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NFKBIB (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NFKBIB
Gene Name:  nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Sequence             
Original Clone ID: FLH269082.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329580

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1071nts         Open reading frame : 1 to 1071
Reference Sequence Alignment

ATGGCTGGGGTCGCGTGCTTGGGAAAAGCTGCCGACGCAGATGAATGGTGCGACAGCGGC
CTGGGCTCCCTGGGTCCGGACGCAGCGGCCCCCGGAGGACCTGGGTTGGGCGCGGAGTTG
GGCCCGGGGCTGTCGTGGGCTCCCCTCGTCTTCGGCTACGTCACTGAGGATGGGGACACG
GCACTGCACTTGGCTGTGATTCATCAGCATGAACCCTTCCTGGATTTTCTTCTAGGCTTC
TCGGCCGGCACTGAGTACATGGACCTGCAGAATGACCTAGGCCAGACAGCCCTGCACCTG
GCAGCCATCCTGGGGGAGACATCCACGGTGGAGAAGCTGTACGCAGCAGGCGCCGGGCTG
TGTGTGGCGGAGCGTAGGGGCCACACGGCGCTGCACCTGGCCTGCCGTGTGGGGGCACAC
GCCTGTGCCCGTGCCCTGCTTCAGCCCCGCCCCCGGCGCCCCAGGGAAGCCCCCGACACC
TACCTCGCTCAGGGCCCTGACCGTACTCCCGACACCAACCATACCCCTGTCGCCTTGTAC
CCCGATTCCGACTTGGAGAAGGAAGAAGAGGAGAGTGAGGAGGACTGGAAGCTGCAGCTG
GAGGCTGAAAACTACGAGGGCCACACCCCACTCCACGTGGCCGTTATCCACAAAGATGTG
GAGATGGTCCGGCTGCTCCGAGATGCTGGAGCTGACCTTGACAAACCGGAGCCCACGTGC
GGCCGGAGCCCCCTTCATTTGGCAGTGGAGGCCCAGGCAGCCGATGTGCTGGAGCTTCTC
CTGAGGGCAGGCGCGAACCCTGCTGCCCGCATGTACGGTGGCCGCACCCCACTCGGCAGT
GCCATGCTCCGGCCCAACCCCATCCTCGCCCGCCTCCTCCGTGCACACGGAGCCCCTGAG
CCCGAGGGCGAGGACGAGAAATCCGGCCCCTGCAGCAGCAGTAGCGACAGCGACAGCGGA
GACGAGGGCGATGAATACGACGACATTGTGGTTCACAGCAGCCGCAGCCAAACCCGGCTG
CCTCCCACCCCAGCCTCAAAACCTCTTCCTGACGACCCCCGCCCCGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1105
Start on reference sequence 35
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NFKBIB
Symbol Nomenclature : NFKBIB
SYNONYM : IKBB; TRIP9
Designation : I-kappa-B-beta|NF-kappa-B inhibitor beta|NF-kappa-BIB|TR-interacting protein 9|TRIP-9|ikB-B|ikB-beta|ikappaBbeta|thyroid receptor-interacting protein 9
Full Nomenclature : nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
GENEID : 4793
HGNC : 7798
MIM : 604495
Vega : OTTHUMG00000177631
Target GenBank: BC015528

 

Reference Sequence Alignment

  

HsCD00329580      ATGGCTGGGGTCGCGTGCTTGGGAAAAGCTGCCGACGCAGATGAATGGTGCGACAGCGGC
NM_002503.4       ATGGCTGGGGTCGCGTGCTTGGGAAAAGCTGCCGACGCAGATGAATGGTGCGACAGCGGC
                  ************************************************************

HsCD00329580      CTGGGCTCCCTGGGTCCGGACGCAGCGGCCCCCGGAGGACCTGGGTTGGGCGCGGAGTTG
NM_002503.4       CTGGGCTCCCTGGGTCCGGACGCAGCGGCCCCCGGAGGACCTGGGTTGGGCGCGGAGTTG
                  ************************************************************

HsCD00329580      GGCCCGGGGCTGTCGTGGGCTCCCCTCGTCTTCGGCTACGTCACTGAGGATGGGGACACG
NM_002503.4       GGCCCGGGGCTGTCGTGGGCTCCCCTCGTCTTCGGCTACGTCACTGAGGATGGGGACACG
                  ************************************************************

HsCD00329580      GCACTGCACTTGGCTGTGATTCATCAGCATGAACCCTTCCTGGATTTTCTTCTAGGCTTC
NM_002503.4       GCACTGCACTTGGCTGTGATTCATCAGCATGAACCCTTCCTGGATTTTCTTCTAGGCTTC
                  ************************************************************

HsCD00329580      TCGGCCGGCACTGAGTACATGGACCTGCAGAATGACCTAGGCCAGACAGCCCTGCACCTG
NM_002503.4       TCGGCCGGCACTGAGTACATGGACCTGCAGAATGACCTAGGCCAGACAGCCCTGCACCTG
                  ************************************************************

HsCD00329580      GCAGCCATCCTGGGGGAGACATCCACGGTGGAGAAGCTGTACGCAGCAGGCGCCGGGCTG
NM_002503.4       GCAGCCATCCTGGGGGAGACATCCACGGTGGAGAAGCTGTACGCAGCAGGCGCCGGGCTG
                  ************************************************************

HsCD00329580      TGTGTGGCGGAGCGTAGGGGCCACACGGCGCTGCACCTGGCCTGCCGTGTGGGGGCACAC
NM_002503.4       TGTGTGGCGGAGCGTAGGGGCCACACGGCGCTGCACCTGGCCTGCCGTGTGGGGGCACAC
                  ************************************************************

