DNASU Plasmid Repository • 480.965.5697 | Email

LOC91614 (H. sapiens ) in pLenti6.2/V5-DEST

TOP

Explanation of Terms


Gene: Gene Symbol:  DEPDC7
Gene Name:  DEP domain containing 7
Sequence             
Original Clone ID: FLH268395.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329505

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1536nts         Open reading frame : 1 to 1536
Reference Sequence Alignment

ATGGCCACCGTGCAGGAGAAGGCTGCTGCGCTGAACCTCTCGGCTCTCCACAGCCCCGCG
CACAGGCCTCCGGGTTTCAGTGTAGCTCAGAAGCCATTTGGAGCCACGTATGTATGGAGC
AGCATCATAAACACTCTTCAAACACAAGTGGAAGTGAAAAAACGAAGGCACCGTTTAAAA
CGACATAATGACTGCTTTGTTGGTTCAGAAGCTGTGGATGTCATTTTTTCTCACCTAATT
CAGAATAAGTATTTTGGTGATGTAGATATTCCTCGAGCCAAAGTGGTGAGAGTGTGTCAA
GCGCTTATGGACTACAAAGTATTTGAAGCAGTTCCAACCAAAGTCTTTGGAAAAGACAAA
AAACCTACATTTGAAGATAGTAGTTGCAGCCTTTATAGATTCACCACAATACCTAACCAA
GACAGTCAGTTAGGCAAAGAGAACAAACTATATTCACCTGCCAGGTATGCAGATGCATTA
TTTAAGTCATCCGATATCAGATCAGCCAGTTTAGAGGACCTGTGGGAAAATCTGAGTTTA
AAGCCAGCCAACTCCCCTCATGTAAATATCTCTGCAACCTTGTCTCCACAAGTTATTAAT
GAAGTGTGGCAAGAAGAAACAATTGGGCGTCTACTACAACTTGTAGACCTTCCACTTCTT
GACTCCTTACTGAAACAGCAAGAGGCTGTACCTAAAATTCCTCAACCTAAGAGGCAGTCC
ACCATGGTCAACAGCAGTAACTATCTGGATCGAGGGATTCTCAAGGCTTATAGTGACTCT
CAGGAAGATGAGTGGCTCTCGGCAGCAATTGACTGTTTAGAATACCTTCCAGACCAAATG
GTGGTGGAAATAAGCAGAAGCTTTCCTGAGCAACCAGACCGAACAGACTTAGTGAAAGAA
CTTCTGTTTGATGCCATTGGCAGATATTACAGTAGTAGGGAACCTCTGTTAAATCACTTA
TCTGACGTTCATAATGGAATTGCAGAACTCTTAGTGAATGGGAAGACGGAAATAGCTTTA
GAAGCTACCCAGCTCCTTCTAAAGCTTTTAGATTTCCAAAATAGAGAAGAATTTAGAAGA
CTACTGTATTTCATGGCTGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAAGT
GACAACCGAATGGTTGTGAAAAGGATATTCTCAAAAGCTATTGTTGACAATAAAAATTTA
TCCAAAGGCAAAACAGATCTTCTGGTACTCTTTTTAATGGATCATCAGAAAGATGTTTTT
AAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTATGGCCATACAGAACGGA
AGAGATCCAAATAGAGATGCAGGATATATTTATTGCCAGAGAATTGATCAACGTGACTAT
TCCAACAATATAGAGAAGACAACCAAAGATGAGCTGTTGAATTTACTAAAAACTCTTGAT
GAGGATTCAAAACTTTCTGCCAAAGAGAAGAAAAAATTGCTAGGTCAATTCTATAAGTGT
CACCCAGACATCTTTATTGAGCATTTTGGAGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1628
Start on reference sequence 93
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DEPDC7
Symbol Nomenclature : DEPDC7
SYNONYM : TR2; dJ85M6.4
Designation : DEP domain-containing protein 7|dJ85M6.4 (novel 58.3 KDA protein)|novel 58.3 KDA protein
Full Nomenclature : DEP domain containing 7
GENEID : 91614
HGNC : 29899
MIM : 612294
Vega : OTTHUMG00000166242
Target GenBank: BC030970

 

Reference Sequence Alignment

  

HsCD00329505        ATGGCCACCGTGCAGGAGAAGGCTGCTGCGCTGAACCTCTCGGCTCTCCACAGCCCCGCG
NM_001077242.1      ATGGCCACCGTGCAGGAGAAGGCTGCTGCGCTGAACCTCTCGGCTCTCCACAGCCCCGCG
NM_139160.2         ---------------------------ATGAGAGGTTTATGTGAGTTCTACTGGCAGGAA
                                               . *. ...  *.*  *.  ** **:* *. *..

