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DDX24 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DDX24
Gene Name:  DEAD (Asp-Glu-Ala-Asp) box helicase 24
Sequence             
Original Clone ID: FLH269098.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329596

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2580nts         Open reading frame : 1 to 2580
Reference Sequence Alignment

ATGAAGTTGAAGGACACAAAATCAAGGCCAAAGCAGTCAAGCTGTGGCAAATTTCAGACA
AAGGGAATCAAAGTTGTGGGAAAATGGAAGGAAGTGAAGATTGACCCAAATATGTTTGCA
GATGGACAGATGGATGACTTGGTGTGCTTTGAGGAATTGACAGATTACCAGTTGGTCTCC
CCTGCCAAGAATCCCTCCAGTCTCTTCTCAAAGGAAGCACCCAAGAGAAAGGCACAAGCT
GTTTCAGAAGAAGAGGAGGAGGAGGAGGGAAAGTCTAGCTCACCAAAGAAAAAGATCAAG
TTGAAGAAAAGTAAAAATGTAGCAACTGAAGGAACCAGTACCCAGAAAGAATTTGAAGTG
AAAGATCCTGAGCTGGAGGCCCAGGGAGATGACATGGTTTGTGATGATCCGGAGGCTGGG
GAGATGACATCAGAAAACCTGGTCCAAACTGCTCCAAAAAAGAAGAAAAATAAAGGGAAA
AAAGGGTTGGAGCCTTCTCAGAGCACTGCTGCCAAGGTGCCCAAAAAAGCGAAGACATGG
ATTCCTGAAGTTCATGATCAGAAAGCAGATGTGTCAGCTTGGAAGGACCTGTTTGTTCCC
AGGCCGGTTCTCCGAGCACTCAGCTTTCTAGGCTTCTCTGCACCCACACCAATCCAAGCC
CTGACCTTGGCACCTGCCATCCGTGACAAACTGGACATCCTTGGGGCTGCTGAGACAGGA
AGTGGGAAAACTCTTGCCTTTGCCATCCCAATGATTCATGCGGTGTTGCAGTGGCAGAAG
AGGAATGCTGCCCCTCCTCCAAGTAACACCGAAGCACCACCTGGAGAGACCAGAACTGAG
GCCGGAGCTGAGACTAGATCACCAGGCAAGGCTGAAGCTGAGTCTGATGCATTGCCTGAC
GATACTGTAATTGAGAGTGAAGCACTGCCCAGTGATATTGCAGCCGAGGCCAGAGCCAAG
ACTGGAGGCACTGTCTCAGACCAGGCGTTGCTCTTTGGTGACGATGATGCTGGTGAAGGG
CCTTCTTCCCTGATCAGGGAGAAACCTGTTCCCAAACAGAATGAGAATGAGGAGGAAAAT
CTTGATAAAGAGCAGACTGGAAATCTAAAACAGGAGTTGGATGACAAAAGCGCCACCTGT
AAGGCATATCCAAAGCGTCCTCTGCTTGGACTGGTTCTGACTCCCACTCGAGAGCTGGCC
GTCCAGGTCAAACAGCACATTGATGCTGTGGCCAGGTTTACAGGAATTAAAACTGCTATT
TTGGTTGGTGGAATGTCCACGCAGAAACAGCAGAGGATGCTGAACCGTCGTCCTGAGATT
GTGGTTGCTACTCCAGGCCGGCTGTGGGAATTAATTAAAGAAAAGCATTATCATTTGAGG
AACCTTCGGCAGCTCAGGTGCCTGGTAGTGGATGAGGCTGACCGGATGGTTGAGAAAGGC
CATTTTGCTGAGCTCTCACAGCTGCTAGAGATGCTCAATGACTCCCAATACAACCCAAAG
AGACAAACGCTTGTTTTTTCTGCCACACTCACCCTGGTGCATCAGGCTCCTGCTCGAATC
CTTCATAAGAAGCACACCAAGAAAATGGATAAAACAGCCAAACTTGACCTCCTTATGCAG
AAAATTGGCATGAGGGGCAAGCCCAAGGTCATTGACCTCACAAGGAATGAGGCCACGGTG
GAGACGCTAACAGAGACCAAGATCCATTGTGAGACTGATGAGAAAGACTTCTACTTGTAC
TACTTCCTGATGCAGTATCCAGGCCGCAGCTTAGTGTTTGCCAACAGTATCTCCTGCATC
AAACGCCTCTCTGGGCTCCTCAAAGTCCTTGATATCATGCCCTTGACCCTGCATGCCTGT
ATGCACCAGAAGCAGAGGCTCAGAAACCTGGAGCAGTTTGCCCGTCTGGAAGACTGTGTT
CTCTTGGCAACAGATGTGGCGGCTCGGGGTCTGGATATTCCTAAAGTCCAGCATGTCATC
CATTACCAGGTCCCACGTACCTCGGAGATTTATGTCCACCGAAGTGGTCGAACTGCTCGA
GCTACCAATGAAGGCCTCAGTCTGATGCTCATTGGGCCTGAGGATGTGATCAACTTTAAG
AAGATTTACAAAACGCTCAAGAAAGATGAGGATATCCCACTGTTCCCCGTGCAGACAAAA
TACATGGATGTGGTCAAGGAGCGAATCCGTTTAGCTCGACAGATTGAGAAATCTGAGTAT
CGGAACTTCCAGGCTTGCCTGCACAACTCTTGGATTGAGCAGGCAGCAGCTGCCCTGGAG
ATTGAGCTGGAAGAAGACATGTATAAGGGAGGAAAAGCTGACCAGCAAGAAGAACGTCGG
AGACAAAAGCAGATGAAGGTTCTGAAGAAGGAGCTGCGCCACCTGCTGTCCCAGCCACTG
TTTACGGAGAGCCAGAAAACCAAGTATCCCACTCAGTCTGGCAAGCCGCCCCTGCTTGTG
TCTGCCCCAAGTAAGAGCGAGTCTGCTTTGAGCTGTCTCTCCAAGCAGAAGAAGAAGAAG
ACAAAGAAGCCGAAGGAGCCACAGCCGGAACAGCCACAGCCAAGTACAAGTGCAAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2664
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DDX24
Symbol Nomenclature : DDX24
Designation : ATP-dependent RNA helicase DDX24|DEAD (Asp-Glu-Ala-Asp) box polypeptide 24|DEAD box protein 24|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24|S. cerevisiae CHL1-like helicase
Full Nomenclature : DEAD (Asp-Glu-Ala-Asp) box helicase 24
GENEID : 57062
HGNC : 13266
MIM : 606181
Vega : OTTHUMG00000171301
Target GenBank: BC008847

