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RFPL1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RFPL1
Gene Name:  ret finger protein-like 1
Sequence             
Original Clone ID: FLH268406.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329516

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 864nts         Open reading frame : 1 to 864
Reference Sequence Alignment

ATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGTCCCGTCTGCTCAGACTATCTAGAGAAA
CCAATGTCCCTGGAGTGTGGATGCGCTGTCTGCTTCAAGTGCATCAATTCACTGCAGAAG
GAGCCCCATGGGGAGGATCTACTTTGCTGTTGCTGTTCCATGGTCTCTCAGAAGAACAAA
ATCAGGCCCAGTTGGCAGCTAGAGAGGCTGGCTTCCCACATCAAGGAACTGGAGCCCAAG
CTGAAGAAGATTCTGCAGATGAACCCAAGGATGCGGAAGTTCCAAGTGGATACGACCTTG
GATGCCGACACAGCCAACAACTTCCTCATTTCTGACGACCTCAGGAGCGTCCGAAGTGGG
TGCATCACACAGAATCGGCAAGACCTTGCCGAGAGATTTGACGTGTCCATTTGCATCCTG
GGCTCCCCTCGCTTTACCTGTGGCCGCCACTACTGGGAGGTGGACGTGGGAACAAGCACA
GAATGGGACCTGGGAGTCTGCAGAGAATCTGTTCACCGCAAAGGGAGGATCCATCTGACC
ACAGAGCGTGGATTCTGGACTGTGAGTTTGAGGGATGGAAGCCGCCTCTCTGCCAGCACG
GTGCCGCTGACTTTCCTCTTCGTAGACCGCAAGTTACAGCGAGTGGGGATTTTTCTGGAT
ATGGGCATGCAGAACGTTTCCTTTTTTGATGCTGAAGGTGGTTCCCATGTCTATACATTC
AGGAGTGTCTCTGCTGAGGAGCCACTGCACTTGTTTTTTGCTCCTCCAAGTCCACCTAAT
GGTGATAAGAGTGTCTTGAGTATCTGTCCTGTGATAAACCCGGGCACTACTGATGCTCCA
GTCCATCCTGGGGAGGCCAAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 890
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RFPL1
Symbol Nomenclature : RFPL1
SYNONYM : RNF78
Designation : RING finger protein 78
Full Nomenclature : ret finger protein-like 1
GENEID : 5988
HGNC : 9977
MIM : 605968
Vega : OTTHUMG00000150516
Target GenBank: BC104768

 

Reference Sequence Alignment

  

HsCD00329516        ------------------------------------------------------------
NM_021026.2         ATGAAAAGGTTGTCACTTGTCACAACTAACAGGCTTTCACCTCACGGAAATTTTCTTCCC
NM_006604.2         ------------------------------------------------------------
NM_001098535.1      ATGAAAAGGTTGTCACTTGTCACAACTAACAGGCTTTCACCTCAAGGAAATTTTCTTCCC
                                                                                

HsCD00329516        ---------------------------ATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGT
NM_021026.2         TTGTGCACTTTTCCCCTGGCAGTGGACATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGT
NM_006604.2         ---------------------------ATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGT
NM_001098535.1      TTGTGTACTTTTCCCCTGGCAGTGGACATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGT
                                               *********************************

HsCD00329516        CCCGTCTGCTCAGACTATCTAGAGAAACCAATGTCCCTGGAGTGTGGATGCGCTGTCTGC
NM_021026.2         CCCGTCTGCTCAGACTATCTAGAGAAACCAATGTCCCTGGAGTGTGGATGCGCTGTCTGC
NM_006604.2         CCCGTCTGCTCAGACTATCTGGAAAAACCAATGTCCCTGGAGTGTGGATGCACCGTCTGC
NM_001098535.1      CCCGTCTGCTCAGACTATCTGGAAAAACCAATGTCCCTGGAGTGTGGATGCACCGTCTGC
                    ********************.**.***************************.* ******

