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HSFY1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  HSFY1
Gene Name:  heat shock transcription factor, Y-linked 1
Sequence             
Original Clone ID: FLH268423.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329533

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1206nts         Open reading frame : 1 to 1206
Reference Sequence Alignment

ATGGCACATGTTTCTTCAGAAACTCAAGATGTTTCCCCCAAAGATGAATTAACTGCTTCA
GAAGCCTCCACTAGGTCTCCATTGTGTGAACACACCTTCCCTGGGGACTCAGACTTACGG
TCAATGATTGAAGAACATGCTTTTCAGGTTTTGTCACAAGGATCCTTGTTAGAAAGTCCA
AGTTACACAGTTTGTGTCTCTGAGCCAGATAAAGATGATGATTTTCTTTCTCTGAACTTT
CCCAGGAAACTTTGGAAAATAGTGGAAAGTGACCAATTCAAGTCTATTTCATGGGATGAG
AATGGAACTTGCATAGTGATTAATGAAGAACTCTTCAAGAAAGAAATTTTGGAAACAAAG
GCTCCTTACAGAATATTTCAAACTGATGCTATCAAAAGTTTTGTTCGACAGCTCAACCTT
TATGGATTTAGTAAAATTCAACAGAATTTTCAAAGATCTGCCTTTCTAGCCACCTTTCTG
TCAGAAGAGAAAGAATCGTCTGTCTTAAGCAAGTTAAAGTTCTATTATAATCCAAATTTC
AAGCGTGGCTATCCCCAACTTTTAGTAAGAGTGAAGAGAAGAATTGGTGTTAAAAATGCT
TCACCTATATCTACTTTATTCAACGAAGATTTCAACAAGAAGCATTTTAGAGCAGGGGCT
AACATGGAGAATCATAATTCTGCCTTAGCTGCTGAAGCTAGTGAAGAAAGTTTATTTTCA
GCCTCTAAAAATTTAAATATGCCTCTAACAAGGGAATCTTCTGTCAGACAGATAATTGCA
AATTCATCTGTCCCCATTAGAAGTGGTTTCCCTCCTCCTTCACCTTCAACCTCAGTTGGA
CCATCAGAACAAATTGCAACAGATCAACATGCTATTTTAAATCAGTTGACCACTATTCAT
ATGCACTCTCATAGTACCTACATGCAAGCAAGGGGCCACATTGTGAATTTTATTACAACC
ACAACTTCTCAATACCACATCATATCTCCCTTACAAAATGGTTATTTTGGGCTGACAGTG
GAACCATCTGCTGTTCCCACACGATATCCTCTGGTATCAGTCAATGAGGCTCCATATCGT
AACATGCTACCAGCAGGCAACCCGTGGTTGCAAATGCCTACGATCGCTGATAGATCAGCT
GCCCCTCATTCCAGGCTAGCTCTTCAACCATCACCACTGGACAAATATCACCCTAATTAC
AACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1306
Start on reference sequence 101
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HSFY1
Symbol Nomenclature : HSFY1
SYNONYM : HSF2L; HSFY
Designation : heat shock transcription factor 2-like protein|heat shock transcription factor, Y-linked
Full Nomenclature : heat shock transcription factor, Y-linked 1
GENEID : 86614
HGNC : 18568
MIM : 400029
Vega : OTTHUMG00000041980
Target GenBank: BC036567

 

Reference Sequence Alignment

  

HsCD00329533      ATGGCACATGTTTCTTCAGAAACTCAAGATGTTTCCCCCAAAGATGAATTAACTGCTTCA
NM_153716.1       ATGGCACATGTTTCTTCAGAAACTCAAGATGTTTCCCCCAAAGATGAATTAACTGCTTCA
NM_033108.2       ATGGCACATGTTTCTTCAGAAACTCAAGATGTTTCCCCCAAAGATGAATTAACTGCTTCA
                  ************************************************************

HsCD00329533      GAAGCCTCCACTAGGTCTCCATTGTGTGAACACACCTTCCCTGGGGACTCAGACTTACGG
NM_153716.1       GAAGCCTCCACTAGGTCTCCATTGTGTGAACACACCTTCCCTGGGGACTCAGACTTACGG
NM_033108.2       GAAGCCTCCACTAGGTCTCCATTGTGTGAACACACCTTCCCTGGGGACTCAGACTTACGG
                  ************************************************************