HsCD00329505        CACAGGCCTCCGGGTTTCAGTGTAGCTCAGAAGCCATTTGGAGCCACGTATGTATGGAGC
NM_001077242.1      CACAGGCCTCCGGGTTTCAGTGTAGCTCAGAAGCCATTTGGAGCCACGTATGTATGGAGC
NM_139160.2         TTTGGCATAAAAGGTTTCAGTGTAGCTCAGAAGCCATTTGGAGCCACGTATGTATGGAGC
                     : .* . :...************************************************

HsCD00329505        AGCATCATAAACACTCTTCAAACACAAGTGGAAGTGAAAAAACGAAGGCACCGTTTAAAA
NM_001077242.1      AGCATCATAAACACTCTTCAAACACAAGTGGAAGTGAAAAAACGAAGGCACCGTTTAAAA
NM_139160.2         AGCATCATAAACACTCTTCAAACACAAGTGGAAGTGAAAAAACGAAGGCACCGTTTAAAA
                    ************************************************************

HsCD00329505        CGACATAATGACTGCTTTGTTGGTTCAGAAGCTGTGGATGTCATTTTTTCTCACCTAATT
NM_001077242.1      CGACATAATGACTGCTTTGTTGGTTCAGAAGCTGTGGATGTCATTTTTTCTCACCTAATT
NM_139160.2         CGACATAATGACTGCTTTGTTGGTTCAGAAGCTGTGGATGTCATTTTTTCTCACCTAATT
                    ************************************************************

HsCD00329505        CAGAATAAGTATTTTGGTGATGTAGATATTCCTCGAGCCAAAGTGGTGAGAGTGTGTCAA
NM_001077242.1      CAGAATAAGTATTTTGGTGATGTAGATATTCCTCGAGCCAAAGTGGTGAGAGTGTGTCAA
NM_139160.2         CAGAATAAGTATTTTGGTGATGTAGATATTCCTCGAGCCAAAGTGGTGAGAGTGTGTCAA
                    ************************************************************

HsCD00329505        GCGCTTATGGACTACAAAGTATTTGAAGCAGTTCCAACCAAAGTCTTTGGAAAAGACAAA
NM_001077242.1      GCGCTTATGGACTACAAAGTATTTGAAGCAGTTCCAACCAAAGTCTTTGGAAAAGACAAA
NM_139160.2         GCGCTTATGGACTACAAAGTATTTGAAGCAGTTCCAACCAAAGTCTTTGGAAAAGACAAA
                    ************************************************************

HsCD00329505        AAACCTACATTTGAAGATAGTAGTTGCAGCCTTTATAGATTCACCACAATACCTAACCAA
NM_001077242.1      AAACCTACATTTGAAGATAGTAGTTGCAGCCTTTATAGATTCACCACAATACCTAACCAA
NM_139160.2         AAACCTACATTTGAAGATAGTAGTTGCAGCCTTTATAGATTCACCACAATACCTAACCAA
                    ************************************************************

HsCD00329505        GACAGTCAGTTAGGCAAAGAGAACAAACTATATTCACCTGCCAGGTATGCAGATGCATTA
NM_001077242.1      GACAGTCAGTTAGGCAAAGAGAACAAACTATATTCACCTGCCAGGTATGCAGATGCATTA
NM_139160.2         GACAGTCAGTTAGGCAAAGAGAACAAACTATATTCACCTGCCAGGTATGCAGATGCATTA
                    ************************************************************

HsCD00329505        TTTAAGTCATCCGATATCAGATCAGCCAGTTTAGAGGACCTGTGGGAAAATCTGAGTTTA
NM_001077242.1      TTTAAGTCATCCGATATCAGATCAGCCAGTTTAGAGGACCTGTGGGAAAATCTGAGTTTA
NM_139160.2         TTTAAGTCATCCGATATCAGATCAGCCAGTTTAGAGGACCTGTGGGAAAATCTGAGTTTA
                    ************************************************************