HsCD00329516        TTCAAGTGCATCAATTCACTGCAGAAGGAGCCCCATGGGGAGGATCTACTTTGCTGTTGC
NM_021026.2         TTCAAGTGCATCAATTCACTGCAGAAGGAGCCCCATGGGGAGGATCTACTTTGCTGTTGC
NM_006604.2         CTCAAGTGCATCAATTCGCTGCAGAAGGAGCCCCATGGGGAGGATCTGCTTTGCTGTTGC
NM_001098535.1      CTCAAGTGCATCAATTCGCTGCAGAAGGAGCCCCATGGGGAGGATCTGCTTTGCTGTTGC
                     ****************.*****************************.************

HsCD00329516        TGTTCCATGGTCTCTCAGAAGAACAAAATCAGGCCCAGTTGGCAGCTAGAGAGGCTGGCT
NM_021026.2         TGTTCCATGGTCTCTCAGAAGAACAAAATCAGGCCCAGTTGGCAGCTAGAGAGGCTGGCT
NM_006604.2         TGTTCCATGGTCTCTCAGAGGAACAAAATCAGGCCCAATCGGCAGCTAGAGAGGCTGGTT
NM_001098535.1      TGTTCCATGGTCTCTCAGAGGAACAAAATCAGGCCCAATCGGCAGCTAGAGAGGCTGGTT
                    *******************.*****************.* ****************** *

HsCD00329516        TCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTGCAGATGAACCCAAGGATG
NM_021026.2         TCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTGCAGATGAACCCAAGGATG
NM_006604.2         TCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTACAGATGAACCCAAGGATG
NM_001098535.1      TCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTACAGATGAACCCAAGGATG
                    *****************************************.******************

HsCD00329516        CGGAAGTTCCAAGTGGATACGACCTTGGATGCCGACACAGCCAACAACT---TCCTCATT
NM_021026.2         CGGAAGTTCCAAGTGGATATGACCTTGGATGCCGACACAGCCAACAACTTCCTCCTCATT
NM_006604.2         CGGAAGTTCCAAGTGGATATGACCTTGGATGCCGACACAGCCAACAACTTCCTCCTCATT
NM_001098535.1      CGGAAGTTCCAAGTGGATATGACCTTGGATGCCGACACAGCCAACAACTTCCTCCTCATT
                    ******************* *****************************   ********

HsCD00329516        TCTGACGACCTCAGGAGCGTCCGAAGTGGGTGCATCACACAGAATCGGCAAGACCTTGCC
NM_021026.2         TCTGACGACCTCAGGAGCGTCCGAAGTGGGTGCATCACACAGAATCGGCAAGACCTTGCC
NM_006604.2         TCTGACGACCTCAGGAGCGTCCGAAGTGGGCTCATCACACAGAATCGGCAAGACCTTGCC
NM_001098535.1      TCTGACGACCTCAGGAGCGTCCGAAGTGGGCTCATCACACAGAATCGGCAAGACCTTGCC
                    ******************************  ****************************

HsCD00329516        GAGAGATTTGACGTGTCCATTTGCATCCTGGGCTCCCCTCGCTTTACCTGTGGCCGCCAC
NM_021026.2         GAGAGATTTGACGTGTCCATTTGCATCCTGGGCTCCCCTCGCTTTACCTGTGGCCGCCAC
NM_006604.2         GAGAGATTTGACGTGTCCGTTTGCATCCTGGGCTCCCCTCGCTTTACCTGTGGCCGCCAC
NM_001098535.1      GAGAGATTTGACGTGTCCGTTTGCATCCTGGGCTCCCCTCGCTTTACCTGTGGCCGCCAC
                    ******************.*****************************************

HsCD00329516        TACTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGTCTGCAGAGAATCT
NM_021026.2         TACTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGTCTGCAGAGAATCT
NM_006604.2         TACTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGTCTGCAGAGAATCT
NM_001098535.1      TACTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGTCTGCAGAGAATCT
                    ************************************************************

