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CSRP2BP (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CSRP2BP
Gene Name:  CSRP2 binding protein
Sequence             
Original Clone ID: FLH269091.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329589

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2349nts         Open reading frame : 1 to 2349
Reference Sequence Alignment

ATGGATAGTAGCATCCACCTGAGTAGTCTGATCAGTCGGCATGATGACGAAGCCACGAGA
ACATCGACCTCAGAAGGACTGGAGGAAGGTGAAGTGGAGGGAGAGACGCTCCTGATCGTC
GAATCCGAGGATCAGGCATCAGTGGACTTATCGCACGACCAGAGTGGGGATTCCCTCAAC
AGTGATGAAGGAGACGTGTCTTGGATGGAGGAGCAGCTGTCCTACTTCTGTGACAAGTGC
CAAAAATGGATACCAGCCAGTCAGCTGAGGGAACAGCTCAGTTACCTTAAGGGTGATAAT
TTTTTTAGGTTTACTTGTTCGGATTGCTCAGCAGATGGCAAGGAGCAGTATGAAAGGCTG
AAGCTGACATGGCAGCAAGTCGTCATGTTGGCAATGTACAACTTGTCTCTGGAAGGAAGT
GGACGTCAAGGTTATTTCAGGTGGAAAGAAGATATCTGTGCTTTTATTGAGAAACATTGG
ACTTTTTTACTAGGGAATAGGAAAAAGACGTCTACCTGGTGGAGCACCGTGGCAGGTTGC
CTCAGCGTGGGAAGTCCCATGTACTTCCGTTCAGGTGCTCAGGAATTTGGAGAGCCAGGA
TGGTGGAAACTTGTTCATAACAAGCCCCCAACGATGAAACCTGAAGGAGAGAAGTTGTCT
GCCTCTACTTTGAAAATAAAAGCAGCCTCAAAACCAACTTTAGATCCCATCATTACTGTT
GAGGGACTTAGAAAACGAGCAAGTCGGAATCCTGTGGAATCTGCCATGGAATTAAAAGAG
AAAAGGTCTCGAACTCAGGAAGCAAAAGACATTAGAAGAGCCCAGAAGGAGGCCGCTGGC
TTTCTTGACAGGAGCACATCTTCTACCCCTGTAAAATTCATAAGCCGAGGCCGCAGGCCA
GATGTGATTCTGGAAAAAGGCGAAGTGATTGACTTTTCCTCCTTGAGCTCCTCTGACCGC
ACCCCGCTGACAAGCCCATCTCCTTCTCCTTCTCTGGATTTCTCTGCCCCTGGTACACCT
GCCTCTCATTCTGCCACACCTAGCTTGCTTTCAGAAGCAGATCTGATTCCAGATGTGATG
CCCCCACAAGCCTTGTTTCATGATGACGATGAGATGGAAGGCGATGGAGTCATAGACCCA
GGGATGGAGTACGTCCCACCCCCTGCTGGGTCAGTAGCTTCTGGGCCAGTGGTTGGGGGC
AGAAAGAAGGTCAGAGGCCCTGAACAGATAAAGCAGGAGGTAGAGAGTGAGGAGGAAAAA
CCCGACAGGATGGATATTGACAGTGAAGACACAGATTCAAACACATCTTTGCAAACAAGG
GCTAGAGAAAAGAGGAAGCCTCAGCTGGAGAAGGACACAAAGCCGAAAGAGCCCAGGTAT
ACTCCCGTGAGCATCTACGAGGAAAAGCTGCTGCTCAAGAGGCTGGAAGCTTGTCCCGGT
GCTGTTGCCATGACTCCGGAAGCTCGGAGACTGAAACGCAAACTGATTGTCAGACAAGCG
AAAAGGGATAGGGGATTACCACTTTTTGACTTGGATCAAGTTGTTAATGCTGCTCTTTTG
TTAGTTGACGGGATTTATGGAGCCAAAGAAGGAGGAATTTCCAGACTTCCAGCTGGACAA
GCCACGTACAGAACCACCTGTCAGGACTTCAGAATCCTTGACCGATACCAGACTTCCTTG
CCGTCCAGGAAGGGATTTCGACACCAGACCACCAAGTTTTTGTATCGCTTGGTAGGATCA
GAAGATATGGCTGTGGACCAGAGTATTGTCAGCCCTTATACCTCTCGGATCTTGAAACCT
TATATCAGGCGTGATTATGAAACAAAGCCACCCAAACTGCAGCTCCTGTCACAGATTCGT
TCCCACCTGCACAGGAGCGACCCTCACTGGACGCCGGAGCCCGACGCACCTCTCGATTAC
TGTTATGTGCGGCCAAATCACATCCCAACGATCAACTCCATGTGTCAGGAGTTTTTTTGG
CCTGGCATTGACCTGTCTGAGTGTCTGCAGTACCCAGACTTCAGTGTTGTTGTTCTTTAT
AAAAAAGTCATCATTGCCTTTGGCTTCATGGTTCCTGATGTGAAATACAATGAAGCTTAC
ATTTCATTTCTGTTCGTCCACCCTGAATGGAGAAGAGCAGGGATTGCAACTTTCATGATC
TATCATCTGATTCAGACCTGCATGGGCAAGGACGTAACCCTTCACGTCTCAGCAAGCAAC
CCCGCTATGCTACTGTACCAGAAGTTTGGATTCAAGACTGAAGAATATGTATTAGATTTC
TATGATAAATATTACCCATTGGAGAGTACAGAGTGTAAACACGCATTCTTTCTGAGGCTC
CGGCGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2690
Start on reference sequence 342
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSRP2BP
Symbol Nomenclature : CSRP2BP
SYNONYM : ATAC2; CRP2BP; KAT14; PRO1194; dJ717M23.1
Designation : ADA2A-containing complex subunit 2|ATAC component 2 homolog|CRP2 binding partner|CRP2 binding protein|CSRP2-binding protein|cysteine-rich protein 2-binding protein
Full Nomenclature : CSRP2 binding protein
GENEID : 57325
Locus Tag : RP4-717M23.1
HGNC : 15904
Vega : OTTHUMG00000031962
Target GenBank: BC007537

