242334.1      TGAAGGGACCGCGCCGCCCGCCATG-----------------------------------
XM_005251915.1      GAGGGCGGCCCCGCCACCG-----ACCAG-------------------------------
NM_014368.4         GAGGGCGGCCCCGCCACCG-----ACCAGGTGATGGCCCAGCCAGGGTCCGGCTGCAAAG
                     ...* *.** ****.**      .                                   

HsCD00329466        -----------------------------------------GCTCAGTCTGACCCCCAGG
NM_001242334.1      -----------------------------------------GCTCAGTCTGACGCCGAGG
XM_005251915.1      -----------------------------------------GCTCAGTCTGACGCCGAGG
NM_014368.4         CGACCACCCGCTGTCTTGAAGGGACCGCGCCGCCCGCCATGGCTCAGTCTGACGCCGAGG
                                                             ************ ** ***

HsCD00329466        CCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAGGCCTCCCCATGTACGCCCAGCACGC
NM_001242334.1      CCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAGGCCTCCCCATGTACGCCCAGCACGC
XM_005251915.1      CCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAGGCCTCCCCATGTACGCCCAGCACGC
NM_014368.4         CCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAGGCCTCCCCATGTACGCCCAGCACGC
                    ************************************************************

HsCD00329466        CATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTGCAGGCAAGAACATCT
NM_001242334.1      CATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTGCAGGCAAGAACATCT
XM_005251915.1      CATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTGCAGGCAAGAACATCT
NM_014368.4         CATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTGCAGGCAAGAACATCT
                    ************************************************************

HsCD00329466        GCTCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCTCATCT
NM_001242334.1      GCTCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCTCATCT
XM_005251915.1      GCTCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCTCATCT
NM_014368.4         GCTCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCTCATCT
                    ************************************************************

HsCD00329466        GGCACGTGCGGTGCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCT
NM_001242334.1      GGCACGTGCGGTGCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCT
XM_005251915.1      GGCACGTGCGGTGCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCT
NM_014368.4         GGCACGTGCGGTGCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCT
                    ************************************************************

HsCD00329466        GCTACATCAAGAACAAGGAGATCTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCA
NM_001242334.1      GCTACATCAAGAACAAGGAGATCTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCA
XM_005251915.1      GCTACATCAAGAACAAGGAGATCTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCA
NM_014368.4         GCTACATCAAGAACAAGGAGATCTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCA
                    ************************************************************

HsCD00329466        AGTGTGCCCGGTGCGGCCGACAGATCTACGCCAGCGACTGGGTGCGGAGAGCTCGCGGCA
NM_001242334.1      AGTGTGCCCGGTGCGGCCGACAGATCTACGCCAGCGACTGGGTGCGGAGAGCTCGCGGCA
XM_005251915.1      AGTGTGCCCGGTGCGGCCGACAGATCTACGCCAGCGACTGGGTGCGGAGAGCTCGCGGCA
NM_014368.4         AGTGTGCCCGGTGCGGCCGACAGATCTACGCCAGCGACTGGGTGCGGAGAGCTCGCGGCA
                    ************************************************************

HsCD00329466        ACGCCTACCACCTGGCCTGCTTCGCCTGCTTCTCGTGCAAGCGCCAGCTGTCCACTGGTG
NM_001242334.1      ACGCCTACCACCTGGCCTGCTTCGCCTGCTTCTCGTGCAAGCGCCAGCTGTCCACTGGTG
XM_005251915.1      ACGCCTACCACCTGGCCTGCTTCGCCTGCTTCTCGTGCAAGCGCCAGCTGTCCACTGGTG
NM_014368.4         ACGCCTACCACCTGGCCTGCTTCGCCTGCTTCTCGTGCAAGCGCCAGCTGTCCACTGGTG
                    ************************************************************

HsCD00329466        AGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGCCGCATCCACTACGACACCATGATTG
NM_001242334.1      AGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGCCGCATCCACTACGACACCATGATTG
XM_005251915.1      AGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGCCGCATCCACTACGACACCATGATTG
NM_014368.4         AGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGCCGCATCCACTACGACACCATGATTG
                    ************************************************************

HsCD00329466        AGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGGAGGGGGCAGTGCCCT
NM_001242334.1      AGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGGAGGGGGCAGTGCCCT
XM_005251915.1      AGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGGAGGGGGCAGTGCCCT
NM_014368.4         AGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGGAGGGGGCAGTGCCCT
                    ************************************************************

