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MBNL2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MBNL2
Gene Name:  muscleblind-like splicing regulator 2
Sequence             
Original Clone ID: FLH268355.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329468

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1104nts         Open reading frame : 1 to 1104
Reference Sequence Alignment

ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
GCCGCGGCCGCGGCCACAGTCATGGCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAG
AGACAAGCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCAC
TACCAGCAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAGGG
TCAGTTTTGTGCATGACACCCGCTACCAGTATTGTACCCATGATGCACAGCGCTACGTCC
GCCACTGTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACA
GCCAATCAGATAATTCTGAAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1872
Start on reference sequence 769
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MBNL2
Symbol Nomenclature : MBNL2
SYNONYM : MBLL; MBLL39; PRO2032
Designation : muscleblind-like 2|muscleblind-like protein 1|muscleblind-like protein 2|muscleblind-like protein-like 39
Full Nomenclature : muscleblind-like splicing regulator 2
GENEID : 10150
Locus Tag : RP11-128N14.1
HGNC : 16746
MIM : 607327
Vega : OTTHUMG00000017239
Target GenBank: BC104040

 

Reference Sequence Alignment

  

HsCD00329468        ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
NM_144778.3         ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
XM_005254022.1      ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
XM_005254018.1      ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
XM_005254021.1      ATGGCTTTGAACGTTGCCCCAGTCAGAGATACAAAATGGCTGACATTAGAAGTCTGCAGA
                    ************************************************************

HsCD00329468        CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
NM_144778.3         CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
XM_005254022.1      CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
XM_005254018.1      CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
XM_005254021.1      CAGTTTCAAAGAGGAACATGCTCACGCTCTGATGAAGAATGCAAATTTGCTCATCCCCCC
                    ************************************************************

HsCD00329468        AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
NM_144778.3         AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
XM_005254022.1      AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
XM_005254018.1      AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
XM_005254021.1      AAAAGTTGTCAGGTTGAAAATGGAAGAGTAATTGCCTGCTTTGATTCCCTAAAGGGCCGT
                    ************************************************************

HsCD00329468        TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
NM_144778.3         TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
XM_005254022.1      TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
XM_005254018.1      TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
XM_005254021.1      TGTTCGAGAGAGAACTGCAAGTATCTTCACCCTCCGACACACTTAAAAACTCAACTAGAA
                    ************************************************************

HsCD00329468        ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
NM_144778.3         ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
XM_005254022.1      ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
XM_005254018.1      ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
XM_005254021.1      ATTAATGGAAGGAACAATTTGATTCAGCAAAAAACTGCAGCAGCAATGCTTGCCCAGCAG
                    ************************************************************

HsCD00329468        ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
NM_144778.3         ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
XM_005254022.1      ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
XM_005254018.1      ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
XM_005254021.1      ATGCAATTTATGTTTCCAGGAACACCACTTCATCCAGTGCCCACTTTCCCTGTAGGTCCC
                    ************************************************************

HsCD00329468        GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
NM_144778.3         GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
XM_005254022.1      GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
XM_005254018.1      GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
XM_005254021.1      GCGATAGGGACAAATACGGCTATTAGCTTTGCTCCTTACCTAGCACCTGTAACCCCTGGA
                    ************************************************************

HsCD00329468        GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
NM_144778.3         GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
XM_005254022.1      GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
XM_005254018.1      GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
XM_005254021.1      GTTGGGTTGGTCCCAACGGAAATTCTGCCCACCACGCCTGTTATTGTTCCCGGAAGTCCA
                    ************************************************************

HsCD00329468        CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
NM_144778.3         CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
XM_005254022.1      CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
XM_005254018.1      CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
XM_005254021.1      CCGGTCACTGTCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGACAAACTGGAG
                    ************************************************************

HsCD00329468        GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
NM_144778.3         GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
XM_005254022.1      GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
XM_005254018.1      GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
XM_005254021.1      GTATGCAGGGAGTTCCAGCGAGGAAACTGTGCCCGGGGAGAGACCGACTGCCGCTTTGCA
                    ************************************************************

