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FLJ11011 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  UBE2W
Gene Name:  ubiquitin-conjugating enzyme E2W (putative)
Sequence             
Original Clone ID: FLH268437.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329544

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 480nts         Open reading frame : 1 to 480
Reference Sequence Alignment

ATGGCGTCAATGCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCATCT
CCTGGAATGACCTTAAATGAGAAGAGTGCTCAAAATTCAATTACACAGTGGATTGTAGAC
ATGGAAAGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
AAAACAAAATGGTGGTATCATGAACTGAAATCTGCCTTCATACTATCTATTACGGATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 487
Start on reference sequence 8
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2W
Symbol Nomenclature : UBE2W
SYNONYM : UBC-16; UBC16
Designation : N-terminus-conjugating E2|ubiquitin carrier protein W|ubiquitin-conjugating enzyme 16|ubiquitin-conjugating enzyme 2W|ubiquitin-conjugating enzyme E2 W|ubiquitin-protein ligase W
Full Nomenclature : ubiquitin-conjugating enzyme E2W (putative)
GENEID : 55284
HGNC : 25616
MIM : 614277
Vega : OTTHUMG00000164517
Target GenBank: BC010900

 

Reference Sequence Alignment

  

HsCD00329544        ------------------------------------------------------------
NM_001271015.1      ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
NM_001001481.2      ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
NM_018299.4         ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
                                                                                

HsCD00329544        ---------------------------ATGGCGTCAATG---------------------
NM_001271015.1      CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAATG---------------------
NM_001001481.2      CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAATGCAGACCACAGGAAGGAGGGTG
NM_018299.4         CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAAT----------------------
                                               ***********                      

HsCD00329544        ------------CAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCATCT
NM_001271015.1      ------------CAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
NM_001001481.2      GAAGTATGGTTTCCAAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
NM_018299.4         -----------GCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
                                *..****************************************** **

HsCD00329544        CCTGGAATGACCTTAAATGAGAAGAGTGCTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_001271015.1      CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_001001481.2      CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_018299.4         CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
                    **************************** *******************************

HsCD00329544        ATGGAAAGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_001271015.1      ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_001001481.2      ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_018299.4         ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
                    ******.*****************************************************

HsCD00329544        AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_001271015.1      AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_001001481.2      AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_018299.4         AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
                    ************************************************************

HsCD00329544        CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_001271015.1      CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_001001481.2      CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_018299.4         CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
                    ************************************************************

HsCD00329544        CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_001271015.1      CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_001001481.2      CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_018299.4         CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
                    ************************************************************

HsCD00329544        GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_001271015.1      GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_001001481.2      GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_018299.4         GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
                    ************************************************************

HsCD00329544        AAAACAAAATGGTGGTATCATGAACTGAAATCTGCCTTCATACTATCTATTACGGATTTG
NM_001271015.1      AAAACAAAATGGTGGTATCATGAACTGAAATCTGCCTTCATACTATCTATTACGGATTGA
NM_001001481.2      AAAACAAAATGGTGGTATCATGATGATACTTGTTGA------------------------
NM_018299.4         AAAACAAAATGGTGGTATCATGATGATACTTGTTGA------------------------
                    ***********************: : *.:* *  .                        

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Cloning Information : 268437
HIP Master Clone ID : 193450
Original Clone ID : FLH268437.01L

Organism


TAX_ID : 9606
Species Specific ID: 55284

Genome

Chromosome : 8
Map Location : 8q21.11
Ensembl : ENSG00000104343

Reagents

Labome : UBE2W-antibody