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CREB3L1 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CREB3L1
Gene Name:  cAMP responsive element binding protein 3-like 1
Sequence             
Original Clone ID: FLH269063.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329564

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1560nts         Open reading frame : 1 to 1560
Reference Sequence Alignment

ATGGACGCCGTCTTGGAACCCTTCCCGGCCGACAGGCTGTTCCCCGGATCCAGCTTCCTG
GACTTGGGGGATCTGAACGAGTCGGACTTCCTCAACAATGCGCACTTTCCTGAGCACCTG
GACCACTTTACGGAGAACATGGAGGACTTCTCCAATGACCTGTTCAGCAGCTTCTTTGAT
GACCCTGTGCTGGATGAGAAGAGCCCTCTATTGGACATGGAACTGGACTCCCCTACGCCA
GGCATCCAGGCGGAGCACAGCTACTCCCTGAGCGGCGACTCAGCGCCCCAGAGCCCCCTT
GTGCCCATCAAGATGGAGGACACCACCCAAGATGCAGAGCATGGAGCATGGGCGCTGGGA
CACAAACTGTGCTCCATCATGGTGAAGCAGGAGCAGAGCCCGGAGCTGCCCGTGGACCCT
CTGGCTGCCCCCTCGGCCATGGCTGCCGCGGCCGCCATGGCCACCACCCCGCTGCTGGGC
CTCAGCCCCTTGTCCAGGCTGCCCATCCCCCACCAGGCCCCGGGAGAGATGACTCAGCTG
CCAGTGATCAAAGCAGAGCCTCTGGAGGTGAACCAGTTCCTCAAAGTGACACCGGAGGAC
CTGGTGCAGATGCCTCCGACGCCCCCCAGCAGCCATGGCAGTGACAGCGACGGCTCCCAG
AGTCCCCGCTCTCTGCCCCCCTCCAGCCCTGTCAGGCCCATGGCGCGCTCCTCCACGGCC
ATCTCCACCTCCCCACTCCTCACTCCCCCTCACAAATTACAGGGGACATCAGGGCCACTG
CTCCTGACAGAGGAGGAGAAGCGGACCCTGATTGCTGAGGGCTACCCCATCCCCACAAAA
CTCCCCCTCACCAAAGCCGAGGAGAAGGCCTTGAAGAGAGTCCGGAGGAAAATCAAGAAC
AAGATCTCAGCCCAGGAGAGCCGTCGTAAGAAGAAGGAGTATGTGGAGTGTCTAGAAAAG
AAGGTGGAGACATTTACATCTGAGAACAATGAACTGTGGAAGAAGGTGGAGACCCTGGAG
AATGCCAACAGGACCCTGCTCCAGCAGCTGCAGAAACTCCAGACTCTGGTCACCAACAAG
ATCTCCAGACCTTACAAGATGGCCGCCACCCAGACTGGGACCTGCCTCATGGTGGCAGCC
TTGTGCTTTGTTCTGGTGCTGGGCTCCCTCGTGCCCTGCCTTCCCGAGTTCTCCTCCGGC
TCCCAGACTGTGAAGGAAGACCCCCTGGCCGCAGACGGCGTCTACACGGCCAGCCAGATG
CCCTCCCGAAGCCTCCTATTCTACGATGACGGGGCAGGCTTATGGGAAGATGGCCGCAGC
ACCCTGCTGCCCATGGAGCCCCCAGATGGCTGGGAAATCAACCCCGGGGGGCCGGCAGAG
CAGCGGCCCCGGGACCACCTGCAGCATGATCACCTGGACAGCACCCACGAGACCACCAAG
TACCTGAGTGAGGCCTGGCCTAAAGACGGTGGAAACGGCACCAGCCCCGACTTCTCCCAC
TCCAAGGAGTGGTTCCACGACAGGGATCTGGGCCCCAACACCACCATCAAACTCTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1677
Start on reference sequence 118
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CREB3L1
Symbol Nomenclature : CREB3L1
SYNONYM : OASIS
Designation : BBF-2 homolog|cAMP-responsive element-binding protein 3-like protein 1|cyclic AMP-responsive element-binding protein 3-like protein 1|old astrocyte specifically-induced substance
Full Nomenclature : cAMP responsive element binding protein 3-like 1
GENEID : 90993
Locus Tag : PSEC0238
HGNC : 18856
Vega : OTTHUMG00000166423
Target GenBank: BC014097

 

Reference Sequence Alignment

  

HsCD00329564      ATGGACGCCGTCTTGGAACCCTTCCCGGCCGACAGGCTGTTCCCCGGATCCAGCTTCCTG
NM_052854.3       ATGGACGCCGTCTTGGAACCCTTCCCGGCCGACAGGCTGTTCCCCGGATCCAGCTTCCTG
                  ************************************************************

