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SAP18 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SAP18
Gene Name:  Sin3A-associated protein, 18kDa
Sequence             
Original Clone ID: FLH268436.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329543

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 462nts         Open reading frame : 1 to 462
Reference Sequence Alignment

ATGGCGGTGGAGTCGCGCGTTACCCAGGAGGAAATTAAGAAGGAGCCAGAGAAACCGATC
GACCGCGAGAAGACATGCCCACTGTTGCTACGGGTCTTCACCACCAATAACGGCCGCCAC
CACCGAATGGACGAGTTCTCCCGGGGAAATGTACCGTCCAGCGAGTTGCAGATCTACACT
TGGATGGATGCAACTTTGAAAGAACTGACAAGCTTAGTAAAAGAAGTCTACCCAGAAGCT
AGAAAGAAGGGCACTCACTTCAATTTTGCAATCGTTTTTACAGATGTTAAAAGACCTGGC
TATCGAGTTAAGGAAATTGGCAGCACCATGTCTGGCAGAAAGGGGACTGATGATTCCATG
ACCCTGCAGTCGCAGAAGTTCCAGATAGGAGATTACTTGGACATAGCAATTACCCCTCCA
AATCGGGCACCACCTACTTCAGGGCGCATGAGACCATATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 526
Start on reference sequence 65
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SAP18
Symbol Nomenclature : SAP18
SYNONYM : 2HOR0202; SAP18P
Designation : 18 kDa Sin3-associated polypeptide|cell growth inhibiting protein 38|cell growth-inhibiting gene 38 protein|cell growth-inhibiting protein 38|histone deacetlyase complex subunit SAP18|histone deacetylase complex subunit SAP18|sin3-associated polypeptide p18|sin3-associated polypeptide, 18 kDa|sin3-associated polypeptide, p18
Full Nomenclature : Sin3A-associated protein, 18kDa
GENEID : 10284
Locus Tag : GIG38
HGNC : 10530
MIM : 602949
Vega : OTTHUMG00000016535
Target GenBank: BC030836

 

Reference Sequence Alignment

  

HsCD00329543      ---------------------------------------------------------ATG
NM_005870.4       ATGCTCGCTGCAGGGGTCGGAGGTCAGGGCGAGCGTCTCGCAGGCCGTAGGAGGAAGATG
                                                                           ***

HsCD00329543      GCGGTGGAGTCGCGCGTTACCCAGGAGGAAATTAAGAAGGAGCCAGAGAAACCGATCGAC
NM_005870.4       GCGGTGGAGTCGCGCGTTACCCAGGAGGAAATTAAGAAGGAGCCAGAGAAACCGATCGAC
                  ************************************************************

HsCD00329543      CGCGAGAAGACATGCCCACTGTTGCTACGGGTCTTCACCACCAATAACGGCCGCCACCAC
NM_005870.4       CGCGAGAAGACATGCCCACTGTTGCTACGGGTCTTCACCACCAATAACGGCCGCCACCAC
                  ************************************************************

HsCD00329543      CGAATGGACGAGTTCTCCCGGGGAAATGTACCGTCCAGCGAGTTGCAGATCTACACTTGG
NM_005870.4       CGAATGGACGAGTTCTCCCGGGGAAATGTACCGTCCAGCGAGTTGCAGATCTACACTTGG
                  ************************************************************

HsCD00329543      ATGGATGCAACTTTGAAAGAACTGACAAGCTTAGTAAAAGAAGTCTACCCAGAAGCTAGA
NM_005870.4       ATGGATGCAACCTTGAAAGAACTGACAAGCTTAGTAAAAGAAGTCTACCCAGAAGCTAGA
                  *********** ************************************************

HsCD00329543      AAGAAGGGCACTCACTTCAATTTTGCAATCGTTTTTACAGATGTTAAAAGACCTGGCTAT
NM_005870.4       AAGAAGGGCACTCACTTCAATTTTGCAATCGTTTTTACAGATGTTAAAAGACCTGGCTAT
                  ************************************************************

HsCD00329543      CGAGTTAAGGAAATTGGCAGCACCATGTCTGGCAGAAAGGGGACTGATGATTCCATGACC
NM_005870.4       CGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGAAAGGGGACTGATGATTCCATGACC
                  ***********.************************************************

HsCD00329543      CTGCAGTCGCAGAAGTTCCAGATAGGAGATTACTTGGACATAGCAATTACCCCTCCAAAT
NM_005870.4       CTGCAGTCGCAGAAGTTCCAGATAGGAGATTACTTGGACATAGCAATTACCCCTCCAAAT
                  ************************************************************

HsCD00329543      CGGGCACCACCTACTTCAGGGCGCATGAGACCATATTTG
NM_005870.4       CGGGCACCACCTCCTTCAGGGCGCATGAGACCATATTAA
                  ************.************************:.

Pathways

NCI : Hedgehog signaling events mediated by Gli proteins
NCI : Regulation of Telomerase
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Signaling events mediated by HDAC Class I
Reactome : Epigenetic regulation of gene expression
Reactome : Gene Expression
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
WikiPathway : Hedgehog Signaling Pathway

Protein

UniProt : O00422
HPRD : 04256

Clone

Cloning Information : 268436
HIP Master Clone ID : 193448
Original Clone ID : FLH268436.01L

Organism


TAX_ID : 9606
Species Specific ID: 10284

Genome

Chromosome : 13
Map Location : 13q12.11
Ensembl : ENSG00000150459

Reagents

Labome : SAP18-antibody