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AEBP2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AEBP2
Gene Name:  AE binding protein 2
Sequence             
Original Clone ID: FLH268408.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329518

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 888nts         Open reading frame : 1 to 888
Reference Sequence Alignment

ATGTCGTCGGATGGGGAACCCCTGAGCCGCATGGACTCGGAGGACAGCATAAGCAGTACT
ATAATGGATGTAGACAGCACAATTTCCAGTGGGCGTTCAACTCCAGCAATGATGAATGGA
CAAGGAAGCACTACTTCTTCAAGCAAAAATATTGCCTATAATTGTTGTTGGGACCAGTGC
CAGGCTTGCTTCAACTCTAGCCCAGATCTGGCAGATCACATCCGTTCCATACATGTAGAT
GGTCAGCGAGGAGGGGTATTTGTTTGCTTATGGAAAGGTTGTAAAGTATATAACACTCCA
TCTACCAGTCAAAGTTGGTTACAAAGGCATATGCTGACACACAGTGGAGACAAACCTTTC
AAGTGTGTTGTTGGTGGCTGCAATGCCAGCTTTGCTTCTCAGGGAGGGCTAGCTCGTCAT
GTACCCACACACTTCAGTCAGCAGAACTCCTCAAAAGTTTCTAGCCAGCCAAAGGCCAAA
GAAGAATCTCCTTCTAAAGCTGGAATGAACAAAAGGAGGAAATTAAAGAACAAAAGACGA
CGCTCATTACCACGGCCACATGATTTCTTCGATGCACAAACACTGGATGCGATAAGACAT
CGAGCCATATGCTTTAACCTCTCAGCTCATATAGAAAGTTTAGGGAAGGGACACAGTGTT
GTTTTTCATAGTACTGTAATAGCTAAGAGAAAAGAAGATTCTGGGAAGATCAAACTTTTG
CTTCATTGGATGCCTGAAGACATTCTGCCTGATGTGTGGGTGAATGAAAGTGAACGACAT
CAGTTAAAAACTAAAGTAGTTCATTTATCAAAGCTACCCAAAGATACTGCCTTGCTTTTG
GACCCAAACATATACAGAACAATGCCGCAGAAGAGGTTGAAGAGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1513
Start on reference sequence 626
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AEBP2
Symbol Nomenclature : AEBP2
Designation : AE(adipocyte enhancer)-binding protein 2|AE-binding protein 2|adipocyte enhancer-binding protein 2|zinc finger protein AEBP2
Full Nomenclature : AE binding protein 2
GENEID : 121536
HGNC : 24051
Target GenBank: BC022220

 

Reference Sequence Alignment

  

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         ATGGCCGCCGCTATCACCGACATGGCCGACCTGGAGGAGCTCTCCCGCCTGAGCCCTCTG
NM_001114176.1      ATGGCCGCCGCTATCACCGACATGGCCGACCTGGAGGAGCTCTCCCGCCTGAGCCCTCTG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         CCCCCCGGCAGCCCGGGTTCGGCGGCGCGGGGCCGGGCTGAGCCCCCCGAGGAGGAGGAG
NM_001114176.1      CCCCCCGGCAGCCCGGGTTCGGCGGCGCGGGGCCGGGCTGAGCCCCCCGAGGAGGAGGAG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         GAAGAGGAGGAGGAGGAAGAGGAGGCGGAGGCCGAGGCGGTGGCGGCGCTGCTGCTGAAC
NM_001114176.1      GAAGAGGAGGAGGAGGAAGAGGAGGCGGAGGCCGAGGCGGTGGCGGCGCTGCTGCTGAAC
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         GGCGGCAGCGGTGGGGGCGGCGGAGGCGGCGGCGGAGGAGTGGGGGGCGGCGAGGCAGAG
NM_001114176.1      GGCGGCAGCGGTGGGGGCGGCGGAGGCGGCGGCGGAGGAGTGGGGGGCGGCGAGGCAGAG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         ACGATGTCGGAGCCGAGCCCCGAGAGCGCCAGCCAGGCCGGGGAGGACGAAGACGAGGAG
NM_001114176.1      ACGATGTCGGAGCCGAGCCCCGAGAGCGCCAGCCAGGCCGGGGAGGACGAAGACGAGGAG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         GAGGACGACGAGGAGGAGGAAGATGAGAGCAGCAGCAGCGGCGGGGGTGAGGAGGAGAGT
NM_001114176.1      GAGGACGACGAGGAGGAGGAAGATGAGAGCAGCAGCAGCGGCGGGGGTGAGGAGGAGAGT
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         AGCGCCGAGAGCCTGGTGGGCAGCAGCGGCGGGAGCAGCAGCGACGAGACCCGCTCGTTG
NM_001114176.1      AGCGCCGAGAGCCTGGTGGGCAGCAGCGGCGGGAGCAGCAGCGACGAGACCCGCTCGTTG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         AGCCCCGGCGCCGCCAGCAGCAGCAGCGGGGATGGGGACGGCAAGGAGGGCCTGGAGGAG
NM_001114176.1      AGCCCCGGCGCCGCCAGCAGCAGCAGCGGGGATGGGGACGGCAAGGAGGGCCTGGAGGAG
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         CCCAAGGGACCGCGGGGCAGCCAGGGCGGCGGCGGGGGCGGCAGCAGTAGCAGCAGCGTA
NM_001114176.1      CCCAAGGGACCGCGGGGCAGCCAGGGCGGCGGCGGGGGCGGCAGCAGTAGCAGCAGCGTA
                                                                                

