DNASU Plasmid Repository • 480.965.5697 | Email

ZKSCAN1 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZKSCAN1
Gene Name:  zinc finger with KRAB and SCAN domains 1
Sequence             
Original Clone ID: FLH268405.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329515

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1692nts         Open reading frame : 1 to 1692
Reference Sequence Alignment

ATGATGACTGCTGAATCACGGGAAGCCACGGGTCTGTCCCCACAGGCTGCACAGGAGAAG
GATGGTATCGTAATAGCGAAGGTGGAAGAGGAAGATGAGGAAGACCACATGTGGGGGCAG
GATTCCACCCTACAGGACACGCCTCCTCCAGACCCAGAGATATTCCGCCAACGCTTCAGG
CGCTTCTGTTACCAGAACACTTTTGGGCCCCGAGAGGCTCTCAGTCGGCTGAAGGAACTT
TGTCATCAGTGGCTGCGGCCAGAAATAAACACCAAGGAACAGATCCTGGAGCTTCTGGTG
CTAGAGCAGTTTCTTTCCATCCTGCCCAAGGAGCTCCAGGTCTGGCTGCAGGAATACCGC
CCCGATAGTGGAGAGGAGGCCGTGACCCTTCTAGAAGACTTGGAGCTTGATTTATCAGGA
CAACAGGTCCCAGGTCAAGTTCATGGACCTGAGATGCTCGCAAGGGGGATGGTGCCTCTG
GATCCAGTTCAGGAGTCCTCGAGCTTTGACCTTCATCACGAGGCCACCCAGTCCCACTTC
AAACATTCGTCTCGGAAACCCCGCCTCTTACAGTCACGAGCTCTTCCTGCTGCCCACATT
CCTGCACCCCCTCATGAGGGTAGTCCCAGAGACCAGGCGATGGCATCTGCACTATTCACA
GCGGATTCCCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCATTCTGGAGGAA
TGGGGATGTCAGAATCTGGCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTAT
GGGAGCGCATTTCCCCAGGGTGGTGAAAACAGGAATGAGAACGAGGAGTCAACCTCAAAG
GCTGAAACCTCGGAAGATTCAGCATCACGCGGGGAGACAACAGGAAGATCCCAGAAAGAG
TTTGGAGAGAAACGTGACCAGGAGGGCAAAACAGGAGAAAGACAGCAGAAAAACCCTGAG
GAGAAAACCAGGAAAGAGAAAAGAGATTCAGGGCCAGCTATAGGAAAGGACAAAAAAACC
ATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGATTGGGAAGAAGCTTCAGTCTG
AGCTCCAACTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTCACAGATGTGAT
GAATGTGGTAAATGCTTCACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACACT
GGAGAAAAGCCCTATGAATGTAGTGAGTGTGGGAAAGCCTTCAGTCTTAACTCCAACCTT
GTCCTGCATCAGAGGATCCACACAGGAGAGAAACCTCATGAATGTAACGAGTGTGGCAAG
GCCTTCAGCCACAGTTCCAATCTCATCCTCCATCAGCGCATCCACTCTGGAGAGAAACCT
TATGAATGTAATGAGTGCGGGAAGGCCTTCAGCCAGAGCTCGGACCTCACCAAGCATCAG
AGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAATGTGGAAAAGCTTTCAACCGA
AACTCATACCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCTACAAGTGCACT
AAGTGTGGCAAGGCCTTCACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGCC
AGAGAGAGAGCCTCTGAGTACAGCCCAGCCTCCCTTGATGCATTTGGCGCGTTCCTGAAA
AGTTGTGAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1908
Start on reference sequence 217
Mutations:
None
Discrepancy:
t1688a,V563E
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZKSCAN1
Symbol Nomenclature : ZKSCAN1
SYNONYM : 9130423L19Rik; KOX18; PHZ-37; ZNF139; ZNF36; ZSCAN33
Designation : zinc finger protein 139|zinc finger protein 36 (KOX 18)|zinc finger protein KOX18|zinc finger protein with KRAB and SCAN domains 1
Full Nomenclature : zinc finger with KRAB and SCAN domains 1
GENEID : 7586
HGNC : 13101
MIM : 601260
Vega : OTTHUMG00000156534
Target GenBank: BC022378

