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ZNF395 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF395
Gene Name:  zinc finger protein 395
Sequence             
Original Clone ID: FLH268397.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329507

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1542nts         Open reading frame : 1 to 1542
Reference Sequence Alignment

ATGGCGAGTGTCCTGTCCCGACGCCTTGGAAAGCGGTCCCTCCTGGGAGCCCGGGTGTTG
GGACCCAGTGCCTCGGAGGGGCCCTCGGCTGCCCCACCCTCGGAGCCACTGCTAGAAGGG
GCCGCTCCCCAGCCTTTCACCACCTCTGATGACACCCCCTGCCAGGAGCAGCCCAAGGAA
GTCCTTAAGGCTCCCAGCACCTCGGGCCTTCAGCAGGTGGCCTTTCAGCCTGGGCAGAAG
GTTTATGTGTGGTACGGGGGTCAAGAGTGCACAGGACTGGTGGAGCAGCACAGCTGGATG
GAGGGTCAGGTGACCGTCTGGCTGCTGGAGCAGAAGCTGCAGGTCTGCTGCAGGGTGGAG
GAGGTGTGGCTGGCAGAGCTGCAGGGCCCCTGTCCCCAGGCACCACCCCTGGAGCCCGGA
GCCCAGGCCCTGGCCTACAGGCCCGTCTCCAGGAACATCGATGTCCCAAAGAGGAAGTCG
GACGCAGTGGAAATGGATGAGATGATGGCGGCCATGGTGCTGACGTCCCTGTCCTGCAGC
CCTGTTGTACAGAGTCCTCCCGGGACCGAGGCCAACTTCTCTGCTTCCCGTGCGGCCTGC
GACCCATGGAAGGAGAGTGGTGACATCTCGGACAGCGGCAGCAGCACTACCAGCGGTCAC
TGGAGTGGGAGCAGTGGTGTCTCCACCCCCTCGCCCCCCCACCCCCAGGCCAGCCCCAAG
TATTTGGGGGATGCTTTTGGTTCTCCCCAAACTGATCATGGCTTTGAGACCGATCCTGAC
CCTTTCCTGCTGGACGAACCAGCTCCACGAAAAAGAAAGAACTCTGTGAAGGTGATGTAC
AAGTGCCTGTGGCCAAACTGTGGCAAAGTTCTGCGCTCCATTGTGGGCATCAAACGACAC
GTCAAAGCCCTCCATCTGGGGGACACAGTGGACTCTGATCAGTTCAAGCGGGAGGAGGAT
TTCTACTACACAGAGGTGCAGCTGAAGGAGGAATCTGCTGCTGCTGCTGCTGCTGCTGCC
GCAGGCACCCCAGTCCCTGGGACTCCCACCTCCGAGCCAGCTCCCACCCCCAGCATGACT
GGCCTGCCTCTGTCTGCTCTTCCACCACCTCTGCACAAAGCCCAGTCCTCCGGCCCAGAA
CATCCTGGCCCGGAGTCCTCCCTGCCCTCAGGGGCTCTCAGCAAGTCAGCTCCTGGGTCC
TTCTGGCACATTCAGGCAGATCATGCATACCAGGCTCTGCCATCCTTCCAGATCCCAGTC
TCACCACACATCTACACCAGTGTCAGCTGGGCTGCTGCCCCCTCCGCCGCCTGCTCTCTC
TCTCCGGTCCGGAGCCGGTCGCTAAGCTTCAGCGAGCCCCAGCAGCCAGCACCTGCGATG
AAATCTCATCTGATCGTCACTTCTCCACCCCGGGCCCAGAGTGGTGCCAGGAAAGCCCGA
GGGGAGGCTAAGAAGTGCCGCAAGGTGTATGGCATCGAGCACCGGGACCAGTGGTGCACG
GCCTGCCGGTGGAAGAAGGCCTGCCAGCGCTTTCTGGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1630
Start on reference sequence 89
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF395
Symbol Nomenclature : ZNF395
SYNONYM : HDBP-2; HDBP2; HDRF-2; PBF; PRF-1; PRF1; Si-1-8-14
Designation : HD gene regulatory region-binding protein 2|HD-regulating factor 2|Huntington's disease gene regulatory region-binding protein 2|huntington disease gene regulatory region-binding protein 2|papillomavirus regulatory factor 1|papillomavirus regulatory factor PRF-1|papillomavirus-binding factor
Full Nomenclature : zinc finger protein 395
GENEID : 55893
HGNC : 18737
MIM : 609494
Vega : OTTHUMG00000102137
Target GenBank: BC001237

 

Reference Sequence Alignment

  