 

Reference Sequence Alignment

  

HsCD00329589      ATGGATAGTAGCATCCACCTGAGTAGTCTGATCAGTCGGCATGATGACGAAGCCACGAGA
NM_020536.4       ATGGATAGTAGCATCCACCTGAGTAGTCTGATCAGTCGGCATGATGACGAAGCCACGAGA
                  ************************************************************

HsCD00329589      ACATCGACCTCAGAAGGACTGGAGGAAGGTGAAGTGGAGGGAGAGACGCTCCTGATCGTC
NM_020536.4       ACATCGACCTCAGAAGGACTGGAGGAAGGTGAAGTGGAGGGAGAGACGCTCCTGATCGTC
                  ************************************************************

HsCD00329589      GAATCCGAGGATCAGGCATCAGTGGACTTATCGCACGACCAGAGTGGGGATTCCCTCAAC
NM_020536.4       GAATCCGAGGATCAGGCATCAGTGGACTTATCGCACGACCAGAGTGGGGATTCCCTCAAC
                  ************************************************************

HsCD00329589      AGTGATGAAGGAGACGTGTCTTGGATGGAGGAGCAGCTGTCCTACTTCTGTGACAAGTGC
NM_020536.4       AGTGATGAAGGAGACGTGTCTTGGATGGAGGAGCAGCTGTCCTACTTCTGTGACAAGTGC
                  ************************************************************

HsCD00329589      CAAAAATGGATACCAGCCAGTCAGCTGAGGGAACAGCTCAGTTACCTTAAGGGTGATAAT
NM_020536.4       CAAAAATGGATACCAGCCAGTCAGCTGAGGGAACAGCTCAGTTACCTTAAGGGTGATAAT
                  ************************************************************

