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CBFA2T3 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CBFA2T3
Gene Name:  core-binding factor, runt domain, alpha subunit 2; translocated to, 3
Sequence             
Original Clone ID: FLH268410.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329520

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1848nts         Open reading frame : 1 to 1848
Reference Sequence Alignment

ATGCCGGCTTCAAGACTGAGGGACAGGGCAGCCAGTTCAGCCTCGGGATCCACCTGTGGC
TCCATGTCCCAGACGCACCCTGTGCTGGAGAGCGGCCTCCTGGCATCTGCCGGCTGCTCC
GCACCCCGGGGTCCCAGGAAGGGCGGCCCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCA
GCGATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATG
CCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACAC
ACACTGATGAACGGCAGCAGCCACTCACCAACAGCCATCAATGGTGCACCGTGCACACCC
AACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTGTCCACACAGCACCTG
CCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTCCTCACCACACTGCAG
CAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGCACACTGGTGCTGGGC
CTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTTCAGGAGGCCACCAAC
TTCCCTCTGCGGCCGTTTGTCATTCCCTTCCTGAAGGCAAACCTGCCCTTGCTGCAGCGG
GAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAGTACTTGGCCCAGCAT
GAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCCTCAGAGCTGCTACTG
GAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAAGAGAACGGGTCAGAC
CGCGACCCGCTGCACCCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCG
CCGCCGCACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGC
CACCCAGACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGG
CAGGAAGAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCAC
CTCAACAACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACG
GTGCTGCGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTAC
AGCGACGCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGC
TCCGCCGGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTC
ACCGGCTACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAG
CGGCAGGCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAG
CTCATCACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCC
TCCGAGGACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAAC
TGCGGGCGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCC
TTCTGCCAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGC
CCCACAGCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGC
CCCTCCCTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGC
CCCGGCTCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2121
Start on reference sequence 274
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CBFA2T3
Symbol Nomenclature : CBFA2T3
SYNONYM : ETO2; MTG16; MTGR2; ZMYND4
Designation : MTG8-related gene 2|MTG8-related protein 2|myeloid translocation gene 8 and 16b|myeloid translocation gene on chromosome 16 protein|protein CBFA2T3|zinc finger MYND domain-containing protein 4
Full Nomenclature : core-binding factor, runt domain, alpha subunit 2; translocated to, 3
GENEID : 863
HGNC : 1537
MIM : 603870
Vega : OTTHUMG00000137864
Target GenBank: BC062624

 

Reference Sequence Alignment

  

HsCD00329520      ATGCCGGCTTCAAGACTGAGGGACAGGGCAGCCAGTTCAGCCTCGGGATCCACCTGTGGC
NM_175931.2       ------------------------------------------------------------
NM_005187.5       ATGCCGGCTTCAAGACTGAGGGACAGGGCAGCCAGTTCAGCCTCGGGATCCACCTGTGGC
                                                                              

HsCD00329520      TCCATGTCCCAGACGCACCCTGTGCTGGAGAGCGGCCTCCTGGCATCTGCCGGCTGCTCC
NM_175931.2       ------------------------------------------------------------
NM_005187.5       TCCATGTCCCAGACGCACCCTGTGCTGGAGAGCGGCCTCCTGGCATCTGCCGGCTGCTCC
                                                                              

HsCD00329520      GCACCCCGGGGTCCCAGGAAGGGCGGCCCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCA
NM_175931.2       ------------------------------------------------------------
NM_005187.5       GCACCCCGGGGTCCCAGGAAGGGCGGCCCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCA
                                                                              

HsCD00329520      GCGATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATG
NM_175931.2       ---ATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATG
NM_005187.5       GCGATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATG
                     *********************************************************

HsCD00329520      CCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACAC
NM_175931.2       CCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACAC
NM_005187.5       CCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACAC
                  ************************************************************

HsCD00329520      ACACTGATG---------------------------------------------------
NM_175931.2       ACACTGATG---------------------------------------------------
NM_005187.5       ACACATCGAGAGGACGGGCCTGCGACGCTGCCCCACGGCCGTTTTCATGGCTGCTTAAAA
                  ****: . .                                                   

HsCD00329520      ------------------------AACGGCAGCAGCCACTCACCAACAGCCATCAATGGT
NM_175931.2       ------------------------AACGGCAGCAGCCACTCACCAACAGCCATCAATGGT
NM_005187.5       TGGTCTATGGTCTGTCTCTTGATGAACGGCAGCAGCCACTCACCAACAGCCATCAATGGT
                                          ************************************

