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NFE2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NFE2
Gene Name:  nuclear factor, erythroid 2
Sequence             
Original Clone ID: FLH269078.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329576

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1122nts         Open reading frame : 1 to 1122
Reference Sequence Alignment

ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1385
Start on reference sequence 264
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NFE2
Symbol Nomenclature : NFE2
SYNONYM : NF-E2; p45
Designation : leucine zipper protein NF-E2|nuclear factor (erythroid-derived 2), 45kD|nuclear factor (erythroid-derived 2), 45kDa|nuclear factor, erythroid-derived 2 45 kDa subunit|p45 NF-E2|transcription factor NF-E2 45 kDa subunit
Full Nomenclature : nuclear factor, erythroid 2
GENEID : 4778
HGNC : 7780
MIM : 601490
Vega : OTTHUMG00000169761
Target GenBank: BC005044

 

Reference Sequence Alignment

  

HsCD00329576        ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
NM_001261461.1      ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
XM_005268907.1      ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
NM_006163.2         ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
NM_001136023.2      ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAG
                    ************************************************************

HsCD00329576        CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
NM_001261461.1      CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
XM_005268907.1      CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
NM_006163.2         CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
NM_001136023.2      CTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTG
                    ************************************************************

HsCD00329576        AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
NM_001261461.1      AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
XM_005268907.1      AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
NM_006163.2         AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
NM_001136023.2      AATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCA
                    ************************************************************

HsCD00329576        CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
NM_001261461.1      CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
XM_005268907.1      CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
NM_006163.2         CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
NM_001136023.2      CCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGGCTTCCCACTTCCTCCA
                    ************************************************************

HsCD00329576        CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
NM_001261461.1      CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
XM_005268907.1      CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
NM_006163.2         CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
NM_001136023.2      CCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCCCCCATACTCCTATGGC
                    ************************************************************

HsCD00329576        AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
NM_001261461.1      AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
XM_005268907.1      AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
NM_006163.2         AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
NM_001136023.2      AACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAGTGAGCCGCTC
                    ************************************************************

HsCD00329576        CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
NM_001261461.1      CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
XM_005268907.1      CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
NM_006163.2         CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
NM_001136023.2      CAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
                    ************************************************************

HsCD00329576        GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
NM_001261461.1      GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
XM_005268907.1      GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
NM_006163.2         GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
NM_001136023.2      GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTG
                    ************************************************************

HsCD00329576        GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
NM_001261461.1      GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
XM_005268907.1      GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
NM_006163.2         GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
NM_001136023.2      GAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTAC
                    ************************************************************

HsCD00329576        CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
NM_001261461.1      CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
XM_005268907.1      CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
NM_006163.2         CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
NM_001136023.2      CCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAG
                    ************************************************************

HsCD00329576        ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
NM_001261461.1      ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
XM_005268907.1      ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
NM_006163.2         ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
NM_001136023.2      ACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGCGGGCTAAGCCCACTGCACGGGGG
                    ************************************************************

HsCD00329576        GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
NM_001261461.1      GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
XM_005268907.1      GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
NM_006163.2         GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
NM_001136023.2      GAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGAAGATTCCTTTTCCTACGGAC
                    ************************************************************

HsCD00329576        AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
NM_001261461.1      AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
XM_005268907.1      AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
NM_006163.2         AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
NM_001136023.2      AAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTACCCGCTGACA
                    ************************************************************

HsCD00329576        GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
NM_001261461.1      GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
XM_005268907.1      GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
NM_006163.2         GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
NM_001136023.2      GAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
                    ************************************************************

HsCD00329576        CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
NM_001261461.1      CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
XM_005268907.1      CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
NM_006163.2         CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
NM_001136023.2      CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGG
                    ************************************************************

HsCD00329576        CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
NM_001261461.1      CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
XM_005268907.1      CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
NM_006163.2         CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
NM_001136023.2      CTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAG
                    ************************************************************

HsCD00329576        GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
NM_001261461.1      GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
XM_005268907.1      GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
NM_006163.2         GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
NM_001136023.2      GTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAA
                    ************************************************************

HsCD00329576        TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
NM_001261461.1      TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
XM_005268907.1      TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
NM_006163.2         TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
NM_001136023.2      TCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGCAACAGGCTGCCGATGGGACCATC
                    ************************************************************

HsCD00329576        TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTTG
NM_001261461.1      TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTGA
XM_005268907.1      TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTGA
NM_006163.2         TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTGA
NM_001136023.2      TTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGACTGA
                    **************************************** .

Pathways

Reactome : Factors involved in megakaryocyte development and platelet production
Reactome : Hemostasis

Protein

SMART domain : BRLZ : basic region leucin zipper
UniProt : Q16621
UniProt : A8K3E0
HPRD : 09030

Clone

Cloning Information : 269078
HIP Master Clone ID : 174145
Original Clone ID : FLH269078.01L

Organism


TAX_ID : 9606
Species Specific ID: 4778

Genome

Chromosome : 12
Map Location : 12q13
Ensembl : ENSG00000123405

Reagents

Labome : NF-E2-antibody