HsCD00329580      GCCTGTGCCCGTGCCCTGCTTCAGCCCCGCCCCCGGCGCCCCAGGGAAGCCCCCGACACC
NM_002503.4       GCCTGTGCCCGTGCCCTGCTTCAGCCCCGCCCCCGGCGCCCCAGGGAAGCCCCCGACACC
                  ************************************************************

HsCD00329580      TACCTCGCTCAGGGCCCTGACCGTACTCCCGACACCAACCATACCCCTGTCGCCTTGTAC
NM_002503.4       TACCTCGCTCAGGGCCCTGACCGTACTCCCGACACCAACCATACCCCTGTCGCCTTGTAC
                  ************************************************************

HsCD00329580      CCCGATTCCGACTTGGAGAAGGAAGAAGAGGAGAGTGAGGAGGACTGGAAGCTGCAGCTG
NM_002503.4       CCCGATTCCGACTTGGAGAAGGAAGAAGAGGAGAGTGAGGAGGACTGGAAGCTGCAGCTG
                  ************************************************************

HsCD00329580      GAGGCTGAAAACTACGAGGGCCACACCCCACTCCACGTGGCCGTTATCCACAAAGATGTG
NM_002503.4       GAGGCTGAAAACTACGAGGGCCACACCCCACTCCACGTGGCCGTTATCCACAAAGATGTG
                  ************************************************************

HsCD00329580      GAGATGGTCCGGCTGCTCCGAGATGCTGGAGCTGACCTTGACAAACCGGAGCCCACGTGC
NM_002503.4       GAGATGGTCCGGCTGCTCCGAGATGCTGGAGCTGACCTTGACAAACCGGAGCCCACGTGC
                  ************************************************************

HsCD00329580      GGCCGGAGCCCCCTTCATTTGGCAGTGGAGGCCCAGGCAGCCGATGTGCTGGAGCTTCTC
NM_002503.4       GGCCGGAGCCCCCTTCATTTGGCAGTGGAGGCCCAGGCAGCCGATGTGCTGGAGCTTCTC
                  ************************************************************

HsCD00329580      CTGAGGGCAGGCGCGAACCCTGCTGCCCGCATGTACGGTGGCCGCACCCCACTCGGCAGT
NM_002503.4       CTGAGGGCAGGCGCGAACCCTGCTGCCCGCATGTACGGTGGCCGCACCCCACTCGGCAGT
                  ************************************************************

HsCD00329580      GCCATGCTCCGGCCCAACCCCATCCTCGCCCGCCTCCTCCGTGCACACGGAGCCCCTGAG
NM_002503.4       GCCATGCTCCGGCCCAACCCCATCCTCGCCCGCCTCCTCCGTGCACACGGAGCCCCTGAG
                  ************************************************************

HsCD00329580      CCCGAGGGCGAGGACGAGAAATCCGGCCCCTGCAGCAGCAGTAGCGACAGCGACAGCGGA
NM_002503.4       CCCGAGGGCGAGGACGAGAAATCCGGCCCCTGCAGCAGCAGTAGCGACAGCGACAGCGGA
                  ************************************************************

HsCD00329580      GACGAGGGCGATGAATACGACGACATTGTGGTTCACAGCAGCCGCAGCCAAACCCGGCTG
NM_002503.4       GACGAGGGCGATGAATACGACGACATTGTGGTTCACAGCAGCCGCAGCCAAACCCGGCTG
                  ************************************************************

HsCD00329580      CCTCCCACCCCAGCCTCAAAACCTCTTCCTGACGACCCCCGCCCCGTGTTG
NM_002503.4       CCTCCCACCCCAGCCTCAAAACCTCTTCCTGACGACCCCCGCCCCGTGTGA
                  ************************************************* .

Pathways

NCI : BCR signaling pathway
Reactome : Activated TLR4 signalling
Reactome : Activation of NF-kappaB in B cells
Reactome : Adaptive Immune System
Reactome : Cytosolic sensors of pathogen-associated DNA
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : Immune System
Reactome : Innate Immune System
Reactome : MyD88 cascade initiated on plasma membrane
Reactome : MyD88 dependent cascade initiated on endosome
Reactome : MyD88-independent cascade
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
Reactome : RIP-mediated NFkB activation via ZBP1
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : TAK1 activates NFkB by phosphorylation and activation of IKKs complex
Reactome : TRAF6 Mediated Induction of proinflammatory cytokines
Reactome : TRAF6 mediated NF-kB activation
Reactome : TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Toll Like Receptor 10 (TLR10) Cascade
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor 5 (TLR5) Cascade
Reactome : Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome : Toll Like Receptor 9 (TLR9) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
Reactome : ZBP1(DAI) mediated induction of type I IFNs
WikiPathway : Apoptosis
WikiPathway : IL-1 signaling pathway
WikiPathway : Prolactin Signaling Pathway
WikiPathway : TNF alpha Signaling Pathway
WikiPathway : TWEAK Signaling Pathway

Protein

SMART domain : ANK : ankyrin repeats
UniProt : Q96LS1
UniProt : Q15653
HPRD : 07260

Clone

Cloning Information : 269082
HIP Master Clone ID : 137750
Original Clone ID : FLH269082.01L

Organism


TAX_ID : 9606
Species Specific ID: 4793

Genome

Chromosome : 19
Map Location : 19q13.1
Ensembl : ENSG00000104825

Reagents

Labome : NFKBIB-antibody