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ZNF354B (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF354B
Gene Name:  zinc finger protein 354B
Sequence             
Original Clone ID: FLH268354.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329467

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1839nts         Open reading frame : 1 to 1839
Reference Sequence Alignment

ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACATTCGAGGACGTGGCT
GTGCTCTTTACCTGGGATGAGTGGAGAAAGCTGGCTCCTTCTCAGAGAAACTTGTACCGG
GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGACTCTCATTTACCAAACCA
AAAGTCATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACAGTTCT
GGTGTCTCCTCTCTAGGATGTAAGAGCACACCTAAAATGACAAAGTCAACTCAAACTCAG
GATTCATTTCAGGAGCAGATAAGGAAAAGATTGAAAAGGGATGAACCCTGGAACTTCATA
TCAGAAAGATCCTGCATATATGAAGAGAAATTAAAGAAACAGCAGGACAAAAATGAAAAT
TTACAAATAATTTCAGTTGCCCATACAAAAATCCTTACTGTAGATAGAAGCCATAAAAAT
GTTGAATTTGGCCAAAACTTCTACCTGAAATCAGTCTTCATTAAGCAACAGAGATTTGCT
AAAGAAAAAACTCCATCAAAATGTGAAATACAAAGAAATAGTTTCAAGCAGAATTCAAAT
TTACTTAACCAATCAAAAATCAAAACAGCAGAGAAACGCTATAAATGCAGTACATGTGAA
AAAGCCTTCATTCACAATTCATCCCTTCGTAAACATCAGAAAAACCACACTGGAGAAAAA
TTATTTAAATGTAAAGAATGTTTAAAAGCTTTCAGCCAAAGTTCTGCTCTTATTCAACAT
CAAAGAACTCATACAGGAGAGAAACCCTATATATGTAAAGAATGTGGGAAAGCCTTCAGC
CATAGTGCATCCCTTTGTAAGCATTTAAGGACCCATACTGTGGAGAAATGCTATAGATGT
AAAGAATGTGGTAAATCCTTCAGTCGAAGGTCTGGGCTTTTTATACATCAAAAAATCCAT
GCTCAAGAAAATCCCCATAAATACAATCCAGGTAGGAAGGCATCCAGTTACAGCACTTCC
CTTTCTGGAAGTCAGAAAATTCATCTCAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
TCTATTTCACGACTTAATAGACACCGAATAATTCATACTGGAGAGAAATTGTATAATTGT
AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCATGAGCGAATTCAT
ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCCGCT
CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAGTGTAACGAATGTGAC
AAAACATTCAGGTGTAACTCATCGCTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
CCATATCGATGTTTAGAATGTGGGATGTCTTTTGGCCAAAGTGCAGCTCTTATACAACAT
CAGAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
CAAAGCTCATCACTTATTGCACATCAAAGAATTCATACTGGAGAGAAACCCTATGAATGT
AATGCATGTGGGAAACTCTTTAGCCAGAGGTCATCCCTTACTAATCATTATAAAATTCAC
ATTGAAGAGGACTCCTTAAAAGCCGATTTGCATGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1896
Start on reference sequence 58
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF354B
Symbol Nomenclature : ZNF354B
SYNONYM : KID2
Full Nomenclature : zinc finger protein 354B
GENEID : 117608
HGNC : 17197
Target GenBank: BC112111

 

Reference Sequence Alignment

  

HsCD00329467      ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACATTCGAGGACGTGGCT
NM_058230.2       ATGGCTGCTGGGCAGCGGGAAGCGAGGCCCCAGGTGTCACTGACATTCGAGGACGTGGCT
                  ************************************************************

HsCD00329467      GTGCTCTTTACCTGGGATGAGTGGAGAAAGCTGGCTCCTTCTCAGAGAAACTTGTACCGG
NM_058230.2       GTGCTCTTTACCTGGGATGAGTGGAGAAAGCTGGCTCCTTCTCAGAGAAACTTGTACCGG
                  ************************************************************

HsCD00329467      GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGACTCTCATTTACCAAACCA
NM_058230.2       GATGTGATGCTGGAGAACTATAGGAACCTGGTCTCACTGGGACTCTCATTTACCAAACCA
                  ************************************************************

