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KLF5 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLF5
Gene Name:  Kruppel-like factor 5 (intestinal)
Sequence             
Original Clone ID: FLH269086.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329584

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1374nts         Open reading frame : 1 to 1374
Reference Sequence Alignment

ATGGCTACAAGGGTGCTGAGCATGAGCGCCCGCCTGGGACCCGTGCCCCAGCCGCCGGCG
CCGCAGGACGAGCCGGTGTTCGCGCAGCTCAAGCCGGTGCTGGGCGCCGCGAATCCGGCC
CGCGACGCGGCGCTCTTCCCCGGCGAGGAGCTGAAGCACGCGCACCACCGCCCGCAGGCG
CAGCCCGCGCCCGCGCAGGCCCCGCAGCCGGCCCAGCCGCCCGCCACCGGCCCGCGGCTG
CCTCCAGAGGACCTGGTCCAGACAAGATGTGAAATGGAGAAGTATCTGACACCTCAGCTT
CCTCCAGTTCCTATAATTCCAGAGCATAAAAAGTATAGACGAGACAGTGCCTCAGTCGTA
GACCAGTTCTTCACTGACACTGAAGGGTTACCTTACAGTATCAACATGAACGTCTTCCTC
CCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCGTGCGTAACACAC
ATCAAGACAGAACCTGTTGCCATTTTCAGCCACCAGAGTGAAACGACTGCCCCTCCTCCG
GCCCCGACCCAGGCCCTCCCTGAGTTCACCAGTATATTCAGCTCACACCAGACCGCAGCT
CCAGAGGTGAACAATATTTTCATCAAACAAGAACTTCCTACACCAGATCTTCATCTTTCT
GTCCCTACCCAGCAGGGCCACCTGTACCAGCTACTGAATACACCGGATCTAGATATGCCC
AGTTCTACAAATCAGACAGCAGCAATGGACACTCTTAATGTTTCTATGTCAGCTGCCATG
GCAGGCCTTAACACACACACCTCTGCTGTTCCGCAGACTGCAGTGAAACAATTCCAGGGC
ATGCCCCCTTGCACATACACAATGCCAAGTCAGTTTCTTCCACAACAGGCCACTTACTTT
CCCCCGTCACCACCAAGCTCAGAGCCTGGAAGTCCAGATAGACAAGCAGAGATGCTCCAG
AATTTAACCCCACCTCCATCCTATGCTGCTACAATTGCTTCTAAACTGGCAATTCACAAT
CCAAATTTACCCACCACCCTGCCAGTTAACTCACAAAACATCCAACCTGTCAGATACAAT
AGAAGGAGTAACCCCGATTTGGAGAAACGACGCATCCACTACTGCGATTACCCTGGTTGC
ACAAAAGTTTATACCGAGTCTTCTCATTTAAAAGCTCACCTGAGGACTCACACTGGTGAA
AAGCCATACAAGTGTACCTGGGAAGGCTGCGACTGGAGGTTCGCGCGATCGGATGAGCTG
ACCCGCCACTACCGGAAGCACACAGGCGCCAAGCCCTTCCAGTGCGGGGTGTGCAACCGC
AGCTTCTCGCGCTCTGACCACCTGGCCCTGCATATGAAGAGGCACCAGAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1688
Start on reference sequence 315
Mutations:
None
Discrepancy:
a1156g,K386E
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLF5
Symbol Nomenclature : KLF5
SYNONYM : BTEB2; CKLF; IKLF
Designation : BTE-binding protein 2|GC box binding protein 2|Krueppel-like factor 5|basic transcription element binding protein 2|colon krueppel-like factor|colon kruppel-like factor|intestinal-enriched krueppel-like factor|intestinal-enriched kruppel-like factor|transcription factor BTEB2
Full Nomenclature : Kruppel-like factor 5 (intestinal)
GENEID : 688
Locus Tag : RP11-505F3.5
HGNC : 6349
MIM : 602903
Vega : OTTHUMG00000017074
Target GenBank: BC007695

 

Reference Sequence Alignment

  

