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DPF3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DPF3
Gene Name:  D4, zinc and double PHD fingers, family 3
Sequence             
Original Clone ID: FLH268418.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329528

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1074nts         Open reading frame : 1 to 1074
Reference Sequence Alignment

ATGGCGACTGTCATTCACAACCCCCTGAAAGCGCTCGGGGACCAGTTCTACAAGGAAGCC
ATTGAGCACTGCCGGAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCC
TTCCTGGACTCACAGACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGG
CACCGAGGCCCAGGCCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGC
AAGAAGAGACGACTGCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCT
GAAGTGGAGCTTCCCCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCC
TTGCTCCGTGGCGAGGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAG
GAAATACAGAGGGTTTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGAT
TTGGAAGAGGATATTCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCACGGCTCTGCA
GGGGGCAGGAGGAGGCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGT
GACATCTGTGGCAAGCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACT
CACCTGGCCAGCGAGGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAAC
CACAGAAATGAGAACCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAAC
TACTGTGACTTCTGCTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAG
CTGGTGTCCTGCGCAGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAG
AAGGAGGCAGCGGCCGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGT
GACACGTCAACTTTCCGCGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGA
GGACGCCGCAGTGGCCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1087
Start on reference sequence 14
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DPF3
Symbol Nomenclature : DPF3
SYNONYM : BAF45C; CERD4
Designation : BRG1-associated factor 45C|zinc finger protein DPF3|zinc finger protein cer-d4
Full Nomenclature : D4, zinc and double PHD fingers, family 3
GENEID : 8110
HGNC : 17427
MIM : 601672
Vega : OTTHUMG00000171364
Target GenBank: BC060801

 

Reference Sequence Alignment

  

HsCD00329528        ------------------------------------------------------------
NM_001280543.1      ------------------------------------------------------------
NM_012074.4         ------------------------------------------------------------
NM_001280544.1      ATGTTTTATGGCAGAATAAATGGGCGTAACTTCGCCGCATCCTCGCTGCCGGTTGCTTTC
                                                                                

HsCD00329528        ------------------------------------------------------------
NM_001280543.1      ------------------------------------------------------------
NM_012074.4         ------------------------------------------------------------
NM_001280544.1      GCTGCAACACCGCTGATGCTGTTTCTACCGAACCCACAACTGATTTTCAGTTTCCCCATT
                                                                                

HsCD00329528        ---------------------------------------------ATGGCGACTGTCATT
NM_001280543.1      ----------------ATGGGGTTCACAGACCTGGAAGAGCCCATCTCTGGATGCCCTGG
NM_012074.4         ---------------------------------------------ATGGCGACTGTCATT
NM_001280544.1      TCCAGCCGAAATCACATAACCGGGCTGATGCCACCTGGTAAACTCAAGTTAGAGAACCTA
                                                                 .:   ..    *   

HsCD00329528        CACAACCCCCTGAAAGCG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_001280543.1      AGGCCCATGGGCTCTAGGGCTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_012074.4         CACAACCCCCTGAAAGCG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_001280544.1      TTTCACATGTGCACCAGG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
                       ..*.     :. . * *****************************************

HsCD00329528        GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_001280543.1      GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_012074.4         GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_001280544.1      GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
                    ************************************************************

HsCD00329528        GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_001280543.1      GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_012074.4         GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_001280544.1      GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
                    ************************************************************

HsCD00329528        CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGACT
NM_001280543.1      CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
NM_012074.4         CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
NM_001280544.1      CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
                    ********************************************************** *

HsCD00329528        GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_001280543.1      GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_012074.4         GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_001280544.1      GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
                    ************************************************************

HsCD00329528        CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_001280543.1      CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_012074.4         CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_001280544.1      CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
                    ************************************************************

HsCD00329528        GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_001280543.1      GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_012074.4         GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_001280544.1      GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
                    ************************************************************

HsCD00329528        TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_001280543.1      TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_012074.4         TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_001280544.1      TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
                    ************************************************************

HsCD00329528        TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCACGGCTCTGCAGGGGGCAGGAGGAG
NM_001280543.1      TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
NM_012074.4         TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
NM_001280544.1      TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
                    ***********************************.************************

HsCD00329528        GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_001280543.1      GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_012074.4         GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_001280544.1      GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
                    ************************************************************

HsCD00329528        GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_001280543.1      GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_012074.4         GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_001280544.1      GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
                    ************************************************************

HsCD00329528        GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_001280543.1      GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_012074.4         GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_001280544.1      GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
                    ************************************************************

HsCD00329528        CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_001280543.1      CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_012074.4         CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_001280544.1      CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
                    ************************************************************

HsCD00329528        CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_001280543.1      CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_012074.4         CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_001280544.1      CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
                    ************************************************************

HsCD00329528        AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_001280543.1      AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_012074.4         AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_001280544.1      AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
                    ************************************************************

HsCD00329528        CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_001280543.1      CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_012074.4         CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_001280544.1      CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
                    ************************************************************

HsCD00329528        CCGCGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_001280543.1      CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_012074.4         CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_001280544.1      CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
                    **.*********************************************************

HsCD00329528        CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTTG
NM_001280543.1      CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
NM_012074.4         CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
NM_001280544.1      CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
                    **************************************:.

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q92784
HPRD : 03395

Clone

Cloning Information : 268418
HIP Master Clone ID : 194925
Original Clone ID : FLH268418.01L

Organism


TAX_ID : 9606
Species Specific ID: 8110

Genome

Chromosome : 14
Map Location : 14q24.2
Ensembl : ENSG00000205683

Reagents

Labome : DPF3-antibody