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ZNF555 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF555
Gene Name:  zinc finger protein 555
Sequence             
Original Clone ID: FLH268385.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329495

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1323nts         Open reading frame : 1 to 1323
Reference Sequence Alignment

ATGCATGTGAGAACCAACAATGGAGAGAGACCCTATGTGTGTAAATTATGTGGGAAAACC
TTTCCTCGTACTTCCTCCCTCAATCGGCATGTAAGGATTCACACTGCTGAGAAAACCTAC
GAATGTAAGCAATGTGGGAAAGCCTTTATTGACTTCTCAAGTCTTACTAGTCATCTCAGA
AGTCACACCGGAGAGAAGCCATATAAGTGTAAGGAATGTGGGAAAGCTTTCAGTTATTCC
TCAACGTTTCGAAGACACACAATAACACACACTGGCGAGAAGCCATATAAATGTAAGGAA
TGTGCGGAAGCCTTTAGTTATTCCTCAACTTTTCGAAGACATATGATTTCACACACTGGA
GAGAAGCCACATAAATGTAAAGAATGTGGGGAGGCCTTCAGTTATTCTTCGGCTTTTCGA
AGACACATGATAACACACACTGGAGAGAAACCCTACGAATGCAAACAATGTGGGAAAACC
TTCATTTATCTCCAGTCCTTTCGAAGACATGAAAGGATTCACACTGGAGAGAAACCCTAC
GAATGCAAACAGTGTGGGAAGACCTTCATTTATCCCCAGTCCTTTCGAAGACATGAAAGG
ACTCATGGTGGAGAGAAACCCTATGAATGCAACCAGTGCGGGAAAGCATTCAGTCACCCC
TCCTCCTTTCGAGGACACATGAGGGTGCACACTGGAGAGAAACCCTATGAGTGCAAGCAA
TGTGGGAAAACTTTCAATTGGCCCATATCTTTACGAAAACATATGAGAACACATACTAGA
GAGAAACCCTATGAATGTAAGCAGTGTGGGAAAGCCTTCAGCTTGTCTGCTTGCTTTCGA
GAACATGTGAGAATGCACCCTGAAGACAAATCCTATGAATGCAAGCTATGTGGGAAAGCT
TTCTATTGCCACATATCCTTACAAAAACATATGAGAAGACATACCGCAGAGAAACTCTAT
AAATGCAAGCAGTGTGGGACAGCTTTCAGTTGGCCTGAACTTTTGCAACAACATGTGAGA
ACGCACACTGTAGAGAAGCCCTATGAATGTAAGGAATGTGGGAAGGTCTTCAAATGGCCA
TCATCTTTACCAATACATATGAGACTGCACACTGGAGAGAAACCTTATCAATGTAAGCAT
TGTGGGAAAGCATTCAATTGTTCCTCATCCTTAAGGCGACATGTGAGAATACACACTACA
GAAAAACAGTATAAGTGTAATGTAGGACATCCTCCTGCAAATGAATTCATGTGCAGTGCT
TCAGAAAAGTCACACCAGGAGAGAGATCTGATCAAAGTTGTAAATATGGTGTTGCCTTTA
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1981
Start on reference sequence 659
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF555
Symbol Nomenclature : ZNF555
Full Nomenclature : zinc finger protein 555
GENEID : 148254
HGNC : 28382
Vega : OTTHUMG00000180500
Target GenBank: BC022022

 

Reference Sequence Alignment

  

