DNASU Plasmid Repository • 480.965.5697 | Email

ZNF230 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZNF230
Gene Name:  zinc finger protein 230
Sequence             
Original Clone ID: FLH268386.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329496

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1425nts         Open reading frame : 1 to 1425
Reference Sequence Alignment

ATGACCACATTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGTTCTTCACTGAGGAG
GAACTGGGGCTACTGGACCCTGCCCAGAGGAAGCTGTACCAAGACGTGATGCTTGAGAAC
TTCACGAACCTGCTGTCAGTGGGGCATCAACCATTCCACCCTTTCCACTTCCTAAGGGAA
GAAAAGTTTTGGATGATGGAGACAGCAACCCAAAGAGAAGGAAATTCCGGCGGCAAGACT
ATTGCGGAAGCAGGACCACATGAAGACTGCCCTTGCCAGCAAATCTGGGAACAAACTGCA
AGTGACTTAACCCAGTCTCAAGACTCCATCATAAATAATTCTCACTTCTTTGAACAAGGT
GATGTCCCCTCCCAGGTTGAGGCAGGACTATCTATAATTCATACAGGACAGAAACCTTCA
CAGAATGGGAAGTGTAAACAGTCCTTCAGTGATGTTGCCATCTTTGATCCTCCTCAGCAG
TTCCACTCAGGAGAGAAGTCTCATACATGCAATGAGTGTGGAAAAAGCTTCTGTTACATC
TCAGCTCTTCGTATTCACCAGAGAGTTCACTTGAGAGAGAAACTCTCTAAGTGTGACATG
CGTGGTAAGGAATTCAGTCAGAGCTCATGTCTGCAAACTCGTGAGAGAGTCCACACTGGA
GAGAAACCATTCAAATGTGAGCAATGTGGGAAAGGCTTCAGATGTAGAGCGATACTTCAA
GTTCACTGCAAATTACACACAGGAGAGAAACCTTATATTTGTGAGAAATGTGGGAGGGCC
TTCATTCACGATTTCCAGCTTCAGAAACATCAGATAATTCATACTGGGGAGAAGCCGTTC
AAATGTGAAATATGTGGTAAGAGCTTCTGCCTTAGGTCAAGTCTTAATAGGCATTGCATG
GTCCACACAGCAGAGAAACTGTACAAATCTGAGGAGTGTGGAAAAGGCTTCACTGATAGC
CTAGATTTGCATAAGCATCAGATAATTCACACAGGACAGAAACCGTACAATTGTAAAGAA
TGTGGGAAGAGCTTCAGATGGTCCTCATATCTTTTGATCCATCAGCGAATCCACAGTGGA
GAAAAACCATACAGATGTGAGGAGTGTGGGAAGGGCTACATTAGTAAGTCAGGTCTTAAC
TTGCACCAGAGGGTCCATACTGGAGAGAGACCTTATAATTGTAAGGAATGTGGGAAGAGC
TTTAGCCGGGCTTCAAGTATTTTGAATCATAAGAAACTCCACTGCCGGAAAAAACCCTTC
AAATGTGAGGATTGTGGAAAGAGGCTTGTACACCGGTCTTTCTATAAAGACCAACAAGGA
GACCACAATGGAGAAAACTCATCCAAATGTGAGGACTGTGGGAAGCGCTACAAGAGGCGC
TTGAATCTGGATATAATTTTATCATTATTTTTAAATGATATGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1676
Start on reference sequence 252
Mutations:
None
Discrepancy:
a228c; g1304a,C435Y
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF230
Symbol Nomenclature : ZNF230
SYNONYM : FDZF2
Designation : zinc finger protein FDZF2
Full Nomenclature : zinc finger protein 230
GENEID : 7773
HGNC : 13024
Vega : OTTHUMG00000182307
Target GenBank: BC094708

 

Reference Sequence Alignment

  

HsCD00329496      ATGACCACATTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGTTCTTCACTGAGGAG
NM_006300.3       ATGACCACATTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGTTCTTCACTGAGGAG
                  ************************************************************

