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SPIC (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SPIC
Gene Name:  Spi-C transcription factor (Spi-1/PU.1 related)
Sequence             
Original Clone ID: FLH268400.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329510

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 747nts         Open reading frame : 1 to 747
Reference Sequence Alignment

ATGACGTGTGTTGAACAAGACAAGCTGGGTCAAGCATTTGAAGATGCTTTTGAGGTTCTG
AGGCAACATTCAACTGGAGATCTTCAGTACTCGCCAGATTACAGAAATTACCTGGCTTTA
ATCAACCATCGTCCTCATGTCAAAGGAAATTCCAGCTGCTATGGAGTGTTGCCTACAGAG
GAGCCTGTCTATAATTGGAGAACGGTAATTAACAGTGCTGCGGACTTCTATTTTGAAGGA
AATATTCATCAATCTCTGCAGAACATAACTGAAAACCAGCTGGTACAACCCACTCTTCTC
CAGCAAAAGGGGGGAAAAGGCAGGAAGAAGCTCCGACTGTTTGAATACCTTCACGAATCC
CTGTATAATCCGGAGATGGCATCTTGTATTCAGTGGGTAGATAAAACCAAAGGCATCTTT
CAGTTTGTATCAAAAAACAAAGAAAAACTTGCCGAGCTTTGGGGGAAAAGAAAAGGCAAC
AGGAAGACCATGACTTACCAGAAAATGGCCAGGGCACTCAGAAATTACGGAAGAAGTGGG
GAAATTACCAAAATCCGGAGGAAGCTGACTTACCAGTTCAGTGAGGCCATTCTCCAAAGA
CTCTCTCCATCCTATTTCCTGGGGAAAGAGATCTTCTATTCACAGTGTGTTCAACCTGAT
CAAGAATATCTCAGTTTAAATAACTGGAATGCAAATTATAATTATACATATGCCAATTAC
CATGAGCTAAATCACCATGATTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 773
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SPIC
Symbol Nomenclature : SPIC
SYNONYM : SPI-C
Designation : transcription factor Spi-C
Full Nomenclature : Spi-C transcription factor (Spi-1/PU.1 related)
GENEID : 121599
HGNC : 29549
MIM : 612568
Vega : OTTHUMG00000170273
Target GenBank: BC032317

 

Reference Sequence Alignment

  

HsCD00329510        ATGACGTGTGTTGAACAAGACAAGCTGGGTCAAGCATTTGAAGATGCTTTTGAGGTTCTG
XM_005268647.1      ATGACGTGTGTTGAACAAGACAAGCTGGGTCAAGCATTTGAAGATGCTTTTGAGGTTCTG
XM_005268646.1      ATGACGTGTGTTGAACAAGACAAGCTGGGTCAAGCATTTGAAGATGCTTTTGAGGTTCTG
NM_152323.1         ATGACGTGTGTTGAACAAGACAAGCTGGGTCAAGCATTTGAAGATGCTTTTGAGGTTCTG
                    ************************************************************

HsCD00329510        AGGCAACATTCAACTGGAGATCTTCAGTACTCGCCAGATTACAGAAATTACCTGGCTTTA
XM_005268647.1      AGGCAACATTCAACTGGAGATCTTCAGTACTCGCCAGG----------------------
XM_005268646.1      AGGCAACATTCAACTGGAGATCTTCAGTACTCGCCAGATTACAGAAATTACCTGGCTTTA
NM_152323.1         AGGCAACATTCAACTGGAGATCTTCAGTACTCGCCAGATTACAGAAATTACCTGGCTTTA
                    *************************************.                      

HsCD00329510        ATCAACCATCGTCCTCATGTCAAAGGAAATTCCAGCTGCTATGGAGTGTTGCCTACAGAG
XM_005268647.1      --------------------------AAATTCCAGCTGCTATGGAGTGTTGCCTACAGAG
XM_005268646.1      ATCAACCATCGTCCTCATGTCAAAGGAAATTCCAGCTGCTATGGAGTGTTGCCTACAGAG
NM_152323.1         ATCAACCATCGTCCTCATGTCAAAGGAAATTCCAGCTGCTATGGAGTGTTGCCTACAGAG
                                              **********************************

HsCD00329510        GAGCCTGTCTATAATTGGAGAACGGTAATTAACAGTGCTGCGGACTTCTATTTTGAAGGA
XM_005268647.1      GAGCCTGTCTATAATTGGAGAACGGTAATTAACAGTGCTGCGGACTTCTATTTTGAAGGA
XM_005268646.1      GAGCCTGTCTATAATTGGAGAACGGTAATTAACAGTGCTGCGGACTTCTATTTTGAAGGA
NM_152323.1         GAGCCTGTCTATAATTGGAGAACGGTAATTAACAGTGCTGCGGACTTCTATTTTGAAGGA
                    ************************************************************

HsCD00329510        AATATTCATCAATCTCTGCAGAACATAACTGAAAACCAGCTGGTACAACCCACTCTTCTC
XM_005268647.1      AATATTCATCAATCTCTGCAGAACATAACTGAAAACCAGCTGGTACAACCCACTCTTCTC
XM_005268646.1      AATATTCATCAATCTCTGCAGAACATAACTGAAAACCAGCTGGTACAACCCACTCTTCTC
NM_152323.1         AATATTCATCAATCTCTGCAGAACATAACTGAAAACCAGCTGGTACAACCCACTCTTCTC
                    ************************************************************

