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ETV3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ETV3
Gene Name:  ets variant 3
Sequence             
Original Clone ID: FLH269088.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329586

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 432nts         Open reading frame : 1 to 432
Reference Sequence Alignment

ATGAAAGCCGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCT
GACTGGGCCTACAAAACAGAGTCATCCCCAGGCTCCCGGCAGATCCAGCTGTGGCACTTC
ATCCTGGAGCTGCTGCAGAAGGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAG
TACGGGGAATTTGTCATCAAGGATCCAGATGAGGTGGCCCGCCTCTGGGGCCGCAGGAAA
TGCAAACCACAGATGAATTATGACAAGCTGAGCCGGGCCCTCAGATACTATTACAACAAG
AGGATCCTTCATAAAACAAAAGGGAAAAGATTTACCTATAAATTTAACTTCAACAAGCTG
GTGATGCCCAACTACCCATTCATCAACATTCGGTCAAGTGGTAAGATACAAACTCTTTTG
GTAGGGAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 519
Start on reference sequence 88
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ETV3
Symbol Nomenclature : ETV3
SYNONYM : METS; PE-1; PE1; bA110J1.4
Designation : ETS domain transcriptional repressor PE1|ETS translocation variant 3|ets variant gene 3, ETS family transcriptional repressor|mitogenic Ets transcriptional suppressor
Full Nomenclature : ets variant 3
GENEID : 2117
HGNC : 3492
MIM : 164873
Vega : OTTHUMG00000034298
Target GenBank: BC022868

 

Reference Sequence Alignment

  

HsCD00329586        ATGAAAGCCGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCT
NM_005240.1         ATGAAAGCCGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCT
NM_001145312.1      ATGAAAGCCGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCT
                    ************************************************************

HsCD00329586        GACTGGGCCTACAAAACAGAGTCATCCCCAGGCTCCCGGCAGATCCAGCTGTGGCACTTC
NM_005240.1         GACTGGGCCTACAAAACAGAGTCATCCCCAGGCTCCCGGCAGATCCAGCTGTGGCACTTC
NM_001145312.1      GACTGGGCCTACAAAACAGAGTCATCCCCAGGCTCCCGGCAGATCCAGCTGTGGCACTTC
                    ************************************************************

HsCD00329586        ATCCTGGAGCTGCTGCAGAAGGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAG
NM_005240.1         ATCCTGGAGCTGCTGCAGAAGGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAG
NM_001145312.1      ATCCTGGAGCTGCTGCAGAAGGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAG
                    ************************************************************

HsCD00329586        TACGGGGAATTTGTCATCAAGGATCCAGATGAGGTGGCCCGCCTCTGGGGCCGCAGGAAA
NM_005240.1         TACGGGGAATTTGTCATCAAGGATCCAGATGAGGTGGCCCGCCTCTGGGGCCGCAGGAAA
NM_001145312.1      TACGGGGAATTTGTCATCAAGGATCCAGATGAGGTGGCCCGCCTCTGGGGCCGCAGGAAA
                    ************************************************************

HsCD00329586        TGCAAACCACAGATGAATTATGACAAGCTGAGCCGGGCCCTCAGATACTATTACAACAAG
NM_005240.1         TGCAAACCACAGATGAATTATGACAAGCTGAGCCGGGCCCTCAGATACTATTACAACAAG
NM_001145312.1      TGCAAACCACAGATGAATTATGACAAGCTGAGCCGGGCCCTCAGATACTATTACAACAAG
                    ************************************************************

HsCD00329586        AGGATCCTTCATAAAACAAAAGGGAAAAGATTTACCTATAAATTTAACTTCAACAAGCTG
NM_005240.1         AGGATCCTTCATAAAACAAAAGGGAAAAGATTTACCTATAAATTTAACTTCAACAAGCTG
NM_001145312.1      AGGATCCTTCATAAAACAAAAGGGAAAAGATTTACCTATAAATTTAACTTCAACAAGCTG
                    ************************************************************

HsCD00329586        GTGATGCCCAACTACCCATTCATCAACATTCGGTCAAGTGGTAAGATACAAACTCTTTTG
NM_005240.1         GTGATGCCCAACTACCCATTCATCAACATTCGGTCAAGTGGTAAGATACAAACTCTTTTG
NM_001145312.1      GTGATGCCCAACTACCCATTCATCAACATTCGGTCAAGTGGTGTGGTTCCTCAGAGTGCA
                    ******************************************.:*.*:*.:.. . *  .