 

Reference Sequence Alignment

  

HsCD00329596      ATGAAGTTGAAGGACACAAAATCAAGGCCAAAGCAGTCAAGCTGTGGCAAATTTCAGACA
NM_020414.3       ATGAAGTTGAAGGACACAAAATCAAGGCCAAAGCAGTCAAGCTGTGGCAAATTTCAGACA
                  ************************************************************

HsCD00329596      AAGGGAATCAAAGTTGTGGGAAAATGGAAGGAAGTGAAGATTGACCCAAATATGTTTGCA
NM_020414.3       AAGGGAATCAAAGTTGTGGGAAAATGGAAGGAAGTGAAGATTGACCCAAATATGTTTGCA
                  ************************************************************

HsCD00329596      GATGGACAGATGGATGACTTGGTGTGCTTTGAGGAATTGACAGATTACCAGTTGGTCTCC
NM_020414.3       GATGGACAGATGGATGACTTGGTGTGCTTTGAGGAATTGACAGATTACCAGTTGGTCTCC
                  ************************************************************

HsCD00329596      CCTGCCAAGAATCCCTCCAGTCTCTTCTCAAAGGAAGCACCCAAGAGAAAGGCACAAGCT
NM_020414.3       CCTGCCAAGAATCCCTCCAGTCTCTTCTCAAAGGAAGCACCCAAGAGAAAGGCACAAGCT
                  ************************************************************

HsCD00329596      GTTTCAGAAGAAGAGGAGGAGGAGGAGGGAAAGTCTAGCTCACCAAAGAAAAAGATCAAG
NM_020414.3       GTTTCAGAAGAAGAGGAGGAGGAGGAGGGAAAGTCTAGCTCACCAAAGAAAAAGATCAAG
                  ************************************************************

HsCD00329596      TTGAAGAAAAGTAAAAATGTAGCAACTGAAGGAACCAGTACCCAGAAAGAATTTGAAGTG
NM_020414.3       TTGAAGAAAAGTAAAAATGTAGCAACTGAAGGAACCAGTACCCAGAAAGAATTTGAAGTG
                  ************************************************************

