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ZNF670 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF670
Gene Name:  zinc finger protein 670
Sequence             
Original Clone ID: FLH268428.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329537

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1170nts         Open reading frame : 1 to 1170
Reference Sequence Alignment

ATGGATTCAGTGTCATTTGAAGATGTGGCTGTGGCCTTTACTCAGGAGGAGTGGGCTTTG
CTGGATCCTTCTCAAAAGAATCTCTACAGAGATGTGATGCAAGAAATCTTCAGGAACCTG
GCTTCTGTAGGAAACAAATCAGAAGACCAGAATATCCAAGATGACTTCAAAAATCCTGGG
AGAAATCTAAGCAGTCATGTGGTAGAGAGACTGTTTGAAATTAAAGAAGGCAGTCAATAT
GGAGAAACCTTCAGCCAGGATTCAAATTTGAATCTGAATAAGAAAGTTTCTACTGGAGTA
AAGCCATGTGAATGCAGTGTGTGTGGAAAAGTCTTCATATGTCATTCAGCCCTTCATAGG
CACATCCTGTCTCACATTGGAAACAAACTATTTGAGTGTGAGGAATGTCCAGAGAAGTTA
TATCATTGCAAACAATGTGGGAAAGCCTTTATCTCTCTCACCAGTGTTGACAGACACATG
GTAACACACACTAGTAATGGGCCTTATAAGGGTCCAGTGTATGAGAAGCCTTTTGATTTT
CCTAGTGTATTTCAAATGCCTCAGAGCACTTACACTGGAGAGAAAACATATAAATGTAAA
CATTGTGATAAAGCCTTCAATTATTCAAGTTATCTTCGTGAACATGAAAGAACTCATACT
GGAGAGAAACCCTATGCATGTAAGAAATGTGGTAAATCATTCACTTTTTCCAGTTCTCTT
CGCCAACATGAAAGATCTCATACTGGAGAGAAACCCTATGAATGTAAGGAATGTGGCAAA
GCCTTCAGTCGTTCCACTTACTTGGGAATACATGAAAGAACGCATACTGGAGAAAAACCC
TATGAATGTATAAAATGTGGCAAAGCCTTTAGATGTTCCAGAGTCCTCAGAGTCCATGAA
AGGACTCACAGTGGAGAAAAGCCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAATAT
TCTAGTAACCTATGTGAGCATGAAAGAACTCACACTGGAGTGAAACCTTATGGATGTAAG
GAATGTGGTAAGTCGTTTACTTCTTCCAGTGCCCTTCGAAGCCATGAAAGGACTCATACT
GGAGAAAAACCCTATGAATGTAAGAAATGTGGTAAAGCCTTCAGTTGTTCCAGTTCCCTT
CGAAAGCATGAAAGAGCTTATATGTGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1334
Start on reference sequence 165
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF670
Symbol Nomenclature : ZNF670
Full Nomenclature : zinc finger protein 670
GENEID : 93474
HGNC : 28167
Vega : OTTHUMG00000040868
Target GenBank: BC005360

 

Reference Sequence Alignment

  

HsCD00329537        ATGGATTCAGTGTCATTTGAAGATGTGGCTGTGGCCTTTACTCAGGAGGAGTGGGCTTTG
NM_001204220.1      ATGGATTCAGTGTCATTTGAAGATGTGGCTGTGGCCTTTACTCAGGAGGAGTGGGCTTTG
NM_033213.4         ATGGATTCAGTGTCATTTGAAGATGTGGCTGTGGCCTTTACTCAGGAGGAGTGGGCTTTG
                    ************************************************************

HsCD00329537        CTGGATCCTTCTCAAAAGAATCTCTACAGAGATGTGATGCAAGAAATCTTCAGGAACCTG
NM_001204220.1      CTGGATCCTTCTCAAAAGAATCTCTACAGAGATGTGATGCAAGAAATCTTCAGGAACCTG
NM_033213.4         CTGGATCCTTCTCAAAAGAATCTCTACAGAGATGTGATGCAAGAAATCTTCAGGAACCTG
                    ************************************************************