HsCD00329520      GCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTG
NM_175931.2       GCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTG
NM_005187.5       GCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTG
                  ************************************************************

HsCD00329520      TCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTC
NM_175931.2       TCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTC
NM_005187.5       TCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTC
                  ************************************************************

HsCD00329520      CTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGC
NM_175931.2       CTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGC
NM_005187.5       CTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGC
                  ************************************************************

HsCD00329520      ACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTT
NM_175931.2       ACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTT
NM_005187.5       ACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTT
                  ************************************************************

HsCD00329520      CAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCCTTCCTGAAGGCAAACCTG
NM_175931.2       CAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCCTTCCTGAAGGCAAACCTG
NM_005187.5       CAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCCTTCCTGAAGGCAAACCTG
                  ************************************************************

HsCD00329520      CCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAG
NM_175931.2       CCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAG
NM_005187.5       CCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAG
                  ************************************************************

HsCD00329520      TACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCC
NM_175931.2       TACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCC
NM_005187.5       TACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCC
                  ************************************************************

HsCD00329520      TCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAA
NM_175931.2       TCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAA
NM_005187.5       TCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAA
                  ************************************************************

HsCD00329520      GAGAACGGGTCAGACCGCGACCCGCTGCACCCC---------------------------
NM_175931.2       GAGAACGGGTCAGACCGCGACCCGCTGCACCCCGAGCACCTCAGCAAACGGCCATGCACC
NM_005187.5       GAGAACGGGTCAGACCGCGACCCGCTGCACCCCGAGCACCTCAGCAAACGGCCATGCACC
                  *********************************                           

HsCD00329520      ------------CAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCG
NM_175931.2       CTGAACCCTGCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCG
NM_005187.5       CTGAACCCTGCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCG
                              ************************************************

HsCD00329520      CACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCA
NM_175931.2       CACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCA
NM_005187.5       CACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCA
                  ************************************************************

HsCD00329520      GACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAA
NM_175931.2       GACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAA
NM_005187.5       GACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAA
                  ************************************************************

HsCD00329520      GAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAAC
NM_175931.2       GAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAAC
NM_005187.5       GAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAAC
                  ************************************************************

HsCD00329520      AACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTG
NM_175931.2       AACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTG
NM_005187.5       AACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTG
                  ************************************************************

HsCD00329520      CGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGAC
NM_175931.2       CGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGAC
NM_005187.5       CGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGAC
                  ************************************************************

HsCD00329520      GCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCC
NM_175931.2       GCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCC
NM_005187.5       GCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCC
                  ************************************************************

HsCD00329520      GGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGC
NM_175931.2       GGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGC
NM_005187.5       GGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGC
                  ************************************************************

HsCD00329520      TACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAG
NM_175931.2       TACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAG
NM_005187.5       TACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAG
                  ************************************************************

HsCD00329520      GCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATC
NM_175931.2       GCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATC
NM_005187.5       GCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATC
                  ************************************************************

HsCD00329520      ACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAG
NM_175931.2       ACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAG
NM_005187.5       ACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAG
                  ************************************************************

HsCD00329520      GACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGG
NM_175931.2       GACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGG
NM_005187.5       GACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGG
                  ************************************************************

HsCD00329520      CGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGC
NM_175931.2       CGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGC
NM_005187.5       CGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGC
                  ************************************************************

HsCD00329520      CAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACA
NM_175931.2       CAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACA
NM_005187.5       CAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACA
                  ************************************************************

HsCD00329520      GCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCC
NM_175931.2       GCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCC
NM_005187.5       GCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCC
                  ************************************************************

HsCD00329520      CTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGC
NM_175931.2       CTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGC
NM_005187.5       CTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGC
                  ************************************************************

HsCD00329520      TCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTTG
NM_175931.2       TCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTGA
NM_005187.5       TCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTGA
                  **************************************** .

Pathways

NCI : ErbB4 signaling events

Protein

SMART domain : TAFH : TAF homology
UniProt : O75081
HPRD : 04847

Clone

Cloning Information : 268410
HIP Master Clone ID : 194905
Original Clone ID : FLH268410.01L

Organism


TAX_ID : 9606
Species Specific ID: 863

Genome

Chromosome : 16
Map Location : 16q24
Ensembl : ENSG00000129993

Reagents

Labome : CBFA2T3-antibody