HsCD00329584      ATGGCTACAAGGGTGCTGAGCATGAGCGCCCGCCTGGGACCCGTGCCCCAGCCGCCGGCG
NM_001730.4       ATGGCTACAAGGGTGCTGAGCATGAGCGCCCGCCTGGGACCCGTGCCCCAGCCGCCGGCG
                  ************************************************************

HsCD00329584      CCGCAGGACGAGCCGGTGTTCGCGCAGCTCAAGCCGGTGCTGGGCGCCGCGAATCCGGCC
NM_001730.4       CCGCAGGACGAGCCGGTGTTCGCGCAGCTCAAGCCGGTGCTGGGCGCCGCGAATCCGGCC
                  ************************************************************

HsCD00329584      CGCGACGCGGCGCTCTTCCCCGGCGAGGAGCTGAAGCACGCGCACCACCGCCCGCAGGCG
NM_001730.4       CGCGACGCGGCGCTCTTCCCCGGCGAGGAGCTGAAGCACGCGCACCACCGCCCGCAGGCG
                  ************************************************************

HsCD00329584      CAGCCCGCGCCCGCGCAGGCCCCGCAGCCGGCCCAGCCGCCCGCCACCGGCCCGCGGCTG
NM_001730.4       CAGCCCGCGCCCGCGCAGGCCCCGCAGCCGGCCCAGCCGCCCGCCACCGGCCCGCGGCTG
                  ************************************************************

HsCD00329584      CCTCCAGAGGACCTGGTCCAGACAAGATGTGAAATGGAGAAGTATCTGACACCTCAGCTT
NM_001730.4       CCTCCAGAGGACCTGGTCCAGACAAGATGTGAAATGGAGAAGTATCTGACACCTCAGCTT
                  ************************************************************

HsCD00329584      CCTCCAGTTCCTATAATTCCAGAGCATAAAAAGTATAGACGAGACAGTGCCTCAGTCGTA
NM_001730.4       CCTCCAGTTCCTATAATTCCAGAGCATAAAAAGTATAGACGAGACAGTGCCTCAGTCGTA
                  ************************************************************

HsCD00329584      GACCAGTTCTTCACTGACACTGAAGGGTTACCTTACAGTATCAACATGAACGTCTTCCTC
NM_001730.4       GACCAGTTCTTCACTGACACTGAAGGGTTACCTTACAGTATCAACATGAACGTCTTCCTC
                  ************************************************************

HsCD00329584      CCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCGTGCGTAACACAC
NM_001730.4       CCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGACCGTGCGTAACACAC
                  ************************************************************

HsCD00329584      ATCAAGACAGAACCTGTTGCCATTTTCAGCCACCAGAGTGAAACGACTGCCCCTCCTCCG
NM_001730.4       ATCAAGACAGAACCTGTTGCCATTTTCAGCCACCAGAGTGAAACGACTGCCCCTCCTCCG
                  ************************************************************

HsCD00329584      GCCCCGACCCAGGCCCTCCCTGAGTTCACCAGTATATTCAGCTCACACCAGACCGCAGCT
NM_001730.4       GCCCCGACCCAGGCCCTCCCTGAGTTCACCAGTATATTCAGCTCACACCAGACCGCAGCT
                  ************************************************************

HsCD00329584      CCAGAGGTGAACAATATTTTCATCAAACAAGAACTTCCTACACCAGATCTTCATCTTTCT
NM_001730.4       CCAGAGGTGAACAATATTTTCATCAAACAAGAACTTCCTACACCAGATCTTCATCTTTCT
                  ************************************************************

HsCD00329584      GTCCCTACCCAGCAGGGCCACCTGTACCAGCTACTGAATACACCGGATCTAGATATGCCC
NM_001730.4       GTCCCTACCCAGCAGGGCCACCTGTACCAGCTACTGAATACACCGGATCTAGATATGCCC
                  ************************************************************