HsCD00329467      AAAGTCATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACAGTTCT
NM_058230.2       AAAGTCATCTCCCTGTTGCAGCAAGGAGAAGATCCCTGGGAGGTGGAGAAAGACAGTTCT
                  ************************************************************

HsCD00329467      GGTGTCTCCTCTCTAGGATGTAAGAGCACACCTAAAATGACAAAGTCAACTCAAACTCAG
NM_058230.2       GGTGTCTCCTCTCTAGGATGTAAGAGCACACCTAAAATGACAAAGTCAACTCAAACTCAG
                  ************************************************************

HsCD00329467      GATTCATTTCAGGAGCAGATAAGGAAAAGATTGAAAAGGGATGAACCCTGGAACTTCATA
NM_058230.2       GATTCATTTCAGGAGCAGATAAGGAAAAGATTGAAAAGGGATGAACCCTGGAACTTCATA
                  ************************************************************

HsCD00329467      TCAGAAAGATCCTGCATATATGAAGAGAAATTAAAGAAACAGCAGGACAAAAATGAAAAT
NM_058230.2       TCAGAAAGATCCTGCATATATGAAGAGAAATTAAAGAAACAGCAGGACAAAAATGAAAAT
                  ************************************************************

HsCD00329467      TTACAAATAATTTCAGTTGCCCATACAAAAATCCTTACTGTAGATAGAAGCCATAAAAAT
NM_058230.2       TTACAAATAATTTCAGTTGCCCATACAAAAATCCTTACTGTAGATAGAAGCCATAAAAAT
                  ************************************************************

HsCD00329467      GTTGAATTTGGCCAAAACTTCTACCTGAAATCAGTCTTCATTAAGCAACAGAGATTTGCT
NM_058230.2       GTTGAATTTGGCCAAAACTTCTACCTGAAATCAGTCTTCATTAAGCAACAGAGATTTGCT
                  ************************************************************

HsCD00329467      AAAGAAAAAACTCCATCAAAATGTGAAATACAAAGAAATAGTTTCAAGCAGAATTCAAAT
NM_058230.2       AAAGAAAAAACTCCATCAAAATGTGAAATACAAAGAAATAGTTTCAAGCAGAATTCAAAT
                  ************************************************************

HsCD00329467      TTACTTAACCAATCAAAAATCAAAACAGCAGAGAAACGCTATAAATGCAGTACATGTGAA
NM_058230.2       TTACTTAACCAATCAAAAATCAAAACAGCAGAGAAACGCTATAAATGCAGTACATGTGAA
                  ************************************************************

HsCD00329467      AAAGCCTTCATTCACAATTCATCCCTTCGTAAACATCAGAAAAACCACACTGGAGAAAAA
NM_058230.2       AAAGCCTTCATTCACAATTCATCCCTTCGTAAACATCAGAAAAACCACACTGGAGAAAAA
                  ************************************************************

HsCD00329467      TTATTTAAATGTAAAGAATGTTTAAAAGCTTTCAGCCAAAGTTCTGCTCTTATTCAACAT
NM_058230.2       TTATTTAAATGTAAAGAATGTTTAAAAGCTTTCAGCCAAAGTTCTGCTCTTATTCAACAT
                  ************************************************************

HsCD00329467      CAAAGAACTCATACAGGAGAGAAACCCTATATATGTAAAGAATGTGGGAAAGCCTTCAGC
NM_058230.2       CAAAGAACTCATACAGGAGAGAAACCCTATATATGTAAAGAATGTGGGAAAGCCTTCAGC
                  ************************************************************

HsCD00329467      CATAGTGCATCCCTTTGTAAGCATTTAAGGACCCATACTGTGGAGAAATGCTATAGATGT
NM_058230.2       CATAGTGCATCCCTTTGTAAGCATTTAAGGACCCATACTGTGGAGAAATGCTATAGATGT
                  ************************************************************

HsCD00329467      AAAGAATGTGGTAAATCCTTCAGTCGAAGGTCTGGGCTTTTTATACATCAAAAAATCCAT
NM_058230.2       AAAGAATGTGGTAAATCCTTCAGTCGAAGGTCTGGGCTTTTTATACATCAAAAAATCCAT
                  ************************************************************