HsCD00329507      ATGGCGAGTGTCCTGTCCCGACGCCTTGGAAAGCGGTCCCTCCTGGGAGCCCGGGTGTTG
NM_018660.2       ATGGCGAGTGTCCTGTCCCGACGCCTTGGAAAGCGGTCCCTCCTGGGAGCCCGGGTGTTG
                  ************************************************************

HsCD00329507      GGACCCAGTGCCTCGGAGGGGCCCTCGGCTGCCCCACCCTCGGAGCCACTGCTAGAAGGG
NM_018660.2       GGACCCAGTGCCTCGGAGGGGCCCTCGGCTGCCCCACCCTCGGAGCCACTGCTAGAAGGG
                  ************************************************************

HsCD00329507      GCCGCTCCCCAGCCTTTCACCACCTCTGATGACACCCCCTGCCAGGAGCAGCCCAAGGAA
NM_018660.2       GCCGCTCCCCAGCCTTTCACCACCTCTGATGACACCCCCTGCCAGGAGCAGCCCAAGGAA
                  ************************************************************

HsCD00329507      GTCCTTAAGGCTCCCAGCACCTCGGGCCTTCAGCAGGTGGCCTTTCAGCCTGGGCAGAAG
NM_018660.2       GTCCTTAAGGCTCCCAGCACCTCGGGCCTTCAGCAGGTGGCCTTTCAGCCTGGGCAGAAG
                  ************************************************************

HsCD00329507      GTTTATGTGTGGTACGGGGGTCAAGAGTGCACAGGACTGGTGGAGCAGCACAGCTGGATG
NM_018660.2       GTTTATGTGTGGTACGGGGGTCAAGAGTGCACAGGACTGGTGGAGCAGCACAGCTGGATG
                  ************************************************************

HsCD00329507      GAGGGTCAGGTGACCGTCTGGCTGCTGGAGCAGAAGCTGCAGGTCTGCTGCAGGGTGGAG
NM_018660.2       GAGGGTCAGGTGACCGTCTGGCTGCTGGAGCAGAAGCTGCAGGTCTGCTGCAGGGTGGAG
                  ************************************************************

HsCD00329507      GAGGTGTGGCTGGCAGAGCTGCAGGGCCCCTGTCCCCAGGCACCACCCCTGGAGCCCGGA
NM_018660.2       GAGGTGTGGCTGGCAGAGCTGCAGGGCCCCTGTCCCCAGGCACCACCCCTGGAGCCCGGA
                  ************************************************************

HsCD00329507      GCCCAGGCCCTGGCCTACAGGCCCGTCTCCAGGAACATCGATGTCCCAAAGAGGAAGTCG
NM_018660.2       GCCCAGGCCCTGGCCTACAGGCCCGTCTCCAGGAACATCGATGTCCCAAAGAGGAAGTCG
                  ************************************************************

HsCD00329507      GACGCAGTGGAAATGGATGAGATGATGGCGGCCATGGTGCTGACGTCCCTGTCCTGCAGC
NM_018660.2       GACGCAGTGGAAATGGATGAGATGATGGCGGCCATGGTGCTGACGTCCCTGTCCTGCAGC
                  ************************************************************

HsCD00329507      CCTGTTGTACAGAGTCCTCCCGGGACCGAGGCCAACTTCTCTGCTTCCCGTGCGGCCTGC
NM_018660.2       CCTGTTGTACAGAGTCCTCCCGGGACCGAGGCCAACTTCTCTGCTTCCCGTGCGGCCTGC
                  ************************************************************

HsCD00329507      GACCCATGGAAGGAGAGTGGTGACATCTCGGACAGCGGCAGCAGCACTACCAGCGGTCAC
NM_018660.2       GACCCATGGAAGGAGAGTGGTGACATCTCGGACAGCGGCAGCAGCACTACCAGCGGTCAC
                  ************************************************************

HsCD00329507      TGGAGTGGGAGCAGTGGTGTCTCCACCCCCTCGCCCCCCCACCCCCAGGCCAGCCCCAAG
NM_018660.2       TGGAGTGGGAGCAGTGGTGTCTCCACCCCCTCGCCCCCCCACCCCCAGGCCAGCCCCAAG
                  ************************************************************

HsCD00329507      TATTTGGGGGATGCTTTTGGTTCTCCCCAAACTGATCATGGCTTTGAGACCGATCCTGAC
NM_018660.2       TATTTGGGGGATGCTTTTGGTTCTCCCCAAACTGATCATGGCTTTGAGACCGATCCTGAC
                  ************************************************************

