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FLJ25476 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  ZNF362
Gene Name:  zinc finger protein 362
Sequence             
Original Clone ID: FLH268371.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329482

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1263nts         Open reading frame : 1 to 1263
Reference Sequence Alignment

ATGAGTAGAAGTTCACCAAGTGGGAAAGGACACTCTAGGATGGCCGAGCCTCGATTTAAC
AACCCCTACTTCTGGCCCCCTCCTCCCACCATGCCCAGCCAGCTGGACAACCTGGTTCTG
ATTAACAAGATCAAGGAGCAGCTGATGGCCGAGAAGATCAGGCCGCCTCACCTGCCGCCC
ACGTCGGCCTCGTCGCAGCAGCCGTTGCTAGTGCCGCCGGCACCCGCCGAGAGCAGCCAG
GCCGTCATGTCGCTGCCCAAGCTGCAGCAGGTGCCGGGGCTGCATCCACAGGCGGTGCCG
CAGCCCGACGTGGCGCTGCACGCACGGCCGGCCACCAGCACCGTCACAGGTCTGGGGCTG
TCCACCCGGACCCCGTCTGTGAGCACTTCTGAGTCAAGCGCGGGCGCGGGCACGGGCACG
GGTACCAGCACCCCGTCCACACCCACCACCACCAGCCAGAGCCGCCTCATCGCCTCGTCC
CCCACCCTCATCTCAGGGATCACCAGCCCCCCTCTCCTGGACTCCATCAAGACAATCCAG
GGCCACGGCCTGCTTGGCCCCCCCAAGTCCGAACGCGGCCGCAAAAAGATCAAGGCGGAG
AACCCGGGGGGTCCGCCTGTCCTTGTAGTCCCCTATCCCATCCTGGCCTCGGGCGAGACT
GCCAAGGAGGGCAAGACGTACAGGTGTAAGGTATGCCCACTGACCTTTTTCACCAAGTCA
GAGATGCAGATCCACTCCAAGTCGCACACAGAGGCCAAGCCCCACAAGTGCCCGCACTGC
TCCAAGTCCTTTGCCAACGCCTCCTACCTGGCCCAGCACCTGCGCATCCACCTGGGCGTC
AAGCCCTACCACTGCTCCTACTGTGATAAGTCCTTCCGGCAGCTCTCCCACCTCCAGCAG
CACACCAGAATCCACACAGGCGACAGACCCTACAAGTGCCCACATCCTGGCTGCGAGAAG
GCTTTCACTCAGCTCTCCAACCTCCAGTCTCACCAGCGCCAGCACAACAAGGACAAGCCC
TACAAGTGTCCCAACTGCTACCGGGCCTATTCGGACTCCGCTTCTTTGCAGATCCACCTC
TCGGCCCACGCCATCAAGCACGCCAAGGCCTACTGCTGCAGCATGTGTGGGCGGGCCTAC
ACCTCGGAGACCTACCTGATGAAGCACATGTCCAAACACACGGTGGTGGAGCACCTGGTG
AGCCATCACTCGCCCCAGAGGACGGAGTCCCCCGGCATCCCGGTGCGAATCTCTCTCATC
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1355
Start on reference sequence 93
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF362
Symbol Nomenclature : ZNF362
SYNONYM : RN; lin-29
Designation : rotund homolog
Full Nomenclature : zinc finger protein 362
GENEID : 149076
Locus Tag : RP11-415J8.1
HGNC : 18079
Vega : OTTHUMG00000004124
Target GenBank: BC112113

 

Reference Sequence Alignment

  

HsCD00329482        ATGAGTAGAAGTTCACCAAGTGGGAAAGGACACTCTAGGATGGCCGAGCCTCGATTTAAC
XM_005270507.1      ---------------------------------------ATGGCCGAGCCTCGATTTAAC
NM_152493.2         ATGAGTAGAAGTTCACCAAGTGGGAAAGGACACTCTAGGATGGCCGAGCCTCGATTTAAC
                                                           *********************

HsCD00329482        AACCCCTACTTCTGGCCCCCTCCTCCCACCATGCCCAGCCAGCTGGACAACCTGGTTCTG
XM_005270507.1      AACCCCTACTTCTGGCCCCCTCCTCCCACCATGCCCAGCCAGCTGGACAACCTGGTTCTG
NM_152493.2         AACCCCTACTTCTGGCCCCCTCCTCCCACCATGCCCAGCCAGCTGGACAACCTGGTTCTG
                    ************************************************************

HsCD00329482        ATTAACAAGATCAAGGAGCAGCTGATGGCCGAGAAGATCAGGCCGCCTCACCTGCCGCCC
XM_005270507.1      ATTAACAAGATCAAGGAGCAGCTGATGGCCGAGAAGATCAGGCCGCCTCACCTGCCGCCC
NM_152493.2         ATTAACAAGATCAAGGAGCAGCTGATGGCCGAGAAGATCAGGCCGCCTCACCTGCCGCCC
                    ************************************************************