HsCD00329596      AAAGATCCTGAGCTGGAGGCCCAGGGAGATGACATGGTTTGTGATGATCCGGAGGCTGGG
NM_020414.3       AAAGATCCTGAGCTGGAGGCCCAGGGAGATGACATGGTTTGTGATGATCCGGAGGCTGGG
                  ************************************************************

HsCD00329596      GAGATGACATCAGAAAACCTGGTCCAAACTGCTCCAAAAAAGAAGAAAAATAAAGGGAAA
NM_020414.3       GAGATGACATCAGAAAACCTGGTCCAAACTGCTCCAAAAAAGAAGAAAAATAAAGGGAAA
                  ************************************************************

HsCD00329596      AAAGGGTTGGAGCCTTCTCAGAGCACTGCTGCCAAGGTGCCCAAAAAAGCGAAGACATGG
NM_020414.3       AAAGGGTTGGAGCCTTCTCAGAGCACTGCTGCCAAGGTGCCCAAAAAAGCGAAGACATGG
                  ************************************************************

HsCD00329596      ATTCCTGAAGTTCATGATCAGAAAGCAGATGTGTCAGCTTGGAAGGACCTGTTTGTTCCC
NM_020414.3       ATTCCTGAAGTTCATGATCAGAAAGCAGATGTGTCAGCTTGGAAGGACCTGTTTGTTCCC
                  ************************************************************

HsCD00329596      AGGCCGGTTCTCCGAGCACTCAGCTTTCTAGGCTTCTCTGCACCCACACCAATCCAAGCC
NM_020414.3       AGGCCGGTTCTCCGAGCACTCAGCTTTCTAGGCTTCTCTGCACCCACACCAATCCAAGCC
                  ************************************************************

HsCD00329596      CTGACCTTGGCACCTGCCATCCGTGACAAACTGGACATCCTTGGGGCTGCTGAGACAGGA
NM_020414.3       CTGACCTTGGCACCTGCCATCCGTGACAAACTGGACATCCTTGGGGCTGCTGAGACAGGA
                  ************************************************************

HsCD00329596      AGTGGGAAAACTCTTGCCTTTGCCATCCCAATGATTCATGCGGTGTTGCAGTGGCAGAAG
NM_020414.3       AGTGGGAAAACTCTTGCCTTTGCCATCCCAATGATTCATGCGGTGTTGCAGTGGCAGAAG
                  ************************************************************

HsCD00329596      AGGAATGCTGCCCCTCCTCCAAGTAACACCGAAGCACCACCTGGAGAGACCAGAACTGAG
NM_020414.3       AGGAATGCTGCCCCTCCTCCAAGTAACACCGAAGCACCACCTGGAGAGACCAGAACTGAG
                  ************************************************************

HsCD00329596      GCCGGAGCTGAGACTAGATCACCAGGCAAGGCTGAAGCTGAGTCTGATGCATTGCCTGAC
NM_020414.3       GCCGGAGCTGAGACTAGATCACCAGGCAAGGCTGAAGCTGAGTCTGATGCATTGCCTGAC
                  ************************************************************

HsCD00329596      GATACTGTAATTGAGAGTGAAGCACTGCCCAGTGATATTGCAGCCGAGGCCAGAGCCAAG
NM_020414.3       GATACTGTAATTGAGAGTGAAGCACTGCCCAGTGATATTGCAGCCGAGGCCAGAGCCAAG
                  ************************************************************

HsCD00329596      ACTGGAGGCACTGTCTCAGACCAGGCGTTGCTCTTTGGTGACGATGATGCTGGTGAAGGG
NM_020414.3       ACTGGAGGCACTGTCTCAGACCAGGCGTTGCTCTTTGGTGACGATGATGCTGGTGAAGGG
                  ************************************************************

HsCD00329596      CCTTCTTCCCTGATCAGGGAGAAACCTGTTCCCAAACAGAATGAGAATGAGGAGGAAAAT
NM_020414.3       CCTTCTTCCCTGATCAGGGAGAAACCTGTTCCCAAACAGAATGAGAATGAGGAGGAAAAT
                  ************************************************************