 

Reference Sequence Alignment

  

HsCD00329515        ATGATGACTGCTGAATCACGGGAAGCCACGGGTCTGTCCCCACAGGCTGCACAGGAGAAG
NM_001287054.1      ------------------------------------------------------------
NM_003439.2         ATGATGACTGCTGAATCACGGGAAGCCACGGGTCTGTCCCCACAGGCTGCACAGGAGAAG
                                                                                

HsCD00329515        GATGGTATCGTAATAGCGAAGGTGGAAGAGGAAGATGAGGAAGACCACATGTGGGGGCAG
NM_001287054.1      ------------------------------------------------ATGTGGGGGCAG
NM_003439.2         GATGGTATCGTAATAGTGAAGGTGGAAGAGGAAGATGAGGAAGACCACATGTGGGGGCAG
                                                                    ************

HsCD00329515        GATTCCACCCTACAGGACACGCCTCCTCCAGACCCAGAGATATTCCGCCAACGCTTCAGG
NM_001287054.1      GATTCCACCCTACAGGACACGCCTCCTCCAGACCCAGAGATATTCCGCCAACGCTTCAGG
NM_003439.2         GATTCCACCCTACAGGACACGCCTCCTCCAGACCCAGAGATATTCCGCCAACGCTTCAGG
                    ************************************************************

HsCD00329515        CGCTTCTGTTACCAGAACACTTTTGGGCCCCGAGAGGCTCTCAGTCGGCTGAAGGAACTT
NM_001287054.1      CGCTTCTGTTACCAGAACACTTTTGGGCCCCGAGAGGCTCTCAGTCGGCTGAAGGAACTT
NM_003439.2         CGCTTCTGTTACCAGAACACTTTTGGGCCCCGAGAGGCTCTCAGTCGGCTGAAGGAACTT
                    ************************************************************

HsCD00329515        TGTCATCAGTGGCTGCGGCCAGAAATAAACACCAAGGAACAGATCCTGGAGCTTCTGGTG
NM_001287054.1      TGTCATCAGTGGCTGCGGCCAGAAATAAACACCAAGGAACAGATCCTGGAGCTTCTGGTG
NM_003439.2         TGTCATCAGTGGCTGCGGCCAGAAATAAACACCAAGGAACAGATCCTGGAGCTTCTGGTG
                    ************************************************************

HsCD00329515        CTAGAGCAGTTTCTTTCCATCCTGCCCAAGGAGCTCCAGGTCTGGCTGCAGGAATACCGC
NM_001287054.1      CTAGAGCAGTTTCTTTCCATCCTGCCCAAGGAGCTCCAGGTCTGGCTGCAGGAATACCGC
NM_003439.2         CTAGAGCAGTTTCTTTCCATCCTGCCCAAGGAGCTCCAGGTCTGGCTGCAGGAATACCGC
                    ************************************************************

HsCD00329515        CCCGATAGTGGAGAGGAGGCCGTGACCCTTCTAGAAGACTTGGAGCTTGATTTATCAGGA
NM_001287054.1      CCCGATAGTGGAGAGGAGGCCGTGACCCTTCTAGAAGACTTGGAGCTTGATTTATCAGGA
NM_003439.2         CCCGATAGTGGAGAGGAGGCCGTGACCCTTCTAGAAGACTTGGAGCTTGATTTATCAGGA
                    ************************************************************

HsCD00329515        CAACAGGTCCCAGGTCAAGTTCATGGACCTGAGATGCTCGCAAGGGGGATGGTGCCTCTG
NM_001287054.1      CAACAGGTCCCAGGTCAAGTTCATGGACCTGAGATGCTCGCAAGGGGGATGGTGCCTCTG
NM_003439.2         CAACAGGTCCCAGGTCAAGTTCATGGACCTGAGATGCTCGCAAGGGGGATGGTGCCTCTG
                    ************************************************************

HsCD00329515        GATCCAGTTCAGGAGTCCTCGAGCTTTGACCTTCATCACGAGGCCACCCAGTCCCACTTC
NM_001287054.1      GATCCAGTTCAGGAGTCCTCGAGCTTTGACCTTCATCACGAGGCCACCCAGTCCCACTTC
NM_003439.2         GATCCAGTTCAGGAGTCCTCGAGCTTTGACCTTCATCACGAGGCCACCCAGTCCCACTTC
                    ************************************************************

