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POU2F3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  POU2F3
Gene Name:  POU class 2 homeobox 3
Sequence             
Original Clone ID: FLH268373.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329483

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1311nts         Open reading frame : 1 to 1311
Reference Sequence Alignment

ATGGTGAATCTGGAGTCCATGCACACAGATATCAAGATGAGTGGGGATGTAGCCGATTCC
ACGGATGCTCGCAGCACTCTCAGCCAGGTGGAGCCAGGAAATGATCGAAATGGCCTAGAT
TTCAACAGGCAGATTAAAACCGAAGATCTCAGTGACTCCCTGCAGCAGACCCTCTCCCAT
CGGCCATGCCACCTGAGTCAAGGACCTGCCATGATGTCCGGAAACCAAATGTCTGGGCTA
AATGCCAGCCCATGTCAGGACATGGCTTCCCTCCATCCGCTCCAGCAGCTTGTGCTGGTT
CCCGGCCACTTACAGTCTGTATCCCAGTTCCTGCTATCTCAGACCCAGCCTGGGCAGCAA
GGTCTGCAGCCAAATCTCCTCCCCTTTCCACAGCAACAAAGCGGTCTCCTCCTCCCACAG
ACTGGGCCGGGACTGGCATCCCAGGCATTTGGGCACCCTGGGCTGCCAGGATCCTCTTTA
GAACCCCACCTGGAAGCATCCCAGCATCTCCCAGTGCCCAAGCATCTACCCAGCTCTGGA
GGGGCCGATGAGCCCAGTGACCTCGAGGAGCTGGAGAAGTTTGCCAAGACCTTCAAGCAG
AGGCGCATTAAGCTGGGCTTCACACAGGGAGATGTGGGGCTGGCGATGGGAAAGCTGTAT
GGCAACGACTTCAGCCAGACCACCATCTCACGATTTGAGGCCCTCAACCTGAGCTTCAAG
AACATGTGCAAGCTCAAGCCCCTGCTGGAGAAGTGGCTGAATGATGCAGAGTCCTCTCCG
TCAGACCCCTCAGTGAGCACGCCCAGCTCCTACCCCAGCCTCAGTGAAGTATTTGGTAGG
AAGAGAAAGAAACGGACCAGCATCGAGACCAACATCCGCCTGACTCTGGAGAAGAGGTTT
CAAGATAACCCAAAACCCAGCTCGGAGGAGATCTCCATGATTGCAGAGCAGTTGTCCATG
GAGAAGGAGGTGGTGAGGGTCTGGTTCTGCAACCGACGCCAAAAGGAGAAGCGAATCAAC
TGCCCTGTGGCCACACCCATCAAACCACCTGTCTACAACTCCCGGCTGGTATCTCCCTCA
GGGTCTCTGGGCCCCCTCTCTGTCCCTCCTGTCCACAGTACCATGCCTGGAACAGTAACG
TCATCCTGTTCCCCTGGGAACAACAGCAGGCCTTCATCTCCTGGCTCAGGACTCCACGCC
AGCAGCCCCACTGCATCTCAAAATAACTCCAAAGCAGCAGTGAACTCCGCCTCCAGTTTT
AACTCTTCAGGATCTTGGTACCGATGGAATCATTCCACCTACCTCCACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1396
Start on reference sequence 86
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POU2F3
Symbol Nomenclature : POU2F3
SYNONYM : Epoc-1; OCT-11; OCT11; OTF-11; PLA-1; PLA1; Skn-1a
Designation : POU domain transcription factor OCT11a|POU domain, class 2, transcription factor 3|octamer-binding protein 11|octamer-binding transcription factor 11|transcription factor PLA-1|transcription factor Skn-1
Full Nomenclature : POU class 2 homeobox 3
GENEID : 25833
HGNC : 19864
MIM : 607394
Vega : OTTHUMG00000166140
Target GenBank: BC101649