HsCD00329507      CCTTTCCTGCTGGACGAACCAGCTCCACGAAAAAGAAAGAACTCTGTGAAGGTGATGTAC
NM_018660.2       CCTTTCCTGCTGGACGAACCAGCTCCACGAAAAAGAAAGAACTCTGTGAAGGTGATGTAC
                  ************************************************************

HsCD00329507      AAGTGCCTGTGGCCAAACTGTGGCAAAGTTCTGCGCTCCATTGTGGGCATCAAACGACAC
NM_018660.2       AAGTGCCTGTGGCCAAACTGTGGCAAAGTTCTGCGCTCCATTGTGGGCATCAAACGACAC
                  ************************************************************

HsCD00329507      GTCAAAGCCCTCCATCTGGGGGACACAGTGGACTCTGATCAGTTCAAGCGGGAGGAGGAT
NM_018660.2       GTCAAAGCCCTCCATCTGGGGGACACAGTGGACTCTGATCAGTTCAAGCGGGAGGAGGAT
                  ************************************************************

HsCD00329507      TTCTACTACACAGAGGTGCAGCTGAAGGAGGAATCTGCTGCTGCTGCTGCTGCTGCTGCC
NM_018660.2       TTCTACTACACAGAGGTGCAGCTGAAGGAGGAATCTGCTGCTGCTGCTGCTGCTGCTGCC
                  ************************************************************

HsCD00329507      GCAGGCACCCCAGTCCCTGGGACTCCCACCTCCGAGCCAGCTCCCACCCCCAGCATGACT
NM_018660.2       GCAGGCACCCCAGTCCCTGGGACTCCCACCTCCGAGCCAGCTCCCACCCCCAGCATGACT
                  ************************************************************

HsCD00329507      GGCCTGCCTCTGTCTGCTCTTCCACCACCTCTGCACAAAGCCCAGTCCTCCGGCCCAGAA
NM_018660.2       GGCCTGCCTCTGTCTGCTCTTCCACCACCTCTGCACAAAGCCCAGTCCTCCGGCCCAGAA
                  ************************************************************

HsCD00329507      CATCCTGGCCCGGAGTCCTCCCTGCCCTCAGGGGCTCTCAGCAAGTCAGCTCCTGGGTCC
NM_018660.2       CATCCTGGCCCGGAGTCCTCCCTGCCCTCAGGGGCTCTCAGCAAGTCAGCTCCTGGGTCC
                  ************************************************************

HsCD00329507      TTCTGGCACATTCAGGCAGATCATGCATACCAGGCTCTGCCATCCTTCCAGATCCCAGTC
NM_018660.2       TTCTGGCACATTCAGGCAGATCATGCATACCAGGCTCTGCCATCCTTCCAGATCCCAGTC
                  ************************************************************

HsCD00329507      TCACCACACATCTACACCAGTGTCAGCTGGGCTGCTGCCCCCTCCGCCGCCTGCTCTCTC
NM_018660.2       TCACCACACATCTACACCAGTGTCAGCTGGGCTGCTGCCCCCTCCGCCGCCTGCTCTCTC
                  ************************************************************

HsCD00329507      TCTCCGGTCCGGAGCCGGTCGCTAAGCTTCAGCGAGCCCCAGCAGCCAGCACCTGCGATG
NM_018660.2       TCTCCGGTCCGGAGCCGGTCGCTAAGCTTCAGCGAGCCCCAGCAGCCAGCACCTGCGATG
                  ************************************************************

HsCD00329507      AAATCTCATCTGATCGTCACTTCTCCACCCCGGGCCCAGAGTGGTGCCAGGAAAGCCCGA
NM_018660.2       AAATCTCATCTGATCGTCACTTCTCCACCCCGGGCCCAGAGTGGTGCCAGGAAAGCCCGA
                  ************************************************************

HsCD00329507      GGGGAGGCTAAGAAGTGCCGCAAGGTGTATGGCATCGAGCACCGGGACCAGTGGTGCACG
NM_018660.2       GGGGAGGCTAAGAAGTGCCGCAAGGTGTATGGCATCGAGCACCGGGACCAGTGGTGCACG
                  ************************************************************

HsCD00329507      GCCTGCCGGTGGAAGAAGGCCTGCCAGCGCTTTCTGGACTTG
NM_018660.2       GCCTGCCGGTGGAAGAAGGCCTGCCAGCGCTTTCTGGACTGA
                  **************************************** .

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9H8N7
UniProt : Q7L9C8
HPRD : 11711

Clone

Cloning Information : 268397
HIP Master Clone ID : 194881
Original Clone ID : FLH268397.01L

Organism


TAX_ID : 9606
Species Specific ID: 55893

Genome

Chromosome : 8
Map Location : 8p21.1
Ensembl : ENSG00000186918

Reagents

Labome : HDBP2-antibody