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SCAND1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SCAND1
Gene Name:  SCAN domain containing 1
Sequence             
Original Clone ID: FLH268387.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329497

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 540nts         Open reading frame : 1 to 540
Reference Sequence Alignment

ATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGGGAGTCCCGCGGCGGTGCCACCGGAG
AAACTGGAAGGAGCCGGTTCGAGCTCAGCCCCTGAGCGTAACTGTGTGGGCTCCTCGCTG
CCAGAGGCCTCACCGCCTGCCCCTGAGCCTTCCAGTCCCAACGCCGCGGTCCCTGAAGCC
ATCCCTACGCCCCGAGCTGCGGCCTCCGCGGCCCTGGAGCTGCCTCTCGGGCCCGCACCC
GTGAGCGTAGCGCCTCAGGCCGAAGCTGAAGCGCGCTCCACACCAGGCCCCGCCGGCTCT
AGACTCGGTCCCGAGACGTTCCGCCAGCGTTTCCGGCAGTTCCGCTACCAGGATGCGGCG
GGTCCCCGGGAGGCTTTCCGGCAGCTGCGGGAGCTGTCCCGCCAGTGGCTGCGGCCTGAC
ATCCGCACCAAGGAGCAGATCGTGGAGATGCTGGTGCAAGAGCAGCTGCTCGCCATCCTG
CCCGAGGCGGCTCGGGCCCGGCGGATCCGCCGCCGCACGGATGTGCGCATCACTGGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1611
Start on reference sequence 1072
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SCAND1
Symbol Nomenclature : SCAND1
SYNONYM : RAZ1; SDP1
Designation : SCAN domain-containing 1|SCAN domain-containing protein 1|SCAN-related protein RAZ1
Full Nomenclature : SCAN domain containing 1
GENEID : 51282
HGNC : 10566
MIM : 610416
Target GenBank: BC041022

 

Reference Sequence Alignment

  

HsCD00329497      ------------------------------------------------------------
NM_016558.3       ------------------------------------------------------------
NM_033630.2       ATGCGACTCGAGAACGCTAGAGGGCGCCGCTTCCGGGAGCCCGCAAGCGGCTTCCGGGTG
                                                                              

HsCD00329497      ------------------------------------------------------------
NM_016558.3       ------------------------------------------------------------
NM_033630.2       CTCGCGCGCCGACCTGGACGCAGAGAAGCCAGAGACTTTCGCTTCCGGCTGCCGCAGGCG
                                                                              

HsCD00329497      ------------------------------------------------------------
NM_016558.3       ------------------------------------------------------------
NM_033630.2       CTTCGCTGGTGCAGACGCAGTGCTGAGCACACAGCTACCGGACAAAGAGTGACGCCCGGA
                                                                              

HsCD00329497      ---------ATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGGGAGTCCCGCGGCGGTG
NM_016558.3       ---------ATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGGGAGTCCCGCGGCGGTG
NM_033630.2       GCTGGAGTTATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGGGAGTCCCGCGGCGGTG
                           ***************************************************

HsCD00329497      CCACCGGAGAAACTGGAAGGAGCCGGTTCGAGCTCAGCCCCTGAGCGTAACTGTGTGGGC
NM_016558.3       CCACCGGAGAAACTGGAAGGAGCCGGTTCGAGCTCAGCCCCTGAGCGTAACTGTGTGGGC
NM_033630.2       CCACCGGAGAAACTGGAAGGAGCCGGTTCGAGCTCAGCCCCTGAGCGTAACTGTGTGGGC
                  ************************************************************

HsCD00329497      TCCTCGCTGCCAGAGGCCTCACCGCCTGCCCCTGAGCCTTCCAGTCCCAACGCCGCGGTC
NM_016558.3       TCCTCGCTGCCAGAGGCCTCACCGCCTGCCCCTGAGCCTTCCAGTCCCAACGCCGCGGTC
NM_033630.2       TCCTCGCTGCCAGAGGCCTCACCGCCTGCCCCTGAGCCTTCCAGTCCCAACGCCGCGGTC
                  ************************************************************