 

Reference Sequence Alignment

  

HsCD00329483        ------ATGGTGAATCTGGAGTCCATGCACACAGATATCAAGATGAGTGGGGATGTAGCC
NM_014352.3         ------ATGGTGAATCTGGAGTCCATGCACACAGATATCAAGATGAGTGGGGATGTAGCC
NM_001244682.1      ATGGAGTCTCCAAGAACTGCTAAAGGAGGAAGGGATATCAAGATGAGTGGGGATGTAGCC
                          :    .*.:.  *. :... . ..* .***************************

HsCD00329483        GATTCCACGGATGCTCGCAGCACTCTCAGCCAGGTGGAGCCAGGAAATGATCGAAATGGC
NM_014352.3         GATTCCACGGATGCTCGCAGCACTCTCAGCCAGGTGGAGCCAGGAAATGATCGAAATGGC
NM_001244682.1      GATTCCACGGATGCTCGCAGCACTCTCAGCCAGGTGGAGCCAGGAAATGATCGAAATGGC
                    ************************************************************

HsCD00329483        CTAGATTTCAACAGGCAGATTAAAACCGAAGATCTCAGTGACTCCCTGCAGCAGACCCTC
NM_014352.3         CTAGATTTCAACAGGCAGATTAAAACCGAAGATCTCAGTGACTCCCTGCAGCAGACCCTC
NM_001244682.1      CTAGATTTCAACAGGCAGATTAAAACCGAAGATCTCAGTGACTCCCTGCAGCAGACCCTC
                    ************************************************************

HsCD00329483        TCCCATCGGCCATGCCACCTGAGTCAAGGACCTGCCATGATGTCCGGAAACCAAATGTCT
NM_014352.3         TCCCATCGGCCATGCCACCTGAGTCAAGGACCTGCCATGATGTCCGGAAACCAAATGTCT
NM_001244682.1      TCCCATCGGCCATGCCACCTGAGTCAAGGACCTGCCATGATGTCCGGAAACCAAATGTCT
                    ************************************************************

HsCD00329483        GGGCTAAATGCCAGCCCATGTCAGGACATGGCTTCCCTCCATCCGCTCCAGCAGCTTGTG
NM_014352.3         GGGCTAAATGCCAGCCCATGTCAGGACATGGCTTCCCTCCATCCGCTCCAGCAGCTTGTG
NM_001244682.1      GGGCTAAATGCCAGCCCATGTCAGGACATGGCTTCCCTCCATCCGCTCCAGCAGCTTGTG
                    ************************************************************

HsCD00329483        CTGGTTCCCGGCCACTTACAGTCTGTATCCCAGTTCCTGCTATCTCAGACCCAGCCTGGG
NM_014352.3         CTGGTTCCCGGCCACTTACAGTCTGTATCCCAGTTCCTGCTATCTCAGACCCAGCCTGGG
NM_001244682.1      CTGGTTCCCGGCCACTTACAGTCTGTATCCCAGTTCCTGCTATCTCAGACCCAGCCTGGG
                    ************************************************************

HsCD00329483        CAGCAAGGTCTGCAGCCAAATCTCCTCCCCTTTCCACAGCAACAAAGCGGTCTCCTCCTC
NM_014352.3         CAGCAAGGTCTGCAGCCAAATCTCCTCCCCTTTCCACAGCAACAAAGCGGTCTCCTCCTC
NM_001244682.1      CAGCAAGGTCTGCAGCCAAATCTCCTCCCCTTTCCACAGCAACAAAGCGGTCTCCTCCTC
                    ************************************************************

HsCD00329483        CCACAGACTGGGCCGGGACTGGCATCCCAGGCATTTGGGCACCCTGGGCTGCCAGGATCC
NM_014352.3         CCACAGACTGGGCCGGGACTGGCATCCCAGGCATTTGGGCACCCTGGGCTGCCAGGATCC
NM_001244682.1      CCACAGACTGGGCCGGGACTGGCATCCCAGGCATTTGGGCACCCTGGGCTGCCAGGATCC
                    ************************************************************