HsCD00329564      GACTTGGGGGATCTGAACGAGTCGGACTTCCTCAACAATGCGCACTTTCCTGAGCACCTG
NM_052854.3       GACTTGGGGGATCTGAACGAGTCGGACTTCCTCAACAATGCGCACTTTCCTGAGCACCTG
                  ************************************************************

HsCD00329564      GACCACTTTACGGAGAACATGGAGGACTTCTCCAATGACCTGTTCAGCAGCTTCTTTGAT
NM_052854.3       GACCACTTTACGGAGAACATGGAGGACTTCTCCAATGACCTGTTCAGCAGCTTCTTTGAT
                  ************************************************************

HsCD00329564      GACCCTGTGCTGGATGAGAAGAGCCCTCTATTGGACATGGAACTGGACTCCCCTACGCCA
NM_052854.3       GACCCTGTGCTGGATGAGAAGAGCCCTCTATTGGACATGGAACTGGACTCCCCTACGCCA
                  ************************************************************

HsCD00329564      GGCATCCAGGCGGAGCACAGCTACTCCCTGAGCGGCGACTCAGCGCCCCAGAGCCCCCTT
NM_052854.3       GGCATCCAGGCGGAGCACAGCTACTCCCTGAGCGGCGACTCAGCGCCCCAGAGCCCCCTT
                  ************************************************************

HsCD00329564      GTGCCCATCAAGATGGAGGACACCACCCAAGATGCAGAGCATGGAGCATGGGCGCTGGGA
NM_052854.3       GTGCCCATCAAGATGGAGGACACCACCCAAGATGCAGAGCATGGAGCATGGGCGCTGGGA
                  ************************************************************

HsCD00329564      CACAAACTGTGCTCCATCATGGTGAAGCAGGAGCAGAGCCCGGAGCTGCCCGTGGACCCT
NM_052854.3       CACAAACTGTGCTCCATCATGGTGAAGCAGGAGCAGAGCCCGGAGCTGCCCGTGGACCCT
                  ************************************************************

HsCD00329564      CTGGCTGCCCCCTCGGCCATGGCTGCCGCGGCCGCCATGGCCACCACCCCGCTGCTGGGC
NM_052854.3       CTGGCTGCCCCCTCGGCCATGGCTGCCGCGGCCGCCATGGCCACCACCCCGCTGCTGGGC
                  ************************************************************

HsCD00329564      CTCAGCCCCTTGTCCAGGCTGCCCATCCCCCACCAGGCCCCGGGAGAGATGACTCAGCTG
NM_052854.3       CTCAGCCCCTTGTCCAGGCTGCCCATCCCCCACCAGGCCCCGGGAGAGATGACTCAGCTG
                  ************************************************************

HsCD00329564      CCAGTGATCAAAGCAGAGCCTCTGGAGGTGAACCAGTTCCTCAAAGTGACACCGGAGGAC
NM_052854.3       CCAGTGATCAAAGCAGAGCCTCTGGAGGTGAACCAGTTCCTCAAAGTGACACCGGAGGAC
                  ************************************************************

HsCD00329564      CTGGTGCAGATGCCTCCGACGCCCCCCAGCAGCCATGGCAGTGACAGCGACGGCTCCCAG
NM_052854.3       CTGGTGCAGATGCCTCCGACGCCCCCCAGCAGCCATGGCAGTGACAGCGACGGCTCCCAG
                  ************************************************************

HsCD00329564      AGTCCCCGCTCTCTGCCCCCCTCCAGCCCTGTCAGGCCCATGGCGCGCTCCTCCACGGCC
NM_052854.3       AGTCCCCGCTCTCTGCCCCCCTCCAGCCCTGTCAGGCCCATGGCGCGCTCCTCCACGGCC
                  ************************************************************

HsCD00329564      ATCTCCACCTCCCCACTCCTCACTCCCCCTCACAAATTACAGGGGACATCAGGGCCACTG
NM_052854.3       ATCTCCACCTCCCCACTCCTCACTGCCCCTCACAAATTACAGGGGACATCAGGGCCACTG
                  ************************ ***********************************

HsCD00329564      CTCCTGACAGAGGAGGAGAAGCGGACCCTGATTGCTGAGGGCTACCCCATCCCCACAAAA
NM_052854.3       CTCCTGACAGAGGAGGAGAAGCGGACCCTGATTGCTGAGGGCTACCCCATCCCCACAAAA
                  ************************************************************

