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CBX6 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CBX6
Gene Name:  chromobox homolog 6
Sequence             
Original Clone ID: FLH269057.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329559

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1239nts         Open reading frame : 1 to 1239
Reference Sequence Alignment

ATGGAGCTGTCTGCAGTGGGCGAGCGGGTCTTCGCGGCCGAATCCATCATCAAACGGCGG
ATCCGAAAGGGACGCATCGAGTACCTGGTGAAATGGAAGGGGTGGGCGATCAAGTACAGC
ACTTGGGAGCCCGAGGAGAACATCCTGGACTCGCGGCTCATTGCAGCCTTCGAACAAAAG
GAGAGGGAGCGTGAGCTGTATGGGCCCAAGAAGAGGGGACCCAAACCCAAAACTTTCCTC
CTGAAGGCGCGGGCCCAGGCCGAGGCCCTCCGCATCAGTGATGTGCATTTCTCTGTCAAG
CCGAGCGCCAGTGCCTCCTCGCCCAAGCTGCACTCCAGCGCAGCCGTGCACCGGCTCAAG
AAGGACATCCGCCGCTGCCACCGTATGTCCCGCCGTCCCCTGCCCCGCCCGGACCCGCAG
GGGGGCAGCCCCGGACTGCGCCCGCCCATTTCGCCCTTCTCGGAGACGGTGCGCATCATC
AACCGCAAGGTGAAGCCGCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTG
ATCGACAAGGGCGCTGGCGGCGGGGGCGCCGGGCAGGGGGCCGGGGCGCTGGCCCGCCCC
AAAGTCCCCTCGCGGAACCGCGTTATAGGCAAGAGCAAGAAGTTCAGCGAGAGCGTCCTG
CGTACACAGATCCGCCACATGAAGTTCGGCGCCTTTGCGCTGTACAAGCCTCCGCCCGCC
CCCCTGGTAGCCCCGTCCCCCGGCAAGGCTGAGGCCTCAGCCCCGGGCCCTGGGCTACTT
CTGGCCGCCCCCGCCGCCCCCTACGACGCCCGCAGCTCTGGCTCCTCCGGCTGCCCCTCG
CCTACACCACAGTCCTCTGACCCCGACGACACGCTCCCCAAGCTCCTCCCCGAGACCGTG
AGCCCATCCGCCCCCAGCTGGCGCGAGCCGGAGGTGCTCGACCTGTCCCTCCCTCCCGAG
TCGGCAGCCACCAGCAAGCGGGCACCGCCTGAGGTCACAGCTGCTGCCGGCCCGGCACCT
CCCACGGCCCCTGAGCCCGCCGGTGCCTCCTCCGAGCCCGAGGCTGGGGACTGGCGCCCC
GAGATGTCACCCTGCTCCAATGTGGTCGTCACCGATGTCACCAGCAACCTCCTGACGGTC
ACAATCAAGGAATTCTGCAACCCTGAGGATTTCGAGAAGGTGGCTGCTGGGGTAGCAGGC
GCCGCTGGGGGCGGTGGCAGCATTGGGGCGAGCAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1262
Start on reference sequence 24
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CBX6
Symbol Nomenclature : CBX6
Designation : chromobox protein homolog 6
Full Nomenclature : chromobox homolog 6
GENEID : 23466
Locus Tag : RP3-327J16.5
HGNC : 1556
Vega : OTTHUMG00000150456
Target GenBank: BC012111

 

Reference Sequence Alignment

  

HsCD00329559      ATGGAGCTGTCTGCAGTGGGCGAGCGGGTCTTCGCGGCCGAATCCATCATCAAACGGCGG
NM_014292.3       ATGGAGCTGTCTGCAGTGGGCGAGCGGGTCTTCGCGGCCGAATCCATCATCAAACGGCGG
                  ************************************************************

HsCD00329559      ATCCGAAAGGGACGCATCGAGTACCTGGTGAAATGGAAGGGGTGGGCGATCAAGTACAGC
NM_014292.3       ATCCGAAAGGGACGCATCGAGTACCTGGTGAAATGGAAGGGGTGGGCGATCAAGTACAGC
                  ************************************************************

HsCD00329559      ACTTGGGAGCCCGAGGAGAACATCCTGGACTCGCGGCTCATTGCAGCCTTCGAACAAAAG
NM_014292.3       ACTTGGGAGCCCGAGGAGAACATCCTGGACTCGCGGCTCATTGCAGCCTTCGAACAAAAG
                  ************************************************************

HsCD00329559      GAGAGGGAGCGTGAGCTGTATGGGCCCAAGAAGAGGGGACCCAAACCCAAAACTTTCCTC
NM_014292.3       GAGAGGGAGCGTGAGCTGTATGGGCCCAAGAAGAGGGGACCCAAACCCAAAACTTTCCTC
                  ************************************************************

HsCD00329559      CTGAAGGCGCGGGCCCAGGCCGAGGCCCTCCGCATCAGTGATGTGCATTTCTCTGTCAAG
NM_014292.3       CTGAAGGCGCGGGCCCAGGCCGAGGCCCTCCGCATCAGTGATGTGCATTTCTCTGTCAAG
                  ************************************************************

HsCD00329559      CCGAGCGCCAGTGCCTCCTCGCCCAAGCTGCACTCCAGCGCAGCCGTGCACCGGCTCAAG
NM_014292.3       CCGAGCGCCAGTGCCTCCTCGCCCAAGCTGCACTCCAGCGCAGCCGTGCACCGGCTCAAG
                  ************************************************************