HsCD00329518        ------------------------------------------------------------
NM_001267043.1      ------------------------------------------------------------
NM_153207.4         GTCTCCAGCGGCGGCGACGAGGGCTACGGGACTGGGGGAGGCGGAAGCAGCGCGACCTCC
NM_001114176.1      GTCTCCAGCGGCGGCGACGAGGGCTACGGGACTGGGGGAGGCGGAAGCAGCGCGACCTCC
                                                                                

HsCD00329518        ------------------------ATGTCGTCGGATGGGGAACCCCTGAGCCGCATGGAC
NM_001267043.1      ------------------------------------------------ATGTACACACGT
NM_153207.4         GGGGGCCGGCGGGGCAGCTTGGAGATGTCGTCGGATGGGGAACCCCTGAGCCGCATGGAC
NM_001114176.1      GGGGGCCGGCGGGGCAGCTTGGAGATGTCGTCGGATGGGGAACCCCTGAGCCGCATGGAC
                                                                    *   .** . . 

HsCD00329518        TCGGAGGACAGCATAAGCAGTACTATAATGGATGTAGACAGCACAATTTCCAGTGGGCGT
NM_001267043.1      CGGTACTCAAGCATAAGCAGTACTATAATGGATGTAGACAGCACAATTTCCAGTGGGCGT
NM_153207.4         TCGGAGGACAGCATAAGCAGTACTATAATGGATGTAGACAGCACAATTTCCAGTGGGCGT
NM_001114176.1      TCGGAGGACAGCATAAGCAGTACTATAATGGATGTAGACAGCACAATTTCCAGTGGGCGT
                      * *  ..***************************************************

HsCD00329518        TCAACTCCAGCAATGATGAATGGACAAGGAAGCACTACTTCTTCAAGCAAAAATATTGCC
NM_001267043.1      TCAACTCCAGCAATGATGAATGGACAAGGAAGCACTACTTCTTCAAGCAAAAATATTGCC
NM_153207.4         TCAACTCCAGCAATGATGAATGGACAAGGAAGCACTACTTCTTCAAGCAAAAATATTGCC
NM_001114176.1      TCAACTCCAGCAATGATGAATGGACAAGGAAGCACTACTTCTTCAAGCAAAAATATTGCC
                    ************************************************************

HsCD00329518        TATAATTGTTGTTGGGACCAGTGCCAGGCTTGCTTCAACTCTAGCCCAGATCTGGCAGAT
NM_001267043.1      TATAATTGTTGTTGGGACCAGTGCCAGGCTTGCTTCAACTCTAGCCCAGATCTGGCAGAT
NM_153207.4         TATAATTGTTGTTGGGACCAGTGCCAGGCTTGCTTCAACTCTAGCCCAGATCTGGCAGAT
NM_001114176.1      TATAATTGTTGTTGGGACCAGTGCCAGGCTTGCTTCAACTCTAGCCCAGATCTGGCAGAT
                    ************************************************************