HsCD00329596      CTTGATAAAGAGCAGACTGGAAATCTAAAACAGGAGTTGGATGACAAAAGCGCCACCTGT
NM_020414.3       CTTGATAAAGAGCAGACTGGAAATCTAAAACAGGAGTTGGATGACAAAAGCGCCACCTGT
                  ************************************************************

HsCD00329596      AAGGCATATCCAAAGCGTCCTCTGCTTGGACTGGTTCTGACTCCCACTCGAGAGCTGGCC
NM_020414.3       AAGGCATATCCAAAGCGTCCTCTGCTTGGACTGGTTCTGACTCCCACTCGAGAGCTGGCC
                  ************************************************************

HsCD00329596      GTCCAGGTCAAACAGCACATTGATGCTGTGGCCAGGTTTACAGGAATTAAAACTGCTATT
NM_020414.3       GTCCAGGTCAAACAGCACATTGATGCTGTGGCCAGGTTTACAGGAATTAAAACTGCTATT
                  ************************************************************

HsCD00329596      TTGGTTGGTGGAATGTCCACGCAGAAACAGCAGAGGATGCTGAACCGTCGTCCTGAGATT
NM_020414.3       TTGGTTGGTGGAATGTCCACGCAGAAACAGCAGAGGATGCTGAACCGTCGTCCTGAGATT
                  ************************************************************

HsCD00329596      GTGGTTGCTACTCCAGGCCGGCTGTGGGAATTAATTAAAGAAAAGCATTATCATTTGAGG
NM_020414.3       GTGGTTGCTACTCCAGGCCGGCTGTGGGAATTAATTAAAGAAAAGCATTATCATTTGAGG
                  ************************************************************

HsCD00329596      AACCTTCGGCAGCTCAGGTGCCTGGTAGTGGATGAGGCTGACCGGATGGTTGAGAAAGGC
NM_020414.3       AACCTTCGGCAGCTCAGGTGCCTGGTAGTGGATGAGGCTGACCGGATGGTTGAGAAAGGC
                  ************************************************************

HsCD00329596      CATTTTGCTGAGCTCTCACAGCTGCTAGAGATGCTCAATGACTCCCAATACAACCCAAAG
NM_020414.3       CATTTTGCTGAGCTCTCACAGCTGCTAGAGATGCTCAATGACTCCCAATACAACCCAAAG
                  ************************************************************

HsCD00329596      AGACAAACGCTTGTTTTTTCTGCCACACTCACCCTGGTGCATCAGGCTCCTGCTCGAATC
NM_020414.3       AGACAAACGCTTGTTTTTTCTGCCACACTCACCCTGGTGCATCAGGCTCCTGCTCGAATC
                  ************************************************************

HsCD00329596      CTTCATAAGAAGCACACCAAGAAAATGGATAAAACAGCCAAACTTGACCTCCTTATGCAG
NM_020414.3       CTTCATAAGAAGCACACCAAGAAAATGGATAAAACAGCCAAACTTGACCTCCTTATGCAG
                  ************************************************************

HsCD00329596      AAAATTGGCATGAGGGGCAAGCCCAAGGTCATTGACCTCACAAGGAATGAGGCCACGGTG
NM_020414.3       AAAATTGGCATGAGGGGCAAGCCCAAGGTCATTGACCTCACAAGGAATGAGGCCACGGTG
                  ************************************************************

HsCD00329596      GAGACGCTAACAGAGACCAAGATCCATTGTGAGACTGATGAGAAAGACTTCTACTTGTAC
NM_020414.3       GAGACGCTAACAGAGACCAAGATCCATTGTGAGACTGATGAGAAAGACTTCTACTTGTAC
                  ************************************************************

HsCD00329596      TACTTCCTGATGCAGTATCCAGGCCGCAGCTTAGTGTTTGCCAACAGTATCTCCTGCATC
NM_020414.3       TACTTCCTGATGCAGTATCCAGGCCGCAGCTTAGTGTTTGCCAACAGTATCTCCTGCATC
                  ************************************************************

HsCD00329596      AAACGCCTCTCTGGGCTCCTCAAAGTCCTTGATATCATGCCCTTGACCCTGCATGCCTGT
NM_020414.3       AAACGCCTCTCTGGGCTCCTCAAAGTCCTTGATATCATGCCCTTGACCCTGCATGCCTGT
                  ************************************************************