HsCD00329505        AAGCCAGCCAACTCCCCTCATGTAAATATCTCTGCAACCTTGTCTCCACAAGTTATTAAT
NM_001077242.1      AAGCCTGCCAACTCCCCTCATGTAAATATCTCTGCAACCTTGTCTCCACAAGTTATTAAT
NM_139160.2         AAGCCTGCCAACTCCCCTCATGTAAATATCTCTGCAACCTTGTCTCCACAAGTTATTAAT
                    *****:******************************************************

HsCD00329505        GAAGTGTGGCAAGAAGAAACAATTGGGCGTCTACTACAACTTGTAGACCTTCCACTTCTT
NM_001077242.1      GAAGTGTGGCAAGAAGAAACAATTGGGCGTCTACTACAACTTGTAGACCTTCCACTTCTT
NM_139160.2         GAAGTGTGGCAAGAAGAAACAATTGGGCGTCTACTACAACTTGTAGACCTTCCACTTCTT
                    ************************************************************

HsCD00329505        GACTCCTTACTGAAACAGCAAGAGGCTGTACCTAAAATTCCTCAACCTAAGAGGCAGTCC
NM_001077242.1      GACTCCTTACTGAAACAGCAAGAGGCTGTACCTAAAATTCCTCAACCTAAGAGGCAGTCC
NM_139160.2         GACTCCTTACTGAAACAGCAAGAGGCTGTACCTAAAATTCCTCAACCTAAGAGGCAGTCC
                    ************************************************************

HsCD00329505        ACCATGGTCAACAGCAGTAACTATCTGGATCGAGGGATTCTCAAGGCTTATAGTGACTCT
NM_001077242.1      ACCATGGTCAACAGCAGTAACTATCTGGATCGAGGGATTCTCAAGGCTTATAGTGACTCT
NM_139160.2         ACCATGGTCAACAGCAGTAACTATCTGGATCGAGGGATTCTCAAGGCTTATAGTGACTCT
                    ************************************************************

HsCD00329505        CAGGAAGATGAGTGGCTCTCGGCAGCAATTGACTGTTTAGAATACCTTCCAGACCAAATG
NM_001077242.1      CAGGAAGATGAGTGGCTCTCGGCAGCAATTGACTGTTTAGAATACCTTCCAGACCAAATG
NM_139160.2         CAGGAAGATGAGTGGCTCTCGGCAGCAATTGACTGTTTAGAATACCTTCCAGACCAAATG
                    ************************************************************

HsCD00329505        GTGGTGGAAATAAGCAGAAGCTTTCCTGAGCAACCAGACCGAACAGACTTAGTGAAAGAA
NM_001077242.1      GTGGTGGAAATAAGCAGAAGCTTTCCTGAGCAACCAGACCGAACAGACTTAGTGAAAGAA
NM_139160.2         GTGGTGGAAATAAGCAGAAGCTTTCCTGAGCAACCAGACCGAACAGACTTAGTGAAAGAA
                    ************************************************************

HsCD00329505        CTTCTGTTTGATGCCATTGGCAGATATTACAGTAGTAGGGAACCTCTGTTAAATCACTTA
NM_001077242.1      CTTCTGTTTGATGCCATTGGCAGATATTACAGTAGTAGGGAACCTCTGTTAAATCACTTA
NM_139160.2         CTTCTGTTTGATGCCATTGGCAGATATTACAGTAGTAGGGAACCTCTGTTAAATCACTTA
                    ************************************************************

HsCD00329505        TCTGACGTTCATAATGGAATTGCAGAACTCTTAGTGAATGGGAAGACGGAAATAGCTTTA
NM_001077242.1      TCTGACGTTCATAATGGAATTGCAGAACTCTTAGTGAATGGGAAGACGGAAATAGCTTTA
NM_139160.2         TCTGACGTTCATAATGGAATTGCAGAACTCTTAGTGAATGGGAAGACGGAAATAGCTTTA
                    ************************************************************