HsCD00329467      GCTCAAGAAAATCCCCATAAATACAATCCAGGTAGGAAGGCATCCAGTTACAGCACTTCC
NM_058230.2       GCTCAAGAAAATCCCCATAAATACAATCCAGGCAGGAAGGCATCCAGTTACAGCACTTCC
                  ******************************** ***************************

HsCD00329467      CTTTCTGGAAGTCAGAAAATTCATCTCAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
NM_058230.2       CTTTCTGGAAGTCAGAAAATTCATCTCAGAAAGAAGTCCTACTTATGTAATGAATGTGGC
                  ************************************************************

HsCD00329467      AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
NM_058230.2       AACACCTTTAAGTCTAGCTCATCCCTTCGTTATCATCAGAGAATTCACACTGGAGAGAAG
                  ************************************************************

HsCD00329467      CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
NM_058230.2       CCTTTTAAATGTAGTGAATGTGGGAGAGCCTTCAGCCAGAGTGCCTCTCTTATTCAACAT
                  ************************************************************

HsCD00329467      GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
NM_058230.2       GAAAGAATTCACACCGGAGAAAAGCCCTATAGATGCAATGAATGTGGGAAAGGCTTTACT
                  ************************************************************

HsCD00329467      TCTATTTCACGACTTAATAGACACCGAATAATTCATACTGGAGAGAAATTGTATAATTGT
NM_058230.2       TCTATTTCACGACTTAATAGACACCGAATAATTCATACTGGAGAGAAATTGTATAATTGT
                  ************************************************************

HsCD00329467      AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCATGAGCGAATTCAT
NM_058230.2       AATGAATGTGGTAAAGCCTTAAGCTCCCACTCAACACTTATTATTCATGAGCGAATTCAT
                  ************************************************************

HsCD00329467      ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCCGCT
NM_058230.2       ACTGGAGAAAAACCATGTAAATGTAAAGTATGTGGAAAAGCCTTCAGACAGAGTTCCGCT
                  ************************************************************

HsCD00329467      CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAGTGTAACGAATGTGAC
NM_058230.2       CTCATTCAACATCAGAGAATGCATACTGGAGAAAGACCCTATAAGTGTAACGAATGTGAC
                  ************************************************************

HsCD00329467      AAAACATTCAGGTGTAACTCATCGCTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
NM_058230.2       AAAACATTCAGGTGTAACTCATCGCTTAGTAATCACCAGAGAATTCATACTGGAGAGAAA
                  ************************************************************

HsCD00329467      CCATATCGATGTTTAGAATGTGGGATGTCTTTTGGCCAAAGTGCAGCTCTTATACAACAT
NM_058230.2       CCATATCGATGTTTAGAATGTGGGATGTCTTTTGGCCAAAGTGCAGCTCTTATACAACAT
                  ************************************************************

HsCD00329467      CAGAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
NM_058230.2       CAGAGGATTCATACAGGAGAAAAACCCTTTAAATGTAATACATGTGGAAAAACTTTTAGA
                  ************************************************************

HsCD00329467      CAAAGCTCATCACTTATTGCACATCAAAGAATTCATACTGGAGAGAAACCCTATGAATGT
NM_058230.2       CAAAGCTCATCACTTATTGCACATCAAAGAATTCATACTGGAGAGAAACCCTATGAATGT
                  ************************************************************

HsCD00329467      AATGCATGTGGGAAACTCTTTAGCCAGAGGTCATCCCTTACTAATCATTATAAAATTCAC
NM_058230.2       AATGCATGTGGGAAACTCTTTAGCCAGAGGTCATCCCTTACTAATCATTATAAAATTCAC
                  ************************************************************

HsCD00329467      ATTGAAGAGGACTCCTTAAAAGCCGATTTGCATGTGTTG
NM_058230.2       ATTGAAGAGGACTCCTTAAAAGCCGATTTGCATGTGTGA
                  ************************************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : Q96LW1
HPRD : 15784

Clone

Cloning Information : 268354
HIP Master Clone ID : 193212
Original Clone ID : FLH268354.01L

Organism


TAX_ID : 9606
Species Specific ID: 117608

Genome

Chromosome : 5
Map Location : 5q35.3

Reagents

Labome : ZNF354B-antibody