HsCD00329533      TCAATGATTGAAGAACATGCTTTTCAGGTTTTGTCACAAGGATCCTTGTTAGAAAGTCCA
NM_153716.1       TCAATGATTGAAGAACATGCTTTTCAGGTTTTGTCACAAGGATCCTTGTTAGAAAGTCCA
NM_033108.2       TCAATGATTGAAGAACATGCTTTTCAGGTTTTGTCACAAGGATCCTTGTTAGAAAGTCCA
                  ************************************************************

HsCD00329533      AGTTACACAGTTTGTGTCTCTGAGCCAGATAAAGATGATGATTTTCTTTCTCTGAACTTT
NM_153716.1       AGTTACACAGTTTGTGTCTCTGAGCCAGATAAAGATGATGATTTTCTTTCTCTGAACTTT
NM_033108.2       AGTTACACAGTTTGTGTCTCTGAGCCAGATAAAGATGATGATTTTCTTTCTCTGAACTTT
                  ************************************************************

HsCD00329533      CCCAGGAAACTTTGGAAAATAGTGGAAAGTGACCAATTCAAGTCTATTTCATGGGATGAG
NM_153716.1       CCCAGGAAACTTTGGAAAATAGTGGAAAGTGACCAATTCAAGTCTATTTCATGGGATGAG
NM_033108.2       CCCAGGAAACTTTGGAAAATAGTGGAAAGTGACCAATTCAAGTCTATTTCATGGGATGAG
                  ************************************************************

HsCD00329533      AATGGAACTTGCATAGTGATTAATGAAGAACTCTTCAAGAAAGAAATTTTGGAAACAAAG
NM_153716.1       AATGGAACTTGCATAGTGATTAATGAAGAACTCTTCAAGAAAGAAATTTTGGAAACAAAG
NM_033108.2       AATGGAACTTGCATAGTGATTAATGAAGAACTCTTCAAGAAAGAAATTTTGGAAACAAAG
                  ************************************************************

HsCD00329533      GCTCCTTACAGAATATTTCAAACTGATGCTATCAAAAGTTTTGTTCGACAGCTCAACCTT
NM_153716.1       GCTCCTTACAGAATATTTCAAACTGATGCTATCAAAAGTTTTGTTCGACAGCTCAACCTT
NM_033108.2       GCTCCTTACAGAATATTTCAAACTGATGCTATCAAAAGTTTTGTTCGACAGCTCAACCTT
                  ************************************************************

HsCD00329533      TATGGATTTAGTAAAATTCAACAGAATTTTCAAAGATCTGCCTTTCTAGCCACCTTTCTG
NM_153716.1       TATGGATTTAGTAAAATTCAACAGAATTTTCAAAGATCTGCCTTTCTAGCCACCTTTCTG
NM_033108.2       TATGGATTTAGTAAAATTCAACAGAATTTTCAAAGATCTGCCTTTCTAGCCACCTTTCTG
                  ************************************************************

HsCD00329533      TCAGAAGAGAAAGAATCGTCTGTCTTAAGCAAGTTAAAGTTCTATTATAATCCAAATTTC
NM_153716.1       TCAGAAGAGAAAGAATCGTCTGTCTTAAGCAAGTTAAAGTTCTATTATAATCCAAATTTC
NM_033108.2       TCAGAAGAGAAAGAATCGTCTGTCTTAAGCAAGTTAAAGTTCTATTATAATCCAAATTTC
                  ************************************************************

HsCD00329533      AAGCGTGGCTATCCCCAACTTTTAGTAAGAGTGAAGAGAAGAATTGGTGTTAAAAATGCT
NM_153716.1       AAGCGTGGCTATCCCCAACTTTTAGTAAGAGTGAAGAGAAGAATTGGTGTTAAAAATGCT
NM_033108.2       AAGCGTGGCTATCCCCAACTTTTAGTAAGAGTGAAGAGAAGAATTGGTGTTAAAAATGCT
                  ************************************************************

HsCD00329533      TCACCTATATCTACTTTATTCAACGAAGATTTCAACAAGAAGCATTTTAGAGCAGGGGCT
NM_153716.1       TCACCTATATCTACTTTATTCAACGAAGATTTCAACAAGAAGCATTTTAGAGCAGGGGCT
NM_033108.2       TCACCTATATCTACTTTATTCAACGAAGATTTCAACAAGAAGCATTTTAGAGCAGGGGCT
                  ************************************************************

HsCD00329533      AACATGGAGAATCATAATTCTGCCTTAGCTGCTGAAGCTAGTGAAGAAAGTTTATTTTCA
NM_153716.1       AACATGGAGAATCATAATTCTGCCTTAGCTGCTGAAGCTAGTGAAGAAAGTTTATTTTCA
NM_033108.2       AACATGGAGAATCATAATTCTGCCTTAGCTGCTGAAGCTAGTGAAGAAAGTTTATTTTCA
                  ************************************************************