HsCD00329466        CGGAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGCGGAAC
NM_001242334.1      CGGAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGCGGAAC
XM_005251915.1      CGGAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGCGGAAC
NM_014368.4         CGGAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGCGGAAC
                    ************************************************************

HsCD00329466        AGCTGCAGGTTATGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGC
NM_001242334.1      AGCTGCAGGTTATGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGC
XM_005251915.1      AGCTGCAGGTTATGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGC
NM_014368.4         AGCTGCAGGTTATGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGC
                    ************************************************************

HsCD00329466        AGAAGCTGGCGGACATGACGGGCCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACT
NM_001242334.1      AGAAGCTGGCGGACATGACGGGCCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACT
XM_005251915.1      AGAAGCTGGCGGACATGACGGGCCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACT
NM_014368.4         AGAAGCTGGCGGACATGACGGGCCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACT
                    ************************************************************

HsCD00329466        GCCGGGCGCGTCATAAAAAGCACACGCCGCAACACCCAGTGCCGCCCTCGGGGGCGCCCC
NM_001242334.1      GCCGGGCGCGTCATAAAAAGCACACGCCGCAACACCCAGTGCCGCCCTCGGGGGCGCCCC
XM_005251915.1      GCCGGGCGCGTCATAAAAAGCACACGCCGCAACACCCAGTGCCGCCCTCGGGGGCGCCCC
NM_014368.4         GCCGGGCGCGTCATAAAAAGCACACGCCGCAACACCCAGTGCCGCCCTCGGGGGCGCCCC
                    ************************************************************

HsCD00329466        CGTCCCGCCTTCCCTCCGCCCTGTCCGACGACATCCACTACACCCCGTTCAGCAGCCCCG
NM_001242334.1      CGTCCCGCCTTCCCTCCGCCCTGTCCGACGACATCCACTACACCCCGTTCAGCAGCCCCG
XM_005251915.1      CGTCCCGCCTTCCCTCCGCCCTGTCCGACGACATCCACTACACCCCGTTCAGCAGCCCCG
NM_014368.4         CGTCCCGCCTTCCCTCCGCCCTGTCCGACGACATCCACTACACCCCGTTCAGCAGCCCCG
                    ************************************************************

HsCD00329466        AGCGGGCGCGCATGGTCACCCTGCACGGCTACATTGAGAGTCAGGTACAGTGCGGGCAGG
NM_001242334.1      AGCGGGCGCGCATGGTCACCCTGCACGGCTACATTGAGAGTCAGGTACAGTGCGGGCAGG
XM_005251915.1      AGCGGGCGCGCATGGTCACCCTGCACGGCTACATTGAGAGTCAGGTACAGTGCGGGCAGG
NM_014368.4         AGCGGGCGCGCATGGTCACCCTGCACGGCTACATTGAGAGTCAGGTACAGTGCGGGCAGG
                    ************************************************************

HsCD00329466        TGCACTGCCGGCTGCCTTACACCGCACCCCCCGTCCACCTCAAAGCCGATATGGATGGGC
NM_001242334.1      TGCACTGCCGGCTGCCTTACACCGCACCCCCCGTCCACCTCAAAGCCGATATGGATGGGC
XM_005251915.1      TGCACTGCCGGCTGCCTTACACCGCACCCCCCGTCCACCTCAAAGCCGATATGGATGGGC
NM_014368.4         TGCACTGCCGGCTGCCTTACACCGCACCCCCCGTCCACCTCAAAGCCGATATGGATGGGC
                    ************************************************************

HsCD00329466        CGCTCTCCAACCGGGGTGAGAAGGTCATCCTTTTTCAGTACTTG
NM_001242334.1      CGCTCTCCAACCGGGGTGAGAAGGTCATCCTTTTTCAGTACTAA
XM_005251915.1      CGCTCTCCAACCGGGGTGAGAAGGTCATCCTTTTTCAGTACTAA
NM_014368.4         CGCTCTCCAACCGGGGTGAGAAGGTCATCCTTTTTCAGTACTAA
                    ******************************************:.

Protein

SMART domain : HOX : Homeodomain
SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : Q9UPM6
HPRD : 10498

Clone

Cloning Information : 268353
HIP Master Clone ID : 193211
Original Clone ID : FLH268353.01L

Organism


TAX_ID : 9606
Species Specific ID: 26468

Genome

Chromosome : 9
Map Location : 9q33.2
Ensembl : ENSG00000106852

Reagents

Labome : LHX6-antibody