HsCD00329496      GAACTGGGGCTACTGGACCCTGCCCAGAGGAAGCTGTACCAAGACGTGATGCTTGAGAAC
NM_006300.3       GAACTGGGGCTACTGGACCCTGCCCAGAGGAAGCTGTACCAAGACGTGATGCTTGAGAAC
                  ************************************************************

HsCD00329496      TTCACGAACCTGCTGTCAGTGGGGCATCAACCATTCCACCCTTTCCACTTCCTAAGGGAA
NM_006300.3       TTCACGAACCTGCTGTCAGTGGGGCATCAACCATTCCACCCTTTCCACTTCCTAAGGGAA
                  ************************************************************

HsCD00329496      GAAAAGTTTTGGATGATGGAGACAGCAACCCAAAGAGAAGGAAATTCCGGCGGCAAGACT
NM_006300.3       GAAAAGTTTTGGATGATGGAGACAGCAACCCAAAGAGAAGGAAATTCAGGCGGCAAGACT
                  ***********************************************.************

HsCD00329496      ATTGCGGAAGCAGGACCACATGAAGACTGCCCTTGCCAGCAAATCTGGGAACAAACTGCA
NM_006300.3       ATTGCGGAAGCAGGACCACATGAAGACTGCCCTTGCCAGCAAATCTGGGAACAAACTGCA
                  ************************************************************

HsCD00329496      AGTGACTTAACCCAGTCTCAAGACTCCATCATAAATAATTCTCACTTCTTTGAACAAGGT
NM_006300.3       AGTGACTTAACCCAGTCTCAAGACTCCATCATAAATAATTCTCACTTCTTTGAACAAGGT
                  ************************************************************

HsCD00329496      GATGTCCCCTCCCAGGTTGAGGCAGGACTATCTATAATTCATACAGGACAGAAACCTTCA
NM_006300.3       GATGTCCCCTCCCAGGTTGAGGCAGGACTATCTATAATTCATACAGGACAGAAACCTTCA
                  ************************************************************

HsCD00329496      CAGAATGGGAAGTGTAAACAGTCCTTCAGTGATGTTGCCATCTTTGATCCTCCTCAGCAG
NM_006300.3       CAGAATGGGAAGTGTAAACAGTCCTTCAGTGATGTTGCCATCTTTGATCCTCCTCAGCAG
                  ************************************************************

HsCD00329496      TTCCACTCAGGAGAGAAGTCTCATACATGCAATGAGTGTGGAAAAAGCTTCTGTTACATC
NM_006300.3       TTCCACTCAGGAGAGAAGTCTCATACATGCAATGAGTGTGGAAAAAGCTTCTGTTACATC
                  ************************************************************

HsCD00329496      TCAGCTCTTCGTATTCACCAGAGAGTTCACTTGAGAGAGAAACTCTCTAAGTGTGACATG
NM_006300.3       TCAGCTCTTCGTATTCACCAGAGAGTTCACTTGAGAGAGAAACTCTCTAAGTGTGACATG
                  ************************************************************

HsCD00329496      CGTGGTAAGGAATTCAGTCAGAGCTCATGTCTGCAAACTCGTGAGAGAGTCCACACTGGA
NM_006300.3       CGTGGTAAGGAATTCAGTCAGAGCTCATGTCTGCAAACTCGTGAGAGAGTCCACACTGGA
                  ************************************************************

HsCD00329496      GAGAAACCATTCAAATGTGAGCAATGTGGGAAAGGCTTCAGATGTAGAGCGATACTTCAA
NM_006300.3       GAGAAACCATTCAAATGTGAGCAATGTGGGAAAGGCTTCAGATGTAGAGCGATACTTCAA
                  ************************************************************

HsCD00329496      GTTCACTGCAAATTACACACAGGAGAGAAACCTTATATTTGTGAGAAATGTGGGAGGGCC
NM_006300.3       GTTCACTGCAAATTACACACAGGAGAGAAACCTTATATTTGTGAGAAATGTGGGAGGGCC
                  ************************************************************