HsCD00329516        GTTCACCGCAAAGGGAGGATCCATCTGACCACAGAGCGTGGATTCTGGACTGTGAGTTTG
NM_021026.2         GTTCACCGCAAAGGGAGGATCCATCTGACCACAGAGCGTGGATTCTGGACTGTGAGTTTG
NM_006604.2         GTTCACTGCAAAGGGAAGATCCAGCTGACCACAGAGCTTGGATTCTGGACTGTGAGTTTG
NM_001098535.1      GTTCACTGCAAAGGGAAGATCCAGCTGACCACAGAGCTTGGATTCTGGACTGTGAGTTTG
                    ****** *********.****** ************* **********************

HsCD00329516        AGGGATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCTGACTTTCCTCTTCGTAGACCGC
NM_021026.2         AGGGATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCTGACTTTCCTCTTCGTAGACCGC
NM_006604.2         AGGGATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCTGACTTTCCTCTTAGTAGACCGC
NM_001098535.1      AGGGATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCTGACTTTCCTCTTAGTAGACCGC
                    **************************************************.*********

HsCD00329516        AAGTTACAGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGAT
NM_021026.2         AAGTTACAGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGAT
NM_006604.2         AAGTTACAGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGAT
NM_001098535.1      AAGTTACAGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGAT
                    ************************************************************

HsCD00329516        GCTGAAGGTGGTTCCCATGTCTATACATTCAGGAGTGTCTCTGCTGAGGAGCCACTGCAC
NM_021026.2         GCTGAAGGTGGTTCCCATGTCTATACATTCAGGAGTGTCTCTGCTGAGGAGCCACTGCAC
NM_006604.2         GCTGAAAGTGGTTCCCATGTCTATACATTCAGGAGCGTCTCTGCTGAGGAGCCACTGCGC
NM_001098535.1      GCTGAAAGTGGTTCCCATGTCTATACATTCAGGAGCGTCTCTGCTGAGGAGCCACTGCGC
                    ******.**************************** **********************.*

HsCD00329516        TTGTTTTTTGCTCCTCCAAGTCCACCTAATGGTGATAAGAGTGTCTTGAGTATCTGTCCT
NM_021026.2         TTGTTTTTTGCTCCTCCAAGTCCACCTAATGGTGATAAGAGTGTCTTGAGTATCTGTCCT
NM_006604.2         CCATTTTTGGCTCCTTCAATTCCACCTAATGGTGATCAAGGTGTCTTGAGCATCTGTCCT
NM_001098535.1      CCATTTTTGGCTCCTTCAATTCCACCTAATGGTGATCAAGGTGTCTTGAGCATCTGTCCT
                      .***** ****** *** ****************.*..********** *********

HsCD00329516        GTGATAAACCCGGGCACTACTGATGCTCCAGTCCATCCTGGGGAGGCCAAATTG
NM_021026.2         GTGATAAACCCGGGCACTACTGATGCTCCAGTCCATCCTGGGGAGGCCAAATAA
NM_006604.2         TTGATGAACTCAGGCACTACTGATGCTCCAGTCCGTCCTGGGGAGGCCAAATAA
NM_001098535.1      TTGATGAACTCAGGCACTACTGATGCTCCAGTCCGTCCTGGGGAGGCCAAATAA
                     ****.*** *.**********************.*****************:.

Protein

SMART domain : PRY :
SMART domain : SPRY : Domain in SPla and the RYanodine Receptor.
UniProt : O75677
HPRD : 16182

Clone

Cloning Information : 268406
HIP Master Clone ID : 194898
Original Clone ID : FLH268406.01L

Organism


TAX_ID : 9606
Species Specific ID: 5988

Genome

Chromosome : 22
Map Location : 22q12.2
Ensembl : ENSG00000128250

Reagents

Labome : RFPL1-antibody