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RFP2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIM13
Gene Name:  tripartite motif containing 13
Sequence             
Original Clone ID: FLH269052.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329554

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1224nts         Open reading frame : 1 to 1224
Reference Sequence Alignment

ATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTTGATGATCCA
CGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATCTTAGAAGGG
AGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGCCGTAAGGAA
ACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGTATTGTGGAA
AAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACACTTGGGGCAG
CCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGTGCTACTCGT
GGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAGGAAAGGGAT
GCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCTCTTTCTCGC
TTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAAGATTCAGAT
AAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAGAATGAAATT
CTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCAGAGATCAAC
AAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAGGCTTTCAAA
GATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAGAAAATCAAA
GTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTAATGAAGAAC
TTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTTTCTTTGCCT
CAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTATTTTTGCTA
ATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAATGGTCATTA
TTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTATCTGACTAAA
ACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGATGGGTTTTTC
ATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTGGCAGAATTT
GTGTGCAAATATAAACTATTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1498
Start on reference sequence 275
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM13
Symbol Nomenclature : TRIM13
SYNONYM : CAR; DLEU5; LEU5; RFP2; RNF77
Designation : B-cell chronic lymphocytic leukemia tumor suppressor Leu5|CLL-associated RING finger|E3 ubiquitin-protein ligase TRIM13|RING finger protein 77|leukemia-associated protein 5|putative tumor suppressor RFP2|ret finger protein 2|tripartite motif protein 13|tripartite motif-containing 13|tripartite motif-containing protein 13
Full Nomenclature : tripartite motif containing 13
GENEID : 10206
HGNC : 9976
MIM : 605661
Vega : OTTHUMG00000016926
Target GenBank: BC003579

 

Reference Sequence Alignment

  

HsCD00329554        ---------ATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTT
NM_052811.3         ---------ATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTT
NM_001007278.2      ATGGATGTGATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTT
NM_005798.4         ---------ATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTT
NM_213590.2         ---------ATGGAGCTGCTTGAAGAAGATCTCACATGCCCTATTTGTTGTAGTCTGTTT
                             ***************************************************

HsCD00329554        GATGATCCACGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATC
NM_052811.3         GATGATCCACGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATC
NM_001007278.2      GATGATCCACGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATC
NM_005798.4         GATGATCCACGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATC
NM_213590.2         GATGATCCACGGGTTTTGCCTTGCTCCCACAACTTCTGCAAAAAATGCTTAGAAGGTATC
                    ************************************************************

HsCD00329554        TTAGAAGGGAGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGC
NM_052811.3         TTAGAAGGGAGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGC
NM_001007278.2      TTAGAAGGGAGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGC
NM_005798.4         TTAGAAGGGAGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGC
NM_213590.2         TTAGAAGGGAGTGTGCGGAATTCCTTGTGGAGACCAGCTCCATTCAAGTGTCCTACATGC
                    ************************************************************

HsCD00329554        CGTAAGGAAACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGT
NM_052811.3         CGTAAGGAAACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGT
NM_001007278.2      CGTAAGGAAACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGT
NM_005798.4         CGTAAGGAAACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGT
NM_213590.2         CGTAAGGAAACTTCAGCTACTGGAATTAATAGCCTGCAGGTTAATTACTCCCTGAAGGGT
                    ************************************************************

HsCD00329554        ATTGTGGAAAAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACAC
NM_052811.3         ATTGTGGAAAAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACAC
NM_001007278.2      ATTGTGGAAAAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACAC
NM_005798.4         ATTGTGGAAAAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACAC
NM_213590.2         ATTGTGGAAAAGTATAACAAGATCAAGATCTCTCCCAAAATGCCAGTATGCAAAGGACAC
                    ************************************************************

HsCD00329554        TTGGGGCAGCCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGT
NM_052811.3         TTGGGGCAGCCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGT
NM_001007278.2      TTGGGGCAGCCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGT
NM_005798.4         TTGGGGCAGCCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGT
NM_213590.2         TTGGGGCAGCCTCTCAACATTTTCTGCCTGACTGATATGCAGCTGATTTGTGGGATCTGT
                    ************************************************************

HsCD00329554        GCTACTCGTGGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAG
NM_052811.3         GCTACTCGTGGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAG
NM_001007278.2      GCTACTCGTGGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAG
NM_005798.4         GCTACTCGTGGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAG
NM_213590.2         GCTACTCGTGGGGAGCACACCAAACATGTCTTCTGTTCTATTGAAGATGCCTATGCTCAG
                    ************************************************************

HsCD00329554        GAAAGGGATGCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCT
NM_052811.3         GAAAGGGATGCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCT
NM_001007278.2      GAAAGGGATGCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCT
NM_005798.4         GAAAGGGATGCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCT
NM_213590.2         GAAAGGGATGCCTTTGAGTCCCTCTTCCAGAGCTTTGAGACCTGGCGTCGGGGAGATGCT
                    ************************************************************

HsCD00329554        CTTTCTCGCTTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAA
NM_052811.3         CTTTCTCGCTTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAA
NM_001007278.2      CTTTCTCGCTTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAA
NM_005798.4         CTTTCTCGCTTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAA
NM_213590.2         CTTTCTCGCTTGGATACCTTGGAAACTAGTAAGAGGAAATCCCTACAGTTACTGACTAAA
                    ************************************************************