HsCD00329515        AAACATTCGTCTCGGAAACCCCGCCTCTTACAGTCACGAGCTCTTCCTGCTGCCCACATT
NM_001287054.1      AAACATTCGTCTCGGAAACCCCGCCTCTTACAGTCACGAGCTCTTCCTGCTGCCCACATT
NM_003439.2         AAACATTCGTCTCGGAAACCCCGCCTCTTACAGTCACGAGCTCTTCCTGCTGCCCACATT
                    ************************************************************

HsCD00329515        CCTGCACCCCCTCATGAGGGTAGTCCCAGAGACCAGGCGATGGCATCTGCACTATTCACA
NM_001287054.1      CCTGCACCCCCTCATGAGGGTAGTCCCAGAGACCAGGCGATGGCATCTGCACTATTCACA
NM_003439.2         CCTGCACCCCCTCATGAGGGTAGTCCCAGAGACCAGGCGATGGCATCTGCACTATTCACA
                    ************************************************************

HsCD00329515        GCGGATTCCCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCATTCTGGAGGAA
NM_001287054.1      GCGGATTCCCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCATTCTGGAGGAA
NM_003439.2         GCGGATTCCCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCATTCTGGAGGAA
                    ************************************************************

HsCD00329515        TGGGGATGTCAGAATCTGGCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTAT
NM_001287054.1      TGGGGATGTCAGAATCTGGCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTAT
NM_003439.2         TGGGGATGTCAGAATCTGGCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTAT
                    ************************************************************

HsCD00329515        GGGAGCGCATTTCCCCAGGGTGGTGAAAACAGGAATGAGAACGAGGAGTCAACCTCAAAG
NM_001287054.1      GGGAGCGCATTTCCCCAGGGTGGTGAAAACAGGAATGAGAACGAGGAGTCAACCTCAAAG
NM_003439.2         GGGAGCGCATTTCCCCAGGGTGGTGAAAACAGGAATGAGAACGAGGAGTCAACCTCAAAG
                    ************************************************************

HsCD00329515        GCTGAAACCTCGGAAGATTCAGCATCACGCGGGGAGACAACAGGAAGATCCCAGAAAGAG
NM_001287054.1      GCTGAAACCTCGGAAGATTCAGCATCACGCGGGGAGACAACAGGAAGATCCCAGAAAGAG
NM_003439.2         GCTGAAACCTCGGAAGATTCAGCATCACGCGGGGAGACAACAGGAAGATCCCAGAAAGAG
                    ************************************************************

HsCD00329515        TTTGGAGAGAAACGTGACCAGGAGGGCAAAACAGGAGAAAGACAGCAGAAAAACCCTGAG
NM_001287054.1      TTTGGAGAGAAACGTGACCAGGAGGGCAAAACAGGAGAAAGACAGCAGAAAAACCCTGAG
NM_003439.2         TTTGGAGAGAAACGTGACCAGGAGGGCAAAACAGGAGAAAGACAGCAGAAAAACCCTGAG
                    ************************************************************

HsCD00329515        GAGAAAACCAGGAAAGAGAAAAGAGATTCAGGGCCAGCTATAGGAAAGGACAAAAAAACC
NM_001287054.1      GAGAAAACCAGGAAAGAGAAAAGAGATTCAGGGCCAGCTATAGGAAAGGACAAAAAAACC
NM_003439.2         GAGAAAACCAGGAAAGAGAAAAGAGATTCAGGGCCAGCTATAGGAAAGGACAAAAAAACC
                    ************************************************************

HsCD00329515        ATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGATTGGGAAGAAGCTTCAGTCTG
NM_001287054.1      ATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGATTGGGAAGAAGCTTCAGTCTG
NM_003439.2         ATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGATTGGGAAGAAGCTTCAGTCTG
                    ************************************************************

HsCD00329515        AGCTCCAACTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTCACAGATGTGAT
NM_001287054.1      AGCTCCAACTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTCACAGATGTGAT
NM_003439.2         AGCTCCAACTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTCACAGATGTGAT
                    ************************************************************