HsCD00329495        ------------------------------------------------------------
NM_152791.4         ATGGACTCAGTGGTCTTTGAGGATGTGGCTGTGGACTTCACCCTGGAGGAGTGGGCTTTG
NM_001172775.1      ATGGACTCAGTGGTCTTTGAGGATGTGGCTGTGGACTTCACCCTGGAGGAGTGGGCTTTG
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         CTGGATTCTGCTCAGAGGGACCTCTACAGAGATGTGATGCTGGAGACCTTTCAGAACCTG
NM_001172775.1      CTGGATTCTGCTCAGAGGGACCTCTACAGAGATGTGATGCTGGAGACCTTTCAGAACCTG
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         GCCTCAGTAGATGATGAAACTCAATTTAAGGCCAGTGGGTCAGTTTCTCAGCAGGATATT
NM_001172775.1      GCCTCAGTAGATGATGAAACTCAATTTAAGGCCAGTGGGTCAGTTTCTCAGCAGGATATT
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         TATGGAGAGAAAATACCCAAGGAATCTAAAATAGCCACGTTCACCAGAAATGTTTCCTGG
NM_001172775.1      TATGGAGAGAAAATACCCAAGGAATCTAAAATAGCCACGTTCACCAGAAATGTTTCCTGG
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         GCCTCTGTTTTAGGAAAAATTTGGGACAGTCTTAGCATCGAAGATCAAACCACAAACCAG
NM_001172775.1      GCCTCTGTTTTAGGAAAAATTTGGGACAGTCTTAGCATCGAAGATCAAACCACAAACCAG
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         GGGAGAAATCTCAGTAGAAATCATGGGTTGGAGAGACTCTGTGAAAGTAATGATCAATGT
NM_001172775.1      GGGAGAAATCTCAG---AAATCATGGGTTGGAGAGACTCTGTGAAAGTAATGATCAATGT
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         GGAGAAGCCCTCAGCCAGATTCCACATCTTAATCTGTACAAGAAAATTCCACCTGGAGTA
NM_001172775.1      GGAGAAGCCCTCAGCCAGATTCCACATCTTAATCTGTACAAGAAAATTCCACCTGGAGTA
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         AAACAGTATGAATACAACACGTACGGAAAAGTCTTCATGCATCGCCGCACATCCCTCAAG
NM_001172775.1      AAACAGTATGAATACAACACGTACGGAAAAGTCTTCATGCATCGCCGCACATCCCTCAAG
                                                                                

HsCD00329495        ------------------------------------------------------------
NM_152791.4         AGTCCCATCACAGTTCACACTGGACACAAACCATATCAGTGCCAGGAATGTGGGCAGGCC
NM_001172775.1      AGTCCCATCACAGTTCACACTGGACACAAACCATATCAGTGCCAGGAATGTGGGCAGGCC
                                                                                

HsCD00329495        ------------------------ATGCATGTGAGAACCAACAATGGAGAGAGACCCTAT
NM_152791.4         TACAGTTGTCGTTCACACCTAAGAATGCATGTGAGAACCCACAATGGAGAGAGACCCTAT
NM_001172775.1      TACAGTTGTCGTTCACACCTAAGAATGCATGTGAGAACCCACAATGGAGAGAGACCCTAT
                                            ***************.********************

HsCD00329495        GTGTGTAAATTATGTGGGAAAACCTTTCCTCGTACTTCCTCCCTCAATCGGCATGTAAGG
NM_152791.4         GTGTGTAAATTATGTGGGAAAACCTTTCCTCGTACTTCCTCCCTCAATCGGCATGTAAGG
NM_001172775.1      GTGTGTAAATTATGTGGGAAAACCTTTCCTCGTACTTCCTCCCTCAATCGGCATGTAAGG
                    ************************************************************

HsCD00329495        ATTCACACTGCTGAGAAAACCTACGAATGTAAGCAATGTGGGAAAGCCTTTATTGACTTC
NM_152791.4         ATTCACACTGCTGAGAAAACCTACGAATGTAAGCAATGTGGGAAAGCCTTTATTGACTTC
NM_001172775.1      ATTCACACTGCTGAGAAAACCTACGAATGTAAGCAATGTGGGAAAGCCTTTATTGACTTC
                    ************************************************************

HsCD00329495        TCAAGTCTTACTAGTCATCTCAGAAGTCACACCGGAGAGAAGCCATATAAGTGTAAGGAA
NM_152791.4         TCAAGTCTTACTAGTCATCTCAGAAGTCACACCGGAGAGAAGCCATATAAGTGTAAGGAA
NM_001172775.1      TCAAGTCTTACTAGTCATCTCAGAAGTCACACCGGAGAGAAGCCATATAAGTGTAAGGAA
                    ************************************************************

HsCD00329495        TGTGGGAAAGCTTTCAGTTATTCCTCAACGTTTCGAAGACACACAATAACACACACTGGC
NM_152791.4         TGTGGGAAAGCTTTCAGTTATTCCTCAACGTTTCGAAGACACACAATAACACACACTGGC
NM_001172775.1      TGTGGGAAAGCTTTCAGTTATTCCTCAACGTTTCGAAGACACACAATAACACACACTGGC
                    ************************************************************