HsCD00329505        GAAGCTACCCAGCTCCTTCTAAAGCTTTTAGATTTCCAAAATAGAGAAGAATTTAGAAGA
NM_001077242.1      GAAGCTACCCAGCTCCTTCTAAAGCTTTTAGATTTCCAAAATAGAGAAGAATTTAGAAGA
NM_139160.2         GAAGCTACCCAGCTCCTTCTAAAGCTTTTAGATTTCCAAAATAGAGAAGAATTTAGAAGA
                    ************************************************************

HsCD00329505        CTACTGTATTTCATGGCTGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAAGT
NM_001077242.1      CTACTGTATTTCATGGCTGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAAGT
NM_139160.2         CTACTGTATTTCATGGCTGTTGCAGCAAATCCTTCTGAGTTTAAATTACAGAAAGAAAGT
                    ************************************************************

HsCD00329505        GACAACCGAATGGTTGTGAAAAGGATATTCTCAAAAGCTATTGTTGACAATAAAAATTTA
NM_001077242.1      GACAACCGAATGGTTGTGAAAAGGATATTCTCAAAAGCTATTGTTGACAATAAAAATTTA
NM_139160.2         GACAACCGAATGGTTGTGAAAAGGATATTCTCAAAAGCTATTGTTGACAATAAAAATTTA
                    ************************************************************

HsCD00329505        TCCAAAGGCAAAACAGATCTTCTGGTACTCTTTTTAATGGATCATCAGAAAGATGTTTTT
NM_001077242.1      TCCAAAGGCAAAACAGATCTTCTGGTACTCTTTTTAATGGATCATCAGAAAGATGTTTTT
NM_139160.2         TCCAAAGGCAAAACAGATCTTCTGGTACTCTTTTTAATGGATCATCAGAAAGATGTTTTT
                    ************************************************************

HsCD00329505        AAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTATGGCCATACAGAACGGA
NM_001077242.1      AAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTATGGCCATACAGAACGGA
NM_139160.2         AAGATTCCTGGAACTCTACATAAAATTGTAAGTGTTAAGCTTATGGCCATACAGAACGGA
                    ************************************************************

HsCD00329505        AGAGATCCAAATAGAGATGCAGGATATATTTATTGCCAGAGAATTGATCAACGTGACTAT
NM_001077242.1      AGAGATCCAAATAGAGATGCAGGATATATTTATTGCCAGAGAATTGATCAACGTGACTAT
NM_139160.2         AGAGATCCAAATAGAGATGCAGGATATATTTATTGCCAGAGAATTGATCAACGTGACTAT
                    ************************************************************

HsCD00329505        TCCAACAATATAGAGAAGACAACCAAAGATGAGCTGTTGAATTTACTAAAAACTCTTGAT
NM_001077242.1      TCCAACAATACAGAGAAGACAACCAAAGATGAGCTGTTGAATTTACTAAAAACTCTTGAT
NM_139160.2         TCCAACAATACAGAGAAGACAACCAAAGATGAGCTGTTGAATTTACTAAAAACTCTTGAT
                    ********** *************************************************

HsCD00329505        GAGGATTCAAAACTTTCTGCCAAAGAGAAGAAAAAATTGCTAGGTCAATTCTATAAGTGT
NM_001077242.1      GAGGATTCAAAACTTTCTGCCAAAGAGAAGAAAAAATTGCTAGGTCAATTCTATAAGTGT
NM_139160.2         GAGGATTCAAAACTTTCTGCCAAAGAGAAGAAAAAATTGCTAGGTCAATTCTATAAGTGT
                    ************************************************************

HsCD00329505        CACCCAGACATCTTTATTGAGCATTTTGGAGACTTG
NM_001077242.1      CACCCAGACATCTTTATTGAGCATTTTGGAGACTGA
NM_139160.2         CACCCAGACATCTTTATTGAGCATTTTGGAGACTGA
                    ********************************** .

Pathways

Reactome : Rho GTPase cycle
Reactome : Signal Transduction
Reactome : Signaling by Rho GTPases

Protein

SMART domain : DEP : Domain found in Dishevelled, Egl-10, and Pleckstrin
UniProt : Q96QD5
HPRD : 11280

Clone

Cloning Information : 268395
HIP Master Clone ID : 194879
Original Clone ID : FLH268395.01L

Organism


TAX_ID : 9606
Species Specific ID: 91614

Genome

Chromosome : 11
Map Location : 11p13
Ensembl : ENSG00000121690

Reagents

Labome : DEPDC7-antibody