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JUB (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AJUBA
Gene Name:  ajuba LIM protein
Sequence             
Original Clone ID: FLH269103.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329601

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1617nts         Open reading frame : 1 to 1617
Reference Sequence Alignment

ATGGAGCGGTTAGGAGAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGT
GAATCTAGCCGGTCTGGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGG
TTGGGGGGACCTAGGAAGTCAGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCG
TTGGAGCCGGCCCGGGAGCAAGGTTCCCTGGACGCTGAGCGAAATCAGCGCGGCTCCTTT
GAGGCGCCGCGCTACGAAGGCTCTTTTCCCGCGGGGCCGCCGCCCACCCGGGCCTTGCCT
CTACCTCAGTCGTTGCCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGCCCTCAGCCCC
CGCTCTAGCTTCGCCAGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGC
AGCCTGCTGCTGGACGGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAAT
CGCACCAGCGGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGG
CCGTGCCTGTTTGGCCCACCCCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGAGGGGTC
CCGTCCGCCTACCCGGAGCTCCACGCCGCCCTGGACCGATTGTACGCTCAGCGGCCCGCG
GGGTTCGGCTGCCAGGAAAGCCGCCACTCGTATCCCCCGGCCCTGGGCAGCCCTGGAGCT
CTAGCCGGGGCCGGAGTGGGAGCGGCGGGGCCCTTGGAGAGACGGGGGGCGCAACCCGGA
CGACACTCTGTGACCGGCTACGGGGACTGCGCCGTGGGCGCCCGGTACCAGGACGAGCTA
ACAGCTTTGCTTCGCCTGACGGTGGGCACCGGTGGGCGAGAAGCCGGAGCCCGCGGAGAA
CCCTCGGGGATTGAGCCGTCGGGTCTGGAGGAGCCACCAGGTCCTTTCGTTCCGGAGGCC
GCCCGGGCCCGGATGCGGGAGCCAGAGGCCAGGGAGGACTACTTCGGCACCTGTATCAAG
TGCAACAAAGGCATCTATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCAC
ACCCAGTGCTTTGTTTGCTGCTCTTGTGGGCGAACTTTGCGTTGCAAGGCTTTCTACAGT
GTCAATGGCTCTGTGTACTGTGAGGAAGATTATCTGTTTTCAGGGTTTCAGGAGGCAGCT
GAGAAATGCTGTGTCTGTGGTCACTTGATTTTGGAGAAGATCCTACAAGCAATGGGGAAG
TCCTATCATCCAGGCTGTTTCCGATGCATTGTTTGCAACAAGTGCCTGGATGGCATCCCC
TTCACAGTGGACTTCTCCAACCAAGTATACTGTGTCACCGACTACCACAAAAATTATGCT
CCTAAGTGTGCAGCCTGTGGCCAACCCATCCTCCCCTCTGAGGGCTGTGAGGACATCGTG
AGGGTGATATCCATGGACCGGGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGG
ATGCAGCTGAGTGATGAGGAAGGCTGCTGCTGTTTCCCTCTGGATGGGCACTTGCTCTGC
CATGGTTGCCACATGCAGCGGCTCAATGCCCGACAACCCCCTGCCAACTATATCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1689
Start on reference sequence 73
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AJUBA
Symbol Nomenclature : AJUBA
SYNONYM : JUB
Designation : LIM domain-containing protein ajuba|jub, ajuba homolog
Full Nomenclature : ajuba LIM protein
GENEID : 84962
HGNC : 20250
MIM : 609066
Vega : OTTHUMG00000028711
Target GenBank: BC007580

 

Reference Sequence Alignment

  

HsCD00329601      ATGGAGCGGTTAGGAGAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGT
NM_032876.5       ATGGAGCGGTTAGGAGAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGT
                  ************************************************************

HsCD00329601      GAATCTAGCCGGTCTGGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGG
NM_032876.5       GAATCTAGCCGGTCTGGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGG
                  ************************************************************

