DNASU Plasmid Repository • 480.965.5697 | Email

SOX10 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SOX10
Gene Name:  SRY (sex determining region Y)-box 10
Sequence             
Original Clone ID: FLH269090.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329588

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1401nts         Open reading frame : 1 to 1401
Reference Sequence Alignment

ATGGCGGAGGAGCAGGACCTATCGGAGGTGGAGCTGAGCCCCGTGGGCTCGGAGGAGCCC
CGCTGCCTGTCCCCGGGGAGCGCGCCCTCGCTAGGGCCCGACGGCGGCGGCGGCGGATCG
GGCCTGCGAGCCAGCCCGGGGCCAGGCGAGCTGGGCAAGGTCAAGAAGGAGCAGCAGGAC
GGCGAGGCGGACGATGACAAGTTCCCCGTGTGCATCCGCGAGGCCGTCAGCCAGGTGCTC
AGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCGTCAACGGCGCCAGCAAAAGC
AAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCTCAGGCAGCGCGCAGG
AAGCTCGCGGACCAGTACCCGCACCTGCACAACGCTGAGCTCAGCAAGACGCTGGGCAAG
CTCTGGAGGCTGCTGAACGAAAGTGACAAGCGCCCCTTCATCGAGGAGGCTGAGCGGCTC
CGTATGCAGCACAAGAAAGACCACCCGGACTACAAGTACCAGCCCAGGCGGCGGAAGAAC
GGGAAGGCCGCCCAGGGCGAGGCGGAGTGCCCCGGTGGGGAGGCCGAGCAAGGTGGGACC
GCCGCCATCCAGGCCCACTACAAGAGCGCCCACTTGGACCACCGGCACCCAGGAGAGGGC
TCCCCCATGTCAGATGGGAACCCCGAGCACCCCTCAGGCCAGAGCCATGGCCCACCCACC
CCTCCAACCACCCCGAAGACAGAGCTGCAGTCGGGCAAGGCAGACCCGAAGCGGGACGGG
CGCTCCATGGGGGAGGGCGGGAAGCCTCACATCGACTTCGGCAACGTGGACATTGGTGAG
ATCAGCCACGAGGTAATGTCCAACATGGAGACCTTTGATGTGGCTGAGTTGGACCAGTAC
CTGCCGCCCAATGGGCACCCAGGCCATGTGAGCAGCTACTCAGCAGCCGGCTATGGGCTG
GGCAGTGCCCTGGCCGTGGCCAGTGGACACTCCGCCTGGATCTCCAAGCCACCAGGCGTG
GCTCTGCCCACGGTCTCACCACCTGGTGTGGATGCCAAAGCCCAGGTGAAGACAGAGACC
GCGGGGCCCCAGGGGCCCCCACACTACACCGACCAGCCATCCACCTCACAGATCGCCTAC
ACCTCCCTCAGCCTGCCCCACTATGGCTCAGCCTTCCCCTCCATCTCCCGCCCCCAGTTT
GACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACTCGGGCCAGGCCTCTGGC
CTCTACTCGGCCTTCTCCTATATGGGGCCCTCGCAGCGGCCCCTCTACACGGCCATCTCT
GACCCCAGCCCCTCAGGGCCCCAGTCCCACAGCCCCACACACTGGGAGCAGCCAGTATAT
ACGACACTGTCCCGGCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1502
Start on reference sequence 102
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SOX10
Symbol Nomenclature : SOX10
SYNONYM : DOM; PCWH; WS2E; WS4; WS4C
Designation : SRY-related HMG-box gene 10|dominant megacolon, mouse, human homolog of|transcription factor SOX-10
Full Nomenclature : SRY (sex determining region Y)-box 10
GENEID : 6663
Locus Tag : RP5-1039K5.9
HGNC : 11190
MIM : 602229
Vega : OTTHUMG00000149913
Target GenBank: BC002824

 

Reference Sequence Alignment

  