HsCD00329482        ACGTCGGCCTCGTCGCAGCAGCCGTTGCTAGTGCCGCCGGCACCCGCCGAGAGCAGCCAG
XM_005270507.1      ACGTCGGCCTCGTCGCAGCAGCCGTTGCTAGTGCCGCCGGCACCCGCCGAGAGCAGCCAG
NM_152493.2         ACGTCGGCCTCGTCGCAGCAGCCGTTGCTAGTGCCGCCGGCACCCGCCGAGAGCAGCCAG
                    ************************************************************

HsCD00329482        GCCGTCATGTCGCTGCCCAAGCTGCAGCAGGTGCCGGGGCTGCATCCACAGGCGGTGCCG
XM_005270507.1      GCCGTCATGTCGCTGCCCAAGCTGCAGCAGGTGCCGGGGCTGCATCCACAGGCGGTGCCG
NM_152493.2         GCCGTCATGTCGCTGCCCAAGCTGCAGCAGGTGCCGGGGCTGCATCCACAGGCGGTGCCG
                    ************************************************************

HsCD00329482        CAGCCCGACGTGGCGCTGCACGCACGGCCGGCCACCAGCACCGTCACAGGTCTGGGGCTG
XM_005270507.1      CAGCCCGACGTGGCGCTGCACGCACGGCCGGCCACCAGCACCGTCACAGGTCTGGGGCTG
NM_152493.2         CAGCCCGACGTGGCGCTGCACGCACGGCCGGCCACCAGCACCGTCACAGGTCTGGGGCTG
                    ************************************************************

HsCD00329482        TCCACCCGGACCCCGTCTGTGAGCACTTCTGAGTCAAGCGCGGGCGCGGGCACGGGCACG
XM_005270507.1      TCCACCCGGACCCCGTCTGTGAGCACTTCTGAGTCAAGCGCGGGCGCGGGCACGGGCACG
NM_152493.2         TCCACCCGGACCCCGTCTGTGAGCACTTCTGAGTCAAGCGCGGGCGCGGGCACGGGCACG
                    ************************************************************

HsCD00329482        GGTACCAGCACCCCGTCCACACCCACCACCACCAGCCAGAGCCGCCTCATCGCCTCGTCC
XM_005270507.1      GGTACCAGCACCCCGTCCACACCCACCACCACCAGCCAGAGCCGCCTCATCGCCTCGTCC
NM_152493.2         GGTACCAGCACCCCGTCCACACCCACCACCACCAGCCAGAGCCGCCTCATCGCCTCGTCC
                    ************************************************************

HsCD00329482        CCCACCCTCATCTCAGGGATCACCAGCCCCCCTCTCCTGGACTCCATCAAGACAATCCAG
XM_005270507.1      CCCACCCTCATCTCAGGGATCACCAGCCCCCCTCTCCTGGACTCCATCAAGACAATCCAG
NM_152493.2         CCCACCCTCATCTCAGGGATCACCAGCCCCCCTCTCCTGGACTCCATCAAGACAATCCAG
                    ************************************************************

HsCD00329482        GGCCACGGCCTGCTTGGCCCCCCCAAGTCCGAACGCGGCCGCAAAAAGATCAAGGCGGAG
XM_005270507.1      GGCCACGGCCTGCTTGGCCCCCCCAAGTCCGAACGCGGCCGCAAAAAGATCAAGGCGGAG
NM_152493.2         GGCCACGGCCTGCTTGGCCCCCCCAAGTCCGAACGCGGCCGCAAAAAGATCAAGGCGGAG
                    ************************************************************

HsCD00329482        AACCCGGGGGGTCCGCCTGTCCTTGTAGTCCCCTATCCCATCCTGGCCTCGGGCGAGACT
XM_005270507.1      AACCCGGGGGGTCCGCCTGTCCTTGTAGTCCCCTATCCCATCCTGGCCTCGGGCGAGACT
NM_152493.2         AACCCGGGGGGTCCGCCTGTCCTTGTAGTCCCCTATCCCATCCTGGCCTCGGGCGAGACT
                    ************************************************************

HsCD00329482        GCCAAGGAGGGCAAGACGTACAGGTGTAAGGTATGCCCACTGACCTTTTTCACCAAGTCA
XM_005270507.1      GCCAAGGAGGGCAAGACGTACAGGTGTAAGGTATGCCCACTGACCTTTTTCACCAAGTCA
NM_152493.2         GCCAAGGAGGGCAAGACGTACAGGTGTAAGGTATGCCCACTGACCTTTTTCACCAAGTCA
                    ************************************************************