HsCD00329601      TTGGGGGGACCTAGGAAGTCAGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCG
NM_032876.5       TTGGGGGGACCTAGGAAGTCAGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCG
                  ************************************************************

HsCD00329601      TTGGAGCCGGCCCGGGAGCAAGGTTCCCTGGACGCTGAGCGAAATCAGCGCGGCTCCTTT
NM_032876.5       TTGGAGCCGGCCCGGGAGCAAGGTTCCCTGGACGCTGAGCGAAATCAGCGCGGCTCCTTT
                  ************************************************************

HsCD00329601      GAGGCGCCGCGCTACGAAGGCTCTTTTCCCGCGGGGCCGCCGCCCACCCGGGCCTTGCCT
NM_032876.5       GAGGCGCCGCGCTACGAAGGCTCTTTTCCCGCGGGGCCGCCGCCCACCCGGGCCTTGCCT
                  ************************************************************

HsCD00329601      CTACCTCAGTCGTTGCCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGCCCTCAGCCCC
NM_032876.5       CTACCTCAGTCGTTGCCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGCCCTCAGCCCC
                  ************************************************************

HsCD00329601      CGCTCTAGCTTCGCCAGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGC
NM_032876.5       CGCTCTAGCTTCGCCAGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGC
                  ************************************************************

HsCD00329601      AGCCTGCTGCTGGACGGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAAT
NM_032876.5       AGCCTGCTGCTGGACGGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAAT
                  ************************************************************

HsCD00329601      CGCACCAGCGGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGG
NM_032876.5       CGCACCAGCGGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGG
                  ************************************************************

HsCD00329601      CCGTGCCTGTTTGGCCCACCCCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGAGGGGTC
NM_032876.5       CCGTGCCTGTTTGGCCCACCCCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGAGGGGTC
                  ************************************************************

HsCD00329601      CCGTCCGCCTACCCGGAGCTCCACGCCGCCCTGGACCGATTGTACGCTCAGCGGCCCGCG
NM_032876.5       CCGTCCGCCTACCCGGAGCTCCACGCCGCCCTGGACCGATTGTACGCTCAGCGGCCCGCG
                  ************************************************************

HsCD00329601      GGGTTCGGCTGCCAGGAAAGCCGCCACTCGTATCCCCCGGCCCTGGGCAGCCCTGGAGCT
NM_032876.5       GGGTTCGGCTGCCAGGAAAGCCGCCACTCGTATCCCCCGGCCCTGGGCAGCCCTGGAGCT
                  ************************************************************

HsCD00329601      CTAGCCGGGGCCGGAGTGGGAGCGGCGGGGCCCTTGGAGAGACGGGGGGCGCAACCCGGA
NM_032876.5       CTAGCCGGGGCCGGAGTGGGAGCGGCGGGGCCCTTGGAGAGACGGGGGGCGCAACCCGGA
                  ************************************************************

HsCD00329601      CGACACTCTGTGACCGGCTACGGGGACTGCGCCGTGGGCGCCCGGTACCAGGACGAGCTA
NM_032876.5       CGACACTCTGTGACCGGCTACGGGGACTGCGCCGTGGGCGCCCGGTACCAGGACGAGCTA
                  ************************************************************

HsCD00329601      ACAGCTTTGCTTCGCCTGACGGTGGGCACCGGTGGGCGAGAAGCCGGAGCCCGCGGAGAA
NM_032876.5       ACAGCTTTGCTTCGCCTGACGGTGGGCACCGGTGGGCGAGAAGCCGGAGCCCGCGGAGAA
                  ************************************************************

HsCD00329601      CCCTCGGGGATTGAGCCGTCGGGTCTGGAGGAGCCACCAGGTCCTTTCGTTCCGGAGGCC
NM_032876.5       CCCTCGGGGATTGAGCCGTCGGGTCTGGAGGAGCCACCAGGTCCTTTCGTTCCGGAGGCC
                  ************************************************************