HsCD00329588      ATGGCGGAGGAGCAGGACCTATCGGAGGTGGAGCTGAGCCCCGTGGGCTCGGAGGAGCCC
NM_006941.3       ATGGCGGAGGAGCAGGACCTATCGGAGGTGGAGCTGAGCCCCGTGGGCTCGGAGGAGCCC
                  ************************************************************

HsCD00329588      CGCTGCCTGTCCCCGGGGAGCGCGCCCTCGCTAGGGCCCGACGGCGGCGGCGGCGGATCG
NM_006941.3       CGCTGCCTGTCCCCGGGGAGCGCGCCCTCGCTAGGGCCCGACGGCGGCGGCGGCGGATCG
                  ************************************************************

HsCD00329588      GGCCTGCGAGCCAGCCCGGGGCCAGGCGAGCTGGGCAAGGTCAAGAAGGAGCAGCAGGAC
NM_006941.3       GGCCTGCGAGCCAGCCCGGGGCCAGGCGAGCTGGGCAAGGTCAAGAAGGAGCAGCAGGAC
                  ************************************************************

HsCD00329588      GGCGAGGCGGACGATGACAAGTTCCCCGTGTGCATCCGCGAGGCCGTCAGCCAGGTGCTC
NM_006941.3       GGCGAGGCGGACGATGACAAGTTCCCCGTGTGCATCCGCGAGGCCGTCAGCCAGGTGCTC
                  ************************************************************

HsCD00329588      AGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCGTCAACGGCGCCAGCAAAAGC
NM_006941.3       AGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCGTCAACGGCGCCAGCAAAAGC
                  ************************************************************

HsCD00329588      AAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCTCAGGCAGCGCGCAGG
NM_006941.3       AAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCTCAGGCAGCGCGCAGG
                  ************************************************************

HsCD00329588      AAGCTCGCGGACCAGTACCCGCACCTGCACAACGCTGAGCTCAGCAAGACGCTGGGCAAG
NM_006941.3       AAGCTCGCGGACCAGTACCCGCACCTGCACAACGCTGAGCTCAGCAAGACGCTGGGCAAG
                  ************************************************************

HsCD00329588      CTCTGGAGGCTGCTGAACGAAAGTGACAAGCGCCCCTTCATCGAGGAGGCTGAGCGGCTC
NM_006941.3       CTCTGGAGGCTGCTGAACGAAAGTGACAAGCGCCCCTTCATCGAGGAGGCTGAGCGGCTC
                  ************************************************************

HsCD00329588      CGTATGCAGCACAAGAAAGACCACCCGGACTACAAGTACCAGCCCAGGCGGCGGAAGAAC
NM_006941.3       CGTATGCAGCACAAGAAAGACCACCCGGACTACAAGTACCAGCCCAGGCGGCGGAAGAAC
                  ************************************************************

HsCD00329588      GGGAAGGCCGCCCAGGGCGAGGCGGAGTGCCCCGGTGGGGAGGCCGAGCAAGGTGGGACC
NM_006941.3       GGGAAGGCCGCCCAGGGCGAGGCGGAGTGCCCCGGTGGGGAGGCCGAGCAAGGTGGGACC
                  ************************************************************

HsCD00329588      GCCGCCATCCAGGCCCACTACAAGAGCGCCCACTTGGACCACCGGCACCCAGGAGAGGGC
NM_006941.3       GCCGCCATCCAGGCCCACTACAAGAGCGCCCACTTGGACCACCGGCACCCAGGAGAGGGC
                  ************************************************************

HsCD00329588      TCCCCCATGTCAGATGGGAACCCCGAGCACCCCTCAGGCCAGAGCCATGGCCCACCCACC
NM_006941.3       TCCCCCATGTCAGATGGGAACCCCGAGCACCCCTCAGGCCAGAGCCATGGCCCACCCACC
                  ************************************************************

HsCD00329588      CCTCCAACCACCCCGAAGACAGAGCTGCAGTCGGGCAAGGCAGACCCGAAGCGGGACGGG
NM_006941.3       CCTCCAACCACCCCGAAGACAGAGCTGCAGTCGGGCAAGGCAGACCCGAAGCGGGACGGG
                  ************************************************************