HsCD00329497      CCTGAAGCCATCCCTACGCCCCGAGCTGCGGCCTCCGCGGCCCTGGAGCTGCCTCTCGGG
NM_016558.3       CCTGAAGCCATCCCTACGCCCCGAGCTGCGGCCTCCGCGGCCCTGGAGCTGCCTCTCGGG
NM_033630.2       CCTGAAGCCATCCCTACGCCCCGAGCTGCGGCCTCCGCGGCCCTGGAGCTGCCTCTCGGG
                  ************************************************************

HsCD00329497      CCCGCACCCGTGAGCGTAGCGCCTCAGGCCGAAGCTGAAGCGCGCTCCACACCAGGCCCC
NM_016558.3       CCCGCACCCGTGAGCGTAGCGCCTCAGGCCGAAGCTGAAGCGCGCTCCACACCAGGCCCC
NM_033630.2       CCCGCACCCGTGAGCGTAGCGCCTCAGGCCGAAGCTGAAGCGCGCTCCACACCAGGCCCC
                  ************************************************************

HsCD00329497      GCCGGCTCTAGACTCGGTCCCGAGACGTTCCGCCAGCGTTTCCGGCAGTTCCGCTACCAG
NM_016558.3       GCCGGCTCTAGACTCGGTCCCGAGACGTTCCGCCAGCGTTTCCGGCAGTTCCGCTACCAG
NM_033630.2       GCCGGCTCTAGACTCGGTCCCGAGACGTTCCGCCAGCGTTTCCGGCAGTTCCGCTACCAG
                  ************************************************************

HsCD00329497      GATGCGGCGGGTCCCCGGGAGGCTTTCCGGCAGCTGCGGGAGCTGTCCCGCCAGTGGCTG
NM_016558.3       GATGCGGCGGGTCCCCGGGAGGCTTTCCGGCAGCTGCGGGAGCTGTCCCGCCAGTGGCTG
NM_033630.2       GATGCGGCGGGTCCCCGGGAGGCTTTCCGGCAGCTGCGGGAGCTGTCCCGCCAGTGGCTG
                  ************************************************************

HsCD00329497      CGGCCTGACATCCGCACCAAGGAGCAGATCGTGGAGATGCTGGTGCAAGAGCAGCTGCTC
NM_016558.3       CGGCCTGACATCCGCACCAAGGAGCAGATCGTGGAGATGCTGGTGCAAGAGCAGCTGCTC
NM_033630.2       CGGCCTGACATCCGCACCAAGGAGCAGATCGTGGAGATGCTGGTGCAAGAGCAGCTGCTC
                  ************************************************************

HsCD00329497      GCCATCCTGCCCGAGGCGGCTCGGGCCCGGCGGATCCGCCGCCGCACGGATGTGCGCATC
NM_016558.3       GCCATCCTGCCCGAGGCGGCTCGGGCCCGGCGGATCCGCCGCCGCACGGATGTGCGCATC
NM_033630.2       GCCATCCTGCCCGAGGCGGCTCGGGCCCGGCGGATCCGCCGCCGCACGGATGTGCGCATC
                  ************************************************************

HsCD00329497      ACTGGCTTG
NM_016558.3       ACTGGCTGA
NM_033630.2       ACTGGCTGA
                  ******* .

Protein

SMART domain : SCAN : leucine rich region
UniProt : Q9NZG6
UniProt : E1P5S6
UniProt : P57086
HPRD : 15298

Clone

Cloning Information : 268387
HIP Master Clone ID : 194864
Original Clone ID : FLH268387.01L

Organism


TAX_ID : 9606
Species Specific ID: 51282

Genome

Chromosome : 20
Map Location : 20q11.1-q11.23

Reagents

Labome : SCAND1-antibody