HsCD00329510        CAGCAAAAGGGGGGAAAAGGCAGGAAGAAGCTCCGACTGTTTGAATACCTTCACGAATCC
XM_005268647.1      CAGCAAAAGGGGGGAAAAGGCAGGAAGAAGCTCCGACTGTTTGAATACCTTCACGAATCC
XM_005268646.1      CAGCAAAAGGGGGGAAAAGGCAGGAAGAAGCTCCGACTGTTTGAATACCTTCACGAATCC
NM_152323.1         CAGCAAAAGGGGGGAAAAGGCAGGAAGAAGCTCCGACTGTTTGAATACCTTCACGAATCC
                    ************************************************************

HsCD00329510        CTGTATAATCCGGAGATGGCATCTTGTATTCAGTGGGTAGATAAAACCAAAGGCATCTTT
XM_005268647.1      CTGTATAATCCGGAGATGGCATCTTGTATTCAGTGGGTAGATAAAACCAAAGGCATCTTT
XM_005268646.1      CTGTATAATCCGGAGATGGCATCTTGTATTCAGTGGGTAGATAAAACCAAAGGCATCTTT
NM_152323.1         CTGTATAATCCGGAGATGGCATCTTGTATTCAGTGGGTAGATAAAACCAAAGGCATCTTT
                    ************************************************************

HsCD00329510        CAGTTTGTATCAAAAAACAAAGAAAAACTTGCCGAGCTTTGGGGGAAAAGAAAAGGCAAC
XM_005268647.1      CAGTTTGTATCAAAAAACAAAGAAAAACTTGCCGAGCTTTGGGGGAAAAGAAAAGGCAAC
XM_005268646.1      CAGTTTGTATCAAAAAACAAAGAAAAACTTGCCGAGCTTTGGGGGAAAAGAAAAGGCAAC
NM_152323.1         CAGTTTGTATCAAAAAACAAAGAAAAACTTGCCGAGCTTTGGGGGAAAAGAAAAGGCAAC
                    ************************************************************

HsCD00329510        AGGAAGACCATGACTTACCAGAAAATGGCCAGGGCACTCAGAAATTACGGAAGAAGTGGG
XM_005268647.1      AGGAAGACCATGACTTACCAGAAAATGGCCAGGGCACTCAGAAATTACGGAAGAAGTGGG
XM_005268646.1      AGGAAGACCATGACTTACCAGAAAATGGCCAGGGCACTCAGAAATTACGGAAGAAGTGGG
NM_152323.1         AGGAAGACCATGACTTACCAGAAAATGGCCAGGGCACTCAGAAATTACGGAAGAAGTGGG
                    ************************************************************

HsCD00329510        GAAATTACCAAAATCCGGAGGAAGCTGACTTACCAGTTCAGTGAGGCCATTCTCCAAAGA
XM_005268647.1      GAAATTACCAAAATCCGGAGGAAGCTGACTTACCAGTTCAGTGAGGCCATTCTCCAAAGA
XM_005268646.1      GAAATTACCAAAATCCGGAGGAAGCTGACTTACCAGTTCAGTGAGGCCATTCTCCAAAGA
NM_152323.1         GAAATTACCAAAATCCGGAGGAAGCTGACTTACCAGTTCAGTGAGGCCATTCTCCAAAGA
                    ************************************************************

HsCD00329510        CTCTCTCCATCCTATTTCCTGGGGAAAGAGATCTTCTATTCACAGTGTGTTCAACCTGAT
XM_005268647.1      CTCTCTCCATCCTATTTCCTGGGGAAAGAGATCTTCTATTCACAGTGTGTTCAACCTGAT
XM_005268646.1      CTCTCTCCATCCTATTTCCTGGGGAAAGAGATCTTCTATTCACAGTGTGTTCAACCTGAT
NM_152323.1         CTCTCTCCATCCTATTTCCTGGGGAAAGAGATCTTCTATTCACAGTGTGTTCAACCTGAT
                    ************************************************************

HsCD00329510        CAAGAATATCTCAGTTTAAATAACTGGAATGCAAATTATAATTATACATATGCCAATTAC
XM_005268647.1      CAAGAATATCTCAGTTTAAATAACTGGAATGCAAATTATAATTATACATATGCCAATTAC
XM_005268646.1      CAAGAATATCTCAGTTTAAATAACTGGAATGCAAATTATAATTATACATATGCCAATTAC
NM_152323.1         CAAGAATATCTCAGTTTAAATAACTGGAATGCAAATTATAATTATACATATGCCAATTAC
                    ************************************************************

HsCD00329510        CATGAGCTAAATCACCATGATTGCTTG
XM_005268647.1      CATGAGCTAAATCACCATGATTGCTAA
XM_005268646.1      CATGAGCTAAATCACCATGATTGCTAA
NM_152323.1         CATGAGCTAAATCACCATGATTGCTAA
                    *************************:.

Pathways

Panther : Interleukin signaling pathway

Protein

SMART domain : ETS : erythroblast transformation specific domain
UniProt : Q8N5J4
HPRD : 15438

Clone

Cloning Information : 268400
HIP Master Clone ID : 194888
Original Clone ID : FLH268400.01L

Organism


TAX_ID : 9606
Species Specific ID: 121599

Genome

Chromosome : 12
Map Location : 12q23.2
Ensembl : ENSG00000166211

Reagents

Labome : SPI-C-antibody