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LHX2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LHX2
Gene Name:  LIM homeobox 2
Sequence             
Original Clone ID: FLH268362.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329475

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1221nts         Open reading frame : 1 to 1221
Reference Sequence Alignment

ATGCTGTTCCACAGTCTGTCGGGCCCCGAGGTGCACGGGGTCATCGACGAGATGGACCGC
AGGGCCAAGAGCGAGGCTCCCGCCATCAGCTCCGCCATCGACCGCGGCGACACCGAGACG
ACCATGCCGTCCATCAGCAGTGACCGCGCCGCGCTGTGCGCCGGCTGCGGGGGCAAGATC
TCGGACCGCTACTACCTGCTGGCGGTGGACAAGCAGTGGCACATGCGCTGCCTCAAGTGC
TGCGAGTGCAAGCTCAACCTGGAGTCGGAGCTCACCTGTTTCAGCAAGGACGGTAGCATC
TACTGCAAGGAAGACTACTACAGGCGCTTCTCTGTGCAGCGCTGCGCCCGCTGCCACCTG
GGCATCTCGGCCTCGGAGATGGTGATGCGCGCTCGGGACTTGGTTTATCACCTCAACTGC
TTCACGTGCACCACGTGTAACAAGATGCTGACCACGGGCGACCACTTCGGCATGAAGGAC
AGCCTGGTCTACTGCCGCTTGCACTTCGAGGCGCTGCTGCAGGGCGAGTACCCCGCACAC
TTCAACCATGCCGACGTGGCAGCGGCGGCCGCTGCAGCCGCGGCGGCCAAGAGCGCGGGG
CTGGGCGCAGCAGGGGCCAACCCTCTGGGTCTTCCCTACTACAATGGCGTGGGCACTGTG
CAGAAGGGGCGGCCGAGGAAACGTAAGAGCCCGGGCCCCGGTGCGGATCTGGCGGCCTAC
AACGCTGCGCTAAGCTGCAACGAAAACGACGCAGAGCACCTGGACCGTGACCAGCCATAC
CCGAGCAGCCAGAAGACCAAGCGCATGCGCACGTCCTTCAAGCACCACCAGCTTCGGACC
ATGAAGTCTTACTTTGCCATTAACCACAACCCCGACGCCAAGGACTTGAAGCAGCTCGCG
CAAAAGACGGGCCTCACCAAGCGGGTCCTCCAGGTCTGGTTCCAGAACGCCCGAGCCAAG
TTCAGGCGCAACCTCTTACGGCAGGAAAACACGGGCGTGGACAAGTCGACAGACGCGGCG
CTGCAGACAGGGACGCCATCGGGCCCGGCCTCGGAGCTCTCCAACGCCTCGCTCAGCCCC
TCCAGCACGCCCACCACCCTGACAGACTTGACTAGCCCCACCCTGCCAACTGTGACGTCC
GTCTTAACTTCTGTGCCTGGCAACCTGGAGGGCCATGAGCCTCACAGCCCCTCACAAACG
ACTCTTACCAACCTTTTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1300
Start on reference sequence 80
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LHX2
Symbol Nomenclature : LHX2
SYNONYM : LH2; hLhx2
Designation : LIM HOX gene 2|LIM homeobox protein 2|LIM/homeobox protein Lhx2|homeobox protein LH-2
Full Nomenclature : LIM homeobox 2
GENEID : 9355
HGNC : 6594
MIM : 603759
Vega : OTTHUMG00000020647
Target GenBank: BC112185

 

Reference Sequence Alignment

  

HsCD00329475      ATGCTGTTCCACAGTCTGTCGGGCCCCGAGGTGCACGGGGTCATCGACGAGATGGACCGC
NM_004789.3       ATGCTGTTCCACAGTCTGTCGGGCCCCGAGGTGCACGGGGTCATCGACGAGATGGACCGC
                  ************************************************************

HsCD00329475      AGGGCCAAGAGCGAGGCTCCCGCCATCAGCTCCGCCATCGACCGCGGCGACACCGAGACG
NM_004789.3       AGGGCCAAGAGCGAGGCTCCCGCCATCAGCTCCGCCATCGACCGCGGCGACACCGAGACG
                  ************************************************************

HsCD00329475      ACCATGCCGTCCATCAGCAGTGACCGCGCCGCGCTGTGCGCCGGCTGCGGGGGCAAGATC
NM_004789.3       ACCATGCCGTCCATCAGCAGTGACCGCGCCGCGCTGTGCGCCGGCTGCGGGGGCAAGATC
                  ************************************************************

HsCD00329475      TCGGACCGCTACTACCTGCTGGCGGTGGACAAGCAGTGGCACATGCGCTGCCTCAAGTGC
NM_004789.3       TCGGACCGCTACTACCTGCTGGCGGTGGACAAGCAGTGGCACATGCGCTGCCTCAAGTGC
                  ************************************************************

HsCD00329475      TGCGAGTGCAAGCTCAACCTGGAGTCGGAGCTCACCTGTTTCAGCAAGGACGGTAGCATC
NM_004789.3       TGCGAGTGCAAGCTCAACCTGGAGTCGGAGCTCACCTGTTTCAGCAAGGACGGTAGCATC
                  ************************************************************

HsCD00329475      TACTGCAAGGAAGACTACTACAGGCGCTTCTCTGTGCAGCGCTGCGCCCGCTGCCACCTG
NM_004789.3       TACTGCAAGGAAGACTACTACAGGCGCTTCTCTGTGCAGCGCTGCGCCCGCTGCCACCTG
                  ************************************************************

