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BHLHB2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BHLHE40
Gene Name:  basic helix-loop-helix family, member e40
Sequence             
Original Clone ID: FLH268367.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329479

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1239nts         Open reading frame : 1 to 1239
Reference Sequence Alignment

ATGGAGCGGATCCCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTG
GAGCACGGAGACCTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGA
CGGGGAATAAAGCGGAGCGAGGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATC
GAGAAAAAGAGACGTGACCGGATTAACGAGTGCATCGCCCAGCTGAAGGATCTCCTACCC
GAACATCTCAAACTTACAACTTTGGGTCACTTGGAAAAAGCAGTGGTTCTTGAACTTACC
TTGAAGCATGTGAAAGCACTAACAAACCTAATTGATCAGCAGCAGCAGAAAATCATTGCC
CTGCAGAGTGGTTTACAAGCTGGTGAGCTGTCAGGGAGAAATGTCGAAACAGGTCAAGAG
ATGTTCTGCTCAGGTTTCCAGACATGTGCCCGGGAGGTGCTTCAGTATCTGGCCAAGCAC
GAGAACACTCGGGACCTGAAGTCTTCGCAGCTTGTCACCCACCTCCACCGGGTGGTCTCG
GAGCTGCTGCAGGGTGGTACCTCCAGGAAGCCATCAGACCCAGCTCCCAAAGTGATGGAC
TTCAAGGAAAAACCCAGCTCTCCGGCCAAAGGTTCGGAAGGTCCTGGGAAAAACTGCGTG
CCAGTCATCCAGCGGACTTTCGCTCACTCGAGTGGGGAGCAGAGCGGCAGCGACACGGAC
ACAGACAGTGGCTATGGAGGAGAATCGGAGAAGGGCGACTTGCGCAGTGAGCAGCCGTGC
TTCAAAAGTGACCACGGACGCAGGTTCACGATGGGAGAAAGGATCGGCGCAATTAAGCAA
GAGTCCGAAGAACCCCCCACAAAAAAGAACCGGATGCAGCTTTCGGATGATGAAGGCCAT
TTCACTAGCAGTGACCTGATCAGCTCCCCGTTCCTGGGCCCACACCCACACCAGCCTCCT
TTCTGCCTGCCCTTCTACCTGATCCCACCTTCAGCGACTGCCTACCTGCCCATGCTGGAG
AAGTGCTGGTATCCCACCTCAGTGCCAGTGCTATACCCAGGCCTCAACGCCTCTGCCGCA
GCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTGCTCATGCCCCAGAGA
CTCCCTTCTCCCTTGCCAGCTCATCCGTCCGTCGACTCTTCTGTCTTGCTCCAAGCTCTG
AAGCCAATCCCCCCTTTAAACTTAGAAACCAAAGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1420
Start on reference sequence 182
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BHLHE40
Symbol Nomenclature : BHLHE40
SYNONYM : BHLHB2; DEC1; HLHB2; SHARP-2; STRA13; Stra14
Designation : basic helix-loop-helix domain containing, class B, 2|class B basic helix-loop-helix protein 2|class E basic helix-loop-helix protein 40|differentially expressed in chondrocytes 1|differentially expressed in chondrocytes protein 1|differentiated embryo chondrocyte expressed gene 1|enhancer-of-split and hairy-related protein 2|stimulated by retinoic acid gene 13 protein
Full Nomenclature : basic helix-loop-helix family, member e40
GENEID : 8553
HGNC : 1046
MIM : 604256
Vega : OTTHUMG00000119035
Target GenBank: BC082238

 

Reference Sequence Alignment

  

HsCD00329479      ATGGAGCGGATCCCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTG
NM_003670.2       ATGGAGCGGATCCCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTG
                  ************************************************************

HsCD00329479      GAGCACGGAGACCTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGA
NM_003670.2       GAGCACGGAGACCTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGA
                  ************************************************************

HsCD00329479      CGGGGAATAAAGCGGAGCGAGGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATC
NM_003670.2       CGGGGAATAAAGCGGAGCGAGGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATC
                  ************************************************************

HsCD00329479      GAGAAAAAGAGACGTGACCGGATTAACGAGTGCATCGCCCAGCTGAAGGATCTCCTACCC
NM_003670.2       GAGAAAAAGAGACGTGACCGGATTAACGAGTGCATCGCCCAGCTGAAGGATCTCCTACCC
                  ************************************************************

HsCD00329479      GAACATCTCAAACTTACAACTTTGGGTCACTTGGAAAAAGCAGTGGTTCTTGAACTTACC
NM_003670.2       GAACATCTCAAACTTACAACTTTGGGTCACTTGGAAAAAGCAGTGGTTCTTGAACTTACC
                  ************************************************************

HsCD00329479      TTGAAGCATGTGAAAGCACTAACAAACCTAATTGATCAGCAGCAGCAGAAAATCATTGCC
NM_003670.2       TTGAAGCATGTGAAAGCACTAACAAACCTAATTGATCAGCAGCAGCAGAAAATCATTGCC
                  ************************************************************

HsCD00329479      CTGCAGAGTGGTTTACAAGCTGGTGAGCTGTCAGGGAGAAATGTCGAAACAGGTCAAGAG
NM_003670.2       CTGCAGAGTGGTTTACAAGCTGGTGAGCTGTCAGGGAGAAATGTCGAAACAGGTCAAGAG
                  ************************************************************