HsCD00329537        GCTTCTGTAGGAAACAAATCAGAAGACCAGAATATCCAAGATGACTTCAAAAATCCTGGG
NM_001204220.1      GCTTCTGTAGGAAACAAATCAGAAGACCAGAATATCCAAGATGACTTCAAAAATCCTGGG
NM_033213.4         GCTTCTGTAGGAAACAAATCAGAAGACCAGAATATCCAAGATGACTTCAAAAATCCTGGG
                    ************************************************************

HsCD00329537        AGAAATCTAAGCAGTCATGTGGTAGAGAGACTGTTTGAAATTAAAGAAGGCAGTCAATAT
NM_001204220.1      AGAAATCTAAGTCATGTG---GTAGAGAGACTGTTTGAAATTAAAGAAGGCAGTCAATAT
NM_033213.4         AGAAATCTAAGCAGTCATGTGGTAGAGAGACTGTTTGAAATTAAAGAAGGCAGTCAATAT
                    *********** ..* :    ***************************************

HsCD00329537        GGAGAAACCTTCAGCCAGGATTCAAATTTGAATCTGAATAAGAAAGTTTCTACTGGAGTA
NM_001204220.1      GGAGAAACCTTCAGCCAGGATTCAAATTTGAATCTGAATAAGAAAGTTTCTACTGGAGTA
NM_033213.4         GGAGAAACCTTCAGCCAGGATTCAAATTTGAATCTGAATAAGAAAGTTTCTACTGGAGTA
                    ************************************************************

HsCD00329537        AAGCCATGTGAATGCAGTGTGTGTGGAAAAGTCTTCATATGTCATTCAGCCCTTCATAGG
NM_001204220.1      AAGCCATGTGAATGCAGTGTGTGTGGAAAAGTCTTCATATGTCATTCAGCCCTTCATAGG
NM_033213.4         AAGCCATGTGAATGCAGTGTGTGTGGAAAAGTCTTCATATGTCATTCAGCCCTTCATAGG
                    ************************************************************

HsCD00329537        CACATCCTGTCTCACATTGGAAACAAACTATTTGAGTGTGAGGAATGTCCAGAGAAGTTA
NM_001204220.1      CACATCCTGTCTCACATTGGAAACAAACTATTTGAGTGTGAGGAATGTCCAGAGAAGTTA
NM_033213.4         CACATCCTGTCTCACATTGGAAACAAACTATTTGAGTGTGAGGAATGTCCAGAGAAGTTA
                    ************************************************************

HsCD00329537        TATCATTGCAAACAATGTGGGAAAGCCTTTATCTCTCTCACCAGTGTTGACAGACACATG
NM_001204220.1      TATCATTGCAAACAATGTGGGAAAGCCTTTATCTCTCTCACCAGTGTTGACAGACACATG
NM_033213.4         TATCATTGCAAACAATGTGGGAAAGCCTTTATCTCTCTCACCAGTGTTGACAGACACATG
                    ************************************************************

HsCD00329537        GTAACACACACTAGTAATGGGCCTTATAAGGGTCCAGTGTATGAGAAGCCTTTTGATTTT
NM_001204220.1      GTAACACACACTAGTAATGGGCCTTATAAGGGTCCAGTGTATGAGAAGCCTTTTGATTTT
NM_033213.4         GTAACACACACTAGTAATGGGCCTTATAAGGGTCCAGTGTATGAGAAGCCTTTTGATTTT
                    ************************************************************

HsCD00329537        CCTAGTGTATTTCAAATGCCTCAGAGCACTTACACTGGAGAGAAAACATATAAATGTAAA
NM_001204220.1      CCTAGTGTATTTCAAATGCCTCAGAGCACTTACACTGGAGAGAAAACATATAAATGTAAA
NM_033213.4         CCTAGTGTATTTCAAATGCCTCAGAGCACTTACACTGGAGAGAAAACATATAAATGTAAA
                    ************************************************************

HsCD00329537        CATTGTGATAAAGCCTTCAATTATTCAAGTTATCTTCGTGAACATGAAAGAACTCATACT
NM_001204220.1      CATTGTGATAAAGCCTTCAATTATTCAAGTTATCTTCGTGAACATGAAAGAACTCATACT
NM_033213.4         CATTGTGATAAAGCCTTCAATTATTCAAGTTATCTTCGTGAACATGAAAGAACTCATACT
                    ************************************************************