HsCD00329589      TTTTTTAGGTTTACTTGTTCGGATTGCTCAGCAGATGGCAAGGAGCAGTATGAAAGGCTG
NM_020536.4       TTTTTTAGGTTTACTTGTTCGGATTGCTCAGCAGATGGCAAGGAGCAGTATGAAAGGCTG
                  ************************************************************

HsCD00329589      AAGCTGACATGGCAGCAAGTCGTCATGTTGGCAATGTACAACTTGTCTCTGGAAGGAAGT
NM_020536.4       AAGCTGACATGGCAGCAAGTCGTCATGTTGGCAATGTACAACTTGTCTCTGGAAGGAAGT
                  ************************************************************

HsCD00329589      GGACGTCAAGGTTATTTCAGGTGGAAAGAAGATATCTGTGCTTTTATTGAGAAACATTGG
NM_020536.4       GGACGTCAAGGTTATTTCAGGTGGAAAGAAGATATCTGTGCTTTTATTGAGAAACATTGG
                  ************************************************************

HsCD00329589      ACTTTTTTACTAGGGAATAGGAAAAAGACGTCTACCTGGTGGAGCACCGTGGCAGGTTGC
NM_020536.4       ACTTTTTTACTAGGGAATAGGAAAAAGACGTCTACCTGGTGGAGCACCGTGGCAGGTTGC
                  ************************************************************

HsCD00329589      CTCAGCGTGGGAAGTCCCATGTACTTCCGTTCAGGTGCTCAGGAATTTGGAGAGCCAGGA
NM_020536.4       CTCAGCGTGGGAAGTCCCATGTACTTCCGTTCAGGTGCTCAGGAATTTGGAGAGCCAGGA
                  ************************************************************

HsCD00329589      TGGTGGAAACTTGTTCATAACAAGCCCCCAACGATGAAACCTGAAGGAGAGAAGTTGTCT
NM_020536.4       TGGTGGAAACTTGTTCATAACAAGCCCCCAACGATGAAACCTGAAGGAGAGAAGTTGTCT
                  ************************************************************

HsCD00329589      GCCTCTACTTTGAAAATAAAAGCAGCCTCAAAACCAACTTTAGATCCCATCATTACTGTT
NM_020536.4       GCCTCTACTTTGAAAATAAAAGCAGCCTCAAAACCAACTTTAGATCCCATCATTACTGTT
                  ************************************************************

HsCD00329589      GAGGGACTTAGAAAACGAGCAAGTCGGAATCCTGTGGAATCTGCCATGGAATTAAAAGAG
NM_020536.4       GAGGGACTTAGAAAACGAGCAAGTCGGAATCCTGTGGAATCTGCCATGGAATTAAAAGAG
                  ************************************************************

HsCD00329589      AAAAGGTCTCGAACTCAGGAAGCAAAAGACATTAGAAGAGCCCAGAAGGAGGCCGCTGGC
NM_020536.4       AAAAGGTCTCGAACTCAGGAAGCAAAAGACATTAGAAGAGCCCAGAAGGAGGCCGCTGGC
                  ************************************************************

HsCD00329589      TTTCTTGACAGGAGCACATCTTCTACCCCTGTAAAATTCATAAGCCGAGGCCGCAGGCCA
NM_020536.4       TTTCTTGACAGGAGCACATCTTCTACCCCTGTAAAATTCATAAGCCGAGGCCGCAGGCCA
                  ************************************************************

HsCD00329589      GATGTGATTCTGGAAAAAGGCGAAGTGATTGACTTTTCCTCCTTGAGCTCCTCTGACCGC
NM_020536.4       GATGTGATTCTGGAAAAAGGCGAAGTGATTGACTTTTCCTCCTTGAGCTCCTCTGACCGC
                  ************************************************************

HsCD00329589      ACCCCGCTGACAAGCCCATCTCCTTCTCCTTCTCTGGATTTCTCTGCCCCTGGTACACCT
NM_020536.4       ACCCCGCTGACAAGCCCATCTCCTTCTCCTTCTCTGGATTTCTCTGCCCCTGGTACACCT
                  ************************************************************