HsCD00329518        CACATCCGTTCCATACATGTAGATGGTCAGCGAGGAGGGGTATTTGTTTGCTTATGGAAA
NM_001267043.1      CACATCCGTTCCATACATGTAGATGGTCAGCGAGGAGGGGTATTTGTTTGCTTATGGAAA
NM_153207.4         CACATCCGTTCCATACATGTAGATGGTCAGCGAGGAGGGGTATTTGTTTGCTTATGGAAA
NM_001114176.1      CACATCCGTTCCATACATGTAGATGGTCAGCGAGGAGGGGTATTTGTTTGCTTATGGAAA
                    ************************************************************

HsCD00329518        GGTTGTAAAGTATATAACACTCCATCTACCAGTCAAAGTTGGTTACAAAGGCATATGCTG
NM_001267043.1      GGTTGTAAAGTATATAACACTCCATCTACCAGTCAAAGTTGGTTACAAAGGCATATGCTG
NM_153207.4         GGTTGTAAAGTATATAACACTCCATCTACCAGTCAAAGTTGGTTACAAAGGCATATGCTG
NM_001114176.1      GGTTGTAAAGTATATAACACTCCATCTACCAGTCAAAGTTGGTTACAAAGGCATATGCTG
                    ************************************************************

HsCD00329518        ACACACAGTGGAGACAAACCTTTCAAGTGTGTTGTTGGTGGCTGCAATGCCAGCTTTGCT
NM_001267043.1      ACACACAGTGGAGACAAACCTTTCAAGTGTGTTGTTGGTGGCTGCAATGCCAGCTTTGCT
NM_153207.4         ACACACAGTGGAGACAAACCTTTCAAGTGTGTTGTTGGTGGCTGCAATGCCAGCTTTGCT
NM_001114176.1      ACACACAGTGGAGACAAACCTTTCAAGTGTGTTGTTGGTGGCTGCAATGCCAGCTTTGCT
                    ************************************************************

HsCD00329518        TCTCAGGGAGGGCTAGCTCGTCATGTACCCACACACTTCAGTCAGCAGAACTCCTCAAAA
NM_001267043.1      TCTCAGGGAGGGCTAGCTCGTCATGTACCCACACACTTCAGTCAGCAGAACTCCTCAAAA
NM_153207.4         TCTCAGGGAGGGCTAGCTCGTCATGTACCCACACACTTCAGTCAGCAGAACTCCTCAAAA
NM_001114176.1      TCTCAGGGAGGGCTAGCTCGTCATGTACCCACACACTTCAGTCAGCAGAACTCCTCAAAA
                    ************************************************************

HsCD00329518        GTTTCTAGCCAGCCAAAGGCCAAAGAAGAATCTCCTTCTAAAGCTGGAATGAACAAAAGG
NM_001267043.1      GTTTCTAGCCAGCCAAAGGCCAAAGAAGAATCTCCTTCTAAAGCTGGAATGAACAAAAGG
NM_153207.4         GTTTCTAGCCAGCCAAAGGCCAAAGAAGAATCTCCTTCTAAAGCTGGAATGAACAAAAGG
NM_001114176.1      GTTTCTAGCCAGCCAAAGGCCAAAGAAGAATCTCCTTCTAAAGCTGGAATGAACAAAAGG
                    ************************************************************

HsCD00329518        AGGAAATTAAAGAACAAAAGACGACGCTCATTACCACGGCCACATGATTTCTTCGATGCA
NM_001267043.1      AGGAAATTAAAGAACAAAAGACGACGCTCATTACCACGGCCACATGATTTCTTCGATGCA
NM_153207.4         AGGAAATTAAAGAACAAAAGACGACGCTCATTACCACGGCCACATGATTTCTTCGATGCA
NM_001114176.1      AGGAAATTAAAGAACAAAAGACGACGCTCATTACCACGGCCACATGATTTCTTCGATGCA
                    ************************************************************

