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NRBP (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NRBP1
Gene Name:  nuclear receptor binding protein 1
Sequence             
Original Clone ID: FLH269054.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329556

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1608nts         Open reading frame : 1 to 1608
Reference Sequence Alignment

ATGTCGGAGGGGGAGTCCCAGACAGTACTTAGCAGTGGCTCAGACCCAAAGGTAGAATCC
TCATCTTCAGCTCCTGGCCTGACATCAGTGTCACCTCCTGTGACCTCCACAACCTCAGCT
GCTTCCCCAGAGGAAGAAGAAGAAAGTGAAGATGAGTCTGAGATTTTGGAAGAGTCGCCC
TGTGGGCGCTGGCAGAAGAGGCGAGAAGAGGTGAATCAACGGAATGTACCAGGTATTGAC
AGTGCATACCTGGCCATGGATACAGAGGAAGGTGTAGAGGTTGTGTGGAATGAGGTACAG
TTCTCTGAACGCAAGAACTACAAGCTGCAGGAGGAAAAGGTTCGTGCTGTGTTTGATAAT
CTGATTCAATTGGAGCATCTTAACATTGTTAAGTTTCACAAATATTGGGCTGACATTAAA
GAGAACAAGGCCAGGGTCATTTTTATCACAGAATACATGTCATCTGGGAGTCTGAAGCAA
TTTCTGAAGAAGACCAAAAAGAACCACAAGACGATGAATGAAAAGGCATGGAAGCGTTGG
TGCACACAAATCCTCTCTGCCCTAAGCTACCTGCACTCCTGTGACCCCCCCATCATCCAT
GGGAACCTGACCTGTGACACCATCTTCATCCAGCACAACGGACTCATCAAGATTGGCTCT
GTGGCTCCTGACACTATCAACAATCATGTGAAGACTTGTCGAGAAGAGCAGAAGAATCTA
CACTTCTTTGCACCAGAGTATGGAGAAGTCACTAATGTGACAACAGCAGTGGACATCTAC
TCCTTTGGCATGTGTGCACTGGAGATGGCAGTGCTGGAGATTCAGGGCAATGGAGAGTCC
TCATATGTGCCACAGGAAGCCATCAGCAGTGCCATCCAGCTTCTAGAAGACCCATTACAG
AGGGAGTTCATTCAAAAGTGCCTGCAGTCTGAGCCTGCTCGCAGACCAACAGCCAGAGAA
CTTCTGTTCCACCCAGCATTGTTTGAAGTGCCCTCGCTCAAACTCCTTGCGGCCCACTGC
ATTGTGGGACACCAACACATGATCCCAGAGAACGCTCTAGAGGAGATCACCAAAAACATG
GATACTAGTGCCGTACTGGCTGAAATCCCTGCAGGACCAGGAAGAGAACCAGTTCAGACT
TTGTACTCTCAGTCACCAGCTCTGGAATTAGATAAATTCCTTGAAGATGTCAGGAATGGG
ATCTATCCTCTGACAGCCTTTGGGCTGCCTCGGCCCCAGCAGCCACAGCAGGAGGAGGTG
ACATCACCTGTCGTGCCCCCCTCTGTCAAGACTCCGACACCTGAACCAGCTGAGGTGGAG
ACTCGCAAGGTGGTGCTGATGCAGTGCAACATTGAGTCGGTGGAGGAGGGAGTCAAACAC
CACCTGACACTTCTGCTGAAGTTGGAGGACAAACTGAACCGGCACCTGAGCTGTGACCTG
ATGCCAAATGAGAATATCCCCGAGTTGGCGGCTGAGCTGGTGCAGCTGGGCTTCATTAGT
GAGGCTGACCAGAGCCGGTTGACTTCTCTGCTAGAAGAGACCTTGAACAAGTTCAATTTT
GCCAGGAACAGTACCCTCAACTCAGCCGCTGTCACCGTCTCCTCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1722
Start on reference sequence 115
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NRBP1
Symbol Nomenclature : NRBP1
SYNONYM : BCON3; MADM; MUDPNP; NRBP
Designation : multiple domain putative nuclear protein|myeloid leukemia factor 1 adaptor molecule|nuclear receptor-binding protein
Full Nomenclature : nuclear receptor binding protein 1
GENEID : 29959
HGNC : 7993
MIM : 606010
Vega : OTTHUMG00000097789
Target GenBank: BC001221

 

Reference Sequence Alignment

  