HsCD00329495        GAGAAGCCATATAAATGTAAGGAATGTGCGGAAGCCTTTAGTTATTCCTCAACTTTTCGA
NM_152791.4         GAGAAGCCATATAAATGTAAGGAATGTGCGGAAGCCTTTAGTTATTCCTCAACTTTTCGA
NM_001172775.1      GAGAAGCCATATAAATGTAAGGAATGTGCGGAAGCCTTTAGTTATTCCTCAACTTTTCGA
                    ************************************************************

HsCD00329495        AGACATATGATTTCACACACTGGAGAGAAGCCACATAAATGTAAAGAATGTGGGGAGGCC
NM_152791.4         AGACATATGATTTCACACACTGGAGAGAAGCCACATAAATGTAAAGAATGTGGGGAGGCC
NM_001172775.1      AGACATATGATTTCACACACTGGAGAGAAGCCACATAAATGTAAAGAATGTGGGGAGGCC
                    ************************************************************

HsCD00329495        TTCAGTTATTCTTCGGCTTTTCGAAGACACATGATAACACACACTGGAGAGAAACCCTAC
NM_152791.4         TTCAGTTATTCTTCGGCTTTTCGAAGACACATGATAACACACACTGGAGAGAAACCCTAC
NM_001172775.1      TTCAGTTATTCTTCGGCTTTTCGAAGACACATGATAACACACACTGGAGAGAAACCCTAC
                    ************************************************************

HsCD00329495        GAATGCAAACAATGTGGGAAAACCTTCATTTATCTCCAGTCCTTTCGAAGACATGAAAGG
NM_152791.4         GAATGCAAACAATGTGGGAAAACCTTCATTTATCTCCAGTCCTTTCGAAGACATGAAAGG
NM_001172775.1      GAATGCAAACAATGTGGGAAAACCTTCATTTATCTCCAGTCCTTTCGAAGACATGAAAGG
                    ************************************************************

HsCD00329495        ATTCACACTGGAGAGAAACCCTACGAATGCAAACAGTGTGGGAAGACCTTCATTTATCCC
NM_152791.4         ATTCACACTGGAGAGAAACCCTACGAATGCAAACAGTGTGGGAAGACCTTCATTTATCCC
NM_001172775.1      ATTCACACTGGAGAGAAACCCTACGAATGCAAACAGTGTGGGAAGACCTTCATTTATCCC
                    ************************************************************

HsCD00329495        CAGTCCTTTCGAAGACATGAAAGGACTCATGGTGGAGAGAAACCCTATGAATGCAACCAG
NM_152791.4         CAGTCCTTTCGAAGACATGAAAGGACTCATGGTGGAGAGAAACCCTATGAATGCAACCAG
NM_001172775.1      CAGTCCTTTCGAAGACATGAAAGGACTCATGGTGGAGAGAAACCCTATGAATGCAACCAG
                    ************************************************************

HsCD00329495        TGCGGGAAAGCATTCAGTCACCCCTCCTCCTTTCGAGGACACATGAGGGTGCACACTGGA
NM_152791.4         TGCGGGAAAGCATTCAGTCACCCCTCCTCCTTTCGAGGACACATGAGGGTGCACACTGGA
NM_001172775.1      TGCGGGAAAGCATTCAGTCACCCCTCCTCCTTTCGAGGACACATGAGGGTGCACACTGGA
                    ************************************************************

HsCD00329495        GAGAAACCCTATGAGTGCAAGCAATGTGGGAAAACTTTCAATTGGCCCATATCTTTACGA
NM_152791.4         GAGAAACCCTATGAGTGCAAGCAATGTGGGAAAACTTTCAATTGGCCCATATCTTTACGA
NM_001172775.1      GAGAAACCCTATGAGTGCAAGCAATGTGGGAAAACTTTCAATTGGCCCATATCTTTACGA
                    ************************************************************

HsCD00329495        AAACATATGAGAACACATACTAGAGAGAAACCCTATGAATGTAAGCAGTGTGGGAAAGCC
NM_152791.4         AAACATATGAGAACACATACTAGAGAGAAACCCTATGAATGTAAGCAGTGTGGGAAAGCC
NM_001172775.1      AAACATATGAGAACACATACTAGAGAGAAACCCTATGAATGTAAGCAGTGTGGGAAAGCC
                    ************************************************************

