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FLI1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FLI1
Gene Name:  Fli-1 proto-oncogene, ETS transcription factor
Sequence             
Original Clone ID: FLH269058.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329560

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1359nts         Open reading frame : 1 to 1359
Reference Sequence Alignment

ATGGACGGGACTATTAAGGAGGCTCTGTCGGTGGTGAGCGACGACCAGTCCCTCTTTGAC
TCAGCGTACGGAGCGGCAGCCCATCTCCCCAAGGCCGACATGACTGCCTCGGGGAGTCCT
GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG
CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG
GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG
AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC
GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA
TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG
AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC
CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG
GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT
TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA
AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA
GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG
ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC
ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC
TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT
TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT
GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG
TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT
GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA
CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT
AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1482
Start on reference sequence 124
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FLI1
Symbol Nomenclature : FLI1
SYNONYM : EWSR2; SIC-1
Designation : Ewing sarcoma breakpoint region 2|Friend leukemia integration 1 transcription factor|Friend leukemia virus integration 1|proto-oncogene Fli-1|transcription factor ERGB
Full Nomenclature : Fli-1 proto-oncogene, ETS transcription factor
GENEID : 2313
HGNC : 3749
MIM : 193067
Vega : OTTHUMG00000165792
Target GenBank: BC001670

 

Reference Sequence Alignment

  

HsCD00329560        ATGGACGGGACTATTAAGGAGGCTCTGTCGGTGGTGAGCGACGACCAGTCCCTCTTTGAC
NM_002017.4         ATGGACGGGACTATTAAGGAGGCTCTGTCGGTGGTGAGCGACGACCAGTCCCTCTTTGAC
NM_001167681.2      ------------------------------------------------------------
                                                                                

HsCD00329560        TCAGCGTACGGAGCGGCAGCCCATCTCCCCAAGGCCGACATGACTGCCTCGGGGAGTCCT
NM_002017.4         TCAGCGTACGGAGCGGCAGCCCATCTCCCCAAGGCCGACATGACTGCCTCGGGGAGTCCT
NM_001167681.2      ---------------------------------------ATGACTGCCTCGGGGAGTCCT
                                                           *********************

HsCD00329560        GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG
NM_002017.4         GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG
NM_001167681.2      GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG
                    ************************************************************

HsCD00329560        CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG
NM_002017.4         CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG
NM_001167681.2      CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG
                    ************************************************************

HsCD00329560        GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG
NM_002017.4         GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG
NM_001167681.2      GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG
                    ************************************************************

HsCD00329560        AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC
NM_002017.4         AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC
NM_001167681.2      AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC
                    ************************************************************

HsCD00329560        GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA
NM_002017.4         GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA
NM_001167681.2      GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA
                    ************************************************************

HsCD00329560        TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG
NM_002017.4         TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG
NM_001167681.2      TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG
                    ************************************************************

HsCD00329560        AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC
NM_002017.4         AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC
NM_001167681.2      AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC
                    ************************************************************

HsCD00329560        CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG
NM_002017.4         CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG
NM_001167681.2      CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG
                    ************************************************************

HsCD00329560        GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT
NM_002017.4         GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT
NM_001167681.2      GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT
                    ************************************************************

HsCD00329560        TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA
NM_002017.4         TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA
NM_001167681.2      TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA
                    ************************************************************

HsCD00329560        AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA
NM_002017.4         AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA
NM_001167681.2      AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA
                    ************************************************************

HsCD00329560        GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG
NM_002017.4         GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG
NM_001167681.2      GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG
                    ************************************************************

HsCD00329560        ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC
NM_002017.4         ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC
NM_001167681.2      ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC
                    ************************************************************

HsCD00329560        ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC
NM_002017.4         ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC
NM_001167681.2      ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC
                    ************************************************************

HsCD00329560        TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT
NM_002017.4         TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT
NM_001167681.2      TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT
                    ************************************************************

HsCD00329560        TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT
NM_002017.4         TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT
NM_001167681.2      TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT
                    ************************************************************

HsCD00329560        GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG
NM_002017.4         GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG
NM_001167681.2      GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG
                    ************************************************************

HsCD00329560        TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT
NM_002017.4         TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT
NM_001167681.2      TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT
                    ************************************************************

HsCD00329560        GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA
NM_002017.4         GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA
NM_001167681.2      GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA
                    ************************************************************

HsCD00329560        CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT
NM_002017.4         CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT
NM_001167681.2      CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT
                    ************************************************************

HsCD00329560        AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTTG
NM_002017.4         AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTAG
NM_001167681.2      AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTAG
                    *************************************:*

Pathways

Panther : PDGF signaling pathway

Protein

SMART domain : ETS : erythroblast transformation specific domain
SMART domain : SAM_PNT : SAM / Pointed domain
UniProt : Q01543
UniProt : B4DTC6
HPRD : 01901

Clone

Cloning Information : 269058
HIP Master Clone ID : 162691
Original Clone ID : FLH269058.01L

Organism


TAX_ID : 9606
Species Specific ID: 2313

Genome

Chromosome : 11
Map Location : 11q24.1-q24.3
Ensembl : ENSG00000151702

Reagents

Labome : Fli-1-antibody