HsCD00329556        ATGTCGGAGGGGGAGTCCCAGACAGTACTTAGCAGTGGCTCAGACCCAAAGGTAGAATCC
XM_005264274.1      ATGTCGGAGGGGGAGTCCCAGACAGTACTTAGCAGTGGCTCAGACCCAAAGGTAGAATCC
NM_013392.2         ATGTCGGAGGGGGAGTCCCAGACAGTACTTAGCAGTGGCTCAGACCCAAAGGTAGAATCC
                    ************************************************************

HsCD00329556        TCATCTTCAGCTCCTGGCCTGACATCAGTGTCACCTCCTGTGACCTCCACAACCTCAGCT
XM_005264274.1      TCATCTTCAGCTCCTGGCCTGACATCAGTGTCACCTCCTGTGACCTCCACAACCTCAGCT
NM_013392.2         TCATCTTCAGCTCCTGGCCTGACATCAGTGTCACCTCCTGTGACCTCCACAACCTCAGCT
                    ************************************************************

HsCD00329556        GCTTCCCCAGAGGAAGAAGAAGAAAGTGAAGATGAGTCTGAGATTTTGGAAGAGTCGCCC
XM_005264274.1      GCTTCCCCAGAGGAAGAAGAAGAAAGTGAAGATGAGTCTGAGATTTTGGAAGAGTCGCCC
NM_013392.2         GCTTCCCCAGAGGAAGAAGAAGAAAGTGAAGATGAGTCTGAGATTTTGGAAGAGTCGCCC
                    ************************************************************

HsCD00329556        TGTGGGCGCTGGCAGAAGAGGCGAGAAGAGGTGAATCAACGGAATGTACCAGGTATTGAC
XM_005264274.1      TGTGGGCGCTGGCAGAAGAGGCGAGAAGAGGTGAATCAACGGAATGTACCAGGTATTGAC
NM_013392.2         TGTGGGCGCTGGCAGAAGAGGCGAGAAGAGGTGAATCAACGGAATGTACCAGGTATTGAC
                    ************************************************************

HsCD00329556        AGTGCATACCTGGCCATGGATACAGAGGAAGGTGTAGAGGTTGTGTGGAATGAGGTACAG
XM_005264274.1      AGTGCATACCTGGCCATGGATACAGAGGAAGGTGTAGAGGTTGTGTGGAATGAGGTACAG
NM_013392.2         AGTGCATACCTGGCCATGGATACAGAGGAAGGTGTAGAGGTTGTGTGGAATGAGGTACAG
                    ************************************************************

HsCD00329556        TTCTCTGAACGCAAGAACTACAAGCTGCAGGAGGAAAAGGTTCGTGCTGTGTTTGATAAT
XM_005264274.1      TTCTCTGAACGCAAGAACTACAAGCTGCAGGAGGAAAAGGTTCGTGCTGTGTTTGATAAT
NM_013392.2         TTCTCTGAACGCAAGAACTACAAGCTGCAGGAGGAAAAGGTTCGTGCTGTGTTTGATAAT
                    ************************************************************

HsCD00329556        CTGATTCAATTGGAGCATCTTAACATTGTTAAGTTTCACAAATATTGGGCTGACATTAAA
XM_005264274.1      CTGATTCAATTGGAGCATCTTAACATTGTTAAGTTTCACAAATATTGGGCTGACATTAAA
NM_013392.2         CTGATTCAATTGGAGCATCTTAACATTGTTAAGTTTCACAAATATTGGGCTGACATTAAA
                    ************************************************************

HsCD00329556        GAGAACAAGGCCAGGGTCATTTTTATCACAGAATACATGTCATCTGGGAGTCTGAAGCAA
XM_005264274.1      GAGAACAAGGCCAGGGTCATTTTTATCACAGAATACATGTCATCTGGGAGTCTGAAGCAA
NM_013392.2         GAGAACAAGGCCAGGGTCATTTTTATCACAGAATACATGTCATCTGGGAGTCTGAAGCAA
                    ************************************************************

HsCD00329556        TTTCTGAAGAAGACCAAAAAGAACCACAAGACGATGAATGAAAAGGCATGGAAGCGTTGG
XM_005264274.1      TTTCTGAAGAAGACCAAAAAGAACCACAAGACGATGAATGAAAAGGCATGGAAGCGTTGG
NM_013392.2         TTTCTGAAGAAGACCAAAAAGAACCACAAGACGATGAATGAAAAGGCATGGAAGCGTTGG
                    ************************************************************