HsCD00329589      GCCTCTCATTCTGCCACACCTAGCTTGCTTTCAGAAGCAGATCTGATTCCAGATGTGATG
NM_020536.4       GCCTCTCATTCTGCCACACCTAGCTTGCTTTCAGAAGCAGATCTGATTCCAGATGTGATG
                  ************************************************************

HsCD00329589      CCCCCACAAGCCTTGTTTCATGATGACGATGAGATGGAAGGCGATGGAGTCATAGACCCA
NM_020536.4       CCCCCACAAGCCTTGTTTCATGATGACGATGAGATGGAAGGCGATGGAGTCATAGACCCA
                  ************************************************************

HsCD00329589      GGGATGGAGTACGTCCCACCCCCTGCTGGGTCAGTAGCTTCTGGGCCAGTGGTTGGGGGC
NM_020536.4       GGGATGGAGTACGTCCCACCCCCTGCTGGGTCAGTAGCTTCTGGGCCAGTGGTTGGGGGC
                  ************************************************************

HsCD00329589      AGAAAGAAGGTCAGAGGCCCTGAACAGATAAAGCAGGAGGTAGAGAGTGAGGAGGAAAAA
NM_020536.4       AGAAAGAAGGTCAGAGGCCCTGAACAGATAAAGCAGGAGGTAGAGAGTGAGGAGGAAAAA
                  ************************************************************

HsCD00329589      CCCGACAGGATGGATATTGACAGTGAAGACACAGATTCAAACACATCTTTGCAAACAAGG
NM_020536.4       CCCGACAGGATGGATATTGACAGTGAAGACACAGATTCAAACACATCTTTGCAAACAAGG
                  ************************************************************

HsCD00329589      GCTAGAGAAAAGAGGAAGCCTCAGCTGGAGAAGGACACAAAGCCGAAAGAGCCCAGGTAT
NM_020536.4       GCTAGAGAAAAGAGGAAGCCTCAGCTGGAGAAGGACACAAAGCCGAAAGAGCCCAGGTAT
                  ************************************************************

HsCD00329589      ACTCCCGTGAGCATCTACGAGGAAAAGCTGCTGCTCAAGAGGCTGGAAGCTTGTCCCGGT
NM_020536.4       ACTCCCGTGAGCATCTACGAGGAAAAGCTGCTGCTCAAGAGGCTGGAAGCTTGTCCCGGT
                  ************************************************************

HsCD00329589      GCTGTTGCCATGACTCCGGAAGCTCGGAGACTGAAACGCAAACTGATTGTCAGACAAGCG
NM_020536.4       GCTGTTGCCATGACTCCGGAAGCTCGGAGACTGAAACGCAAACTGATTGTCAGACAAGCG
                  ************************************************************

HsCD00329589      AAAAGGGATAGGGGATTACCACTTTTTGACTTGGATCAAGTTGTTAATGCTGCTCTTTTG
NM_020536.4       AAAAGGGATAGGGGATTACCACTTTTTGACTTGGATCAAGTTGTTAATGCTGCTCTTTTG
                  ************************************************************

HsCD00329589      TTAGTTGACGGGATTTATGGAGCCAAAGAAGGAGGAATTTCCAGACTTCCAGCTGGACAA
NM_020536.4       TTAGTTGACGGGATTTATGGAGCCAAAGAAGGAGGAATTTCCAGACTTCCAGCTGGACAA
                  ************************************************************

HsCD00329589      GCCACGTACAGAACCACCTGTCAGGACTTCAGAATCCTTGACCGATACCAGACTTCCTTG
NM_020536.4       GCCACGTACAGAACCACCTGTCAGGACTTCAGAATCCTTGACCGATACCAGACTTCCTTG
                  ************************************************************

HsCD00329589      CCGTCCAGGAAGGGATTTCGACACCAGACCACCAAGTTTTTGTATCGCTTGGTAGGATCA
NM_020536.4       CCGTCCAGGAAGGGATTTCGACACCAGACCACCAAGTTTTTGTATCGCTTGGTAGGATCA
                  ************************************************************