HsCD00329482        GAGATGCAGATCCACTCCAAGTCGCACACAGAGGCCAAGCCCCACAAGTGCCCGCACTGC
XM_005270507.1      GAGATGCAGATCCACTCCAAGTCGCACACAGAGGCCAAGCCCCACAAGTGCCCGCACTGC
NM_152493.2         GAGATGCAGATCCACTCCAAGTCGCACACAGAGGCCAAGCCCCACAAGTGCCCGCACTGC
                    ************************************************************

HsCD00329482        TCCAAGTCCTTTGCCAACGCCTCCTACCTGGCCCAGCACCTGCGCATCCACCTGGGCGTC
XM_005270507.1      TCCAAGTCCTTTGCCAACGCCTCCTACCTGGCCCAGCACCTGCGCATCCACCTGGGCGTC
NM_152493.2         TCCAAGTCCTTTGCCAACGCCTCCTACCTGGCCCAGCACCTGCGCATCCACCTGGGCGTC
                    ************************************************************

HsCD00329482        AAGCCCTACCACTGCTCCTACTGTGATAAGTCCTTCCGGCAGCTCTCCCACCTCCAGCAG
XM_005270507.1      AAGCCCTACCACTGCTCCTACTGTGATAAGTCCTTCCGGCAGCTCTCCCACCTCCAGCAG
NM_152493.2         AAGCCCTACCACTGCTCCTACTGTGATAAGTCCTTCCGGCAGCTCTCCCACCTCCAGCAG
                    ************************************************************

HsCD00329482        CACACCAGAATCCACACAGGCGACAGACCCTACAAGTGCCCACATCCTGGCTGCGAGAAG
XM_005270507.1      CACACCAGAATCCACACAGGCGACAGACCCTACAAGTGCCCACATCCTGGCTGCGAGAAG
NM_152493.2         CACACCAGAATCCACACAGGCGACAGACCCTACAAGTGCCCACATCCTGGCTGCGAGAAG
                    ************************************************************

HsCD00329482        GCTTTCACTCAGCTCTCCAACCTCCAGTCTCACCAGCGCCAGCACAACAAGGACAAGCCC
XM_005270507.1      GCTTTCACTCAGCTCTCCAACCTCCAGTCTCACCAGCGCCAGCACAACAAGGACAAGCCC
NM_152493.2         GCTTTCACTCAGCTCTCCAACCTCCAGTCTCACCAGCGCCAGCACAACAAGGACAAGCCC
                    ************************************************************

HsCD00329482        TACAAGTGTCCCAACTGCTACCGGGCCTATTCGGACTCCGCTTCTTTGCAGATCCACCTC
XM_005270507.1      TACAAGTGTCCCAACTGCTACCGGGCCTATTCGGACTCCGCTTCTTTGCAGATCCACCTC
NM_152493.2         TACAAGTGTCCCAACTGCTACCGGGCCTATTCGGACTCCGCTTCTTTGCAGATCCACCTC
                    ************************************************************

HsCD00329482        TCGGCCCACGCCATCAAGCACGCCAAGGCCTACTGCTGCAGCATGTGTGGGCGGGCCTAC
XM_005270507.1      TCGGCCCACGCCATCAAGCACGCCAAGGCCTACTGCTGCAGCATGTGTGGGCGGGCCTAC
NM_152493.2         TCGGCCCACGCCATCAAGCACGCCAAGGCCTACTGCTGCAGCATGTGTGGGCGGGCCTAC
                    ************************************************************

HsCD00329482        ACCTCGGAGACCTACCTGATGAAGCACATGTCCAAACACACGGTGGTGGAGCACCTGGTG
XM_005270507.1      ACCTCGGAGACCTACCTGATGAAGCACATGTCCAAACACACGGTGGTGGAGCACCTGGTG
NM_152493.2         ACCTCGGAGACCTACCTGATGAAGCACATGTCCAAACACACGGTGGTGGAGCACCTGGTG
                    ************************************************************

HsCD00329482        AGCCATCACTCGCCCCAGAGGACGGAGTCCCCCGGCATCCCGGTGCGAATCTCTCTCATC
XM_005270507.1      AGCCATCACTCGCCCCAGAGGACGGAGTCCCCCGGCATCCCGGTGCGAATCTCTCTCATC
NM_152493.2         AGCCATCACTCGCCCCAGAGGACGGAGTCCCCCGGCATCCCGGTGCGAATCTCTCTCATC
                    ************************************************************

HsCD00329482        TTG
XM_005270507.1      TGA
NM_152493.2         TGA
                    * .

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q5T0B9
HPRD : 13394

Clone

Cloning Information : 268371
HIP Master Clone ID : 193233
Original Clone ID : FLH268371.01L

Organism


TAX_ID : 9606
Species Specific ID: 149076

Genome

Chromosome : 1
Map Location : 1p35.1
Ensembl : ENSG00000160094

Reagents

Labome : ZNF362-antibody