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YAF2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  YAF2
Gene Name:  YY1 associated factor 2
Sequence             
Original Clone ID: FLH268392.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329502

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 615nts         Open reading frame : 1 to 615
Reference Sequence Alignment

ATGGGAGACAAGAAGAGCCCCACCAGGCCGAAGCGGCAGCCGAAGCCGTCCTCGGATGAG
GGTTACTGGGACTGTAGCGTCTGCACCTTCCGGAACAGCGCCGAGGCCTTCAAGTGCATG
ATGTGCGATGTGCGGAAGGGCACCTCCACCCGATCCACATTGTTTGAAGTTATTGTGAGT
GCCTCGAGGACCAAGGAACCGTTGAAATTTCCAATTTCAGGAAGGAAACCTCGACCTGTC
TCCCAGTTGGTTGCACAGCAGGTTACTCAGCAGTTTGTGCCTCCTACACAGTCAAAGAAA
GAGAAAAAAGATAAAGTAGAAAAAGAAAAAAGTGAAAAGGAAACAACTAGCAAAAAGAAT
AGCCATAAGAAAACCAGGCCAAGATTGAAAAATGTGGATCGGAGTAGTGCTCAGCATTTG
GAAGTTACTGTTGGAGATCTGACAGTCATTATTACAGACTTTAAGGAGAAAACAAAGTCA
CCGCCTGCATCTAGTGCTGCCTCTGCAGATCAACACAGTCAAAGCGGCTCTAGCTCTGAT
AACACAGAGAGAGGAATGTCCAGGTCATCTTCACCCAGAGGAGAAGCCTCATCATTGAAT
GGAGAATCTCATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 671
Start on reference sequence 57
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : YAF2
Symbol Nomenclature : YAF2
Designation : YY1-associated factor 2
Full Nomenclature : YY1 associated factor 2
GENEID : 10138
HGNC : 17363
MIM : 607534
Target GenBank: BC037777

 

Reference Sequence Alignment

  

HsCD00329502        ATGGGAGACAAGAAGAGCCCCACCAGGCCGAAGCGGCAGCCGAAGCCGTCCTCGGATGAG
NM_001190979.1      ATGGGAGACAAGAAGAGCCCCACCAGGCCGAAGCGGCAGCCGAAGCCGTCCTCGGATGAG
                    ************************************************************

HsCD00329502        GGTTACTGGGACTGTAGCGTCTGCACCTTCCGGAACAGCGCCGAGGCCTTCAAGTGCATG
NM_001190979.1      GGTTACTGGGACTGTAGCGTCTGCACCTTCCGGAACAGCGCCGAGGCCTTCAAGTGCATG
                    ************************************************************

HsCD00329502        ATGTGCGATGTGCGGAAGGGCACCTCCACCCGATCCACATTGTTTGAAGTTATTGTGAGT
NM_001190979.1      ATGTGCGATGTGCGGAAGGGCACCTCCACCCGATCCACATTGTTTGAAGTTATTGTGAGT
                    ************************************************************

HsCD00329502        GCCTCGAGGACCAAGGAACCGTTGAAATTTCCAATTTCAGGAAGGAAACCTCGACCTGTC
NM_001190979.1      GCCTCGAGGACCAAGGAACCGTTGAAATTTCCAATTTCAGGAAGGAAACCTCGACCTGTC
                    ************************************************************

HsCD00329502        TCCCAGTTGGTTGCACAGCAGGTTACTCAGCAGTTTGTGCCTCCTACACAGTCAAAGAAA
NM_001190979.1      TCCCAGTTGGTTGCACAGCAGGTTACTCAGCAGTTTGTGCCTCCTACACAGTCAAAGAAA
                    ************************************************************

HsCD00329502        GAGAAAAAAGATAAAGTAGAAAAAGAAAAAAGTGAAAAGGAAACAACTAGCAAAAAGAAT
NM_001190979.1      GAGAAAAAAGATAAAGTAGAAAAAGAAAAAAGTGAAAAGGAAACAACTAGCAAAAAGAAT
                    ************************************************************

HsCD00329502        AGCCATAAGAAAACCAGGCCAAGATTGAAAAATGTGGATCGGAGTAGTGCTCAGCATTTG
NM_001190979.1      AGCCATAAGAAAACCAGGCCAAGATTGAAAAATGTGGATCGGAGTAGTGCTCAGCATTTG
                    ************************************************************

HsCD00329502        GAAGTTACTGTTGGAGATCTGACAGTCATTATTACAGACTTTAAGGAGAAAACAAAGTCA
NM_001190979.1      GAAGTTACTGTTGGAGATCTGACAGTCATTATTACAGACTTTAAGGAGAAAACAAAGTCA
                    ************************************************************

HsCD00329502        CCGCCTGCATCTAGTGCTGCCTCTGCAGATCAACACAGTCAAAGCGGCTCTAGCTCTGAT
NM_001190979.1      CCGCCTGCATCTAGTGCTGCTTCTGCAGATCAACACAGTCAAAGCGGCTCTAGCTCTGAT
                    ******************** ***************************************

HsCD00329502        AACACAGAGAGAGGAATGTCCAGGTCATCTTCACCCAGAGGAGAAGCCTCATCATTGAAT
NM_001190979.1      AACACAGAGAGAGGAATGTCCAGGTCATCTTCACCCAGAGGAGAAGCCTCATCATTGAAT
                    ************************************************************

HsCD00329502        GGAGAATCTCATTTG
NM_001190979.1      GGAGAATCTCATTAA
                    *************:.

Protein

SMART domain : ZnF_RBZ : Zinc finger domain
UniProt : B4DFU3
UniProt : Q8IY57
HPRD : 09606

Clone

Cloning Information : 268392
HIP Master Clone ID : 194872
Original Clone ID : FLH268392.01L

Organism


TAX_ID : 9606
Species Specific ID: 10138

Genome

Chromosome : 12
Map Location : 12q12
Ensembl : ENSG00000015153

Reagents

Labome : YAF2-antibody