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TGIF2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TGIF2
Gene Name:  TGFB-induced factor homeobox 2
Sequence             
Original Clone ID: FLH269068.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329569

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 714nts         Open reading frame : 1 to 714
Reference Sequence Alignment

ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 874
Start on reference sequence 161
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TGIF2
Symbol Nomenclature : TGIF2
Designation : 5'-TG-3' interacting factor 2|TGF(beta)-induced transcription factor 2|TGF-beta-induced transcription factor 2|TGFB-induced factor 2 (TALE family homeobox)|homeobox protein TGIF2|transcription growth factor-beta-induced factor 2
Full Nomenclature : TGFB-induced factor homeobox 2
GENEID : 60436
HGNC : 15764
MIM : 607294
Vega : OTTHUMG00000032382
Vega : OTTHUMG00000172332
Target GenBank: BC012816

 

Reference Sequence Alignment

  

HsCD00329569        ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
NM_001199515.1      ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
NM_001199514.1      ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
NM_021809.6         ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
NM_001199513.1      ATGTCGGACAGTGATCTAGGTGAGGACGAAGGCCTCCTCTCCCTGGCGGGCAAAAGGAAG
                    ************************************************************

HsCD00329569        CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
NM_001199515.1      CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
NM_001199514.1      CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
NM_021809.6         CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
NM_001199513.1      CGCAGGGGGAACCTGCCCAAGGAGTCGGTGAAGATCCTCCGGGACTGGCTGTACTTGCAC
                    ************************************************************

HsCD00329569        CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
NM_001199515.1      CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
NM_001199514.1      CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
NM_021809.6         CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
NM_001199513.1      CGCTACAACGCCTACCCCTCAGAGCAGGAGAAGCTGAGCCTTTCTGGACAGACCAACCTG
                    ************************************************************

HsCD00329569        TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
NM_001199515.1      TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
NM_001199514.1      TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
NM_021809.6         TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
NM_001199513.1      TCAGTGCTGCAAATATGTAACTGGTTCATCAATGCCCGGCGGCGGCTTCTCCCAGACATG
                    ************************************************************

HsCD00329569        CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
NM_001199515.1      CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
NM_001199514.1      CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
NM_021809.6         CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
NM_001199513.1      CTTCGGAAGGATGGCAAAGACCCTAATCAGTTTACCATTTCCCGCCGCGGGGGTAAGGCC
                    ************************************************************

HsCD00329569        TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
NM_001199515.1      TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
NM_001199514.1      TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
NM_021809.6         TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
NM_001199513.1      TCAGATGTGGCCCTCCCCCGTGGCAGCAGCCCCTCAGTGCTGGCTGTGTCTGTCCCAGCC
                    ************************************************************

HsCD00329569        CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
NM_001199515.1      CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
NM_001199514.1      CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
NM_021809.6         CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
NM_001199513.1      CCCACCAATGTGCTCTCCCTGTCTGTGTGCTCCATGCCGCTTCACTCAGGCCAGGGGGAA
                    ************************************************************

HsCD00329569        AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
NM_001199515.1      AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
NM_001199514.1      AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
NM_021809.6         AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
NM_001199513.1      AAGCCAGCAGCCCCTTTCCCACGTGGGGAGCTGGAGTCTCCCAAGCCCCTGGTGACCCCT
                    ************************************************************

HsCD00329569        GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
NM_001199515.1      GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
NM_001199514.1      GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
NM_021809.6         GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
NM_001199513.1      GGTAGCACACTTACTCTGCTGACCAGGGCTGAGGCTGGAAGCCCCACAGGTGGACTCTTC
                    ************************************************************

HsCD00329569        AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
NM_001199515.1      AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
NM_001199514.1      AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
NM_021809.6         AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
NM_001199513.1      AACACGCCACCACCCACACCCCCAGAGCAGGACAAAGAGGACTTCAGCAGCTTCCAGCTG
                    ************************************************************

HsCD00329569        CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
NM_001199515.1      CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
NM_001199514.1      CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
NM_021809.6         CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
NM_001199513.1      CTGGTGGAGGTGGCGCTACAGAGGGCTGCTGAGATGGAGCTTCAGAAGCAGCAGGACCCA
                    ************************************************************

HsCD00329569        TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTTG
NM_001199515.1      TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTAG
NM_001199514.1      TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTAG
NM_021809.6         TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTAG
NM_001199513.1      TCACTCCCATTACTGCACACTCCCATCCCTTTAGTCTCTGAAAATCCCCAGTAG
                    ****************************************************:*

Pathways

NCI : Regulation of nuclear SMAD2/3 signaling
Reactome : Disease
Reactome : Downregulation of SMAD2/3:SMAD4 transcriptional activity
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
Reactome : Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer

Protein

SMART domain : HOX : Homeodomain
UniProt : Q9GZN2
HPRD : 06293

Clone

Cloning Information : 269068
HIP Master Clone ID : 174093
Original Clone ID : FLH269068.01L

Organism


TAX_ID : 9606
Species Specific ID: 60436

Genome

Chromosome : 20
Map Location : 20q11.23
Ensembl : ENSG00000118707

Reagents

Labome : TGIF2-antibody