HsCD00329475      GGCATCTCGGCCTCGGAGATGGTGATGCGCGCTCGGGACTTGGTTTATCACCTCAACTGC
NM_004789.3       GGCATCTCGGCCTCGGAGATGGTGATGCGCGCTCGGGACTTGGTTTATCACCTCAACTGC
                  ************************************************************

HsCD00329475      TTCACGTGCACCACGTGTAACAAGATGCTGACCACGGGCGACCACTTCGGCATGAAGGAC
NM_004789.3       TTCACGTGCACCACGTGTAACAAGATGCTGACCACGGGCGACCACTTCGGCATGAAGGAC
                  ************************************************************

HsCD00329475      AGCCTGGTCTACTGCCGCTTGCACTTCGAGGCGCTGCTGCAGGGCGAGTACCCCGCACAC
NM_004789.3       AGCCTGGTCTACTGCCGCTTGCACTTCGAGGCGCTGCTGCAGGGCGAGTACCCCGCACAC
                  ************************************************************

HsCD00329475      TTCAACCATGCCGACGTGGCAGCGGCGGCCGCTGCAGCCGCGGCGGCCAAGAGCGCGGGG
NM_004789.3       TTCAACCATGCCGACGTGGCAGCGGCGGCCGCTGCAGCCGCGGCGGCCAAGAGCGCGGGG
                  ************************************************************

HsCD00329475      CTGGGCGCAGCAGGGGCCAACCCTCTGGGTCTTCCCTACTACAATGGCGTGGGCACTGTG
NM_004789.3       CTGGGCGCAGCAGGGGCCAACCCTCTGGGTCTTCCCTACTACAATGGCGTGGGCACTGTG
                  ************************************************************

HsCD00329475      CAGAAGGGGCGGCCGAGGAAACGTAAGAGCCCGGGCCCCGGTGCGGATCTGGCGGCCTAC
NM_004789.3       CAGAAGGGGCGGCCGAGGAAACGTAAGAGCCCGGGCCCCGGTGCGGATCTGGCGGCCTAC
                  ************************************************************

HsCD00329475      AACGCTGCGCTAAGCTGCAACGAAAACGACGCAGAGCACCTGGACCGTGACCAGCCATAC
NM_004789.3       AACGCTGCGCTAAGCTGCAACGAAAACGACGCAGAGCACCTGGACCGTGACCAGCCATAC
                  ************************************************************

HsCD00329475      CCGAGCAGCCAGAAGACCAAGCGCATGCGCACGTCCTTCAAGCACCACCAGCTTCGGACC
NM_004789.3       CCGAGCAGCCAGAAGACCAAGCGCATGCGCACGTCCTTCAAGCACCACCAGCTTCGGACC
                  ************************************************************

HsCD00329475      ATGAAGTCTTACTTTGCCATTAACCACAACCCCGACGCCAAGGACTTGAAGCAGCTCGCG
NM_004789.3       ATGAAGTCTTACTTTGCCATTAACCACAACCCCGACGCCAAGGACTTGAAGCAGCTCGCG
                  ************************************************************

HsCD00329475      CAAAAGACGGGCCTCACCAAGCGGGTCCTCCAGGTCTGGTTCCAGAACGCCCGAGCCAAG
NM_004789.3       CAAAAGACGGGCCTCACCAAGCGGGTCCTCCAGGTCTGGTTCCAGAACGCCCGAGCCAAG
                  ************************************************************

HsCD00329475      TTCAGGCGCAACCTCTTACGGCAGGAAAACACGGGCGTGGACAAGTCGACAGACGCGGCG
NM_004789.3       TTCAGGCGCAACCTCTTACGGCAGGAAAACACGGGCGTGGACAAGTCGACAGACGCGGCG
                  ************************************************************

HsCD00329475      CTGCAGACAGGGACGCCATCGGGCCCGGCCTCGGAGCTCTCCAACGCCTCGCTCAGCCCC
NM_004789.3       CTGCAGACAGGGACGCCATCGGGCCCGGCCTCGGAGCTCTCCAACGCCTCGCTCAGCCCC
                  ************************************************************

HsCD00329475      TCCAGCACGCCCACCACCCTGACAGACTTGACTAGCCCCACCCTGCCAACTGTGACGTCC
NM_004789.3       TCCAGCACGCCCACCACCCTGACAGACTTGACTAGCCCCACCCTGCCAACTGTGACGTCC
                  ************************************************************

HsCD00329475      GTCTTAACTTCTGTGCCTGGCAACCTGGAGGGCCATGAGCCTCACAGCCCCTCACAAACG
NM_004789.3       GTCTTAACTTCTGTGCCTGGCAACCTGGAGGGCCATGAGCCTCACAGCCCCTCACAAACG
                  ************************************************************

HsCD00329475      ACTCTTACCAACCTTTTCTTG
NM_004789.3       ACTCTTACCAACCTTTTCTAA
                  *******************:.

Pathways

WikiPathway : Neural Crest Differentiation

Protein

SMART domain : HOX : Homeodomain
SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : B3KNJ5
UniProt : P50458
HPRD : 04786

Clone

Cloning Information : 268362
HIP Master Clone ID : 193223
Original Clone ID : FLH268362.01L

Organism


TAX_ID : 9606
Species Specific ID: 9355

Genome

Chromosome : 9
Map Location : 9q33.3
Ensembl : ENSG00000106689

Reagents

Labome : LHX2-antibody