HsCD00329496      TTCATTCACGATTTCCAGCTTCAGAAACATCAGATAATTCATACTGGGGAGAAGCCGTTC
NM_006300.3       TTCATTCACGATTTCCAGCTTCAGAAACATCAGATAATTCATACTGGGGAGAAGCCGTTC
                  ************************************************************

HsCD00329496      AAATGTGAAATATGTGGTAAGAGCTTCTGCCTTAGGTCAAGTCTTAATAGGCATTGCATG
NM_006300.3       AAATGTGAAATATGTGGTAAGAGCTTCTGCCTTAGGTCAAGTCTTAATAGGCATTGCATG
                  ************************************************************

HsCD00329496      GTCCACACAGCAGAGAAACTGTACAAATCTGAGGAGTGTGGAAAAGGCTTCACTGATAGC
NM_006300.3       GTCCACACAGCAGAGAAACTGTACAAATCTGAGGAGTGTGGAAAAGGCTTCACTGATAGC
                  ************************************************************

HsCD00329496      CTAGATTTGCATAAGCATCAGATAATTCACACAGGACAGAAACCGTACAATTGTAAAGAA
NM_006300.3       CTAGATTTGCATAAGCATCAGATAATTCACACAGGACAGAAACCGTACAATTGTAAAGAA
                  ************************************************************

HsCD00329496      TGTGGGAAGAGCTTCAGATGGTCCTCATATCTTTTGATCCATCAGCGAATCCACAGTGGA
NM_006300.3       TGTGGGAAGAGCTTCAGATGGTCCTCATATCTTTTGATCCATCAGCGAATCCACAGTGGA
                  ************************************************************

HsCD00329496      GAAAAACCATACAGATGTGAGGAGTGTGGGAAGGGCTACATTAGTAAGTCAGGTCTTAAC
NM_006300.3       GAAAAACCATACAGATGTGAGGAGTGTGGGAAGGGCTACATTAGTAAGTCAGGTCTTAAC
                  ************************************************************

HsCD00329496      TTGCACCAGAGGGTCCATACTGGAGAGAGACCTTATAATTGTAAGGAATGTGGGAAGAGC
NM_006300.3       TTGCACCAGAGGGTCCATACTGGAGAGAGACCTTATAATTGTAAGGAATGTGGGAAGAGC
                  ************************************************************

HsCD00329496      TTTAGCCGGGCTTCAAGTATTTTGAATCATAAGAAACTCCACTGCCGGAAAAAACCCTTC
NM_006300.3       TTTAGCCGGGCTTCAAGTATTTTGAATCATAAGAAACTCCACTGCCGGAAAAAACCCTTC
                  ************************************************************

HsCD00329496      AAATGTGAGGATTGTGGAAAGAGGCTTGTACACCGGTCTTTCTATAAAGACCAACAAGGA
NM_006300.3       AAATGTGAGGATTGTGGAAAGAGGCTTGTACACCGGTCTTTCTGTAAAGACCAACAAGGA
                  *******************************************.****************

HsCD00329496      GACCACAATGGAGAAAACTCATCCAAATGTGAGGACTGTGGGAAGCGCTACAAGAGGCGC
NM_006300.3       GACCACAATGGAGAAAACTCATCCAAATGTGAGGACTGTGGGAAGCGCTACAAGAGGCGC
                  ************************************************************

HsCD00329496      TTGAATCTGGATATAATTTTATCATTATTTTTAAATGATATGTTG
NM_006300.3       TTGAATCTGGATATAATTTTATCATTATTTTTAAATGATATGTAA
                  *******************************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9UIE0
HPRD : 15754

Clone

Cloning Information : 268386
HIP Master Clone ID : 194863
Original Clone ID : FLH268386.01L

Organism


TAX_ID : 9606
Species Specific ID: 7773

Genome

Chromosome : 19
Map Location : 19q13.31
Ensembl : ENSG00000159882

Reagents

Labome : ZNF230-antibody