HsCD00329537        GGAGAGAAACCCTATGCATGTAAGAAATGTGGTAAATCATTCACTTTTTCCAGTTCTCTT
NM_001204220.1      GGAGAGAAACCCTATGCATGTAAGAAATGTGGTAAATCATTCACTTTTTCCAGTTCTCTT
NM_033213.4         GGAGAGAAACCCTATGCATGTAAGAAATGTGGTAAATCATTCACTTTTTCCAGTTCTCTT
                    ************************************************************

HsCD00329537        CGCCAACATGAAAGATCTCATACTGGAGAGAAACCCTATGAATGTAAGGAATGTGGCAAA
NM_001204220.1      CGCCAACATGAAAGATCTCATACTGGAGAGAAACCCTATGAATGTAAGGAATGTGGCAAA
NM_033213.4         CGCCAACATGAAAGATCTCATACTGGAGAGAAACCCTATGAATGTAAGGAATGTGGCAAA
                    ************************************************************

HsCD00329537        GCCTTCAGTCGTTCCACTTACTTGGGAATACATGAAAGAACGCATACTGGAGAAAAACCC
NM_001204220.1      GCCTTCAGTCGTTCCACTTACTTGGGAATACATGAAAGAACGCATACTGGAGAAAAACCC
NM_033213.4         GCCTTCAGTCGTTCCACTTACTTGGGAATACATGAAAGAACGCATACTGGAGAAAAACCC
                    ************************************************************

HsCD00329537        TATGAATGTATAAAATGTGGCAAAGCCTTTAGATGTTCCAGAGTCCTCAGAGTCCATGAA
NM_001204220.1      TATGAATGTATAAAATGTGGCAAAGCCTTTAGATGTTCCAGAGTCCTCAGAGTCCATGAA
NM_033213.4         TATGAATGTATAAAATGTGGCAAAGCCTTTAGATGTTCCAGAGTCCTCAGAGTCCATGAA
                    ************************************************************

HsCD00329537        AGGACTCACAGTGGAGAAAAGCCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAATAT
NM_001204220.1      AGGACTCACAGTGGAGAAAAGCCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAATAT
NM_033213.4         AGGACTCACAGTGGAGAAAAGCCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAATAT
                    ************************************************************

HsCD00329537        TCTAGTAACCTATGTGAGCATGAAAGAACTCACACTGGAGTGAAACCTTATGGATGTAAG
NM_001204220.1      TCTAGTAACCTATGTGAGCATGAAAGAACTCACACTGGAGTGAAACCTTATGGATGTAAG
NM_033213.4         TCTAGTAACCTATGTGAGCATGAAAGAACTCACACTGGAGTGAAACCTTATGGATGTAAG
                    ************************************************************

HsCD00329537        GAATGTGGTAAGTCGTTTACTTCTTCCAGTGCCCTTCGAAGCCATGAAAGGACTCATACT
NM_001204220.1      GAATGTGGTAAGTCGTTTACTTCTTCCAGTGCCCTTCGAAGCCATGAAAGGACTCATACT
NM_033213.4         GAATGTGGTAAGTCGTTTACTTCTTCCAGTGCCCTTCGAAGCCATGAAAGGACTCATACT
                    ************************************************************

HsCD00329537        GGAGAAAAACCCTATGAATGTAAGAAATGTGGTAAAGCCTTCAGTTGTTCCAGTTCCCTT
NM_001204220.1      GGAGAAAAACCCTATGAATGTAAGAAATGTGGTAAAGCCTTCAGTTGTTCCAGTTCCCTT
NM_033213.4         GGAGAAAAACCCTATGAATGTAAGAAATGTGGTAAAGCCTTCAGTTGTTCCAGTTCCCTT
                    ************************************************************

HsCD00329537        CGAAAGCATGAAAGAGCTTATATGTGGTTG
NM_001204220.1      CGAAAGCATGAAAGAGCTTATATGTGGTAA
NM_033213.4         CGAAAGCATGAAAGAGCTTATATGTGGTAA
                    ****************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9BS34
HPRD : 11305

Clone

Cloning Information : 268428
HIP Master Clone ID : 193431
Original Clone ID : FLH268428.01L

Organism


TAX_ID : 9606
Species Specific ID: 93474

Genome

Chromosome : 1
Map Location : 1q44

Reagents

Labome : ZNF670-antibody