HsCD00329564      CTCCCCCTCACCAAAGCCGAGGAGAAGGCCTTGAAGAGAGTCCGGAGGAAAATCAAGAAC
NM_052854.3       CTCCCCCTCACCAAAGCCGAGGAGAAGGCCTTGAAGAGAGTCCGGAGGAAAATCAAGAAC
                  ************************************************************

HsCD00329564      AAGATCTCAGCCCAGGAGAGCCGTCGTAAGAAGAAGGAGTATGTGGAGTGTCTAGAAAAG
NM_052854.3       AAGATCTCAGCCCAGGAGAGCCGTCGTAAGAAGAAGGAGTATGTGGAGTGTCTAGAAAAG
                  ************************************************************

HsCD00329564      AAGGTGGAGACATTTACATCTGAGAACAATGAACTGTGGAAGAAGGTGGAGACCCTGGAG
NM_052854.3       AAGGTGGAGACATTTACATCTGAGAACAATGAACTGTGGAAGAAGGTGGAGACCCTGGAG
                  ************************************************************

HsCD00329564      AATGCCAACAGGACCCTGCTCCAGCAGCTGCAGAAACTCCAGACTCTGGTCACCAACAAG
NM_052854.3       AATGCCAACAGGACCCTGCTCCAGCAGCTGCAGAAACTCCAGACTCTGGTCACCAACAAG
                  ************************************************************

HsCD00329564      ATCTCCAGACCTTACAAGATGGCCGCCACCCAGACTGGGACCTGCCTCATGGTGGCAGCC
NM_052854.3       ATCTCCAGACCTTACAAGATGGCCGCCACCCAGACTGGGACCTGCCTCATGGTGGCAGCC
                  ************************************************************

HsCD00329564      TTGTGCTTTGTTCTGGTGCTGGGCTCCCTCGTGCCCTGCCTTCCCGAGTTCTCCTCCGGC
NM_052854.3       TTGTGCTTTGTTCTGGTGCTGGGCTCCCTCGTGCCCTGCCTTCCCGAGTTCTCCTCCGGC
                  ************************************************************

HsCD00329564      TCCCAGACTGTGAAGGAAGACCCCCTGGCCGCAGACGGCGTCTACACGGCCAGCCAGATG
NM_052854.3       TCCCAGACTGTGAAGGAAGACCCCCTGGCCGCAGACGGCGTCTACACGGCCAGCCAGATG
                  ************************************************************

HsCD00329564      CCCTCCCGAAGCCTCCTATTCTACGATGACGGGGCAGGCTTATGGGAAGATGGCCGCAGC
NM_052854.3       CCCTCCCGAAGCCTCCTATTCTACGATGACGGGGCAGGCTTATGGGAAGATGGCCGCAGC
                  ************************************************************

HsCD00329564      ACCCTGCTGCCCATGGAGCCCCCAGATGGCTGGGAAATCAACCCCGGGGGGCCGGCAGAG
NM_052854.3       ACCCTGCTGCCCATGGAGCCCCCAGATGGCTGGGAAATCAACCCCGGGGGGCCGGCAGAG
                  ************************************************************

HsCD00329564      CAGCGGCCCCGGGACCACCTGCAGCATGATCACCTGGACAGCACCCACGAGACCACCAAG
NM_052854.3       CAGCGGCCCCGGGACCACCTGCAGCATGATCACCTGGACAGCACCCACGAGACCACCAAG
                  ************************************************************

HsCD00329564      TACCTGAGTGAGGCCTGGCCTAAAGACGGTGGAAACGGCACCAGCCCCGACTTCTCCCAC
NM_052854.3       TACCTGAGTGAGGCCTGGCCTAAAGACGGTGGAAACGGCACCAGCCCCGACTTCTCCCAC
                  ************************************************************

HsCD00329564      TCCAAGGAGTGGTTCCACGACAGGGATCTGGGCCCCAACACCACCATCAAACTCTCCTTG
NM_052854.3       TCCAAGGAGTGGTTCCACGACAGGGATCTGGGCCCCAACACCACCATCAAACTCTCCTAG
                  **********************************************************:*

Pathways

Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Transcription regulation by bZIP transcription factor
WikiPathway : G1 to S cell cycle control

Protein

SMART domain : BRLZ : basic region leucin zipper
UniProt : Q96BA8
HPRD : 16751

Clone

Cloning Information : 269063
HIP Master Clone ID : 162802
Original Clone ID : FLH269063.01L

Organism


TAX_ID : 9606
Species Specific ID: 90993

Genome

Chromosome : 11
Map Location : 11p11.2
Ensembl : ENSG00000157613

Reagents

Labome : OASIS-antibody