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ANP32A (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ANP32A
Gene Name:  acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Sequence             
Original Clone ID: FLH269069.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329570

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 750nts         Open reading frame : 1 to 750
Reference Sequence Alignment

ATGGAGATGGGCAGACGGATTCATTTAGAGCTGCGGAACAGGACGCCCTCTGATGTGAAA
GAACTTGTCCTGGACAACAGTCGGTCGAATGAAGGCAAACTCGAAGGCCTCACAGATGAA
TTTGAAGAACTGGAATTCTTAAGTACAATCAACGTAGGCCTCACCTCAATCGCAAACTTA
CCAAAGTTAAACAAACTTAAGAAGCTTGAACTAAGCGATAACAGAGTCTCAGGGGGCCTG
GAAGTATTGGCAGAAAAGTGTCCGAACCTCACGCATCTAAATTTAAGTGGCAACAAAATT
AAAGACCTCAGCACAATAGAGCCACTGAAAAAGTTAGAAAACCTCAAGAGCTTAGACCTT
TTCAATTGCGAGGTAACCAACCTGAACGACTACCGAGAAAATGTGTTCAAGCTCCTCCCG
CAACTCACATATCTCGACGGCTATGACCGGGACGACAAGGAGGCCCCTGACTCGGATGCT
GAGGGCTACGTGGAGGGCCTGGATGATGAGGAGGAGGATGAGGATGAGGAGGAGTATGAT
GAAGATGCTCAGGTAGTGGAAGACGAGGAGGACGAGGATGAGGAGGAGGAAGGTGAAGAG
GAGGACGTGAGTGGAGAGGAGGAGGAGGATGAAGAAGGTTATAACGATGGAGAGGTAGAT
GACGAGGAAGATGAAGAAGAGCTTGGTGAAGAAGAAAGGGGTCAGAAGCGAAAACGAGAA
CCTGAAGATGAGGGAGAAGATGATGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 883
Start on reference sequence 134
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ANP32A
Symbol Nomenclature : ANP32A
SYNONYM : C15orf1; HPPCn; I1PP2A; LANP; MAPM; PHAP1; PHAPI; PP32
Designation : acidic leucine-rich nuclear phosphoprotein 32 family member A|acidic nuclear phosphoprotein pp32|cerebellar leucine rich acidic nuclear protein|hepatopoietin Cn|inhibitor-1 of protein phosphatase-2A|leucine-rich acidic nuclear protein|mapmodulin|potent heat-stable protein phosphatase 2A inhibitor I1PP2A|putative HLA-DR-associated protein I|putative human HLA class II associated protein I
Full Nomenclature : acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
GENEID : 8125
HGNC : 13233
MIM : 600832
Vega : OTTHUMG00000154502
Target GenBank: BC007200

 

Reference Sequence Alignment

  

HsCD00329570      ATGGAGATGGGCAGACGGATTCATTTAGAGCTGCGGAACAGGACGCCCTCTGATGTGAAA
NM_006305.3       ATGGAGATGGGCAGACGGATTCATTTAGAGCTGCGGAACAGGACGCCCTCTGATGTGAAA
                  ************************************************************

HsCD00329570      GAACTTGTCCTGGACAACAGTCGGTCGAATGAAGGCAAACTCGAAGGCCTCACAGATGAA
NM_006305.3       GAACTTGTCCTGGACAACAGTCGGTCGAATGAAGGCAAACTCGAAGGCCTCACAGATGAA
                  ************************************************************

HsCD00329570      TTTGAAGAACTGGAATTCTTAAGTACAATCAACGTAGGCCTCACCTCAATCGCAAACTTA
NM_006305.3       TTTGAAGAACTGGAATTCTTAAGTACAATCAACGTAGGCCTCACCTCAATCGCAAACTTA
                  ************************************************************

HsCD00329570      CCAAAGTTAAACAAACTTAAGAAGCTTGAACTAAGCGATAACAGAGTCTCAGGGGGCCTG
NM_006305.3       CCAAAGTTAAACAAACTTAAGAAGCTTGAACTAAGCGATAACAGAGTCTCAGGGGGCCTG
                  ************************************************************

HsCD00329570      GAAGTATTGGCAGAAAAGTGTCCGAACCTCACGCATCTAAATTTAAGTGGCAACAAAATT
NM_006305.3       GAAGTATTGGCAGAAAAGTGTCCGAACCTCACGCATCTAAATTTAAGTGGCAACAAAATT
                  ************************************************************

HsCD00329570      AAAGACCTCAGCACAATAGAGCCACTGAAAAAGTTAGAAAACCTCAAGAGCTTAGACCTT
NM_006305.3       AAAGACCTCAGCACAATAGAGCCACTGAAAAAGTTAGAAAACCTCAAGAGCTTAGACCTT
                  ************************************************************

HsCD00329570      TTCAATTGCGAGGTAACCAACCTGAACGACTACCGAGAAAATGTGTTCAAGCTCCTCCCG
NM_006305.3       TTCAATTGCGAGGTAACCAACCTGAACGACTACCGAGAAAATGTGTTCAAGCTCCTCCCG
                  ************************************************************

HsCD00329570      CAACTCACATATCTCGACGGCTATGACCGGGACGACAAGGAGGCCCCTGACTCGGATGCT
NM_006305.3       CAACTCACATATCTCGACGGCTATGACCGGGACGACAAGGAGGCCCCTGACTCGGATGCT
                  ************************************************************

HsCD00329570      GAGGGCTACGTGGAGGGCCTGGATGATGAGGAGGAGGATGAGGATGAGGAGGAGTATGAT
NM_006305.3       GAGGGCTACGTGGAGGGCCTGGATGATGAGGAGGAGGATGAGGATGAGGAGGAGTATGAT
                  ************************************************************

HsCD00329570      GAAGATGCTCAGGTAGTGGAAGACGAGGAGGACGAGGATGAGGAGGAGGAAGGTGAAGAG
NM_006305.3       GAAGATGCTCAGGTAGTGGAAGACGAGGAGGACGAGGATGAGGAGGAGGAAGGTGAAGAG
                  ************************************************************

HsCD00329570      GAGGACGTGAGTGGAGAGGAGGAGGAGGATGAAGAAGGTTATAACGATGGAGAGGTAGAT
NM_006305.3       GAGGACGTGAGTGGAGAGGAGGAGGAGGATGAAGAAGGTTATAACGATGGAGAGGTAGAT
                  ************************************************************

HsCD00329570      GACGAGGAAGATGAAGAAGAGCTTGGTGAAGAAGAAAGGGGTCAGAAGCGAAAACGAGAA
NM_006305.3       GACGAGGAAGATGAAGAAGAGCTTGGTGAAGAAGAAAGGGGTCAGAAGCGAAAACGAGAA
                  ************************************************************

HsCD00329570      CCTGAAGATGAGGGAGAAGATGATGACTTG
NM_006305.3       CCTGAAGATGAGGGAGAAGATGATGACTAA
                  ****************************:.

Pathways

NCI : Validated nuclear estrogen receptor alpha network
Reactome : Gene Expression
Reactome : HuR stabilizes mRNA
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements

Protein

SMART domain : LRRcap : occurring C-terminal to leucine-rich repeats
UniProt : P39687
HPRD : 09014

Clone

Cloning Information : 269069
HIP Master Clone ID : 174105
Original Clone ID : FLH269069.01L

Organism


TAX_ID : 9606
Species Specific ID: 8125

Genome

Chromosome : 15
Map Location : 15q23
Ensembl : ENSG00000140350

Reagents

Labome : ANP32A-antibody