HsCD00329588      CGCTCCATGGGGGAGGGCGGGAAGCCTCACATCGACTTCGGCAACGTGGACATTGGTGAG
NM_006941.3       CGCTCCATGGGGGAGGGCGGGAAGCCTCACATCGACTTCGGCAACGTGGACATTGGTGAG
                  ************************************************************

HsCD00329588      ATCAGCCACGAGGTAATGTCCAACATGGAGACCTTTGATGTGGCTGAGTTGGACCAGTAC
NM_006941.3       ATCAGCCACGAGGTAATGTCCAACATGGAGACCTTTGATGTGGCTGAGTTGGACCAGTAC
                  ************************************************************

HsCD00329588      CTGCCGCCCAATGGGCACCCAGGCCATGTGAGCAGCTACTCAGCAGCCGGCTATGGGCTG
NM_006941.3       CTGCCGCCCAATGGGCACCCAGGCCATGTGAGCAGCTACTCAGCAGCCGGCTATGGGCTG
                  ************************************************************

HsCD00329588      GGCAGTGCCCTGGCCGTGGCCAGTGGACACTCCGCCTGGATCTCCAAGCCACCAGGCGTG
NM_006941.3       GGCAGTGCCCTGGCCGTGGCCAGTGGACACTCCGCCTGGATCTCCAAGCCACCAGGCGTG
                  ************************************************************

HsCD00329588      GCTCTGCCCACGGTCTCACCACCTGGTGTGGATGCCAAAGCCCAGGTGAAGACAGAGACC
NM_006941.3       GCTCTGCCCACGGTCTCACCACCTGGTGTGGATGCCAAAGCCCAGGTGAAGACAGAGACC
                  ************************************************************

HsCD00329588      GCGGGGCCCCAGGGGCCCCCACACTACACCGACCAGCCATCCACCTCACAGATCGCCTAC
NM_006941.3       GCGGGGCCCCAGGGGCCCCCACACTACACCGACCAGCCATCCACCTCACAGATCGCCTAC
                  ************************************************************

HsCD00329588      ACCTCCCTCAGCCTGCCCCACTATGGCTCAGCCTTCCCCTCCATCTCCCGCCCCCAGTTT
NM_006941.3       ACCTCCCTCAGCCTGCCCCACTATGGCTCAGCCTTCCCCTCCATCTCCCGCCCCCAGTTT
                  ************************************************************

HsCD00329588      GACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACTCGGGCCAGGCCTCTGGC
NM_006941.3       GACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACTCGGGCCAGGCCTCTGGC
                  ************************************************************

HsCD00329588      CTCTACTCGGCCTTCTCCTATATGGGGCCCTCGCAGCGGCCCCTCTACACGGCCATCTCT
NM_006941.3       CTCTACTCGGCCTTCTCCTATATGGGGCCCTCGCAGCGGCCCCTCTACACGGCCATCTCT
                  ************************************************************

HsCD00329588      GACCCCAGCCCCTCAGGGCCCCAGTCCCACAGCCCCACACACTGGGAGCAGCCAGTATAT
NM_006941.3       GACCCCAGCCCCTCAGGGCCCCAGTCCCACAGCCCCACACACTGGGAGCAGCCAGTATAT
                  ************************************************************

HsCD00329588      ACGACACTGTCCCGGCCCTTG
NM_006941.3       ACGACACTGTCCCGGCCCTAA
                  *******************:.

Pathways

WikiPathway : Neural Crest Differentiation

Protein

SMART domain : HMG : high mobility group
UniProt : P56693
HPRD : 03752

Clone

Cloning Information : 269090
HIP Master Clone ID : 169322
Original Clone ID : FLH269090.01L

Organism


TAX_ID : 9606
Species Specific ID: 6663

Genome

Chromosome : 22
Map Location : 22q13.1
Ensembl : ENSG00000100146

Reagents

Labome : SOX10-antibody