HsCD00329518        CAAACACTGGATGCGATAAGACATCGAGCCATATGCTTTAACCTCTCAGCTCATATAGAA
NM_001267043.1      CAAACACTGGATGCGATAAGACATCGAGCCATATGCTTTAACCTCTCAGCTCATATAGAA
NM_153207.4         CAAACACTGGATGCGATAAGACATCGAGCCATATGCTTTAACCTCTCAGCTCATATAGAA
NM_001114176.1      CAAACACTGGATGCGATAAGACATCGAGCCATATGCTTTAACCTCTCAGCTCATATAGAA
                    ************************************************************

HsCD00329518        AGTTTAGGGAAGGGACACAGTGTTGTTTTTCATAGTACTGTAATAGCTAAGAGAAAAGAA
NM_001267043.1      AGTTTAGGGAAGGGACACAGTGTTGTTTTTCATAGTACTGTAATAGCTAAGAGAAAAGAA
NM_153207.4         AGTTTAGGGAAGGGACACAGTGTTGTTTTTCATAGTACTGTAATAGCTAAGAGAAAAGAA
NM_001114176.1      AGTTTAGGGAAGGGACACAGTGTTGTTTTTCATAGTACTGTAATAGCTAAGAGAAAAGAA
                    ************************************************************

HsCD00329518        GATTCTGGGAAGATCAAACTTTTGCTTCATTGGATGCCTGAAGACATTCTGCCTGATGTG
NM_001267043.1      GATTCTGGGAAGATCAAACTTTTGCTTCATTGGATGCCTGAAGACATTCTGCCTGATGTG
NM_153207.4         GATTCTGGGAAGATCAAACTTTTGCTTCATTGGATGCCTGAAGACATTCTGCCTGATGTG
NM_001114176.1      GATTCTGGGAAGATCAAACTTTTGCTTCATTGGATGCCTGAAGACATTCTGCCTGATGTG
                    ************************************************************

HsCD00329518        TGGGTGAATGAAAGTGAACGACATCAGTTAAAAACTAAAGTAGTTCATTTATCAAAGCTA
NM_001267043.1      TGGGTGAATGAAAGTGAACGACATCAGTTAAAAACTAAAGTAGTTCATTTATCAAAGCTA
NM_153207.4         TGGGTGAATGAAAGTGAACGACATCAGTTAAAAACTAAAGTAGTTCATTTATCAAAGCTA
NM_001114176.1      TGGGTGAATGAAAGTGAACGACATCAGTTAAAAACTAAAGTAGTTCATTTATCAAAGCTA
                    ************************************************************

HsCD00329518        CCCAAAGATACTGCCTTGCTTTTGGACCCAAACATATACAGAACAATGCCGCAGAAGAGG
NM_001267043.1      CCCAAAGATACTGCCTTGCTTTTGGACCCAAACATATACAGAACAATGCCGCAGAAGAGG
NM_153207.4         CCCAAAGATACTGCCTTGCTTTTGGACCCAAACATATACAGAACAATGCCGCAGAAGAGG
NM_001114176.1      CCCAAAGATACTGCCTTGCTTTTGGACCCAAACATATACAGAACAATGCCGCAGAAGAGG
                    ************************************************************

HsCD00329518        TTGAAGAGGTTG------------------------------------------
NM_001267043.1      TTGAAGAGAACTCTGATAAGAAAAGTGTTCAATTTGTATTTAAGCAAACAGTGA
NM_153207.4         TTGAAGAGGTAA------------------------------------------
NM_001114176.1      TTGAAGAGAACTCTGATAAGAAAAGTGTTCAATTTGTATTTAAGCAAACAGTGA
                    ********.:                                            

Pathways

Reactome : Epigenetic regulation of gene expression
Reactome : Gene Expression
Reactome : PRC2 methylates histones and DNA

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q6ZN18
HPRD : 16477

Clone

Cloning Information : 268408
HIP Master Clone ID : 194902
Original Clone ID : FLH268408.01L

Organism


TAX_ID : 9606
Species Specific ID: 121536

Genome

Chromosome : 12
Map Location : 12p12.3

Reagents

Labome : AEBP2-antibody