HsCD00329601      GCCCGGGCCCGGATGCGGGAGCCAGAGGCCAGGGAGGACTACTTCGGCACCTGTATCAAG
NM_032876.5       GCCCGGGCCCGGATGCGGGAGCCAGAGGCCAGGGAGGACTACTTCGGCACCTGTATCAAG
                  ************************************************************

HsCD00329601      TGCAACAAAGGCATCTATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCAC
NM_032876.5       TGCAACAAAGGCATCTATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCAC
                  ************************************************************

HsCD00329601      ACCCAGTGCTTTGTTTGCTGCTCTTGTGGGCGAACTTTGCGTTGCAAGGCTTTCTACAGT
NM_032876.5       ACCCAGTGCTTTGTTTGCTGCTCTTGTGGGCGAACTTTGCGTTGCAAGGCTTTCTACAGT
                  ************************************************************

HsCD00329601      GTCAATGGCTCTGTGTACTGTGAGGAAGATTATCTGTTTTCAGGGTTTCAGGAGGCAGCT
NM_032876.5       GTCAATGGCTCTGTGTACTGTGAGGAAGATTATCTGTTTTCAGGGTTTCAGGAGGCAGCT
                  ************************************************************

HsCD00329601      GAGAAATGCTGTGTCTGTGGTCACTTGATTTTGGAGAAGATCCTACAAGCAATGGGGAAG
NM_032876.5       GAGAAATGCTGTGTCTGTGGTCACTTGATTTTGGAGAAGATCCTACAAGCAATGGGGAAG
                  ************************************************************

HsCD00329601      TCCTATCATCCAGGCTGTTTCCGATGCATTGTTTGCAACAAGTGCCTGGATGGCATCCCC
NM_032876.5       TCCTATCATCCAGGCTGTTTCCGATGCATTGTTTGCAACAAGTGCCTGGATGGCATCCCC
                  ************************************************************

HsCD00329601      TTCACAGTGGACTTCTCCAACCAAGTATACTGTGTCACCGACTACCACAAAAATTATGCT
NM_032876.5       TTCACAGTGGACTTCTCCAACCAAGTATACTGTGTCACCGACTACCACAAAAATTATGCT
                  ************************************************************

HsCD00329601      CCTAAGTGTGCAGCCTGTGGCCAACCCATCCTCCCCTCTGAGGGCTGTGAGGACATCGTG
NM_032876.5       CCTAAGTGTGCAGCCTGTGGCCAACCCATCCTCCCCTCTGAGGGCTGTGAGGACATCGTG
                  ************************************************************

HsCD00329601      AGGGTGATATCCATGGACCGGGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGG
NM_032876.5       AGGGTGATATCCATGGACCGGGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGG
                  ************************************************************

HsCD00329601      ATGCAGCTGAGTGATGAGGAAGGCTGCTGCTGTTTCCCTCTGGATGGGCACTTGCTCTGC
NM_032876.5       ATGCAGCTGAGTGATGAGGAAGGCTGCTGCTGTTTCCCTCTGGATGGGCACTTGCTCTGC
                  ************************************************************

HsCD00329601      CATGGTTGCCACATGCAGCGGCTCAATGCCCGACAACCCCCTGCCAACTATATCTTG
NM_032876.5       CATGGTTGCCACATGCAGCGGCTCAATGCCCGACAACCCCCTGCCAACTATATCTGA
                  ******************************************************* .

Pathways

NCI : Aurora A signaling
NCI : E-cadherin signaling in keratinocytes
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : G2/M Transition
Reactome : Mitotic G2-G2/M phases
Reactome : Regulation of PLK1 Activity at G2/M Transition

Protein

SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : A8MX18
UniProt : Q96IF1
HPRD : 16426

Clone

Cloning Information : 269103
HIP Master Clone ID : 169478
Original Clone ID : FLH269103.01L

Organism


TAX_ID : 9606
Species Specific ID: 84962

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000129474

Reagents

Labome : Ajuba-antibody