HsCD00329596      ATGCACCAGAAGCAGAGGCTCAGAAACCTGGAGCAGTTTGCCCGTCTGGAAGACTGTGTT
NM_020414.3       ATGCACCAGAAGCAGAGGCTCAGAAACCTGGAGCAGTTTGCCCGTCTGGAAGACTGTGTT
                  ************************************************************

HsCD00329596      CTCTTGGCAACAGATGTGGCGGCTCGGGGTCTGGATATTCCTAAAGTCCAGCATGTCATC
NM_020414.3       CTCTTGGCAACAGATGTGGCAGCTCGGGGTCTGGATATTCCTAAAGTCCAGCATGTCATC
                  ********************.***************************************

HsCD00329596      CATTACCAGGTCCCACGTACCTCGGAGATTTATGTCCACCGAAGTGGTCGAACTGCTCGA
NM_020414.3       CATTACCAGGTCCCACGTACCTCGGAGATTTATGTCCACCGAAGTGGTCGAACTGCTCGA
                  ************************************************************

HsCD00329596      GCTACCAATGAAGGCCTCAGTCTGATGCTCATTGGGCCTGAGGATGTGATCAACTTTAAG
NM_020414.3       GCTACCAATGAAGGCCTCAGTCTGATGCTCATTGGGCCTGAGGATGTGATCAACTTTAAG
                  ************************************************************

HsCD00329596      AAGATTTACAAAACGCTCAAGAAAGATGAGGATATCCCACTGTTCCCCGTGCAGACAAAA
NM_020414.3       AAGATTTACAAAACGCTCAAGAAAGATGAGGATATCCCACTGTTCCCCGTGCAGACAAAA
                  ************************************************************

HsCD00329596      TACATGGATGTGGTCAAGGAGCGAATCCGTTTAGCTCGACAGATTGAGAAATCTGAGTAT
NM_020414.3       TACATGGATGTGGTCAAGGAGCGAATCCGTTTAGCTCGACAGATTGAGAAATCTGAGTAT
                  ************************************************************

HsCD00329596      CGGAACTTCCAGGCTTGCCTGCACAACTCTTGGATTGAGCAGGCAGCAGCTGCCCTGGAG
NM_020414.3       CGGAACTTCCAGGCTTGCCTGCACAACTCTTGGATTGAGCAGGCAGCAGCTGCCCTGGAG
                  ************************************************************

HsCD00329596      ATTGAGCTGGAAGAAGACATGTATAAGGGAGGAAAAGCTGACCAGCAAGAAGAACGTCGG
NM_020414.3       ATTGAGCTGGAAGAAGACATGTATAAGGGAGGAAAAGCTGACCAGCAAGAAGAACGTCGG
                  ************************************************************

HsCD00329596      AGACAAAAGCAGATGAAGGTTCTGAAGAAGGAGCTGCGCCACCTGCTGTCCCAGCCACTG
NM_020414.3       AGACAAAAGCAGATGAAGGTTCTGAAGAAGGAGCTGCGCCACCTGCTGTCCCAGCCACTG
                  ************************************************************

HsCD00329596      TTTACGGAGAGCCAGAAAACCAAGTATCCCACTCAGTCTGGCAAGCCGCCCCTGCTTGTG
NM_020414.3       TTTACGGAGAGCCAGAAAACCAAGTATCCCACTCAGTCTGGCAAGCCGCCCCTGCTTGTG
                  ************************************************************

HsCD00329596      TCTGCCCCAAGTAAGAGCGAGTCTGCTTTGAGCTGTCTCTCCAAGCAGAAGAAGAAGAAG
NM_020414.3       TCTGCCCCAAGTAAGAGCGAGTCTGCTTTGAGCTGTCTCTCCAAGCAGAAGAAGAAGAAG
                  ************************************************************

HsCD00329596      ACAAAGAAGCCGAAGGAGCCACAGCCGGAACAGCCACAGCCAAGTACAAGTGCAAATTTG
NM_020414.3       ACAAAGAAGCCGAAGGAGCCACAGCCGGAACAGCCACAGCCAAGTACAAGTGCAAATTAA
                  **********************************************************:.

Clone

Cloning Information : 269098
HIP Master Clone ID : 169726
Original Clone ID : FLH269098.01L

Organism


TAX_ID : 9606
Species Specific ID: 57062

Genome

Chromosome : 14
Map Location : 14q32
Ensembl : ENSG00000089737

Reagents

Labome : DDX24-antibody