HsCD00329495        TTCAGCTTGTCTGCTTGCTTTCGAGAACATGTGAGAATGCACCCTGAAGACAAATCCTAT
NM_152791.4         TTCAGCTTGTCTGCTTGCTTTCGAGAACATGTGAGAATGCACCCTGAAGACAAATCCTAT
NM_001172775.1      TTCAGCTTGTCTGCTTGCTTTCGAGAACATGTGAGAATGCACCCTGAAGACAAATCCTAT
                    ************************************************************

HsCD00329495        GAATGCAAGCTATGTGGGAAAGCTTTCTATTGCCACATATCCTTACAAAAACATATGAGA
NM_152791.4         GAATGCAAGCTATGTGGGAAAGCTTTCTATTGCCACATATCCTTACAAAAACATATGAGA
NM_001172775.1      GAATGCAAGCTATGTGGGAAAGCTTTCTATTGCCACATATCCTTACAAAAACATATGAGA
                    ************************************************************

HsCD00329495        AGACATACCGCAGAGAAACTCTATAAATGCAAGCAGTGTGGGACAGCTTTCAGTTGGCCT
NM_152791.4         AGACATACCGCAGAGAAACTCTATAAATGCAAGCAGTGTGGGAAAGCTTTCAGTTGGCCT
NM_001172775.1      AGACATACCGCAGAGAAACTCTATAAATGCAAGCAGTGTGGGAAAGCTTTCAGTTGGCCT
                    *******************************************.****************

HsCD00329495        GAACTTTTGCAACAACATGTGAGAACGCACACTGTAGAGAAGCCCTATGAATGTAAGGAA
NM_152791.4         GAACTTTTGCAACAACATGTGAGAACGCACACTGTAGAGAAGCCCTATGAATGTAAGGAA
NM_001172775.1      GAACTTTTGCAACAACATGTGAGAACGCACACTGTAGAGAAGCCCTATGAATGTAAGGAA
                    ************************************************************

HsCD00329495        TGTGGGAAGGTCTTCAAATGGCCATCATCTTTACCAATACATATGAGACTGCACACTGGA
NM_152791.4         TGTGGGAAGGTCTTCAAATGGCCATCATCTTTACCAATACATATGAGACTGCACACTGGA
NM_001172775.1      TGTGGGAAGGTCTTCAAATGGCCATCATCTTTACCAATACATATGAGACTGCACACTGGA
                    ************************************************************

HsCD00329495        GAGAAACCTTATCAATGTAAGCATTGTGGGAAAGCATTCAATTGTTCCTCATCCTTAAGG
NM_152791.4         GAGAAACCTTATCAATGTAAGCATTGTGGGAAAGCATTCAATTGTTCCTCATCCTTAAGG
NM_001172775.1      GAGAAACCTTATCAATGTAAGCATTGTGGGAAAGCATTCAATTGTTCCTCATCCTTAAGG
                    ************************************************************

HsCD00329495        CGACATGTGAGAATACACACTACAGAAAAACAGTATAAGTGTAATGTAGGACATCCTCCT
NM_152791.4         CGACATGTGAGAATACACACTACAGAAAAACAGTATAAGTGTAATGTAGGACATCCTCCT
NM_001172775.1      CGACATGTGAGAATACACACTACAGAAAAACAGTATAAGTGTAATGTAGGACATCCTCCT
                    ************************************************************

HsCD00329495        GCAAATGAATTCATGTGCAGTGCTTCAGAAAAGTCACACCAGGAGAGAGATCTGATCAAA
NM_152791.4         GCAAATGAATTCATGTGCAGTGCTTCAGAAAAGTCACACCAGGAGAGAGATCTGATCAAA
NM_001172775.1      GCAAATGAATTCATGTGCAGTGCTTCAGAAAAGTCACACCAGGAGAGAGATCTGATCAAA
                    ************************************************************

HsCD00329495        GTTGTAAATATGGTGTTGCCTTTATTG
NM_152791.4         GTTGTAAATATGGTGTTGCCTTTATGA
NM_001172775.1      GTTGTAAATATGGTGTTGCCTTTATGA
                    ************************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8NEP9
HPRD : 15838

Clone

Cloning Information : 268385
HIP Master Clone ID : 194861
Original Clone ID : FLH268385.01L

Organism


TAX_ID : 9606
Species Specific ID: 148254

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000186300

Reagents

Labome : ZNF555-antibody