HsCD00329479      ATGTTCTGCTCAGGTTTCCAGACATGTGCCCGGGAGGTGCTTCAGTATCTGGCCAAGCAC
NM_003670.2       ATGTTCTGCTCAGGTTTCCAGACATGTGCCCGGGAGGTGCTTCAGTATCTGGCCAAGCAC
                  ************************************************************

HsCD00329479      GAGAACACTCGGGACCTGAAGTCTTCGCAGCTTGTCACCCACCTCCACCGGGTGGTCTCG
NM_003670.2       GAGAACACTCGGGACCTGAAGTCTTCGCAGCTTGTCACCCACCTCCACCGGGTGGTCTCG
                  ************************************************************

HsCD00329479      GAGCTGCTGCAGGGTGGTACCTCCAGGAAGCCATCAGACCCAGCTCCCAAAGTGATGGAC
NM_003670.2       GAGCTGCTGCAGGGTGGTACCTCCAGGAAGCCATCAGACCCAGCTCCCAAAGTGATGGAC
                  ************************************************************

HsCD00329479      TTCAAGGAAAAACCCAGCTCTCCGGCCAAAGGTTCGGAAGGTCCTGGGAAAAACTGCGTG
NM_003670.2       TTCAAGGAAAAACCCAGCTCTCCGGCCAAAGGTTCGGAAGGTCCTGGGAAAAACTGCGTG
                  ************************************************************

HsCD00329479      CCAGTCATCCAGCGGACTTTCGCTCACTCGAGTGGGGAGCAGAGCGGCAGCGACACGGAC
NM_003670.2       CCAGTCATCCAGCGGACTTTCGCTCACTCGAGTGGGGAGCAGAGCGGCAGCGACACGGAC
                  ************************************************************

HsCD00329479      ACAGACAGTGGCTATGGAGGAGAATCGGAGAAGGGCGACTTGCGCAGTGAGCAGCCGTGC
NM_003670.2       ACAGACAGTGGCTATGGAGGAGAATCGGAGAAGGGCGACTTGCGCAGTGAGCAGCCGTGC
                  ************************************************************

HsCD00329479      TTCAAAAGTGACCACGGACGCAGGTTCACGATGGGAGAAAGGATCGGCGCAATTAAGCAA
NM_003670.2       TTCAAAAGTGACCACGGACGCAGGTTCACGATGGGAGAAAGGATCGGCGCAATTAAGCAA
                  ************************************************************

HsCD00329479      GAGTCCGAAGAACCCCCCACAAAAAAGAACCGGATGCAGCTTTCGGATGATGAAGGCCAT
NM_003670.2       GAGTCCGAAGAACCCCCCACAAAAAAGAACCGGATGCAGCTTTCGGATGATGAAGGCCAT
                  ************************************************************

HsCD00329479      TTCACTAGCAGTGACCTGATCAGCTCCCCGTTCCTGGGCCCACACCCACACCAGCCTCCT
NM_003670.2       TTCACTAGCAGTGACCTGATCAGCTCCCCGTTCCTGGGCCCACACCCACACCAGCCTCCT
                  ************************************************************

HsCD00329479      TTCTGCCTGCCCTTCTACCTGATCCCACCTTCAGCGACTGCCTACCTGCCCATGCTGGAG
NM_003670.2       TTCTGCCTGCCCTTCTACCTGATCCCACCTTCAGCGACTGCCTACCTGCCCATGCTGGAG
                  ************************************************************

HsCD00329479      AAGTGCTGGTATCCCACCTCAGTGCCAGTGCTATACCCAGGCCTCAACGCCTCTGCCGCA
NM_003670.2       AAGTGCTGGTATCCCACCTCAGTGCCAGTGCTATACCCAGGCCTCAACGCCTCTGCCGCA
                  ************************************************************

HsCD00329479      GCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTGCTCATGCCCCAGAGA
NM_003670.2       GCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTGCTCATGCCCCAGAGA
                  ************************************************************

HsCD00329479      CTCCCTTCTCCCTTGCCAGCTCATCCGTCCGTCGACTCTTCTGTCTTGCTCCAAGCTCTG
NM_003670.2       CTCCCTTCTCCCTTGCCAGCTCATCCGTCCGTCGACTCTTCTGTCTTGCTCCAAGCTCTG
                  ************************************************************

HsCD00329479      AAGCCAATCCCCCCTTTAAACTTAGAAACCAAAGACTTG
NM_003670.2       AAGCCAATCCCCCCTTTAAACTTAGAAACCAAAGACTAA
                  *************************************:.

Pathways

NCI : Circadian rhythm pathway
NCI : HIF-1-alpha transcription factor network
NCI : HIF-2-alpha transcription factor network
Reactome : BMAL1:CLOCK,NPAS2 activates circadian gene expression
Reactome : Circadian Clock
WikiPathway : Heart Development

Protein

SMART domain : HLH : helix loop helix domain
SMART domain : ORANGE : Orange domain
UniProt : Q6IB83
UniProt : O14503
HPRD : 16050

Clone

Cloning Information : 268367
HIP Master Clone ID : 193229
Original Clone ID : FLH268367.01L

Organism


TAX_ID : 9606
Species Specific ID: 8553

Genome

Chromosome : 3
Map Location : 3p26
Ensembl : ENSG00000134107

Reagents

Labome : BHLHE40-antibody