HsCD00329483        TCTTTAGAACCCCACCTGGAAGCATCCCAGCATCTCCCAGTGCCCAAGCATCTACCCAGC
NM_014352.3         TCTTTAGAACCCCACCTGGAAGCATCCCAGCATCTCCCAGTGCCCAAGCATCTACCCAGC
NM_001244682.1      TCTTTAGAACCCCACCTGGAAGCATCCCAGCATCTCCCAGTGCCCAAGCATCTACCCAGC
                    ************************************************************

HsCD00329483        TCTGGAGGGGCCGATGAGCCCAGTGACCTCGAGGAGCTGGAGAAGTTTGCCAAGACCTTC
NM_014352.3         TCTGGAGGGGCCGATGAGCCCAGTGACCTCGAGGAGCTGGAGAAGTTTGCCAAGACCTTC
NM_001244682.1      TCTGGAGGGGCCGATGAGCCCAGTGACCTCGAGGAGCTGGAGAAGTTTGCCAAGACCTTC
                    ************************************************************

HsCD00329483        AAGCAGAGGCGCATTAAGCTGGGCTTCACACAGGGAGATGTGGGGCTGGCGATGGGAAAG
NM_014352.3         AAGCAGAGGCGCATTAAGCTGGGCTTCACACAGGGAGATGTGGGGCTGGCGATGGGAAAG
NM_001244682.1      AAGCAGAGGCGCATTAAGCTGGGCTTCACACAGGGAGATGTGGGGCTGGCGATGGGAAAG
                    ************************************************************

HsCD00329483        CTGTATGGCAACGACTTCAGCCAGACCACCATCTCACGATTTGAGGCCCTCAACCTGAGC
NM_014352.3         CTGTATGGCAACGACTTCAGCCAGACCACCATCTCACGATTTGAGGCCCTCAACCTGAGC
NM_001244682.1      CTGTATGGCAACGACTTCAGCCAGACCACCATCTCACGATTTGAGGCCCTCAACCTGAGC
                    ************************************************************

HsCD00329483        TTCAAGAACATGTGCAAGCTCAAGCCCCTGCTGGAGAAGTGGCTGAATGATGCAGAGTCC
NM_014352.3         TTCAAGAACATGTGCAAGCTCAAGCCCCTGCTGGAGAAGTGGCTGAATGATGCAGAGTCC
NM_001244682.1      TTCAAGAACATGTGCAAGCTCAAGCCCCTGCTGGAGAAGTGGCTGAATGATGCAGAGTCC
                    ************************************************************

HsCD00329483        TCTCCGTCAGACCCCTCAGTGAGCACGCCCAGCTCCTACCCCAGCCTCAGTGAAGTATTT
NM_014352.3         TCTCCGTCAGACCCCTCAGTGAGCACGCCCAGCTCCTACCCCAGCCTCAGTGAAGTATTT
NM_001244682.1      TCTCCGTCAGACCCCTCAGTGAGCACGCCCAGCTCCTACCCCAGCCTCAGTGAAGTATTT
                    ************************************************************

HsCD00329483        GGTAGGAAGAGAAAGAAACGGACCAGCATCGAGACCAACATCCGCCTGACTCTGGAGAAG
NM_014352.3         GGTAGGAAGAGAAAGAAACGGACCAGCATCGAGACCAACATCCGCCTGACTCTGGAGAAG
NM_001244682.1      GGTAGGAAGAGAAAGAAACGGACCAGCATCGAGACCAACATCCGCCTGACTCTGGAGAAG
                    ************************************************************