HsCD00329515        GAATGTGGTAAATGCTTCACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACACT
NM_001287054.1      GAATGTGGTAAATGCTTCACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACACT
NM_003439.2         GAATGTGGTAAATGCTTCACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACACT
                    ************************************************************

HsCD00329515        GGAGAAAAGCCCTATGAATGTAGTGAGTGTGGGAAAGCCTTCAGTCTTAACTCCAACCTT
NM_001287054.1      GGAGAAAAGCCCTATGAATGTAGTGAGTGTGGGAAAGCCTTCAGTCTTAACTCCAACCTT
NM_003439.2         GGAGAAAAGCCCTATGAATGTAGTGAGTGTGGGAAAGCCTTCAGTCTTAACTCCAACCTT
                    ************************************************************

HsCD00329515        GTCCTGCATCAGAGGATCCACACAGGAGAGAAACCTCATGAATGTAACGAGTGTGGCAAG
NM_001287054.1      GTCCTGCATCAGAGGATCCACACAGGAGAGAAACCTCATGAATGTAACGAGTGTGGCAAG
NM_003439.2         GTCCTGCATCAGAGGATCCACACAGGAGAGAAACCTCATGAATGTAACGAGTGTGGCAAG
                    ************************************************************

HsCD00329515        GCCTTCAGCCACAGTTCCAATCTCATCCTCCATCAGCGCATCCACTCTGGAGAGAAACCT
NM_001287054.1      GCCTTCAGCCACAGTTCCAATCTCATCCTCCATCAGCGCATCCACTCTGGAGAGAAACCT
NM_003439.2         GCCTTCAGCCACAGTTCCAATCTCATCCTCCATCAGCGCATCCACTCTGGAGAGAAACCT
                    ************************************************************

HsCD00329515        TATGAATGTAATGAGTGCGGGAAGGCCTTCAGCCAGAGCTCGGACCTCACCAAGCATCAG
NM_001287054.1      TATGAATGTAATGAGTGCGGGAAGGCCTTCAGCCAGAGCTCGGACCTCACCAAGCATCAG
NM_003439.2         TATGAATGTAATGAGTGCGGGAAGGCCTTCAGCCAGAGCTCGGACCTCACCAAGCATCAG
                    ************************************************************

HsCD00329515        AGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAATGTGGAAAAGCTTTCAACCGA
NM_001287054.1      AGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAATGTGGAAAAGCTTTCAACCGA
NM_003439.2         AGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAATGTGGAAAAGCTTTCAACCGA
                    ************************************************************

HsCD00329515        AACTCATACCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCTACAAGTGCACT
NM_001287054.1      AACTCATACCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCTACAAGTGCACT
NM_003439.2         AACTCATACCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCTACAAGTGCACT
                    ************************************************************

HsCD00329515        AAGTGTGGCAAGGCCTTCACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGCC
NM_001287054.1      AAGTGTGGCAAGGCCTTCACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGCC
NM_003439.2         AAGTGTGGCAAGGCCTTCACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGCC
                    ************************************************************

HsCD00329515        AGAGAGAGAGCCTCTGAGTACAGCCCAGCCTCCCTTGATGCATTTGGCGCGTTCCTGAAA
NM_001287054.1      AGAGAGAGAGCCTCTGAGTACAGCCCAGCCTCCCTTGATGCATTTGGCGCGTTCCTGAAA
NM_003439.2         AGAGAGAGAGCCTCTGAGTACAGCCCAGCCTCCCTTGATGCATTTGGCGCGTTCCTGAAA
                    ************************************************************

HsCD00329515        AGTTGTGAGTTG
NM_001287054.1      AGTTGTGTGTAA
NM_003439.2         AGTTGTGTGTAA
                    *******:**:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
SMART domain : SCAN : leucine rich region
UniProt : Q96FA2
UniProt : P17029
HPRD : 03161

Clone

Cloning Information : 268405
HIP Master Clone ID : 194895
Original Clone ID : FLH268405.01L

Organism


TAX_ID : 9606
Species Specific ID: 7586

Genome

Chromosome : 7
Map Location : 7q22
Ensembl : ENSG00000106261

Reagents

Labome : ZKSCAN1-antibody