HsCD00329584      AGTTCTACAAATCAGACAGCAGCAATGGACACTCTTAATGTTTCTATGTCAGCTGCCATG
NM_001730.4       AGTTCTACAAATCAGACAGCAGCAATGGACACTCTTAATGTTTCTATGTCAGCTGCCATG
                  ************************************************************

HsCD00329584      GCAGGCCTTAACACACACACCTCTGCTGTTCCGCAGACTGCAGTGAAACAATTCCAGGGC
NM_001730.4       GCAGGCCTTAACACACACACCTCTGCTGTTCCGCAGACTGCAGTGAAACAATTCCAGGGC
                  ************************************************************

HsCD00329584      ATGCCCCCTTGCACATACACAATGCCAAGTCAGTTTCTTCCACAACAGGCCACTTACTTT
NM_001730.4       ATGCCCCCTTGCACATACACAATGCCAAGTCAGTTTCTTCCACAACAGGCCACTTACTTT
                  ************************************************************

HsCD00329584      CCCCCGTCACCACCAAGCTCAGAGCCTGGAAGTCCAGATAGACAAGCAGAGATGCTCCAG
NM_001730.4       CCCCCGTCACCACCAAGCTCAGAGCCTGGAAGTCCAGATAGACAAGCAGAGATGCTCCAG
                  ************************************************************

HsCD00329584      AATTTAACCCCACCTCCATCCTATGCTGCTACAATTGCTTCTAAACTGGCAATTCACAAT
NM_001730.4       AATTTAACCCCACCTCCATCCTATGCTGCTACAATTGCTTCTAAACTGGCAATTCACAAT
                  ************************************************************

HsCD00329584      CCAAATTTACCCACCACCCTGCCAGTTAACTCACAAAACATCCAACCTGTCAGATACAAT
NM_001730.4       CCAAATTTACCCACCACCCTGCCAGTTAACTCACAAAACATCCAACCTGTCAGATACAAT
                  ************************************************************

HsCD00329584      AGAAGGAGTAACCCCGATTTGGAGAAACGACGCATCCACTACTGCGATTACCCTGGTTGC
NM_001730.4       AGAAGGAGTAACCCCGATTTGGAGAAACGACGCATCCACTACTGCGATTACCCTGGTTGC
                  ************************************************************

HsCD00329584      ACAAAAGTTTATACCGAGTCTTCTCATTTAAAAGCTCACCTGAGGACTCACACTGGTGAA
NM_001730.4       ACAAAAGTTTATACCAAGTCTTCTCATTTAAAAGCTCACCTGAGGACTCACACTGGTGAA
                  ***************.********************************************

HsCD00329584      AAGCCATACAAGTGTACCTGGGAAGGCTGCGACTGGAGGTTCGCGCGATCGGATGAGCTG
NM_001730.4       AAGCCATACAAGTGTACCTGGGAAGGCTGCGACTGGAGGTTCGCGCGATCGGATGAGCTG
                  ************************************************************

HsCD00329584      ACCCGCCACTACCGGAAGCACACAGGCGCCAAGCCCTTCCAGTGCGGGGTGTGCAACCGC
NM_001730.4       ACCCGCCACTACCGGAAGCACACAGGCGCCAAGCCCTTCCAGTGCGGGGTGTGCAACCGC
                  ************************************************************

HsCD00329584      AGCTTCTCGCGCTCTGACCACCTGGCCCTGCATATGAAGAGGCACCAGAACTTG
NM_001730.4       AGCTTCTCGCGCTCTGACCACCTGGCCCTGCATATGAAGAGGCACCAGAACTGA
                  **************************************************** .

Pathways

Reactome : Developmental Biology
Reactome : Transcriptional regulation of white adipocyte differentiation
WikiPathway : Adipogenesis

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q13887
UniProt : Q5T6X2
HPRD : 04212

Clone

Cloning Information : 269086
HIP Master Clone ID : 137316
Original Clone ID : FLH269086.01L

Organism


TAX_ID : 9606
Species Specific ID: 688

Genome

Chromosome : 13
Map Location : 13q22.1
Ensembl : ENSG00000102554

Reagents

Labome : KLF5-antibody