HsCD00329554        GATTCAGATAAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAG
NM_052811.3         GATTCAGATAAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAG
NM_001007278.2      GATTCAGATAAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAG
NM_005798.4         GATTCAGATAAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAG
NM_213590.2         GATTCAGATAAAGTGAAGGAATTTTTTGAGAAGTTACAACACACACTGGATCAAAAGAAG
                    ************************************************************

HsCD00329554        AATGAAATTCTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCA
NM_052811.3         AATGAAATTCTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCA
NM_001007278.2      AATGAAATTCTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCA
NM_005798.4         AATGAAATTCTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCA
NM_213590.2         AATGAAATTCTGTCTGACTTTGAGACCATGAAACTTGCTGTTATGCAAGCATATGACCCA
                    ************************************************************

HsCD00329554        GAGATCAACAAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAG
NM_052811.3         GAGATCAACAAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAG
NM_001007278.2      GAGATCAACAAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAG
NM_005798.4         GAGATCAACAAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAG
NM_213590.2         GAGATCAACAAACTCAACACCATCTTGCAGGAGCAACGGATGGCCTTTAACATTGCTGAG
                    ************************************************************

HsCD00329554        GCTTTCAAAGATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAG
NM_052811.3         GCTTTCAAAGATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAG
NM_001007278.2      GCTTTCAAAGATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAG
NM_005798.4         GCTTTCAAAGATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAG
NM_213590.2         GCTTTCAAAGATGTGTCAGAACCCATTGTATTTCTGCAACAGATGCAGGAGTTTAGAGAG
                    ************************************************************

HsCD00329554        AAAATCAAAGTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTA
NM_052811.3         AAAATCAAAGTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTA
NM_001007278.2      AAAATCAAAGTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTA
NM_005798.4         AAAATCAAAGTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTA
NM_213590.2         AAAATCAAAGTAATCAAGGAAACTCCTTTACCTCCCTCTAATTTGCCTGCAAGCCCTTTA
                    ************************************************************

HsCD00329554        ATGAAGAACTTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTT
NM_052811.3         ATGAAGAACTTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTT
NM_001007278.2      ATGAAGAACTTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTT
NM_005798.4         ATGAAGAACTTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTT
NM_213590.2         ATGAAGAACTTTGATACCAGTCAGTGGGAAGACATAAAACTAGTCGATGTGGATAAACTT
                    ************************************************************

HsCD00329554        TCTTTGCCTCAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTA
NM_052811.3         TCTTTGCCTCAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTA
NM_001007278.2      TCTTTGCCTCAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTA
NM_005798.4         TCTTTGCCTCAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTA
NM_213590.2         TCTTTGCCTCAAGACACTGGCACATTCATTAGCAAGATTCCCTGGAGCTTTTATAAGTTA
                    ************************************************************

HsCD00329554        TTTTTGCTAATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAA
NM_052811.3         TTTTTGCTAATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAA
NM_001007278.2      TTTTTGCTAATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAA
NM_005798.4         TTTTTGCTAATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAA
NM_213590.2         TTTTTGCTAATCCTTCTGCTTGGCCTTGTCATTGTCTTTGGTCCTACCATGTTCCTAGAA
                    ************************************************************

HsCD00329554        TGGTCATTATTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTAT
NM_052811.3         TGGTCATTATTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTAT
NM_001007278.2      TGGTCATTATTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTAT
NM_005798.4         TGGTCATTATTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTAT
NM_213590.2         TGGTCATTATTTGATGACCTGGCAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTAT
                    ************************************************************

HsCD00329554        CTGACTAAAACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGAT
NM_052811.3         CTGACTAAAACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGAT
NM_001007278.2      CTGACTAAAACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGAT
NM_005798.4         CTGACTAAAACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGAT
NM_213590.2         CTGACTAAAACAGCCGATTTCATAGAACAATCAGTTTTTTACTGGGAACAGGTGACAGAT
                    ************************************************************

HsCD00329554        GGGTTTTTCATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTG
NM_052811.3         GGGTTTTTCATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTG
NM_001007278.2      GGGTTTTTCATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTG
NM_005798.4         GGGTTTTTCATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTG
NM_213590.2         GGGTTTTTCATTTTCAATGAAAGATTCAAGAATTTTACTTTGGTGGTACTGAACAATGTG
                    ************************************************************

HsCD00329554        GCAGAATTTGTGTGCAAATATAAACTATTATTG
NM_052811.3         GCAGAATTTGTGTGCAAATATAAACTATTATAA
NM_001007278.2      GCAGAATTTGTGTGCAAATATAAACTATTATAA
NM_005798.4         GCAGAATTTGTGTGCAAATATAAACTATTATAA
NM_213590.2         GCAGAATTTGTGTGCAAATATAAACTATTATAA
                    *******************************:.

Protein

SMART domain : BBOX : B-Box-type zinc finger
SMART domain : RING : Ring finger
UniProt : Q8N5I3
UniProt : O60858
HPRD : 10414

Clone

Cloning Information : 269052
HIP Master Clone ID : 162579
Original Clone ID : FLH269052.01L

Organism


TAX_ID : 9606
Species Specific ID: 10206

Genome

Chromosome : 13
Map Location : 13q14
Ensembl : ENSG00000204977

Reagents

Labome : RFP2-antibody