HsCD00329468        CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
NM_144778.3         CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
XM_005254022.1      CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
XM_005254018.1      CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
XM_005254021.1      CACCCCGCAGACAGCACCATGATCGACACAAGTGACAACACCGTAACCGTTTGTATGGAT
                    ************************************************************

HsCD00329468        TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
NM_144778.3         TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
XM_005254022.1      TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
XM_005254018.1      TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
XM_005254021.1      TACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATTTTCACCCTCCTGCACACTTG
                    ************************************************************

HsCD00329468        CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
NM_144778.3         CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
XM_005254022.1      CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
XM_005254018.1      CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
XM_005254021.1      CAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAAGCTGCGGTGGCCGCCCAGGCA
                    ************************************************************

HsCD00329468        GCCGCGGCCGCGGCCACAGTCATG------------------------------------
NM_144778.3         GCCGCGGCCGCGGCCACAGTCATG------------------------------------
XM_005254022.1      GCCGCGGCCGCGGCCACAGTCAT-------------------------------------
XM_005254018.1      GCCGCGGCCGCGGCCACAGTCATGACTCAGTCGACTGCCAAAGCAATGAAGCGACCTCTC
XM_005254021.1      GCCGCGGCCGCGGCCACAGTCATGACTCAGTCGACTGCCAAAGCAATGAAGCGACCTCTC
                    ***********************                                     

HsCD00329468        ------------------GCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAGAGACAA
NM_144778.3         ------------------GCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAGAGACAA
XM_005254022.1      -----------------GGCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAGAGACAA
XM_005254018.1      GAAGCAACTGTAGACCTGGCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAGAGACAA
XM_005254021.1      GAAGCAACTGTAGACCTGGCCTTTCCCCCTGGTGCTCTTCATCCTTTACCAAAGAGACAA
                                      ******************************************

HsCD00329468        GCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCACTACCAG
NM_144778.3         GCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCACTACCAG
XM_005254022.1      GCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCACTACCAG
XM_005254018.1      GCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCACTACCAG
XM_005254021.1      GCACTTGAAAAAAGCAATGGTACCAGCGCGGTCTTTAACCCCAGCGTCTTGCACTACCAG
                    ************************************************************

HsCD00329468        CAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAGGGTCAGTT
NM_144778.3         CAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAGGGTCAGTT
XM_005254022.1      CAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAG--------
XM_005254018.1      CAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAGGGTCAGTT
XM_005254021.1      CAGGCTCTCACCAGCGCACAGTTGCAGCAACACGCCGCGTTCATTCCAACAG--------
                    ****************************************************        

HsCD00329468        TTGTGCATGACACCCGCTACCAGTATTGTACCCATGATGCACAGCGCTACGTCCGCCACT
NM_144778.3         TTGTGCATGACACCCGCTACCAGTATTGTACCCATGATGCACAGCGCTACGTCCGCCACT
XM_005254022.1      ----------------------------TACCCATGATGCACAGCGCTACGTCCGCCACT
XM_005254018.1      TTGTGCATGACACCCGCTACCAGTATTGTACCCATGATGCACAGCGCTACGTCCGCCACT
XM_005254021.1      ----------------------------TACCCATGATGCACAGCGCTACGTCCGCCACT
                                                ********************************

HsCD00329468        GTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAAT
NM_144778.3         GTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAAT
XM_005254022.1      GTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAAT
XM_005254018.1      GTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAAT
XM_005254021.1      GTCTCTGCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAAT
                    ************************************************************

HsCD00329468        CAGATAATTCTGAAATTG
NM_144778.3         CAGATAATTCTGAAATAA
XM_005254022.1      CAGATAATTCTGAAATAA
XM_005254018.1      CAGATAATTCTGAAATAA
XM_005254021.1      CAGATAATTCTGAAATAA
                    ****************:.

Protein

SMART domain : ZnF_C3H1 : zinc finger
UniProt : A2A3S3
UniProt : Q5VZF2
HPRD : 09543

Clone

Cloning Information : 268355
HIP Master Clone ID : 193213
Original Clone ID : FLH268355.01L

Organism


TAX_ID : 9606
Species Specific ID: 10150

Genome

Chromosome : 13
Map Location : 13q32.1
Ensembl : ENSG00000139793

Reagents

Labome : MBNL2-antibody