HsCD00329556        TGCACACAAATCCTCTCTGCCCTAAGCTACCTGCACTCCTGTGACCCCCCCATCATCCAT
XM_005264274.1      TGCACACAAATCCTCTCTGCCCTAAGCTACCTGCACTCCTGTGACCCCCCCATCATCCAT
NM_013392.2         TGCACACAAATCCTCTCTGCCCTAAGCTACCTGCACTCCTGTGACCCCCCCATCATCCAT
                    ************************************************************

HsCD00329556        GGGAACCTGACCTGTGACACCATCTTCATCCAGCACAACGGACTCATCAAGATTGGCTCT
XM_005264274.1      GGGAACCTGACCTGTGACACCATCTTCATCCAGCACAACGGACTCATCAAGATTGGCTCT
NM_013392.2         GGGAACCTGACCTGTGACACCATCTTCATCCAGCACAACGGACTCATCAAGATTGGCTCT
                    ************************************************************

HsCD00329556        GTGGCTCCTGACACTATCAACAATCATGTGAAGACTTGTCGAGAAGAGCAGAAGAATCTA
XM_005264274.1      GTGGCTCCTGACACTATCAACAATCATGTGAAGACTTGTCGAGAAGAGCAGAAGAATCTA
NM_013392.2         GTGGCTCCTGACACTATCAACAATCATGTGAAGACTTGTCGAGAAGAGCAGAAGAATCTA
                    ************************************************************

HsCD00329556        CACTTCTTTGCACCAGAGTATGGAGAAGTCACTAATGTGACAACAGCAGTGGACATCTAC
XM_005264274.1      CACTTCTTTGCACCAGAGTATGGAGAAGTCACTAATGTGACAACAGCAGTGGACATCTAC
NM_013392.2         CACTTCTTTGCACCAGAGTATGGAGAAGTCACTAATGTGACAACAGCAGTGGACATCTAC
                    ************************************************************

HsCD00329556        TCCTTTGGCATGTGTGCACTGGAGATGGCAGTGCTGGAGATTCAGGGCAATGGAGAGTCC
XM_005264274.1      TCCTTTGGCATGTGTGCACTGGAGATGGCAGTGCTGGAGATTCAGGGCAATGGAGAGTCC
NM_013392.2         TCCTTTGGCATGTGTGCACTGGAGATGGCAGTGCTGGAGATTCAGGGCAATGGAGAGTCC
                    ************************************************************

HsCD00329556        TCATATGTGCCACAGGAAGCCATCAGCAGTGCCATCCAGCTTCTAGAAGACCCATTACAG
XM_005264274.1      TCATATGTGCCACAGGAAGCCATCAGCAGTGCCATCCAGCTTCTAGAAGACCCATTACAG
NM_013392.2         TCATATGTGCCACAGGAAGCCATCAGCAGTGCCATCCAGCTTCTAGAAGACCCATTACAG
                    ************************************************************

HsCD00329556        AGGGAGTTCATTCAAAAGTGCCTGCAGTCTGAGCCTGCTCGCAGACCAACAGCCAGAGAA
XM_005264274.1      AGGGAGTTCATTCAAAAGTGCCTGCAGTCTGAGCCTGCTCGCAGACCAACAGCCAGAGAA
NM_013392.2         AGGGAGTTCATTCAAAAGTGCCTGCAGTCTGAGCCTGCTCGCAGACCAACAGCCAGAGAA
                    ************************************************************

HsCD00329556        CTTCTGTTCCACCCAGCATTGTTTGAAGTGCCCTCGCTCAAACTCCTTGCGGCCCACTGC
XM_005264274.1      CTTCTGTTCCACCCAGCATTGTTTGAAGTGCCCTCGCTCAAACTCCTTGCGGCCCACTGC
NM_013392.2         CTTCTGTTCCACCCAGCATTGTTTGAAGTGCCCTCGCTCAAACTCCTTGCGGCCCACTGC
                    ************************************************************

HsCD00329556        ATTGTGGGACACCAACACATGATCCCAGAGAACGCTCTAGAGGAGATCACCAAAAACATG
XM_005264274.1      ATTGTGGGACACCAACACATGATCCCAGAGAACGCTCTAGAGGAGATCACCAAAAACATG
NM_013392.2         ATTGTGGGACACCAACACATGATCCCAGAGAACGCTCTAGAGGAGATCACCAAAAACATG
                    ************************************************************

HsCD00329556        GATACTAGTGCCGTACTGGCTGAAATCCCTGCAGGACCAGGAAGAGAACCAGTTCAGACT
XM_005264274.1      GATACTAGTGCCGTACTGGCTGAAATCCCTGCAGGACCAGGAAGAGAACCAGTTCAGACT
NM_013392.2         GATACTAGTGCCGTACTGGCTGAAATCCCTGCAGGACCAGGAAGAGAACCAGTTCAGACT
                    ************************************************************