HsCD00329483        AGGTTTCAAGATAACCCAAAACCCAGCTCGGAGGAGATCTCCATGATTGCAGAGCAGTTG
NM_014352.3         AGGTTTCAAGATAACCCAAAACCCAGCTCGGAGGAGATCTCCATGATTGCAGAGCAGTTG
NM_001244682.1      AGGTTTCAAGATAACCCAAAACCCAGCTCGGAGGAGATCTCCATGATTGCAGAGCAGTTG
                    ************************************************************

HsCD00329483        TCCATGGAGAAGGAGGTGGTGAGGGTCTGGTTCTGCAACCGACGCCAAAAGGAGAAGCGA
NM_014352.3         TCCATGGAGAAGGAGGTGGTGAGGGTCTGGTTCTGCAACCGACGCCAAAAGGAGAAGCGA
NM_001244682.1      TCCATGGAGAAGGAGGTGGTGAGGGTCTGGTTCTGCAACCGACGCCAAAAGGAGAAGCGA
                    ************************************************************

HsCD00329483        ATCAACTGCCCTGTGGCCACACCCATCAAACCACCTGTCTACAACTCCCGGCTGGTATCT
NM_014352.3         ATCAACTGCCCTGTGGCCACACCCATCAAACCACCTGTCTACAACTCCCGGCTGGTATCT
NM_001244682.1      ATCAACTGCCCTGTGGCCACACCCATCAAACCACCTGTCTACAACTCCCGGCTGGTATCT
                    ************************************************************

HsCD00329483        CCCTCAGGGTCTCTGGGCCCCCTCTCTGTCCCTCCTGTCCACAGTACCATGCCTGGAACA
NM_014352.3         CCCTCAGGGTCTCTGGGCCCCCTCTCTGTCCCTCCTGTCCACAGTACCATGCCTGGAACA
NM_001244682.1      CCCTCAGGGTCTCTGGGCCCCCTCTCTGTCCCTCCTGTCCACAGTACCATGCCTGGAACA
                    ************************************************************

HsCD00329483        GTAACGTCATCCTGTTCCCCTGGGAACAACAGCAGGCCTTCATCTCCTGGCTCAGGACTC
NM_014352.3         GTAACGTCATCCTGTTCCCCTGGGAACAACAGCAGGCCTTCATCTCCTGGCTCAGGACTC
NM_001244682.1      GTAACGTCATCCTGTTCCCCTGGGAACAACAGCAGGCCTTCATCTCCTGGCTCAGGACTC
                    ************************************************************

HsCD00329483        CACGCCAGCAGCCCCACTGCATCTCAAAATAACTCCAAAGCAGCAGTGAACTCCGCCTCC
NM_014352.3         CACGCCAGCAGCCCCACTGCATCTCAAAATAACTCCAAAGCAGCAGTGAACTCCGCCTCC
NM_001244682.1      CACGCCAGCAGCCCCACTGCATCTCAAAATAACTCCAAAGCAGCAGTGAACTCCGCCTCC
                    ************************************************************

HsCD00329483        AGTTTTAACTCTTCAGGATCTTGGTACCGATGGAATCATTCCACCTACCTCCACTTG
NM_014352.3         AGTTTTAACTCTTCAGGATCTTGGTACCGATGGAATCATTCCACCTACCTCCACTGA
NM_001244682.1      AGTTTTAACTCTTCAGGATCTTGGTACCGATGGAATCATTCCACCTACCTCCACTGA
                    ******************************************************* .

Protein

SMART domain : HOX : Homeodomain
SMART domain : POU : Found in Pit-Oct-Unc transcription factors
UniProt : Q9UKI9
HPRD : 09582

Clone

Cloning Information : 268373
HIP Master Clone ID : 193235
Original Clone ID : FLH268373.01L

Organism


TAX_ID : 9606
Species Specific ID: 25833

Genome

Chromosome : 11
Map Location : 11q23.3
Ensembl : ENSG00000137709

Reagents

Labome : POU2F3-antibody