HsCD00329586        GTAGGGAATTTG------------------------------------------------
NM_005240.1         GTAGGGAATTAA------------------------------------------------
NM_001145312.1      CCACCAGTGCCAACAGCCTCTTCCCGGTTCCATTTCCCACCTCTGGACACCCATTCTCCA
                      *  ..:   .                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      ACCAATGATGTGCAGCCGGGACGGTTCTCTGCTAGCTCCCTAACTGCTTCTGGCCAGGAG
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      TCCAGTAATGGTACTGATAGAAAGACTGAGCTTTCAGAGCTGGAGGATGGCTCAGCTGCT
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GACTGGCGCCGGGGTGTGGATCCCGTGTCCTCCAGGAATGCCATTGGTGGAGGAGGGATT
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GGCCATCAGAAACGCAAGCCTGACATAATGCTTCCTCTGTTTGCTAGGCCAGGGATGTAC
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      CCTGACCCCCACAGTCCCTTCGCTGTCTCTCCAATCCCTGGCCGCGGAGGTGTCCTTAAT
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GTCCCCATTTCACCAGCCCTCTCCCTGACTCCCACCATCTTCTCCTATAGCCCATCACCA
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GGCCTGAGCCCTTTCACCAGCAGCAGCTGCTTCTCCTTCAACCCTGAGGAAATGAAACAC
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      TACCTTCATTCTCAAGCTTGTTCTGTGTTCAACTACCATCTGAGTCCTCGGACTTTTCCC
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      CGTTACCCAGGGCTCATGGTTCCACCACTGCAGTGCCAAATGCATCCTGAGGAGTCAACT
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      CAGTTCTCCATCAAGCTGCAGCCCCCACCAGTTGGGCGGAAGAACCGGGAGAGGGTTGAG
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      AGCAGCGAGGAGTCAGCACCAGTCACCACGCCCACCATGGCTTCTATTCCCCCAAGAATC
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      AAGGTGGAACCTGCCTCTGAAAAGGATCCTGAGAGCCTCAGGCAGTCGGCACGAGAGAAG
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GAGGAGCACACTCAAGAAGAGGGCACTGTGCCAAGCAGGACCATTGAAGAGGAAAAAGGC
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      ACCATCTTTGCCCGTCCTGCTGCACCACCCATCTGGCCCTCTGTGCCCATTAGCACCCCA
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      AGTGGAGAACCTCTGGAGGTGACTGAAGACAGTGAGGATAGGCCTGGCAAAGAGCCCAGT
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      GCACCTGAGAAGAAAGAAGATGCACTGATGCCCCCCAAGCTTCGGTTGAAGCGGCGCTGG
                                                                                

HsCD00329586        ------------------------------------------------------------
NM_005240.1         ------------------------------------------------------------
NM_001145312.1      AATGATGACCCTGAAGCCCGAGAGCTGAGCAAGAGTGGCAAGTTTCTCTGGAATGGGTCA
                                                                                

HsCD00329586        ---------------------------------------
NM_005240.1         ---------------------------------------
NM_001145312.1      GGACCCCAGGGCTTGGCAACAGCAGCTGCTGATGCTTAG
                                                           

Pathways

Panther : PDGF signaling pathway

Protein

SMART domain : ETS : erythroblast transformation specific domain
UniProt : P41162
UniProt : B4E3M7
HPRD : 01282

Clone

Cloning Information : 269088
HIP Master Clone ID : 169125
Original Clone ID : FLH269088.01L

Organism


TAX_ID : 9606
Species Specific ID: 2117

Genome

Chromosome : 1
Map Location : 1q21-q23
Ensembl : ENSG00000117036

Reagents

Labome : PE-1-antibody