HsCD00329533      GCCTCTAAAAATTTAAATATGCCTCTAACAAGGGAATCTTCTGTCAGACAGATAATTGCA
NM_153716.1       GCCTCTAAAAATTTAAATATGCCTCTAACAAGGGAATCTTCTGTCAGACAGATAATTGCA
NM_033108.2       GCCTCTAAAAATTTAAATATGCCTCTAACAAGGGAATCTTCTGTCAGACAGATAATTGCA
                  ************************************************************

HsCD00329533      AATTCATCTGTCCCCATTAGAAGTGGTTTCCCTCCTCCTTCACCTTCAACCTCAGTTGGA
NM_153716.1       AATTCATCTGTCCCCATTAGAAGTGGTTTCCCTCCTCCTTCACCTTCAACCTCAGTTGGA
NM_033108.2       AATTCATCTGTCCCCATTAGAAGTGGTTTCCCTCCTCCTTCACCTTCAACCTCAGTTGGA
                  ************************************************************

HsCD00329533      CCATCAGAACAAATTGCAACAGATCAACATGCTATTTTAAATCAGTTGACCACTATTCAT
NM_153716.1       CCATCAGAACAAATTGCAACAGATCAACATGCTATTTTAAATCAGTTGACCACTATTCAT
NM_033108.2       CCATCAGAACAAATTGCAACAGATCAACATGCTATTTTAAATCAGTTGACCACTATTCAT
                  ************************************************************

HsCD00329533      ATGCACTCTCATAGTACCTACATGCAAGCAAGGGGCCACATTGTGAATTTTATTACAACC
NM_153716.1       ATGCACTCTCATAGTACCTACATGCAAGCAAGGGGCCACATTGTGAATTTTATTACAACC
NM_033108.2       ATGCACTCTCATAGTACCTACATGCAAGCAAGGGGCCACATTGTGAATTTTATTACAACC
                  ************************************************************

HsCD00329533      ACAACTTCTCAATACCACATCATATCTCCCTTACAAAATGGTTATTTTGGGCTGACAGTG
NM_153716.1       ACAACTTCTCAATACCACATCATATCTCCCTTACAAAATGGTTATTTTGGGCTGACAGTG
NM_033108.2       ACAACTTCTCAATACCACATCATATCTCCCTTACAAAATGGTTATTTTGGGCTGACAGTG
                  ************************************************************

HsCD00329533      GAACCATCTGCTGTTCCCACACGATATCCTCTGGTATCAGTCAATGAGGCTCCATATCGT
NM_153716.1       GAACCATCTGCTGTTCCCACACGATATCCTCTGGTATCAGTCAATGAGGCTCCATATCGT
NM_033108.2       GAACCATCTGCTGTTCCCACACGATATCCTCTGGTATCAGTCAATGAGGCTCCATATCGT
                  ************************************************************

HsCD00329533      AACATGCTACCAGCAGGCAACCCGTGGTTGCAAATGCCTACGATCGCTGATAGATCAGCT
NM_153716.1       AACATGCTACCAGCAGGCAACCCGTGGTTGCAAATGCCTACGATCGCTGATAGATCAGCT
NM_033108.2       AACATGCTACCAGCAGGCAACCCGTGGTTGCAAATGCCTACGATCGCTGATAGATCAGCT
                  ************************************************************

HsCD00329533      GCCCCTCATTCCAGGCTAGCTCTTCAACCATCACCACTGGACAAATATCACCCTAATTAC
NM_153716.1       GCCCCTCATTCCAGGCTAGCTCTTCAACCATCACCACTGGACAAATATCACCCTAATTAC
NM_033108.2       GCCCCTCATTCCAGGCTAGCTCTTCAACCATCACCACTGGACAAATATCACCCTAATTAC
                  ************************************************************

HsCD00329533      AACTTG
NM_153716.1       AACTGA
NM_033108.2       AACTGA
                  **** .

Protein

SMART domain : HSF : heat shock factor
UniProt : Q96LI6
HPRD : 11850

Clone

Cloning Information : 268423
HIP Master Clone ID : 194935
Original Clone ID : FLH268423.01L

Organism


TAX_ID : 9606
Species Specific ID: 86614

Genome

Chromosome : Y
Map Location : Yq11.222
Ensembl : ENSG00000172468

Reagents

Labome : HSFY1-antibody