HsCD00329559      AAGGACATCCGCCGCTGCCACCGTATGTCCCGCCGTCCCCTGCCCCGCCCGGACCCGCAG
NM_014292.3       AAGGACATCCGCCGCTGCCACCGTATGTCCCGCCGTCCCCTGCCCCGCCCGGACCCGCAG
                  ************************************************************

HsCD00329559      GGGGGCAGCCCCGGACTGCGCCCGCCCATTTCGCCCTTCTCGGAGACGGTGCGCATCATC
NM_014292.3       GGGGGCAGCCCCGGACTGCGCCCGCCCATTTCGCCCTTCTCGGAGACGGTGCGCATCATC
                  ************************************************************

HsCD00329559      AACCGCAAGGTGAAGCCGCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTG
NM_014292.3       AACCGCAAGGTGAAGCCGCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTG
                  ************************************************************

HsCD00329559      ATCGACAAGGGCGCTGGCGGCGGGGGCGCCGGGCAGGGGGCCGGGGCGCTGGCCCGCCCC
NM_014292.3       ATCGACAAGGGCGCTGGCGGCGGGGGCGCCGGGCAGGGGGCCGGGGCGCTGGCCCGCCCC
                  ************************************************************

HsCD00329559      AAAGTCCCCTCGCGGAACCGCGTTATAGGCAAGAGCAAGAAGTTCAGCGAGAGCGTCCTG
NM_014292.3       AAAGTCCCCTCGCGGAACCGCGTTATAGGCAAGAGCAAGAAGTTCAGCGAGAGCGTCCTG
                  ************************************************************

HsCD00329559      CGTACACAGATCCGCCACATGAAGTTCGGCGCCTTTGCGCTGTACAAGCCTCCGCCCGCC
NM_014292.3       CGTACACAGATCCGCCACATGAAGTTCGGCGCCTTTGCGCTGTACAAGCCTCCGCCCGCC
                  ************************************************************

HsCD00329559      CCCCTGGTAGCCCCGTCCCCCGGCAAGGCTGAGGCCTCAGCCCCGGGCCCTGGGCTACTT
NM_014292.3       CCCCTGGTAGCCCCGTCCCCCGGCAAGGCTGAGGCCTCAGCCCCGGGCCCTGGGCTACTT
                  ************************************************************

HsCD00329559      CTGGCCGCCCCCGCCGCCCCCTACGACGCCCGCAGCTCTGGCTCCTCCGGCTGCCCCTCG
NM_014292.3       CTGGCCGCCCCCGCCGCCCCCTACGACGCCCGCAGCTCTGGCTCCTCCGGCTGCCCCTCG
                  ************************************************************

HsCD00329559      CCTACACCACAGTCCTCTGACCCCGACGACACGCTCCCCAAGCTCCTCCCCGAGACCGTG
NM_014292.3       CCTACACCACAGTCCTCTGACCCCGACGACACGCCCCCCAAGCTCCTCCCCGAGACCGTG
                  ********************************** *************************

HsCD00329559      AGCCCATCCGCCCCCAGCTGGCGCGAGCCGGAGGTGCTCGACCTGTCCCTCCCTCCCGAG
NM_014292.3       AGCCCATCCGCCCCCAGCTGGCGCGAGCCGGAGGTGCTCGACCTGTCCCTCCCTCCCGAG
                  ************************************************************

HsCD00329559      TCGGCAGCCACCAGCAAGCGGGCACCGCCTGAGGTCACAGCTGCTGCCGGCCCGGCACCT
NM_014292.3       TCGGCAGCCACCAGCAAGCGGGCACCGCCTGAGGTCACAGCTGCTGCCGGCCCGGCACCT
                  ************************************************************

HsCD00329559      CCCACGGCCCCTGAGCCCGCCGGTGCCTCCTCCGAGCCCGAGGCTGGGGACTGGCGCCCC
NM_014292.3       CCCACGGCCCCTGAGCCCGCCGGTGCCTCCTCCGAGCCCGAGGCTGGGGACTGGCGCCCC
                  ************************************************************

HsCD00329559      GAGATGTCACCCTGCTCCAATGTGGTCGTCACCGATGTCACCAGCAACCTCCTGACGGTC
NM_014292.3       GAGATGTCACCCTGCTCCAATGTGGTCGTCACCGATGTCACCAGCAACCTCCTGACGGTC
                  ************************************************************

HsCD00329559      ACAATCAAGGAATTCTGCAACCCTGAGGATTTCGAGAAGGTGGCTGCTGGGGTAGCAGGC
NM_014292.3       ACAATCAAGGAATTCTGCAACCCTGAGGATTTCGAGAAGGTGGCTGCTGGGGTAGCAGGC
                  ************************************************************

HsCD00329559      GCCGCTGGGGGCGGTGGCAGCATTGGGGCGAGCAAGTTG
NM_014292.3       GCCGCTGGGGGCGGTGGCAGCATTGGGGCGAGCAAGTGA
                  ************************************* .

Pathways

Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : Oxidative Stress Induced Senescence

Protein

SMART domain : CHROMO : Chromatin organization modifier domain
UniProt : O95503
HPRD : 13005

Clone

Cloning Information : 269057
HIP Master Clone ID : 162595
Original Clone ID : FLH269057.01L

Organism


TAX_ID : 9606
Species Specific ID: 23466

Genome

Chromosome : 22
Map Location : 22q13.1
Ensembl : ENSG00000183741

Reagents

Labome : CBX6-antibody