HsCD00329556        TTGTACTCTCAGTCACCAGCTCTGGAATTAGATAAATTCCTTGAAGATGTCAGGAATGGG
XM_005264274.1      TTGTACTCTCAGTCACCAGCTCTGGAATTAGATAAATTCCTTGAAGATGTCAGGAATGGG
NM_013392.2         TTGTACTCTCAGTCACCAGCTCTGGAATTAGATAAATTCCTTGAAGATGTCAGGAATGGG
                    ************************************************************

HsCD00329556        ATCTATCCTCTGACAGCCTTTGGGCTGCCTCGGCCCCAGCAGCCACAGCAGGAGGAGGTG
XM_005264274.1      ATCTATCCTCTGACAGCCTTTGGGCTGCCTCGGCCCCAGCAGCCACAGCAGGAGGAGGTG
NM_013392.2         ATCTATCCTCTGACAGCCTTTGGGCTGCCTCGGCCCCAGCAGCCACAGCAGGAGGAGGTG
                    ************************************************************

HsCD00329556        ACATCACCTGTCGTGCCCCCCTCTGTCAAGACTCCGACACCTGAACCAGCTGAGGTGGAG
XM_005264274.1      ACATCACCTGTCGTGCCCCCCTCTGTCAAGACTCCGACACCTGAACCAGCTGAGGTGGAG
NM_013392.2         ACATCACCTGTCGTGCCCCCCTCTGTCAAGACTCCGACACCTGAACCAGCTGAGGTGGAG
                    ************************************************************

HsCD00329556        ACTCGCAAGGTGGTGCTGATGCAGTGCAACATTGAGTCGGTGGAGGAGGGAGTCAAACAC
XM_005264274.1      ACTCGCAAGGTGGTGCTGATGCAGTGCAACATTGAGTCGGTGGAGGAGGGAGTCAAACAC
NM_013392.2         ACTCGCAAGGTGGTGCTGATGCAGTGCAACATTGAGTCGGTGGAGGAGGGAGTCAAACAC
                    ************************************************************

HsCD00329556        CACCTGACACTTCTGCTGAAGTTGGAGGACAAACTGAACCGGCACCTGAGCTGTGACCTG
XM_005264274.1      CACCTGACACTTCTGCTGAAGTTGGAGGACAAACTGAACCGGCACCTGAGCTGTGACCTG
NM_013392.2         CACCTGACACTTCTGCTGAAGTTGGAGGACAAACTGAACCGGCACCTGAGCTGTGACCTG
                    ************************************************************

HsCD00329556        ATGCCAAATGAGAATATCCCCGAGTTGGCGGCTGAGCTGGTGCAGCTGGGCTTCATTAGT
XM_005264274.1      ATGCCAAATGAGAATATCCCCGAGTTGGCGGCTGAGCTGGTGCAGCTGGGCTTCATTAGT
NM_013392.2         ATGCCAAATGAGAATATCCCCGAGTTGGCGGCTGAGCTGGTGCAGCTGGGCTTCATTAGT
                    ************************************************************

HsCD00329556        GAGGCTGACCAGAGCCGGTTGACTTCTCTGCTAGAAGAGACCTTGAACAAGTTCAATTTT
XM_005264274.1      GAGGCTGACCAGAGCCGGTTGACTTCTCTGCTAGAAGAGACCTTGAACAAGTTCAATTTT
NM_013392.2         GAGGCTGACCAGAGCCGGTTGACTTCTCTGCTAGAAGAGACCTTGAACAAGTTCAATTTT
                    ************************************************************

HsCD00329556        GCCAGGAACAGTACCCTCAACTCAGCCGCTGTCACCGTCTCCTCTTTG
XM_005264274.1      GCCAGGAACAGTACCCTCAACTCAGCCGCTGTCACCGTCTCCTCTTAG
NM_013392.2         GCCAGGAACAGTACCCTCAACTCAGCCGCTGTCACCGTCTCCTCTTAG
                    **********************************************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Nuclear Receptor transcription pathway

Clone

Cloning Information : 269054
HIP Master Clone ID : 162866
Original Clone ID : FLH269054.01L

Organism


TAX_ID : 9606
Species Specific ID: 29959

Genome

Chromosome : 2
Map Location : 2p23
Ensembl : ENSG00000115216

Reagents

Labome : NRBP-antibody