HsCD00329589      GAAGATATGGCTGTGGACCAGAGTATTGTCAGCCCTTATACCTCTCGGATCTTGAAACCT
NM_020536.4       GAAGATATGGCTGTGGACCAGAGTATTGTCAGCCCTTATACCTCTCGGATCTTGAAACCT
                  ************************************************************

HsCD00329589      TATATCAGGCGTGATTATGAAACAAAGCCACCCAAACTGCAGCTCCTGTCACAGATTCGT
NM_020536.4       TATATCAGGCGTGATTATGAAACAAAGCCACCCAAACTGCAGCTCCTGTCACAGATTCGT
                  ************************************************************

HsCD00329589      TCCCACCTGCACAGGAGCGACCCTCACTGGACGCCGGAGCCCGACGCACCTCTCGATTAC
NM_020536.4       TCCCACCTGCACAGGAGCGACCCTCACTGGACGCCGGAGCCCGACGCACCTCTCGATTAC
                  ************************************************************

HsCD00329589      TGTTATGTGCGGCCAAATCACATCCCAACGATCAACTCCATGTGTCAGGAGTTTTTTTGG
NM_020536.4       TGTTATGTGCGGCCAAATCACATCCCAACGATCAACTCCATGTGTCAGGAGTTTTTTTGG
                  ************************************************************

HsCD00329589      CCTGGCATTGACCTGTCTGAGTGTCTGCAGTACCCAGACTTCAGTGTTGTTGTTCTTTAT
NM_020536.4       CCTGGCATTGACCTGTCTGAGTGTCTGCAGTACCCAGACTTCAGTGTTGTTGTTCTTTAT
                  ************************************************************

HsCD00329589      AAAAAAGTCATCATTGCCTTTGGCTTCATGGTTCCTGATGTGAAATACAATGAAGCTTAC
NM_020536.4       AAAAAAGTCATCATTGCCTTTGGCTTCATGGTTCCTGATGTGAAATACAATGAAGCTTAC
                  ************************************************************

HsCD00329589      ATTTCATTTCTGTTCGTCCACCCTGAATGGAGAAGAGCAGGGATTGCAACTTTCATGATC
NM_020536.4       ATTTCATTTCTGTTCGTCCACCCTGAATGGAGAAGAGCAGGGATTGCAACTTTCATGATC
                  ************************************************************

HsCD00329589      TATCATCTGATTCAGACCTGCATGGGCAAGGACGTAACCCTTCACGTCTCAGCAAGCAAC
NM_020536.4       TATCATCTGATTCAGACCTGCATGGGCAAGGACGTAACCCTTCACGTCTCAGCAAGCAAC
                  ************************************************************

HsCD00329589      CCCGCTATGCTACTGTACCAGAAGTTTGGATTCAAGACTGAAGAATATGTATTAGATTTC
NM_020536.4       CCCGCTATGCTACTGTACCAGAAGTTTGGATTCAAGACTGAAGAATATGTATTAGATTTC
                  ************************************************************

HsCD00329589      TATGATAAATATTACCCATTGGAGAGTACAGAGTGTAAACACGCATTCTTTCTGAGGCTC
NM_020536.4       TATGATAAATATTACCCATTGGAGAGTACAGAGTGTAAACACGCATTCTTTCTGAGGCTC
                  ************************************************************

HsCD00329589      CGGCGCTTG
NM_020536.4       CGGCGCTGA
                  ******* .

Pathways

Reactome : Chromatin modifying enzymes
Reactome : Chromatin organization
Reactome : HATs acetylate histones

Protein

UniProt : Q9H8E8
HPRD : 16765

Clone

Cloning Information : 269091
HIP Master Clone ID : 169702
Original Clone ID : FLH269091.01L

Organism


TAX_ID : 9606
Species Specific ID: 57325

Genome

Chromosome : 20
Map Location : 20p11.23
Ensembl : ENSG00000149474

Reagents

Labome : CRP2BP-antibody